Hasil untuk "Pharmacy and materia medica"

Rony Abdi Syahputra, Asriadi, Arnika Gloria Br Sitorus et al.

MicroRNAs are essential post-transcriptional regulators of gene expression, playing dual roles in oncogenesis as either oncogenes or tumour suppressors. Aberrant miRNA expression is a critical factor in cancer progression, influencing tumour development, metastasis, resistance to apoptosis, and therapy evasion. Concurrently, therapeutic peptides have emerged as promising modulators of miRNA activity due to their specificity, low toxicity, and ability to interact with nucleic acids. This review provides of peptide-mediated modulation of miRNA pathways in cancer, emphasising strategies such as degradation of oncogenic miRNAs, stabilization of tumour-suppressive miRNAs, and the targeted delivery of miRNA therapeutics. These methodologies demonstrate the potential to reverse malignant phenotypes by reducing cell proliferation, suppressing metastasis, restoring normal cellular functions, and promoting apoptosis. The integration of peptide-based delivery systems with miRNA-targeted therapies represents a novel paradigm in precision oncology, addressing key challenges related to therapeutic specificity and systemic delivery.

Nuria Senar, Aeilko H. Zwinderman, Michel H. Hof and

Canonical Correlation Analysis, CCA, is a widely used multivariate method in omics research for integrating high dimensional datasets. CCA identifies hidden links by deriving linear projections of features maximally correlating datasets. For standard CCA, observations must be independent of each other. As a result, it cannot properly deal with repeated measurements. Current CCA extensions dealing with these challenges either perform CCA on summarized data or estimate correlations for each measurement. While these techniques factor in the correlation between measurements, they are sub-optimal for high dimensional analysis and exploiting this datas longitudinal qualities. We propose a novel extension of sparse CCA that incorporates time dynamics at the latent level through longitudinal models. This approach addresses the correlation of repeated measurements while drawing latent paths, focusing on dynamics in the correlation structures. To aid interpretability and computational efficiency, we implement a penalty to enforce fixed sparsity levels. We estimate these trajectories fitting longitudinal models to the low dimensional latent variables, leveraging the clustered structure of high dimensional datasets, thus exploring shared longitudinal latent mechanisms. Furthermore, modeling time in the latent space significantly reduces computational burden. We validate our models performance using simulated data and show its real world applicability with data from the Human Microbiome Project. Our CCA method for repeated measurements enables efficient estimation of canonical correlations across measurements for clustered data. Compared to existing methods, ours substantially reduces computational time in high dimensional analyses as well as provides longitudinal trajectories that yield interpretable and insightful results.

Patonah Hasimun, Hendra Mahakam Putra, Fitriani Choerunnisa

A strong link exists between arterial stiffening and cardiovascular conditions, particularly hypertension. Hypertension, a significant contributor to mortality rates, particularly affects elderly populations. The processed form of Allium sativum, known as black garlic, is renowned for its medicinal properties, including its ability to lower blood pressure. This study examined the blood pressure-lowering effects of black garlic extract, employing male Wistar rats aged two to three months.The experiment involved six distinct rat groups, each comprising of four animals. The two groups served as controls, whereas the remaining four underwent different treatments. Captopril was administered to one group at a dose of 2.5 mg/kg, whilst the remaining three groups were given black garlic extract as BG50, BG100, and BG200. All groups, with the exception of the control normal, underwent a 28-day induction protocol that included 25% fructose in drinking water, cholesterol (200 mg/kg), and propylthiouracil (12.5 mg/kg). The test substances were administered starting on day 14 concurrently with the induction phase. The evaluated parameters included blood pressure, arterial stiffness, nitric oxide (NO) levels, and renal histopathology. Black garlic extract significantly lowered blood pressure, enhanced arterial elasticity, and increased NO levels (p < 0.05) compared with the controls. Renal histopathological analysis revealed protective effects, suggesting prevention of kidney damage. These findings suggest that black garlic may have cardiorenal protective effects and could be useful for managing hypertension and arterial stiffness.

Shruti Vishwakarma, Gautam Kumar, Ritika Jain et al.

Background: Chronic alcohol consumption does not just undermine physical health it also quietly chips away at closeness and emotional connection. One of its most ignored results is sexual dysfunction. This study looked at how male sexual function is affected by alcohol consumption and how its severity affects the degree of impairment. Methods: A cross-sectional study of 162 alcohol-dependent men and 50 age-matched controls was conducted at the psychiatry OPD of a tertiary care hospital. Administered were validated instruments Alcohol Use Disorders Identification Test (AUDIT) for alcohol consumption severity, Premature Ejaculation Diagnostic Tool (PEDT) for ejaculation and International Index of Erectile Function (IIEF) for erectile and sexual domains. Results: Nearly 8 of 10 alcohol-dependent men (77.2%) reported some sort of sexual dysfunction, compared with 44.0% in controls (P < 0.001). Of those, erectile dysfunction (63.0% vs. 18.0%) was the most prevalent, followed by decreased sexual desire (57.4% vs. 28.0%) and early ejaculation (46.3% vs. 28.0%). Significantly, more AUDIT scores were related to more dysfunction in all sexual domains. Conclusion: More drinking, less intimacy the link is obvious. Acknowledging and handling sexual health in these people could help not just their relationships but also their road to recovery.

Lorenzo Di Sarno, Serena Ferretti, Lavinia Capossela et al.

<b>Background:</b> Traumatic brain injury (TBI) and hypoxic–ischemic encephalopathy (HIE) are major causes of long-term neurological disability in children, with limited options for effective neuronal recovery. Recent research has highlighted the therapeutic potential of nerve growth factor (NGF) in promoting neural repair through mechanisms such as neuroprotection, neurogenesis, and the modulation of neuroinflammation. This review evaluates the current evidence on NGF as a treatment strategy for pediatric brain injury, emphasizing its mechanisms of action and translational clinical applications. <b>Methods:</b> A comprehensive review was conducted using the PubMed, Scopus, and Cochrane CENTRAL databases to identify studies published between 1 January 1978 and 1 March 2025, investigating NGF in the context of brain injury. The inclusion criteria comprised studies assessing neurological outcomes through clinical scales, biochemical markers, neuroimaging, or electrophysiological examinations. <b>Results:</b> Seventeen studies met the inclusion criteria, encompassing both preclinical and clinical research. Preclinical models consistently demonstrated that NGF administration reduces neuroinflammation, enhances neurogenesis, and supports neuronal survival following TBI and HIE. Clinical studies, including case reports of pediatric patients treated with intranasal NGF, reported improvements in motor and cognitive function, neuroimaging findings, and electrophysiological parameters, with no significant adverse effects observed. <b>Conclusions:</b> NGF demonstrates significant promise as a neuroprotective and neuroregenerative agent in pediatric brain injury, with both experimental and early clinical evidence supporting its safety and efficacy. Large-scale controlled clinical trials are warranted to validate these preliminary findings and to determine the optimal dosage regimens and administration schedules for NGF in the treatment of TBI and HIE.

Bhavika A. Bhavsar, Vaishali Jain, Annu Kumari et al.

Objective: To Evaluate The Antimicrobial Properties of Double Antibiotic Paste and Morinda citrifolia And Propolis Paste Used In Regenerative Endodontics. Materials and Method: Using intact human teeth, radicular dentin samples were prepared (dimensions 4×4×2 mm) by following a standard method. The samples were cut using a diamond saw at minimal speed using water as coolant and smoothed with abrasive papers. To expose the dentin tubules and eliminate the smear layer, they were treated with NaOCl (1.5%), distilled water, and EDTA (17%). A total of 30 dentin samples went through a three-week anaerobic infection with bacterial biofilms sourced from the root canal of an underdeveloped tooth showing pulp necrosis. Group 1 acted as the control, while Group 2 received treatment with DAP, and Group 3 was treated with a paste of Morinda citrifolia and Propolis. After a week, pastes were cleaned, and specimens were dipped in phosphate-buffered saline for a day. The biofilm was released from each specimen by sonicating and vortexing it for 30 seconds. It was then diluted, spiral-plated on blood agar, and cultured anaerobically for a day. An automated colony counter was utilized to measure colony-forming units (CFU/mL), and statistical analysis was conducted using the Wilcoxon rank-sum test. Result: The wash from dentin samples treated with DAP and MCP showed minimal CFU counts, indicating both pastes’ high efficiency against biofilm-forming microbes from the canals of an immature tooth exhibiting pulp necrosis. Conclusion: Dentin samples infused with DAP and MCP showed minimal CFU counts, indicating their high effectiveness against biofilm-forming microbes from the root canal of an underdeveloped tooth with pulp necrosis. Additional research is required to validate these findings.

Salvador Nadal Domingo, Desamparados Domingo Tortajada

Mujer de 42 años acude a la farmacia comunitaria en busca de asesoramiento nutricional debido a un aumento de 19 kilogramos en los últimos 4 meses, además de ayuda para manejar sus problemas de ansiedad. Desde la FC ofrecemos ayuda nutricional específica mediante un régimen dietético personalizado y, además, le proponemos realizar una comparativa de la medicación que utiliza en la situación actual y con la que usaba previamente al aumento de peso. La paciente acepta y, efectivamente, observamos ciertos cambios que pueden estar provocando ese aumento de peso. Por ello, derivamos el caso al Médico de Atención Primaria aportando la información relativa a los cambios en la medicación, últimas pruebas médicas y otros indicadores que podrían contribuir al aumento de peso. Paralelamente, establecemos un seguimiento semanal para controlar sus hábitos dietéticos, además de su estilo de vida.

Shery Jacob, Fathima Sheik Kather, Sai H. S. Boddu et al.

Natural substances, especially those derived from plants, exhibit a diverse range of therapeutic benefits, such as antioxidant, anti-inflammatory, anticancer, and antimicrobial effects. Nevertheless, their use in clinical settings is frequently impeded by inadequate solubility, limited bioavailability, and instability. Nanovesicular carriers, such as liposomes, niosomes, ethosomes, transferosomes, transethosomes, and cubosomes, have emerged as innovative phytochemical delivery systems to address these limitations. This review highlights recent developments in vesicular nanocarriers for phytochemical delivery, emphasizing preparation techniques, composition, therapeutic applications, and the future potential of these systems. Phytosomes, along with their key advantages and various preparation techniques, are extensively described. Various in vitro and in vivo characterization techniques utilized for evaluating these nanovesicular carriers are summarized. Completed clinical trials and patents granted for nanovesicles encapsulating phytochemicals designed for systemic delivery are tabulated. Phytochemical delivery via vesicular carriers faces challenges such as low stability, limited active loading, scalability issues, and high production costs. Additionally, immune clearance and regulatory hurdles hinder clinical application, requiring improved carrier design and formulation techniques.

Vívian Terra de Azevedo Decúpero, Caroline Damascena Cardoso, Sarah Santos Gomes et al.

Introdução: As universidades públicas desempenham um papel fundamental na produção de conhecimento científico, e por meio da extensão universitária, conectam ensino e pesquisa às necessidades sociais. Nesse contexto, o curso de Farmácia do CCENS-UFES, em parceria com a escola EEEFM Sirena Rezende Fonseca, localizada no distrito de Celina (Alegre-ES), desenvolveu, com o apoio da FAPES, um projeto voltado para a capacitação e conscientização dos alunos do ensino médio sobre Infecções Sexualmente Transmissíveis (IST). A metodologia adotada integrou abordagens teóricas e práticas, com foco em ensaios imunodiagnósticos, permitindo aos estudantes uma aplicação real dos conhecimentos adquiridos. Um dos casos abordados durante o projeto envolveu a história fictícia de Gabriel, aluno do programa de iniciação científica Jr. Gabriel, ao aprender sobre as IST, foi capaz de reconhecer os sinais de uma possível infecção em seu irmão Henrique, que trabalha na roça. Henrique, ao notar uma lesão genital, procurou Gabriel em busca de ajuda. A partir do aprendizado sobre as IST, Gabriel suspeitou da infecção e sugeriu que seu irmão realizasse a bateria de testes rápidos no Centro de Testagem e Aconselhamento de Alegre. O resultado positivo para sífilis evidenciou como a disseminação do conhecimento no âmbito escolar pode ter um impacto direto na saúde e bem-estar da comunidade. Além dessa aplicação prática, observou-se que, ao longo da experiência, muitos alunos demonstraram desconhecimento sobre IST, incluindo sinais, sintomas, modos de transmissão e formas de tratamento. No entanto, houve grande receptividade ao aprendizado, refletida na participação ativa nas atividades laboratoriais e discussões. A evolução na compreensão e na aplicação dos conceitos foi uma das conquistas mais significativas do projeto. Apesar do entusiasmo gerado nas atividades práticas, um dos desafios foi manter o interesse dos alunos durante as exposições teóricas. Para lidar com isso, foram inseridos casos cotidianos, como o mencionado acima, estruturados com narrativas interativas, nas quais os alunos contribuíam com suas próprias soluções para as situações apresentadas. Essa abordagem contribuiu para uma maior imersão no tema e facilitou a assimilação do conteúdo. O alcance do projeto foi limitado pelo número reduzido de alunos atendidos, o que comprometeu sua abrangência. Diante dos resultados, é clara a necessidade de investimentos públicos para ampliar iniciativas como o PICJr, possibilitando a inclusão de mais estudantes e a exploração de outras questões de saúde. A integração entre escolas, universidades e serviços de saúde é fundamental para fortalecer a educação em saúde e incentivar o ingresso no ensino superior, promovendo impactos positivos na formação dos alunos e na comunidade.

Miguel F. Arevalo-Castiblanco, Yejin Wi, Marzia Cescon and et al.

This paper presents the application of a Distributed Model Reference Adaptive Control (DMRAC) strategy for robust multi-agent synchronization of a network of drones. The proposed approach enables the development of controllers capable of accommodating differences in real-life model parameters between agents, thereby enhancing overall network performance. We compare the performance of the adaptive control laws with classical PID controllers for the reference tracking task. Each follower drone has a model reference adaptive controller that continuously updates its parameters based on real-time feedback and reference model information. This adaptability ensures an adequate performance that, compared to conventional non-adaptive techniques, can reduce the amount of energy required and consequently increase the operating duration of the drones. The experimental results, particularly in vertical velocity control, underscore the effectiveness of the proposed approach in achieving synchronized behavior.

N. Heidari, A. A. Araújo Filho, V. Vertogradov and et al.

This work examines the implications of a black hole featuring a de Sitter core. We begin by analyzing the spacetime and event horizon in the presence of de Sitter core. Then the partial wave equation necessary for calculating quasinormal modes is derived and the relation of scalar quasinormal modes with the de Sitter core parameter is explored. Subsequently, we explore the greybody factors and their correspondence with the gravitational quasinormal modes. We also analyze the emission rate. Finally, variations in the thin accretion disks and the influence of de Sitter core spacetime on the optical appearance of the black hole are discussed as well.

M. Sharif, and Malick Sallah

In this study, we utilize the minimal geometric deformation technique of gravitational decoupling to extend the regular Bardeen black hole, leading to the derivation of new black hole solutions within the framework of Rastall theory. By decoupling the field equations associated with an extended matter source into two subsystems, we address the first subsystem using the metric components of the regular Bardeen black hole. The second subsystem, incorporating the effects of the additional source, is solved through a constraint imposed by a linear equation of state. By linearly combining the solutions of these subsystems, we obtain two extended models. We then explore the distinct physical properties of these models for specific values of the Rastall and decoupling parameters. Our investigations encompass effective thermodynamic variables such as density and anisotropic pressure, asymptotic flatness, energy conditions, and thermodynamic properties including Hawking temperature, entropy, and specific heat. The results reveal that both models violate asymptotic flatness of the resulting spacetimes. The violation of energy conditions indicate the presence of exotic matter, for both models. Nonetheless, the energy density, radial pressure, as well as the Hawking temperature exhibit acceptable behavior, while the specific heat and Hessian matrix suggest thermodynamic stability.

Mohammad Rehan Asad, Ritu Kumar Ahmad, Husam A. Almalki et al.

Background: This study aimed to demonstrate the association between smartphone use and De Quervain’s syndrome in Saudi Arabian teenagers, as well as to establish the length of phone use among these patients in order to evaluate whether it was connected to the emergence of De Quervain’s tenosynovitis. Methodology: This cross-sectional observational study was conducted among teenagers in Saudi Arabia studying in public and private schools. Most of the inquiries were closed-ended and sought information regarding the students’ use of various mobile phone sizes, regular text messaging, discomfort in the wrist or thumb, swelling or a snapping sound over the thumb, and limitations or pain aggravation when handling various objects. Results: The total number of teenagers that participated in the study was 200; 111 (55.5%) of them were males, while the remaining 89 (44.5%) were females. Out of 200 participants, 135 (67.5%) tested positive for Finkelstein disease; of these, 21 (15.5%) used smartphones for under 4 hours, 53 (39.2%) used smartphones for 5–7 hours, and 21 (15.5%) used smartphones more than 10 hours with P value of 0.008, which is significant and hence concluded that duration of using mobile phones had impact on De Quervain’s disease. Conclusion: In conclusion, Finkelstein’s sign, a marker that De Quervain’s illness is widespread, was present in 67.5% of the patients. Current findings suggest that De Quervain cannot be completely ruled out because of this population’s propensity to develop it.

Annagiulia Di Trana, Alessandro Di Giorgi, Giorgia Sprega et al.

In 2023, hexahydrocannabinol (HHC) attracted the attention of international agencies due to its rapid spread in the illegal market. Although it was discovered in 1940, less is known about the pharmacology of its two naturally occurring epimers, 9(R)-HHC and 9(S)-HHC. Thus, we aimed to investigate the disposition of hexahydrocannabinol epimers and their metabolites in whole blood, urine and oral fluid following a single controlled administration of a 50:50 mixture of 9(R)-HHC and 9(S)-HHC smoked with tobacco. To this end, six non-user volunteers smoked 25 mg of the HHC mixture in 500 mg of tobacco. Blood and oral fluid were sampled at different time points up to 3 h after the intake, while urine was collected between 0 and 2 h and between 2 and 6 h. The samples were analyzed with a validated HPLC-MS/MS method to quantify 9(R)-HHC, 9(S)-HHC and eight metabolites. 9(R)-HHC showed the highest C_max and AUC_0–3h in all the investigated matrices, with an average concentration 3-fold higher than that of 9(S)-HHC. In oral fluid, no metabolites were detected, while they were observed as glucuronides in urine and blood, but with different profiles. Indeed, 11nor-9(R)-HHC was the most abundant metabolite in blood, while 8(R)OH-9(R) HHC was the most prevalent in urine. Interestingly, 11nor 9(S) COOH HHC was detected only in blood, whereas 8(S)OH-9(S) HHC was detected only in urine.

Elżbieta Studzińska-Sroka, Magdalena Paczkowska-Walendowska, Zuzanna Woźna et al.

<i>Sambuci folium</i> (elderberry leaves) have been used in traditional medicine, mainly externally, to treat skin diseases and wounds. Therefore, the aim of this study was to screen the biological activity of elderberry leaves (antioxidant potential and possibility of inhibition of tyrosinase and hyaluronidase enzymes) combined with phytochemical analysis. For this purpose, a phytochemical analysis was carried out. Elderberry leaves of 12 varieties (“Sampo”, “Obelisk”, “Dwubarwny”, “Haschberg”, “Haschberg 1”, “Koralowy”, “Sambo”, “Black Beauty”, “Black Tower”, “Golden hybrid”, “Samyl”, “Samyl 1”) in two growth stages. The compounds from the selected groups, phenolic acids (chlorogenic acid) and flavonols (quercetin), were chromatographically determined in hydroalcoholic leaf extracts. All tested elderberry leaf extracts showed antioxidant effects, but the most promising potential: very high compounds content (TPC = 61.85 mg GAE/g), antioxidant (e.g., DPPH IC₅₀ = 1.88 mg/mL; CUPRAC IC_0.5 = 0.63 mg/mL) and optimal anti-inflammatory (inhibition of hyaluronidase activity 41.28%) activities were indicated for older leaves of the “Sampo” variety. Additionally, the extract obtained from “Sampo” and “Golden hybrid” variety facilitated the treatment of wounds in the scratch test. In summary, the best multidirectional pro-health effect in treating skin inflammation was specified for “Sampo” leaves II extract (leaves during the flowering period); however, wound treatment was noted as rich in chlorogenic acid younger leaf extracts of the “Golden hybrid” variety.

Yaoxun Xu, Baiji Liu, Qiaochu Huang and et al.

Due to the mismatch between the source and target domains, how to better utilize the biased word information to improve the performance of the automatic speech recognition model in the target domain becomes a hot research topic. Previous approaches either decode with a fixed external language model or introduce a sizeable biasing module, which leads to poor adaptability and slow inference. In this work, we propose CB-Conformer to improve biased word recognition by introducing the Contextual Biasing Module and the Self-Adaptive Language Model to vanilla Conformer. The Contextual Biasing Module combines audio fragments and contextual information, with only 0.2% model parameters of the original Conformer. The Self-Adaptive Language Model modifies the internal weights of biased words based on their recall and precision, resulting in a greater focus on biased words and more successful integration with the automatic speech recognition model than the standard fixed language model. In addition, we construct and release an open-source Mandarin biased-word dataset based on WenetSpeech. Experiments indicate that our proposed method brings a 15.34% character error rate reduction, a 14.13% biased word recall increase, and a 6.80% biased word F1-score increase compared with the base Conformer.

Qihang Ma, Kai Leong Chong, Feng Feng et al.

The high-order Target ENO (TENO) scheme, known for its innovative weighting strategy, has demonstrated strong potential for complex flow predictions. This study extends the TENO weighting approach to develop non-oscillatory central TENO (CTENO and CTENOZ) family schemes for unstructured meshes. The CTENO schemes employ compact directional stencils, which increase the likelihood of finding stencils within smooth regions. The design is intentionally compact to simplify the implementation of directional stencils. An effective scale separation strategy is adopted using an ENO-like stencil selection method, which employs large central stencils in smooth regions to achieve high-order accuracy, and smaller directional stencils near discontinuities to improve shock-capturing capabilities. Extensive tests involving CWENO, TENO, CTENO, and CTENOZ schemes were conducted to assess their performance in terms of accuracy, robustness, parallel scalability, and computational efficiency. The findings indicate that the proposed CTENO and CTENOZ schemes deliver high-order precision, lower numerical dissipation, and excellent shock-capturing performance.

Irina Subotić, and Dominic Groß

We analyze a dual-port grid-forming (GFM) control for power systems containing ac and dc transmission, converter-interfaced generation and energy storage, and legacy generation. To operate such a system and provide standard services, state-of-the-art control architectures i) require assigning grid-following (GFL) and GFM controls to different converters, and ii) result in highly complex system dynamics. In contrast, dual-port GFM control (i) subsumes common functions of GFM and GFL controls in a simple controller, ii) can be applied to a wide range of emerging technologies independently of the network configuration, and iii) significantly reduces system complexity. In this work, we provide i) an end-to-end modeling framework that allows to model complex topologies through composition of reduced-order device models, ii) an in-depth discussion of universal dual-port GFM control for emerging power systems, and iii) end-to-end stability conditions that cover a wide range of network topologies, emerging technologies, and legacy technologies. Finally, we validate our findings in detailed case studies.

Hossein Niknahad, Ali Nadgaran, Sepideh Alidaee et al.

Cholestasis is not only influences the hepatic function but also damages many other organs. Lung injury is a critical secondary organ damage associated with cholestasis/cirrhosis. Pulmonary histopathological alterations, respiratory distress, and hypoxia are related to cholestasis/cirrhosis-induced lung injury. It has been found that oxidative stress plays a crucial role in this complication. The current study was designed to investigate the effect of N-acetyl cysteine (NAC) and dithiothreitol (DTT) as thiol-reducing and antioxidant agents against cholestasis-induced lung injury. Bile duct ligated (BDL) rats were monitored for the presence of inflammatory cells, TNF-α, and IgG levels in their broncho-alveolar fluid (BALF) at scheduled time intervals (3, 7, 14, and 28 days post-BDL surgery). These markers reached their highest level in the BALF of BDL rats on day 28 after the surgery. Therefore, in another set of experiments, the BDL animals were treated with NAC (100 and 300 mg/kg/day, i.p, for 28 consecutive days) and DTT (10 and 20 mg/kg/day, i.p, for 28 consecutive days). Meanwhile, a significant increase in the levels of TNF-α and IgG was detected in the BALF of BDL rats. The BALF level of neutrophils, monocytes, and lymphocytes was also significantly increased in cholestatic animals. A significant increase in lung tissue biomarkers of oxidative stress was detected in the BDL rats. It was found that NAC and DTT could significantly blunt pulmonary damage induced by cholestasis. The effects of these agents on oxidative stress biomarkers and inflammatory response seem to play a pivotal role in their mechanisms of protective properties.

Gowri Rajkumar, Mihiri Rangika Jayasinghe, Vinotha Sanmugarajah

Introduction: The management of Diabetes mellitus is a major contest for clinicians in nowadays. Uncontrolled hyperglycaemia increases the risk of numerous complications in the body. Although a number of antidiabetic drugs are available for therapeutic intervention, herbal management for diabetes is encouraged due to its low side effects and effectiveness, throughout the world. This current in-vitro study is a comparative evaluation of antimicrobial activities of four selected anti-diabetic medicinal plant seeds namely Syzygium cumini (L.) Skeels, Sinapis alba L., Trigonella foenum-graecum L. and Nigella sativa L. which are commonly found in Jaffna District, Sri Lanka. Materials and method: Crude ethanol extract of seeds was investigated for their antibacterial activity against three bacterial strains as Enterococcus faecalis, Staphylococcus aureus and Escherichia coli by using agar well diffusion method in three replicates. The statistical analysis was carried out using one-way Analysis of variance. Results: The seeds extract of S. cumini showed maximum zone of inhibitions (24.70, 16.147, 10.37 mm) and S. alba represented minimum zone of inhibitions (1.08, 1.08, 0 mm) against all three types of human pathogens (E. faecalis, S. aureus and E. coli) respectively. Results showed that significant amount of inhibition zone was obtained against all the selected bacterial species which were comparable with positive control streptomycin. Conclusion: The chances of discovering antimicrobial activities in seeds of S.cumini extract is the highest and it is proposed that the plants which presented significant antimicrobial activities should be tested in detail to reveal their potential as probable antibiotics against particular pathogens. Therefore, this current research process demonstrates the S.cumini seeds can be used for developing nutraceutical products and long-term storage herbal drugs for the treatment of infectious diseases as well as diabetes in future.