In human societies, cultural norms arise when behaviours are transmitted through social networks via high-fidelity social learning. However, a paucity of experimental studies has meant that there is no comparable understanding of the process by which socially transmitted behaviours might spread and persist in animal populations. Here we show experimental evidence of the establishment of foraging traditions in a wild bird population. We introduced alternative novel foraging techniques into replicated wild sub-populations of great tits (Parus major) and used automated tracking to map the diffusion, establishment and long-term persistence of the seeded innovations. Furthermore, we used social network analysis to examine the social factors that influenced diffusion dynamics. From only two trained birds in each sub-population, the information spread rapidly through social network ties, to reach an average of 75% of individuals, with a total of 414 knowledgeable individuals performing 57,909 solutions over all replicates. The sub-populations were heavily biased towards using the technique that was originally introduced, resulting in established local traditions that were stable over two generations, despite a high population turnover. Finally, we demonstrate a strong effect of social conformity, with individuals disproportionately adopting the most frequent local variant when first acquiring an innovation, and continuing to favour social information over personal information. Cultural conformity is thought to be a key factor in the evolution of complex culture in humans. In providing the first experimental demonstration of conformity in a wild non-primate, and of cultural norms in foraging techniques in any wild animal, our results suggest a much broader taxonomic occurrence of such an apparently complex cultural behaviour.
Outbreaks of Ebola hemorrhagic fever in sub-Saharan Africa are associated with case fatality rates of up to 90%. Currently, neither a vaccine nor an effective antiviral treatment is available for use in humans. Here, we evaluated the efficacy of the pyrazinecarboxamide derivative T-705 (favipiravir) against Zaire Ebola virus (EBOV) in vitro and in vivo. T-705 suppressed replication of Zaire EBOV in cell culture by 4log units with an IC90 of 110μM. Mice lacking the type I interferon receptor (IFNAR(-)(/)(-)) were used as in vivo model for Zaire EBOV-induced disease. Initiation of T-705 administration at day 6 post infection induced rapid virus clearance, reduced biochemical parameters of disease severity, and prevented a lethal outcome in 100% of the animals. The findings suggest that T-705 is a candidate for treatment of Ebola hemorrhagic fever.
Manuel Collado-Cuadrado, Claudia Alarcón-Torrecillas, Alfonso Balmori-de la Puente
et al.
Heartworm disease, caused by <i>Dirofilaria immitis,</i> is a vector-borne zoonotic disease, (mainly affecting canids and felids) causing chronic vascular and pulmonary pathology in its early stages, which worsens with parasite load and/or death of adult worms in the pulmonary artery or right heart cavity, and can be fatal to the host. Angiogenesis is a mechanism by which new blood vessels are formed from existing ones. The aim of this work was to study the effect of two molecules of the <i>D. immitis</i> excretory/secretory antigen (DiES) on the angiogenic process, taking into account that this antigen is able to interact with this process and use it as a survival mechanism. For this purpose, an in vitro model of endothelial cells was used and treated with two recombinant proteins, i.e., actin (Act) and fructose-bisphosphate aldolase (FBAL) proteins belonging to DiES, and both pro- and antiangiogenic molecules were analyzed, as well as the cellular processes of cell proliferation, migration, and pseudocapillary formation. Act and FBAL proteins, together with vascular endothelial growth factor (VEGF-A), as an angiogenic precursor, are able to stimulate the production of proangiogenic factors as well as cellular processes of proliferation, migration, and pseudocapillary formation. This implies that these molecules could be produced by <i>D. immitis</i> to facilitate its survival, and the relationship between parasite and canine host would be further elaborated.
Darman Agung Sandakila, Gracetia Devita Hadi, Riska Gultom
et al.
The Black Soldier Fly (Hermetia illucens L.) or BSF is one of a group of insects that has a length of 15-20 mm originating from America. The larvae of the BSF has a high protein content (44.26%), which has great potential in treating wound healing. This study investigated the wound healing potential of Black Soldier Fly Larvae (BSFL) ethanolic extract on mice. The research aimed to determine the extract's content, its effect on wound closure percentage, and its impact on total DNA content in mice. Using a completely randomized design, 30 male mice were divided into 6 treatment groups: no treatment (K-1), placebo (K-2), povidone iodine 10% (K+), and BSFL ethanolic extract at 10%, 15%, and 20% concentrations. Wounds were treated for 14 days, with measurements taken every 2 days. Results showed that the 20% BSFL extract was most effective in wound healing, with wound lengths comparable to the povidone iodine group. The highest total DNA content was observed in the 20% BSFL group (42.25 ± 7.45 µg/mL), indicating enhanced cell proliferation. Gas Chromatography Mass Spectrophotometry analysis revealed high lauric acid content (44.27%) in the extract, known for its antibacterial properties. Organoleptic and homogeneity tests confirmed the ointment's suitability for topical application. The extract's efficacy is attributed to its high protein content, various amino acids, and minerals that play crucial roles in wound healing processes. The study concludes that BSFL ethanolic extract, particularly at 20% concentration, shows promising potential as a natural wound healing agent, offering antibacterial and tissue regeneration properties. These findings open new avenues for developing sustainable, insect-based wound healing treatments.
Golnor Kosari, Mohammad Ali Norouzian, Behzad Khorrami
et al.
This study investigated the potential impact of feeding whole plant basil on sperm quality and the concentration of certain reproductive hormones in male lambs. A total of 18 Zandi male lambs with an initial weight of 28.8 ± 2.03 kg were included in a completely randomized design with three treatments and six repetitions. The experimental treatments included: 1) control (basal diet without basil), 2) diet containing 12.5 % basil, and 3) diet containing 25 % basil. The results showed that feeding basil to male lambs significantly increased testosterone concentration and decreased blood cortisol levels (P < 0.05). Additionally, feeding high levels of basil significantly improved sperm concentration, motility, and viability in the experimental samples, while reducing the level of complete abnormalities and malondialdehyde concentration (P < 0.05). The findings suggest that dietary supplementation of 25 % whole plant basil could be a useful strategy to improve sperm quality and increase testosterone secretion while reducing cortisol levels in male lambs.
Forough Ataollahi, John W. Piltz, Geoff R. Casburn
et al.
This study compared the effect of four levels of K Humate S100R (potassium humate) supplementation on the quality, shelf-life, and nutritional properties of beef. Angus steers (n = 40) were individually housed and fed either 0, 35, 70, or 140 g K Humate S100R/animal/day for 100 days, following a 30 day adjustment period. The steers were slaughtered at the completion of the feeding study. The left m. longissimus lumborum (LL) was collected at 24 h post-mortem and aged for either 2 or 6 weeks before analysis. K Humate S100R supplementation did not affect beef drip loss, cooking loss, shear force, sarcomere length, ultimate pH, intramuscular fat content, or total volatile basic nitrogen concentrations (P > 0.05). Steers supplemented with 70 g/day K Humate S100R produced beef with higher a* values on Days 1 and 3 of retail display (P < 0.05). Beef mineral composition was unchanged by K Humate S100R supplementation (P > 0.05), but there were minor changes to the fatty acid profile. Specifically, the ratio of omega-6 to omega-3 (P < 0.05) and C20:2n-6 concentrations (P < 0.05) increased with supplementation level. Together, these results demonstrate no detrimental effects on beef quality and shelf-life as a result of K Humate S100R supplementation.
Lamiaa Hassan, Ibrahem M. A. Hasan, Zeinab Al-Amgad
et al.
Copper oxide nanoparticles (CuO NPs) are metallic nanoparticles fulfilling several functions such
as good conductivity, catalyst, used in sensors and energy storage devices, and antibacterial
characteristics. However, the cytotoxicity and particular mechanisms of exposure to CuO NPs on
male testicular function are still elusive. In the current study, seventy-five mature male albino rats
received single doses of 0, 100, 200, 1000, and 2000 mg/kg CuO NPs by oral gavage. Blood and
epididymal semen as well as testicular tissue were collected 2, 8, and 15 days after administration.
Serum testosterone level, sperm motility, count, morphology, viability, and gonadosomatic index
(GSI) were assessed at the same time; histological structure of the testes was examined. The result
revealed that CuO NPs significantly reduced serum testosterone levels, suppressed sperm
concentration, and significantly elevated abnormal and dead sperm percent. Furthermore, testicular
tissue showed degeneration of germ, Sertoli, Leydig cells, and spermatocytes with the incidence of
vacuolation and inflammatory cell infiltration. In conclusion, CuO NPs exert adverse and
irreversible effects on testicular function and sperm physiological characteristics; these harmful
effects were markedly observed after administration of high doses of CuO NPs.
Andrea Mazzatenta, Serena Fiorito, Roberto Guadagnini
et al.
The evanescent and invisible communication carried by chemical signals, pheromones, or signature mixtures or, as we prefer, the pheromonal individual fingerprint, between members of the same species is poorly studied in mammals, mainly because of the lack of identification of the molecules. The difference between pheromones and the pheromonal individual fingerprint is that the former generate stereotyped innate responses while the latter requires learning, i.e., different receivers can learn different signature mixtures from the same individual. Furthermore, pheromones are usually produced by a particular gland, while the pheromonal individual fingerprint is the entire bouquet produced by the entire secreting gland of the body. In the present study, we aim to investigate the pheromonal individual fingerprint of brown bears in northern Italy. We collected the entire putative pheromone bouquet from all production sites in free-ranging bears and analyzed the entire crude extract to profile the individual fingerprint according to species-, sex- and subjective-specific characteristics. We were able to putatively characterize the brown bears’ pheromonal individual fingerprints and compare them with the partial pheromone identifications published by other studies. This work is a step forward in the study of the complexity of chemical communication, particularly in a solitary endangered species.
Abstract New morphological, morphometric and scanning electron microscopy data of a nematode of the family Anisakidae, recovered from a specimen of Kogia sima, a cetacean that died off the northern coast of Brazil, are presented in this paper. Morphological features such as the violin-shaped ventricle and short and equal spicules, as well as the distribution of post-cloacal papillae and specificity for the definitive host (Kogiidae cetaceans) demonstrate similarity to Skrjabinisakis paggiae. This research records Kogia sima and S. paggiae on the estuarine coast of Pará, northern Brazil.
SUMMARY: Re-evaluating management practices to highlight opportunities for improving bird welfare is a necessary response to changing genetics, evolving standards, and increasing consumer interest in how poultry is raised. The aim of this study was to evaluate the impact of stocking density (SD) on the welfare and productivity of broilers. The SD treatments were 31, 34.5, 38, and 41.5 kg/m2. Litter moisture decreased and footpad dermatitis improved as SD was reduced, indicating an improvement in welfare. Results from the novel object test suggest birds reared at lower SD were less fearful. Heterophil/lymphocyte ratios were highest in the 31 and 41.5 kg/m2 treatments, indicating these birds experienced more chronic stress. At 20 d as SD was decreased, birds spent more time at the drinker and less time resting. When older (31 d), birds preened more in the 31 and 41.5 kg/m2 treatments. Stretching behavior decreased while ruffling increased at lower SD. Final body weights were heaviest in the 31 and 34.5 kg/m2 treatments. Overall, body weight gain increased as SD was reduced, and feed intake was greatest in the 34.5 kg/m2 treatment. From 21 to 24 d, infectious mortality was reduced in the low SD treatments. The impacts of SD are dependent on environmental management; however, even under research conditions, differences between treatments were observed. The results of this study suggest that reducing SD for broilers results in improved welfare and performance as evidenced by reductions in litter moisture, footpad dermatitis, fear, stress, and infectious mortality as well as greater market body weights and feed consumption.
Anne-Marieke C. Smid, Vanessa Boone, Melanie Jarbeau
et al.
ABSTRACT: Dairy cows are highly motivated to access pasture and have a partial preference for alternative forms of outdoor access (e.g., deep-bedded outdoor sand or wood-chip packs). In addition, Canadians value the provision of outdoor access to dairy cows as they perceive it as important for good cow welfare. In contrast to Europe, Oceania, and the United States, little data exist on the use of outdoor access on Canadian dairy farms. Therefore, our objective was to assess current outdoor access practices for dairy cows in Canada. An online questionnaire was used to determine housing and outdoor access practices for lactating cows, dry cows, pregnant heifers, and weaned, nonpregnant heifers on Canadian dairy farms. The questionnaire was distributed by the 10 provincial milk boards between November 2020 and August 2021, resulting in an 8.9% response rate (n = 903 completed questionnaires). In total, 75% (n = 675) of respondents provided some form of outdoor access to at least 1 cattle class on their farm. Pasture was the most frequently used form of outdoor access for all cattle classes. Based on a weighted average, a total of 29% and 48% of Canadian dairy farms provided lactating and dry cows, respectively, access to pasture; for youngstock, these numbers were 48% and 27% for pregnant heifers and weaned, nonpregnant heifers, respectively. Herd size (for each cow class), indoor housing system, and region were all associated with the provision of pasture. Farms with larger lactating herds less often provided access to pasture; larger herd sizes in terms of weaned, nonpregnant heifers, pregnant heifers, and dry cows were also associated with a lower likelihood of access to pasture. Farms using indoor bedded pack housing for their lactating cows more often provided pasture to this cattle class than farms with freestall or tiestall housing; this likelihood did not differ between farms with tie or freestall housing for this cattle class. Dry cows or pregnant heifers housed in a tiestall were more often provided pasture than freestall-housed dry cows or pregnant heifers. Housing type for weaned, nonpregnant heifers was not associated with the likelihood of pasture provision. Farms in British Columbia or on Canada's East Coast (i.e., Nova Scotia, New Brunswick, and Prince Edward Island) more often provided lactating cows pasture compared with farms in other regions. For the other 3 cattle classes, farms on the East Coast of Canada more often provided pasture than farms in other parts of Canada. These results will inform future decisions regarding outdoor access for Canadian dairy cattle and may also aid in identifying future areas of research. For example, our results may aid in designing housing systems that facilitate outdoor access in larger herds and in areas that are subject to more extreme weather conditions.
ABSTRACT: The objective of this experiment was to investigate the effect of dietary levels of digestible histidine (dHis) and MP on lactational performance and plasma and muscle concentrations of free AA in dairy cows. A randomized block design experiment was conducted with 48 Holstein cows, including 20 primiparous, averaging (±SD) 103 ± 22 d in milk and 45 ± 9 kg/d milk yield at the beginning of the experiment. A 2-wk covariate period preceded 12 experimental wk, of which 10 wk were for data and sample collection. Experimental treatments were (1) MP-adequate (MPA) diet with 2.1% dHis of MP (MPA2.1), (2) MPA with 3.0% dHis (MPA3.0), (3) MP-deficient (MPD) diet with 2.1% dHis (MPD2.1), and (4) MPD with 3.0% dHis (MPD3.0). Actual dHis supply was estimated at 64, 97, 57, and 88 g/d, respectively. Diets supplied MP at 110% (MPA) and 96% (MPD) of NRC 2001 dairy model requirements calculated based on DMI and production data during the experiment. Dry matter intake and milk yield data were collected daily, milk samples for composition and blood samples for AA analysis were collected every other week, and muscle biopsies at the end of covariate period, and during wk 12 of the experiment. The overall DMI was not affected by dHis or MP level. Milk yield tended to be increased by 3.0% dHis compared with 2.1% dHis. Milk true protein concentration and yield were not affected by treatments, whereas milk urea nitrogen concentration was lower for MPD versus the MPA diet. Milk fat concentration was lower for MPD versus MPA. There was a MP × dHis interaction for milk fat yield and energy-corrected milk; milk fat was lower for MPD3.0 versus MPD2.1, but similar for cows fed the MPA diet regardless of dHis level whereas energy-corrected milk was greater for MPA3.0 versus MPA2.1 but tended to be lower for MPD3.0 versus MPD2.1. Plasma His concentration was greater for cows fed dHis3.0, and concentration of sum of essential AA was greater, whereas carnosine, 1-Methyl-His and 3-Methyl-His concentrations were lower for cows fed MPA versus MPD diet. Muscle concentration of His was greater for cows fed dHis3.0 treatment. The apparent efficiency of His utilization was increased at lower MP and His levels. Overall, cows fed a corn silage-based diet supplying MP at 110% of NRC (2001) requirements tended to have increased ECM yield and similar milk protein yield to cows fed a diet supplying MP at 96% of requirements. Supplying dHis at 3.0% of MP (or 86 and 96 g/d, for MPD3.0 and MPA3.0, respectively) tended to increase milk yield and increased plasma and muscle concentrations of His but had minor or no effects on other production variables in dairy cows.
The reliance on animals in children’s literature over the past two centuries has become a key means by which the civilizing process that children go through has been mediated by the animal body. Children are asked both implicitly and explicitly to identify with animals, but then to position themselves as distinctly human through the mode of their interactions with both lived animals and those depicted in literature and film. This core question of identity formation – child/adult, animal/human – forms the foundation of my dissertation, which investigates the overlapping, double-sided rhetorics addressing children, childhood and animals. My dissertation is organized into five areas of interest that pose complementary questions regarding the way in which relationships between animals and children inform and underscore adults’ lived relationships with both of them. Posthumanist scholarship, then, becomes a key means by which to de-prioritize a conception of an exclusively human subjectivity. Cary Wolfe in particular has recently worked to criticize liberal humanism and find ways to push cultural analysis beyond its inherent anthropocentrism in order to combat institutionalized speciesism, which continues to prioritize human beings, thereby excusing the exploitation or extermination of other species. What has been notably overlooked in posthumanism’s challenge to anthropocentric human liberalism, however, is how the human is encultured through literature geared specifically towards a child audience. By examining culturally significant and widely popular works of children’s culture through a posthumanist, or animality studies lens, I argue that Western philosophy’s objective to establish a notion of an exclusively human subjectivity is continually countered in the very texts that ostensibly work to configure human identity. Literature geared toward a child audience reflects and contributes to the cultural tensions created by the oscillation between upholding and undermining the divisions between the human and the animal. My dissertation focuses on the ways in which these works present the boundary between humans and animals as, at best, permeable and in a state of continual flux.
V.A. Gushchina, А.А. Volodkin, N.I. Ostroborodova
et al.
Currently, an urgent task is to find environmentally friendly and economically accessible energy sources for multi-purpose use. One such resource is a perennial plant from the bluegrass family, Miscanthus giganteus, which has a significant increase in above-ground mass by C4-photosynthesis, but cannot realize its potential as a crop with high rates of photosynthetic activity, because in the year of planting it competes poorly with weeds. Therefore, we studied ways to control the weed component in the agrocenosis of miscanthus on the light gray soil of the collection area of Penza State Agrarian University. In the years of the research, characterized by different hydrothermal conditions (HTC 0.64-1.29), it was found that the herbicides of systemic action Balerina and Magnum in the background of Tornado 500 contribute to an increase in the leaf area of miscanthus in the first year up to 18.36...19.09 thousand m2/ha, in the photosynthetic potential to 496.1...508.7 thousand m2∙day/ha. The maximum values of net productivity of photosynthesis (0.62...0.85 g/m2∙day) were observed at the end of June. Due to the double chemical weeding the decrease of weeds leads to the maximum yield of above-ground mass – 8.92...9.09 t/ha. The highest dry matter yield of 2.13...2.97 t/ha was obtained using the Tornado 500 in combination with the herbicides Balerina and Magnum.
Squamous cell carcinoma (SCC) is the most common equine sinonasal and feline oral tumour. This study aimed to describe the computed tomographic and histopathological characteristics of equine and feline SCC. Thirteen horses and 10 cats that had been histopathologically diagnosed with oral or sinonasal SCC and had undergone computed tomography (CT) of the head were retrospectively included in the study. CT characteristics of the mass and involved structures were noted. Histological examinations were evaluated according to a human malignancy grading system for oral SCC, which considered four grades of increasing aggressiveness. In horses, the masses were at the levels of the paranasal sinuses (n = 8), mandible (n = 3), tongue (n = 1), and nasal cavity (n = 1). In cats, the masses were at the levels of the maxilla (n = 4), mandible (n = 3), tongue (n = 1), and buccal region (n = 1) and were diffusely distributed (facial and cranial bones; n = 1). Masses in the equine paranasal sinuses showed only mild, solid/laminar, periosteal reactions with variable cortical destruction. However, maxillary lesions in cats showed severe cortical destruction and irregular, amorphous/pumice stone-like, periosteal reactions. CT revealed different SCC phenotypes that were unrelated to the histological grade. For morphologic parameters of the tumour cell population, a variability for the degree of keratinization and number of mitotic cells was noted in horses and cats. Concerning the tumour-host relationship a marked, extensive and deep invasion into the bone in the majority of horses and cats was seen. Most cases in both the horses and cats were categorized as histological grade III (n = 8); four horses and one cat were categorized as grade IV, and one horse and one cat were categorized as grade II. In this study, we examined the diagnostic images and corresponding applied human histopathological grading of SCC to further elucidate the correlations between pathology and oral and sinonasal SCC imaging in horses and cats.
Biotechnology has taken two directions in efforts to speed up animal production above the rates achievable by selective breeding. Recombinant DNA methods have been used to engineer protein gene products for direct administration to livestock, as in recombinant growth hormone to stimulate lactation in dairy cows or yield faster growing, leaner carcasses in meat animals. Cloned cellulolytic genes have been inserted into ruminal microorganisms with a view to improving ruminant nutrition. The other direction is to use advanced breeding technologies to enhance performance. These include laboratory culture of large numbers of viable embryos for nonsurgical transfer to surrogate mothers, development of methods for sexing sperm and embryos, cloning embryos by nuclear transplantation and gene transfer to create livestock with superior performance traits. In all cases material progress will depend upon a deeper understanding of the underlying physiological and developmental control mechanisms and public confidence that due regard is being paid to animal welfare, and to social and environmental implications.