Chamomile (Matricaria chamomilla L.) is a well-known medicinal plant species from the Asteraceae family often referred to as the “star among medicinal species.” Nowadays it is a highly favored and much used medicinal plant in folk and traditional medicine. Its multitherapeutic, cosmetic, and nutritional values have been established through years of traditional and scientific use and research. Chamomile has an established domestic (Indian) and international market, which is increasing day by day. The plant available in the market many a times is adulterated and substituted by close relatives of chamomile. This article briefly reviews the medicinal uses along with botany and cultivation techniques. Since chamomile is a rich source of natural products, details on chemical constituents of essential oil and plant parts as well as their pharmacological properties are included. Furthermore, particular emphasis is given to the biochemistry, biotechnology, market demand, and trade of the plant. This is an attempt to compile and document information on different aspects of chamomile and highlight the need for research and development.
Abstract Background Leaf morphology traits determine photosynthetic efficiency, high-density planting tolerance, and grain yield in maize (Zea mays L.). Understanding the genetic basis of these traits is of great practical significance for optimizing plant architecture and enhancing photosynthetic capacity to improve grain yield. Results In this study, six maternal and paternal testcrossing (TC/M and TC/P) populations were developed from recombinant inbred line (RIL) populations using three inbred lines—Y46 (tropical), Ye107 (temperate), and MON2 (subtropical)—in pairwise crosses. The inbreds represented the most representative lines from the three major heterotic groups: Suwan, Reid, and Non-Reid. Each RIL population was backcrossed to maternal and paternal parents, to generate TC/M and TC/P populations, respectively. All individuals from these testcross populations (TCM/Ps) were genotyped using genotyping-by-sequencing technology. Phenotyping for mid-parent heterosis of leaf morphology traits—leaf length (LL), leaf width (LW), and leaf angle (LA)—in these populations were evaluated over two years and across two field locations in China. The inclusive composite interval mapping (ICIM) approach was used to identify quantitative trait loci (QTLs) and candidate genes associated with heterosis for leaf morphological traits. The results showed that more QTLs for LL were identified in the Suwan × Non-Reid and Reid × Non-Reid heterotic groups than in Suwan × Reid heterotic groups. However, the largest QTL effects—with a phenotypic variance explained (PVE) of 23.31% for LL—were observed in the Reid × Non-Reid heterotic group. For LW and LA, more QTLs were detected in the Reid × Non-Reid and Suwan × Reid heterotic groups than in the Suwan x Non-Reid heterotic groups. The largest QTL effects were observed in the Reid × Non-Reid heterotic group, with a PVE of 32.32% for LW and 23.13% for LA. In total, seven stable QTLs and 14 candidate genes were identified. Through gene expression analysis, six candidate pleiotropic genes—the ones expressed in maize leaves—were identified, among which Zm00001d022618 and Zm00001d008625 play crucial roles in regulating heterosis formation of leaf morphological traits. Conclusion These findings provide insights into the complexity of heterosis determination in leaf morphological traits and may support precision breeding to optimize plant architecture and harness heterosis in maize.
Win Min Han, Bastian Neesgaard, Michael Knappik
et al.
Summary: Background: Data on cancer incidence and associated risk factors among women with HIV are limited. We investigated cancer burden among women with HIV. Methods: We included all women ≥18 years from the two large multicentre observational cohort collaborations (D:A:D and RESPOND). The primary outcomes were incidence of all cancers, HPV-related and common individual cancers including breast cancer, lung cancer, and non-Hodgkin lymphoma (NHL) from 2006 to 2021. Baseline was defined as the latest date of entry into local cohort enrolment and 1st January 2006 for D:A:D and 1st January 2012 for RESPOND. Participants were followed from baseline until the date of first cancer, final follow-up or administrative censoring—whichever occurred first. We assessed risk factors using multivariable Poisson regression by applying robust standard errors and determined a population attributable fraction (PAF) for key risk factors for cancers. Findings: Among 17,512 women included, median age at baseline was 39.5 years (interquartile range, IQR 32.5–46.0). Over 141,404 person-years (PYS) and a median 9.2 (5.5–10.1) years of follow-up, 832 women were diagnosed with any cancer; incidence rate 5.9 (95% CI 5.5–6.4)/1000 PYS, 163 HPV-related cancers (1.1 [1.0–1.3]/1000 PYS), 150 breast cancers (1.1 [0.9–1.2]/1000 PYS), 94 lung cancers (0.7 [0.5–0.8]/1000 PYS) and 72 NHL (0.5 [0.4–0.6]/1000 PYS). Older age (≥45 vs. <45 years), Southern Europe (vs. Western Europe) and smoking were associated with an increased risk of overall cancers. Lower pre-ART nadir CD4, time-updated CD4, and a prior AIDS diagnosis were associated with lung- and HPV-related cancer. In PAF analysis, smoking and HIV-related factors such as lower current CD4, nadir CD4 and HIV viremia significantly contributed to cancer risk. Interpretation: Our findings suggest that women with HIV older than 45 years, past or current immunosuppressed or current smokers could be candidates for intensified cancer screening and prevention. Funding: The Highly Active Antiretroviral Therapy Oversight Committee, The CHU St Pierre Brussels HIV Cohort, The Austrian HIV Cohort Study, The Australian HIV Observational Database, The AIDS Therapy Evaluation in the Netherlands national observational HIV cohort, The Brighton HIV Cohort, The National Croatian HIV Cohort, The EuroSIDA cohort, The Frankfurt HIV Cohort Study, The Georgian National AIDS Health Information System, The Nice HIV Cohort, The Isabel Foundation, The Modena HIV Cohort, The PISCIS Cohort Study, The Swiss HIV Cohort Study, The Swedish InfCare HIV Cohort, The Royal Free HIV Cohort Study, The San Raffaele Scientific Institute, The University Hospital Bonn HIV Cohort, The University of Cologne HIV Cohort, Merck Life Sciences, ViiV Healthcare, and Gilead Sciences.
Industrial effluents and wastewater containing elevated levels of heavy metals pose significant threats to water quality and groundwater, potentially leading to severe health risks.The physicochemical parameters and concentrations of heavy metals, including Cadmium (Cd), Chromium (Cr), Lead (Pb), Nickel (Ni), and Zinc (Zn), along with pollution indices of well water,were analyzed using standard procedures. The water samples exhibited alkaline properties and high electrical conductivity withheavy metal concentrations of 0.47 ± 0.07 mg/Lfor Cd, 0.038 ± 0.01 mg/Lfor Pb, 0.10 ± 0.02 mg/Lfor Ni, and 0.58 ± 0.05 mg/Lfor Zn, while Cr was below detection limits. The average Geoaccumulation index values for Cd, Ni, and Pb were 2.657, 1.763, and 1.287, respectively, whereas the Heavy Metal Pollution index exceeded 100 for all samples. The contamination factor values followed the order of Cd > Ni > Pb > Zn, and all samples had a Pollution Load Index above 1. The results indicated that Cd is a moderately strong pollutant, whereas Ni and Pb are moderate pollutants. This study provides a crucial baseline for assessing the migration of heavy metals from well water to vegetables, and ultimately to humans.
The genus Hatitia Brescovit, 1997 includes six species, the type species Hatitia yhuaia Brescovit, 1997, distributed in Peru, H. canchaque Brescovit, 1997, from Peru and Ecuador and H. defolonguei (Berland, 1913), H. perrieri (Berland, 1913) and H. riveti (Berland, 1913) all from Ecuador and H. sericiea (L. Koch, 1866) recorded in Colombia. The analysis of recent material from Hatitia specimens has allowed the records of Hatitia yhuaia to be expanded and to describe five additional species: H. zarate sp. nov. from Peru; H. winayhuayna sp. nov. from Peru and Bolivia; H. cajuata sp. nov. from Bolivia; H. oxapampa sp. nov. and H. machiguenga sp. nov. known only from Peru. Furthermore, Anyphaena mollicoma Keyserling, 1879 is considered a junior synonym of Hatitia sericea. As a result, a new emended diagnosis of the genus is presented. Additionally, complementary morphological data of Hatitia yhuaia are documented.
The effects of red and blue light on growth and steady-state photosynthesis have been widely studied, but there are few studies focusing on dynamic photosynthesis and the effects of LED pre-treatment on cucumber seedlings’ growth, so in this study, cucumber (<i>Cucumis sativus</i> L. cv. Jinyou 365) was chosen as the test material. White light (W), monochromatic red light (R), monochromatic blue light (B), and mixed red and blue lights with different red-to-blue ratios (9:1, 7:3, 5:5, 3:7, and 1:9) were set to explore the effects of red and blue light on cucumber seedlings’ growth, steady-state photosynthesis, dynamic photosynthesis, and subsequent growth under fluctuating light. The results showed that compared with R and B, mixed red and blue light was more suitable for cucumber seedlings’ growth, and the increased blue light ratios would decrease the biomass of cucumber seedlings under mixed red and blue light; cucumber seedlings under 90% red and 10% blue mixed light (9R1B) grew better than other treatments. For steady-state photosynthesis, blue light decreased the actual net photosynthetic rate but increased the maximum photosynthetic capacity by promoting stomatal development and opening; 9R1B exhibited higher actual net photosynthetic rate, but the maximum photosynthetic capacity was low. For dynamic photosynthesis, the induction rate of photosynthetic rate and stomatal conductance were also accelerated by blue light. For subsequent growth under fluctuating light, higher maximum photosynthetic capacity and photoinduction rate could not promote the growth of cucumber seedlings under subsequent fluctuating light, while seedlings pre-treated with 9R1B and B grew better under subsequent fluctuating light due to the high plant height and leaf area. Overall, cucumber seedlings treated with 9R1B exhibited the highest biomass and it grew better under subsequent fluctuating light due to the higher actual net photosynthetic rate, plant height, and leaf area.
The study aim was to optimise the C/N ratio, improve the compost quality, reduce pathogenic bacteria load in the compost, and improve guava yield. Vegetable wastes were mixed with cow dung, grasses, and food wastes in ratios of 4:3:2:1 (w/w) for achieving a C/N ratio of approximately 37. Co-composting is an important strategy because the mixture of bulking agents can help achieve optimal composting conditions. Experimental results were obtained from a pilot-scale rotary drum reactor with forced aeration. In the reactor, the temperature increased during the thermophilic phase (58±2 °C) and decreased after 10 days (54±2 °C). The pH values moderately increased, then decreased, and were near to neutral after maturation. The results indicated that co-composting of bio-wastes at a C/N ratio of 37.6%±1.02% could be effectively decomposed to reduce the residuals to just 13.6%±1.05% after 28 days. The microbial population increased in both mesophilic and thermophilic stages and decreased at the end of the composting, reflecting stability. The stable compost was applied to the growth of guava plant, and the yield was calculated. The organic compost improved plant growth, fruit yield, and enriched phytochemical compounds in the fruit and peels. The phytochemical compounds improved antioxidant activity in the guava fruits.
Background. The species Triticum petropavlovskyi Udacz. et Migusch. has a number of positive traits, but has rarely been used in breeding programs to improve bread wheat cultivars. The introgression of genetic material from this species into the Triticum aestivum L. gene pool will not only expand the genetic diversity of bread wheat with a set of traits valuable for breeders, but also help to analyze the expression of genes from T. petropavlovskyi in a new genetic environment. Materials and methods. Spring bread wheat lines L163 and L164 produced with the participation of bread wheat cv. ‘Voevoda’ and an accession of T. petropavlovskyi were target materials of the study. Conventional methods were applied to perform phenological, phytopathological, genetic, and bread quality evaluations. Statistical processing of the resulting data was carried out using the Agros-2.10 software. Results. L163 and L164 were studied for their morphological and phenological indicators, resistance to pathogens, productivity, and grain quality. Differences in a number of traits between the lines and the recipient cultivar were observed. The introgression of the T. petropavlovskyi genetic material into bread wheat showed both positive and negative effects on some agronomic characteristics. L163 was identified for its resistance to the pathogens of leaf rust and powdery mildew, and tolerance to cereal aphids. It combined high productivity with an increased grain protein content compared to the recipient cultivar. In addition, this line demonstrated good rheological properties of its dough. Conclusion. Merging genetic materials from T. petropavlovskyi and bread wheat cv. ‘Voevoda’ made it possible to produce lines with a set of positive characteristics. The selected line, L163, combined effective resistance to a number of diseases, high grain productivity, and good breadmaking qualities, so it was included in the breeding process.
Grzegorz J. Wolski, Mikołaj Latoszewski, D. Christine Cargill
et al.
A re-examination of the original collection of Plagiothecium novae-seelandiae described by Brotherus in 1916 indicated that this material is not homogeneous. Re-examination of the diagnosis of this species and morphological analysis supports that two separate taxa should be distinguished – Plagiothecium novae-seelandiae var. novae-seelandiae and P. novae-seelandiae var. brotheri var. nov. Also, comparisons with the original collection of Hypnum lamprostachys (= P. lamprostachys) showed differences, which supported their treatment as separate taxa. Revision of the genus Plagiothecium from Australasia (CANB, CHR, HO, MEL, WELT) and types of other species described from this part of the world (P. funale and P. lucidum) supported by the study of their diagnoses, qualitative and quantitative characteristics as well as mathematical analyses (PCA, HCA) allowed the division of the examined material into six separate groups – six separate taxa. Thereby, three distinct taxa are proposed – P. cordatum sp. nov., P. semimortuum sp. nov., and P. semimortuum var. macquariense var. nov. All taxa mentioned above are described in detail, their current known distribution and ecological preferences are also included. In addition, images illustrating their most important taxonomic features, as well as an original key to distinguish individual taxa are presented.
Tooba Mahboob, Polrat Wilairatana, Mohammed Rahmatullah
et al.
Background: Propolis is a natural resinous mixture produced by bees. It provides beneficial effects on human health in the treatment/management of many diseases. The present study was performed to demonstrate the anti-Acanthamoeba activity of ethanolic extracts of Propolis samples from Iran. The interactions of the compounds and essential proteins of Acanthamoeba were also visualized through docking simulation. Methods: The minimal inhibitory concentrations (MICs) of Propolis extract against Acanthamoeba trophozoites and cysts was determined in vitro. In addition, two-fold dilutions of each of agents were tested for encystment, excystment and adhesion inhibitions. Three major compounds of Propolis extract such as chrysin, tectochrysin and pinocembrin have been selected in molecular docking approach to predict the compounds that might be responsible for encystment, excystment and adhesion inhibitions of A. castellanii. Furthermore, to confirm the docking results, molecular dynamics (MD) simulations were also carried out for the most promising two ligand-pocket complexes from docking studies. Results: The minimal inhibitory concentrations (MICs) 62.5 and 125 µg/mL of the most active Propolis extract were assessed in trophozoites stage of Acanthamoeba castellanii ATCC30010 and ATCC50739, respectively. At concentrations lower than their MICs values (1/16 MIC), Propolis extract revealed inhibition of encystation. However, at 1/2 MIC, it showed a potential inhibition of excystation and anti-adhesion. The molecular docking and dynamic simulation revealed the potential capability of Pinocembrin to form hydrogen bonds with A. castellanii Sir2 family protein (AcSir2), an encystation protein of high relevance for this process in Acanthamoeba. Conclusions: The results provided a candidate for the development of therapeutic drugs against Acanthamoeba infection. In vivo experiments and clinical trials are necessary to support this claim.
The genus Apiospora includes endophytes, pathogens and saprobes, with a wide host range and geographic distribution. In this paper, six Apiospora strains isolated from diseased and healthy tissues of bamboo leaves from Hainan and Shandong provinces in China were classified using a multi-locus phylogeny based on a combined dataset of ITS, LSU, tef1 and tub2, in conjunction with morphological characters, host association and ecological distribution. Two new species, Apiospora dongyingensis and A. hainanensis, and a new record of A. pseudosinensis in China, are described based on their distinct phylogenetic relationships and morphological analyses. Illustrations and descriptions of the three taxa are provided, along with comparisons with closely related taxa in the genus.
Madeleine Héger, Pierre Noiset, Kiatoko Nkoba
et al.
Abstract Background Stingless bee honey (SBH) is a natural remedy and therapeutic agent traditionally used by local communities across the (sub-)tropics. Forest SBH represents a prime non-timber forest product (NTFP) with a potential to revitalize indigenous foodways and to generate income in rural areas, yet it is also used in a variety of non-food contexts that are poorly documented in sub-Saharan Africa and that collectively represent a significant part of the local traditional ecological knowledge (TEK) passed on across generations. Documenting TEK of local communities in African tropical forests facing global change is a pressing issue to recognize the value of their insights, to evaluate their sustainability, to determine how they contribute to enhancing conservation efforts, and how TEK generally contributes to the well-being of both the natural environment and the communities that rely on it. This is particularly important to achieve in Kenya’s only tropical rainforest at Kakamega where SBH production and non-food uses have evolved and diversified to a remarkable extent. Methods We used ethnographic techniques and methods, including semi-structured questionnaires and recorded interviews. We used snowball sampling, a non-probability sampling method where new interviewees were recruited by other respondents, to collectively form a sample consisting of 36 interviewees (including only one woman). Results Our results indicate that local communities in Kakamega were able to discriminate between six different and scientifically recognized stingless bee species, and they provided detailed accounts on the species-specific non-food uses of these SBH. Collectively, we recorded an array of 26 different non-food uses that are all passed on orally across generations in the Kakamega community. Conclusion Our results uncover the vast and hitherto unexpected diversity of TEK associated with SBH and pave the way for a systematic survey of SBH and their non-food uses across a network of communities in different environments and with different cultural backgrounds in the Afrotropics. This, along with parallel and more in-depth investigations into honey chemistry, will help develop a comprehensive understanding of SBH, offering insights into holistic ecosystem management, resilience and adaptation while in the mid- to long-term promoting cross-cultural exchanges and pathways for the revitalization of cultural practices and traditions.
I. D. Fadeeva, I. Yu. Ignatieva, A. G. Khakimova
et al.
Background. Development of cultivars with high stable yields and high grain quality is the main trend in wheat breeding. The aim of this study was to characterize a set of winter bread wheat accessions from the VIR collection and the working collection of Kazan Scientific Center in terms of their yield, protein content in grain (P, %), and swelling of flour in acetic acid (S, ml), and select the best accessions for the combination of these characters for use in a crossbreeding program.Materials and methods. Twenty-three winter bread wheat accessions were studied for the abovementioned characters in the north of the Middle Volga Region using conventional techniques. The study lasted three years (2016–2019).Results and conclusion. The yield of the accessions varied across the years of studies; however, none of them surpassed the reference cv. ‘Kazanskaya 560’. The values of protein content in grain were medium or high. The following accessions had high and stable levels of protein content in grain (15.1–16.1%): ‘TAW 42971/80’ (k-58363, Germany); ‘Lutescens 471 N8’ (Kazakhstan); ‘Rita’ (k-58057), ‘Scotty’ (k-59322) and ‘Nelson’ (all from the U.S.); ‘Moskovskaya 39’ (k-65160, Russia); ‘Bilotserkivchanka’ (k-64330) and ‘Barkan’ (k-64495) (both from Ukraine). Flour swelling power in acetic acid did not fall below 50 ml, attesting to the formation of high-quality grain. This was also confirmed by the protein quality index determined by the S : P ratio, which ranged from 3.6 to 4.7. Sources with high-quality protein were selected from the tested accessions for use in breeding: ‘CDC Clair’ (k-64168, Canada), ‘Lutescens 471 Н8’ (Kazakhstan), ‘Moskovskaya 39’ (Russia), ‘Barkan’ (Ukraine), and ‘Favorytka’ (k-64337, Ukraine).
Curcumin is the primary polyphenol in turmeric’s curcuminoid class. It has a wide range of therapeutic applications, such as anti-inflammatory, antioxidant, antidiabetic, hepatoprotective, antibacterial, and anticancer effects against various cancers, but has poor solubility and low bioavailability. Objective: To improve curcumin’s bioavailability, plasma concentration, and cellular permeability processes. The nanocurcumin approach over curcumin has been proven appropriate for encapsulating or loading curcumin (nanocurcumin) to increase its therapeutic potential. Conclusion: Though incorporating curcumin into nanocurcumin form may be a viable method for overcoming its intrinsic limitations, and there are reasonable concerns regarding its toxicological safety once it enters biological pathways. This review article mainly highlights the therapeutic benefits of nanocurcumin over curcumin.
Juan José Calixto Muñoz, Dora Luz Pinzón-Martínez, Jesús Castillón Jardón
et al.
Las harinas de grano entero de trigo (HGET) son fuente de fibra, antioxidantes, vitaminas, fitoquímicos y nutrientes y podrían suplir la creciente demanda de la industria panadera de trigos con alta calidad. El objetivo del estudio fue evaluar la calidad panadera de HGET de las variedades Cal Blanco F2011 (HCB); Mattchet F2011 (HM) y RSM-Norman F2008 (HN) cultivadas en México en otoño-inverno 2014 a 2016, mediante pruebas convencionales y una no convencional (consiste en la medición del volumen de fermentación de cada muestra de harina). Se realizaron determinaciones bromatológicas, reológicas, de calidad panadera por triplicado. Se realizó un ANDEVA, prueba por DMS (p<0,05) y un análisis de correlación. La HCB tiene mayor proteína (14,33%) que las testigos. Todas las harinas tienen tiempos de amasado favorables para buen volumen de pan, con buena fermentación y extensibilidad. Por lo anterior, las tres HGET experimentales resultaron ser competitivas a las harinas comerciales, pero HCB fue superior en casi todas las pruebas. La HM y HN, son similares a la HI en VOP, VOE y HAL volviéndolas comparables en calidad panadera. El pHf de HM y HCB es igual, pero en panificación la última es de mejor calidad haciéndola superior a las harinas integrales evaluadas. Se recomienda estudiar más la prueba de fermentación por la correlación con factores importantes en la diversas pruebas de calidad, esto con el fin de identificar si es factible o no su uso al evaluar la calidad de las harinas.
R. A. Abdullaev, O. V. Yakovleva, I. A. Kosareva
et al.
Background. Analyzing the adaptive potential of cultivated barley with the aim of using new genotypes selected and developed in the process of work is a priority trend in scientific research. The Ethiopian barleys adapted to a variety of soil and climate conditions are characterized by many valuable biological and agronomic traits. Of particular value are genotypes that combine resistance to harmful organisms and environmental stressors.Materials and methods. The collection accessions of Ethiopian barley possessing powdery mildew resistance were studied for tolerance to adverse edaphic factors (chloride salinity and high content of toxic aluminum ions in the soil). Resistance to abiotic stressors was assessed in laboratory experiments. In the study of salt tolerance, a ‘roll-based’ assessment technique was used, which takes into account the inhibition of root growth under salt (NaCl) stress conditions, compared with the reference conditions without salinization. Aluminum sensitivity of barley accessions was diagnosed in the early stages of plant development using the root test.Results and conclusions. A polymorphism of Ethiopian barleys for resistance to adverse edaphic factors was revealed. Twentyone new sources of barley resistance to toxic aluminum ions were identified, of which accessions k-8552 and k-22933 were classified as highly resistant. Accessions k-17554, k-19975, k-20029, k-20048, k-22752, k-23450 and k-25009 proved resistant to soil salinization. Barley accessions k-17554, k-22752 and k-25009 were characterized by complex resistance to powdery mildew, toxic aluminum ions, and chloride soil salinity.
Athipat Ngernmuen, Worrawit Suktrakul, Sureeporn Kate-Ngam
et al.
Jao Hom Nin (JHN) is a Thai rice variety with broad-spectrum resistant against rice blast fungus. JHN contains two rice blast resistant genes, <i>Pish</i> and <i>Pik</i>, located on chromosome 1 and on chromosome 11, respectively. To understand the blast resistance in JHN, the study of the defense mechanism related to the <i>Pish</i> and <i>Pik</i> genes is crucial. This study aimed to dissect defense response genes between the <i>Pish</i> and <i>Pik</i> genes using the RNA-seq technique. Differentially expressed genes (DEGs) of <i>Pish</i> and <i>Pik</i> backcross inbred lines were identified between 0 and 24 h after inoculation with rice blast spore suspension. The results showed that 1248 and 858 DEGs were unique to the <i>Pish</i> and <i>Pik</i> lines, respectively. The wall-associated kinase gene was unique to the <i>Pish</i> line and the zinc-finger-containing protein gene was unique to the <i>Pik</i> line. Pathogenicity-related proteins PR-4 and PR-10 were commonly found in both <i>Pish</i> and <i>Pik</i> lines. Moreover, DEGs functionally categorized in brassinosteriod, jasmonic acid, and salicylic acid pathways were detected in both <i>Pish</i> and <i>Pik</i> lines. These unique and shared genes in the <i>Pish</i> and <i>Pik</i> rice blast defense responses will help to dissect the mechanisms of plant defense and facilitate rice blast breeding programs.
Naïla Nativel, Elise Buisson, Fernando Augusto Oliveira Silveira
ABSTRACTWe studied the effects of seed storage on germination and dormancy alleviation in three species of Fabaceae endemic to campo rupestrein southeastern Brazil. Fresh seeds of Collaea cipoensis, Mimosa maguirei and Mimosa foliolosawere set to germinate and germination of seeds after four, five and 13 years of storage was tested. Seed viability was maintained for all species after the full storage period. Seed storage significantly increased germination percentage and decreased germination time for C. cipoensisand M. foliolosa, suggesting the alleviation of physical dormancy with storage. However, we did not find evidence of dormancy alleviation in M. maguirei since stored seeds showed a decrease in germination in comparison to that of fresh seeds. Our data indicate species-specific storage-mediated dormancy alleviation, which will have important implications for restoration of campo rupestre.