Hasil untuk "physics.acc-ph"

Xiaoxin Zou, Xiaoxi Huang, A. Goswami et al.

Niels Holten-Andersen, Matthew J. Harrington, H. Birkedal et al.

Ying-hong Guan, Jun Ma, Xu-chun Li et al.

Junyan Han, K. Burgess

Congcong Shen, J. Xiong, Huayong Zhang et al.

J. Rousk, P. Brookes, E. Bååth

I. Parolini, C. Federici, C. Raggi et al.

Exosomes secreted by normal and cancer cells carry and deliver a variety of molecules. To date, mechanisms referring to tumor exosome trafficking, including release and cell-cell transmission, have not been described. To gain insight into this, exosomes purified from metastatic melanoma cell medium were labeled with a lipid fluorescent probe, R18, and analyzed by spectrofluorometry and confocal microscopy. A low pH condition is a hallmark of tumor malignancy, potentially influencing exosome release and uptake by cancer cells. Using different pH conditions as a modifier of exosome traffic, we showed (i) an increased exosome release and uptake at low pH when compared with a buffered condition and (ii) exosome uptake by melanoma cells occurred by fusion. Membrane biophysical analysis, such as fluidity and lipid composition, indicated a high rigidity and sphingomyelin/ganglioside GM3 (N-acetylneuraminylgalactosylglucosylceramide) content in exosomes released at low pH. This was likely responsible for the increased fusion efficiency. Consistent with these results, pretreatment with proton pump inhibitors led to an inhibition of exosome uptake by melanoma cells. Fusion efficiency of tumor exosomes resulted in being higher in cells of metastatic origin than in those derived from primary tumors or normal cells. Furthermore, we found that caveolin-1, a protein involved in melanoma progression, is highly delivered through exosomes released in an acidic condition. The results of our study provide the evidence that exosomes may be used as a delivery system for paracrine diffusion of tumor malignancy, in turn supporting the importance of both exosomes and tumor pH as key targets for future anti-cancer strategies.

G. Nicol, S. Leininger, C. Schleper et al.

Y. Al-Degs, M. El‐Barghouthi, A. El‐Sheikh et al.

K. Caldeira, M. Wickett, P. Duffy et al.

P. Gupta, K. Vermani, S. Garg

G. Helmlinger, F. Yuan, M. Dellian et al.

Jun Yin, Ying Hu, Juyoung Yoon

Volkan Yesilyurt, M. Webber, Eric A. Appel et al.

Chuanxi Wang, zhenzhu xu, Hao Cheng et al.

M. Koziolek, M. Grimm, D. Becker et al.

Christopher C. Deng, William L. A. Brooks, K. Abboud et al.

W. Guo, Chun-hua Lu, R. Orbach et al.

Špela Zupančič, Z. Lavrič, J. Kristl

Katherine M. Strickler, A. Fremier, C. Goldberg