Hasil untuk "physics.soc-ph"

Niels Holten-Andersen, Matthew J. Harrington, H. Birkedal et al.

F. Zeng, Shafaqat Ali, Haitao Zhang et al.

Ying-hong Guan, Jun Ma, Xu-chun Li et al.

Junyan Han, K. Burgess

Congcong Shen, J. Xiong, Huayong Zhang et al.

J. Rousk, P. Brookes, E. Bååth

G. Nicol, S. Leininger, C. Schleper et al.

Y. Al-Degs, M. El‐Barghouthi, A. El‐Sheikh et al.

K. Caldeira, M. Wickett, P. Duffy et al.

P. Gupta, K. Vermani, S. Garg

G. Helmlinger, F. Yuan, M. Dellian et al.

Kateryna Zhalnina, Raquel Dias, Patrícia Dörr de Quadros et al.

N. Tanner, Yinhua Zhang, T. C. Evans

Nucleic acid amplification is the basis for many molecular diagnostic assays. In these cases, the amplification product must be detected and analyzed, typically requiring extended workflow time, sophisticated equipment, or both. Here we present a novel method of amplification detection that harnesses the pH change resulting from amplification reactions performed with minimal buffering capacity. In loop-mediated isothermal amplification (LAMP) reactions, we achieved rapid (<30 min) and sensitive (<10 copies) visual detection using pH-sensitive dyes. Additionally, the detection can be performed in real time, enabling high-throughput or quantitative applications. We also demonstrate this visual detection for another isothermal amplification method (strand-displacement amplification), PCR, and reverse transcription LAMP (RT-LAMP) detection of RNA. The colorimetric detection of amplification presented here represents a generally applicable approach for visual detection of nucleic acid amplification, enabling molecular diagnostic tests to be analyzed immediately without the need for specialized and expensive instrumentation.

Chuanxi Wang, zhenzhu xu, Hao Cheng et al.

M. Koziolek, M. Grimm, D. Becker et al.

Christopher C. Deng, William L. A. Brooks, K. Abboud et al.

W. Guo, Chun-hua Lu, R. Orbach et al.

Špela Zupančič, Z. Lavrič, J. Kristl

Katherine M. Strickler, A. Fremier, C. Goldberg

Shiyi Zhang, Andrew M Bellinger, D. Glettig et al.

Devices resident in the stomach -- which are used for a variety of clinical applications including nutritional modulation for bariatrics, ingestible electronics for diagnosis and monitoring, and gastric retentive dosage forms for prolonged drug delivery -- typically incorporate elastic polymers to compress the devices during delivery through the esophagus and other narrow orifices in the digestive system. However, in the event of accidental device fracture or migration, the non-degradable nature of these materials risks intestinal obstruction. Here, we show that an elastic, pH-responsive supramolecular gel remains stable and elastic in the acidic environment of the stomach but can be dissolved in the neutral-pH environment of the small and large intestines. In a large animal model, prototype devices with these materials as the key component demonstrated prolonged gastric retention and safe passage. These enteric elastomers should increase the safety profile for a wide range of gastric retentive devices.