Evaluate the Antimicrobial Properties of Double Antibiotic Paste (DAP) and Morinda Citrifolia and Propolis Paste (MCP) Used in Regenerative Endodontics: An In vitro Study

Objective: To Evaluate The Antimicrobial Properties of Double Antibiotic Paste and Morinda citrifolia And Propolis Paste Used In Regenerative Endodontics. Materials and Method: Using intact human teeth, radicular dentin samples were prepared (dimensions 4×4×2 mm) by following a standard method. The samples were cut using a diamond saw at minimal speed using water as coolant and smoothed with abrasive papers. To expose the dentin tubules and eliminate the smear layer, they were treated with NaOCl (1.5%), distilled water, and EDTA (17%). A total of 30 dentin samples went through a three-week anaerobic infection with bacterial biofilms sourced from the root canal of an underdeveloped tooth showing pulp necrosis. Group 1 acted as the control, while Group 2 received treatment with DAP, and Group 3 was treated with a paste of Morinda citrifolia and Propolis. After a week, pastes were cleaned, and specimens were dipped in phosphate-buffered saline for a day. The biofilm was released from each specimen by sonicating and vortexing it for 30 seconds. It was then diluted, spiral-plated on blood agar, and cultured anaerobically for a day. An automated colony counter was utilized to measure colony-forming units (CFU/mL), and statistical analysis was conducted using the Wilcoxon rank-sum test. Result: The wash from dentin samples treated with DAP and MCP showed minimal CFU counts, indicating both pastes’ high efficiency against biofilm-forming microbes from the canals of an immature tooth exhibiting pulp necrosis. Conclusion: Dentin samples infused with DAP and MCP showed minimal CFU counts, indicating their high effectiveness against biofilm-forming microbes from the root canal of an underdeveloped tooth with pulp necrosis. Additional research is required to validate these findings.