Effects of dietary basil (Ocimum basilicum) supplementation on reproductive hormones, semen parameters, and testicular development in Zandi male lambs

This study investigated the potential impact of feeding whole plant basil on sperm quality and the concentration of certain reproductive hormones in male lambs. A total of 18 Zandi male lambs with an initial weight of 28.8 ± 2.03 kg were included in a completely randomized design with three treatments and six repetitions. The experimental treatments included: 1) control (basal diet without basil), 2) diet containing 12.5 % basil, and 3) diet containing 25 % basil. The results showed that feeding basil to male lambs significantly increased testosterone concentration and decreased blood cortisol levels (P < 0.05). Additionally, feeding high levels of basil significantly improved sperm concentration, motility, and viability in the experimental samples, while reducing the level of complete abnormalities and malondialdehyde concentration (P < 0.05). The findings suggest that dietary supplementation of 25 % whole plant basil could be a useful strategy to improve sperm quality and increase testosterone secretion while reducing cortisol levels in male lambs.