Hasil untuk "Veterinary medicine"

Sam T. Xiao, N. Dhand, Zhiyong Wang et al.

Deep learning (DL), a subfield of artificial intelligence (AI), involves the development of algorithms and models that simulate the problem-solving capabilities of the human mind. Sophisticated AI technology has garnered significant attention in recent years in the domain of veterinary medicine. This review provides a comprehensive overview of the research dedicated to leveraging DL for diagnostic purposes within veterinary medicine. Our systematic review approach followed PRISMA guidelines, focusing on the intersection of DL and veterinary medicine, and identified 422 relevant research articles. After exporting titles and abstracts for screening, we narrowed our selection to 39 primary research articles directly applying DL to animal disease detection or management, excluding non-primary research, reviews, and unrelated AI studies. Key findings from the current body of research highlight an increase in the utilisation of DL models across various diagnostic areas from 2013 to 2024, including radiography (33% of the studies), cytology (33%), health record analysis (8%), MRI (8%), environmental data analysis (5%), photo/video imaging (5%), and ultrasound (5%). Over the past decade, radiographic imaging has emerged as most impactful. Various studies have demonstrated notable success in the classification of primary thoracic lesions and cardiac disease from radiographs using DL models compared to specialist veterinarian benchmarks. Moreover, the technology has proven adept at recognising, counting, and classifying cell types in microscope slide images, demonstrating its versatility across different veterinary diagnostic modality. While deep learning shows promise in veterinary diagnostics, several challenges remain. These challenges range from the need for large and diverse datasets, the potential for interpretability issues and the importance of consulting with experts throughout model development to ensure validity. A thorough understanding of these considerations for the design and implementation of DL in veterinary medicine is imperative for driving future research and development efforts in the field. In addition, the potential future impacts of DL on veterinary diagnostics are discussed to explore avenues for further refinement and expansion of DL applications in veterinary medicine, ultimately contributing to increased standards of care and improved health outcomes for animals as this technology continues to evolve.

Hanan Hendawy, Ahmed Farag, Asmaa Elhaieg et al.

Effective bladder reconstruction remains a significant challenge in urology, particularly for conditions requiring partial or complete bladder replacement. In this study, the efficacy is evaluated of two types of scaffolds, silk fibroin (SF) and adipose-derived stem cells (ADSCs-SF), in promoting bladder regeneration and their associated outcomes. A rat model was used to compare the surgical outcomes and morphological recovery of bladder tissues implanted with SF and ADSCs-SF scaffolds. Post-operative recovery, including voiding ability and complication rates, was assessed. The morphological and histological changes of the regenerated bladder tissue were evaluated at multiple time points (2, 4, 8, and 12 weeks) using gross tissue analysis, histometric assessments, and immunohistochemical staining. Both scaffold types demonstrated successful integration into the bladder wall with no significant differences in body weight or voiding issues. The SF scaffold group exhibited graft shrinkage and a 41.6% incidence of bladder calculus formation. In contrast, the ADSCs-SF scaffold facilitated superior morphological restoration, with bladder tissue progressively adopting a more normal shape and no incidence of bladder calculus. Histological analysis revealed that the ADSCs-SF scaffold significantly promoted the regeneration of a more organized urothelium layer and smooth muscle tissue. It also resulted in higher vessel density and reduced infiltration of inflammatory cells when compared to the SF scaffold alone. Additionally, the ADSCs-SF group exhibited enhanced expression of key markers, including uroplakin III, a urothelial marker, and α-SMA, a smooth muscle cell marker. These findings suggest that the ADSCs-SF scaffold not only supports the structural integrity of the bladder but also improves tissue regeneration and reduces adverse inflammatory responses, offering a promising approach for bladder repair and reconstruction.

Evan S. Ross, Lance C. Visser, Lalida Tantisuwat et al.

ABSTRACT Background The effects of general anesthesia (GA) on less flow‐dependent (velocity ratio, velocity time integral [VTI] ratio and indexed pulmonary valve area [iPVA]) and flow‐dependent (mean [PVmeanPG] and maximum pressure gradient [PVmaxPG]) indices of severity of pulmonary stenosis (PS) are unclear. Objectives Determine the effects of GA on indices of severity of PS in dogs undergoing an interventional procedure (IP). Determine the reproducibility of indices of severity of PS. Animals Thirty‐nine dogs with PS. Methods Prospective cross‐sectional study. Five repeated echocardiograms were performed over 3 days. Day 1: two echocardiograms were performed by 2 different operators. Day 2: echocardiograms were performed before and after GA but before IP. Day 3: an echocardiogram was performed after the IP. Results After GA, median (IQR) cardiac index (2.1 [1.6–2.6] L/min/m2), PVmeanPG (45.0 [26.0–55.2] mmHg), PVmaxPG (76.6 [46.6–100.3] mmHg) were decreased (p ≤0.001) compared to before GA (2.8 [2.2–3.0] L/min/m2, 55.9 [47.6–73.1] mmHg, 96.1 [81.6–127.0] mmHg, respectively). There were no differences (p ≥0.35) in velocity ratio, VTI ratio, or iPVA after GA. Intra‐operator and inter‐operator coefficients of variation (95% CI) were highest for iPVA (13.8% [10.4–18.4] and 13.5% [11.0–18.4], respectively) and lowest for velocity ratio (9.2% [7.7–12.3] and 9.3% [7.7–12.4], respectively). Conclusions and Clinical Importance PVmeanPG and PVmaxPG might be misleading in states of reduced flow. An integrative assessment of severity of PS that includes less flow‐dependent indices is recommended. Reproducibility of indices of severity of PS should be considered when re‐evaluating dogs with PS.

Waheed Ezzat, Khalid M. Mahrose, Ahmed M. Rizk et al.

The exploration for effective in-feed additives is growing owing to the global climatic change trend to alleviate the negative effects of heat stress in laying hens. This research assessed the potential of using B-glucan (G) as an antiheat stress agent in Matrouh laying hens subjected to early heat shock programs during the growing period. Factorial design (3 × 3) was used, including 3 levels of heat stress (control, heat shock at 3 d and at 3 d and 8 wk of age) and 3 levels of β-glucan (0, 100, and 200 mg β-glucan /kg diet). During the first 12 wk of egg production (EP), treatments were exposed to heat challenge. The results revealed that heat shock program applications at 3 d and 8 wk of age significantly decreased body weight at 36 wk of age (P < 0.05) and reduced (P < 0.05) feed intake (FI). While significantly (P < 0.05) improved feed conversion ratio (FCR), hemoglobin, RBCs, WBCs, immunoglobulin M (IgM), immunoglobulin G (IgG), and Heat shock protein (HSP70) of the Liver (P < 0.01) as compared with the control group. At the same time, there was a decrease in lymphocyte%, H/L ratio, cortisol, and T3 compared to the thermo-neutral control. When compared to the control group, hens fed a diet containing 200 mg of βG significantly (P < 0.05) improved body weight at 16 wk and final weight at 36 wk, feed conversion (FCR) (g. feed/g. egg mass), hen-day egg production, and egg mass, as well as the digestibility coefficients of crude protein (CP), dry matter (DM), metabolizable energy (ME), and cortisol. The interactions between heat chock programs and βG levels were nonsignificant for the most studied traits except daily feed intake. Therefore, the early heat shock exposure 2 times and supplementation of Β-glucan (βG) at 200 mg/kg diet during the growth period for laying hens that are exposed to heat stress during the reproductive period could improve productive, reproductive performance, HSP70 level and enhance immunity responses.

Laurence Daigle, Hattaw Khalid, Carl A. Gagnon et al.

Abstract Background The primary objective of this cross-sectional study, conducted in Québec and Bristish Columbia (Canada) between February 2021 and January 2022, was to measure the prevalence of viral RNA in oronasal and rectal swabs and serum antibodies to severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) amongst cats living in households with at least one confirmed human case. Secondary objectives included a description of potential risk factors for the presence of SARS-CoV-2 antibodies and an estimation of the association between the presence of viral RNA in swabs as well as SARS-CoV-2 antibodies and clinical signs. Oronasal and rectal swabs and sera were collected from 55 cats from 40 households at most 15 days after a human case confirmation, and at up to two follow-up visits. A RT-qPCR assay and an ELISA were used to detect SARS-CoV-2 RNA in swabs and serum SARS-CoV-2 IgG antibodies, respectively. Prevalence and 95% Bayesian credibility intervals (BCI) were calculated, and associations were evaluated using prevalence ratio and 95% BCI obtained from Bayesian mixed log-binomial models. Results Nine (0.16; 95% BCI = 0.08–0.28) and 38 (0.69; 95% BCI = 0.56–0.80) cats had at least one positive RT-qPCR and at least one positive serological test result, respectively. No risk factor was associated with the prevalence of SARS-CoV-2 serum antibodies. The prevalence of clinical signs suggestive of COVID-19 in cats, mainly sneezing, was 2.12 (95% BCI = 1.03–3.98) times higher amongst cats with detectable viral RNA compared to those without. Conclusions We showed that cats develop antibodies to SARS-CoV-2 when exposed to recent human cases, but detection of viral RNA on swabs is rare, even when sampling occurs soon after confirmation of a human case. Moreover, cats with detectable levels of virus showed clinical signs more often than cats without signs, which can be useful for the management of such cases.

Gabriela de Assis dos Santos, João Manoel Magalhães Almeida Bezerra, Ana Beatriz Santana Silva et al.

O megaesôfago é uma enfermidade ocasionada pela hipomotilidade e dilatação esofágica parcial ou total, podendo ser congênito ou adquirido e subdivididos em primário, secundário, ou idiopático. Na forma congênita a patogenia não está totalmente esclarecida, mas, a sintomatologia inicia-se após o desmame e o filhote da ninhada apresenta subdesenvolvimento. O principal sinal clínico desta doença é a regurgitação após a ingestão de alimento e de água. O diagnóstico definitivo baseia-se no histórico, exame clínico e exames complementares de imagem. O tratamento é conservador, sendo necessário mudar o manejo alimentar e tratar as complicações que podem ser ocasionadas. Além disso, os cães mais acometidos são os médio a grande porte. Sendo assim, o objetivo deste trabalho é relatar um caso de um megaesôfago congênito em cão da raça Pinscher, filhote, com histórico de regurgitação desde o desmame, apetite voraz e retardo no desenvolvimento. Após o diagnóstico através de radiografia simples e contrastada foi iniciado o manejo alimentar e o paciente demonstrou melhora clínica.

Manuel Collado-Cuadrado, Claudia Alarcón-Torrecillas, Alfonso Balmori-de la Puente et al.

Heartworm disease, caused by <i>Dirofilaria immitis,</i> is a vector-borne zoonotic disease, (mainly affecting canids and felids) causing chronic vascular and pulmonary pathology in its early stages, which worsens with parasite load and/or death of adult worms in the pulmonary artery or right heart cavity, and can be fatal to the host. Angiogenesis is a mechanism by which new blood vessels are formed from existing ones. The aim of this work was to study the effect of two molecules of the <i>D. immitis</i> excretory/secretory antigen (DiES) on the angiogenic process, taking into account that this antigen is able to interact with this process and use it as a survival mechanism. For this purpose, an in vitro model of endothelial cells was used and treated with two recombinant proteins, i.e., actin (Act) and fructose-bisphosphate aldolase (FBAL) proteins belonging to DiES, and both pro- and antiangiogenic molecules were analyzed, as well as the cellular processes of cell proliferation, migration, and pseudocapillary formation. Act and FBAL proteins, together with vascular endothelial growth factor (VEGF-A), as an angiogenic precursor, are able to stimulate the production of proangiogenic factors as well as cellular processes of proliferation, migration, and pseudocapillary formation. This implies that these molecules could be produced by <i>D. immitis</i> to facilitate its survival, and the relationship between parasite and canine host would be further elaborated.

Andrea Mazzatenta, Serena Fiorito, Roberto Guadagnini et al.

The evanescent and invisible communication carried by chemical signals, pheromones, or signature mixtures or, as we prefer, the pheromonal individual fingerprint, between members of the same species is poorly studied in mammals, mainly because of the lack of identification of the molecules. The difference between pheromones and the pheromonal individual fingerprint is that the former generate stereotyped innate responses while the latter requires learning, i.e., different receivers can learn different signature mixtures from the same individual. Furthermore, pheromones are usually produced by a particular gland, while the pheromonal individual fingerprint is the entire bouquet produced by the entire secreting gland of the body. In the present study, we aim to investigate the pheromonal individual fingerprint of brown bears in northern Italy. We collected the entire putative pheromone bouquet from all production sites in free-ranging bears and analyzed the entire crude extract to profile the individual fingerprint according to species-, sex- and subjective-specific characteristics. We were able to putatively characterize the brown bears’ pheromonal individual fingerprints and compare them with the partial pheromone identifications published by other studies. This work is a step forward in the study of the complexity of chemical communication, particularly in a solitary endangered species.

D. Guest, J. Dudhia, R. Smith et al.

Citation: Guest DJ, Dudhia J, Smith RKW, Roberts SJ, Conzemius M, Innes JF, Fortier LA and Meeson RL (2022) Position Statement: Minimal Criteria for Reporting Veterinary and Animal Medicine Research for Mesenchymal Stromal/Stem Cells in Orthopedic Applications. Front. Vet. Sci. 9:817041. doi: 10.3389/fvets.2022.817041 Position Statement: Minimal Criteria for Reporting Veterinary and Animal Medicine Research for Mesenchymal Stromal/Stem Cells in Orthopedic Applications

Brayan Alexander Fonseca Martinez, V. Leotti, G. S. Silva et al.

One of the most commonly observational study designs employed in veterinary is the cross-sectional study with binary outcomes. To measure an association with exposure, the use of prevalence ratios (PR) or odds ratios (OR) are possible. In human epidemiology, much has been discussed about the use of the OR exclusively for case–control studies and some authors reported that there is no good justification for fitting logistic regression when the prevalence of the disease is high, in which OR overestimate the PR. Nonetheless, interpretation of OR is difficult since confusing between risk and odds can lead to incorrect quantitative interpretation of data such as “the risk is X times greater,” commonly reported in studies that use OR. The aims of this study were (1) to review articles with cross-sectional designs to assess the statistical method used and the appropriateness of the interpretation of the estimated measure of association and (2) to illustrate the use of alternative statistical methods that estimate PR directly. An overview of statistical methods and its interpretation using the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) guidelines was conducted and included a diverse set of peer-reviewed journals among the veterinary science field using PubMed as the search engine. From each article, the statistical method used and the appropriateness of the interpretation of the estimated measure of association were registered. Additionally, four alternative models for logistic regression that estimate directly PR were tested using our own dataset from a cross-sectional study on bovine viral diarrhea virus. The initial search strategy found 62 articles, in which 6 articles were excluded and therefore 56 studies were used for the overall analysis. The review showed that independent of the level of prevalence reported, 96% of articles employed logistic regression, thus estimating the OR. Results of the multivariate models indicated that logistic regression was the method that most overestimated the PR. The findings of this study indicate that although there are methods that directly estimate PR, many studies in veterinary science do not use these methods and misinterpret the OR estimated by the logistic regression.

N. Bruni, M. Capucchio, E. Biasibetti et al.

Antimicrobial peptides (AMPs) represent a vast array of molecules produced by virtually all living organisms as natural barriers against infection. Among AMP sources, an interesting class regards the food-derived bioactive agents. The whey protein lactoferrin (Lf) is an iron-binding glycoprotein that plays a significant role in the innate immune system, and is considered as an important host defense molecule. In search for novel antimicrobial agents, Lf offers a new source with potential pharmaceutical applications. The Lf-derived peptides Lf(1–11), lactoferricin (Lfcin) and lactoferrampin exhibit interesting and more potent antimicrobial actions than intact protein. Particularly, Lfcin has demonstrated strong antibacterial, anti-fungal and antiparasitic activity with promising applications both in human and veterinary diseases (from ocular infections to osteo-articular, gastrointestinal and dermatological diseases).

J. Prescott

ABSTRACT This chapter briefly reviews the history and current use of antimicrobials in animals, with a focus on food animals in the more economically developed countries. It identifies some of the differences between human medical and food animal use, particularly in growth promotional and “subtherapeutic” use of medically-important antibiotics in animals. The public health impact of the extensive use of antibiotics in food animals for these purposes, differences internationally in such usage, and the major changes in current practices now underway in agricultural use are summarized. The emerging framing of the dimensions of antimicrobial resistance within a “One Health” framework is focusing global efforts to address the antimicrobial resistance crisis in a collaborative manner. The rapidly evolving development and application of practices of antimicrobial stewardship in animal is a critical part of the huge global effort to address antimicrobial resistance. The outcome is still uncertain.

Jana Riehl, Ramesh Rijal, Leonie Nitz et al.

The abundant homohexameric AAA + ATPase p97 (also known as valosin-containing protein, VCP) is highly conserved from Dictyostelium discoideum to human and a pivotal factor of cellular protein homeostasis as it catalyzes the unfolding of proteins. Owing to its fundamental function in protein quality control pathways, it is regulated by more than 30 cofactors, including the UBXD protein family, whose members all carry an Ubiquitin Regulatory X (UBX) domain that enables binding to p97. One member of this latter protein family is the largely uncharacterized UBX domain containing protein 9 (UBXD9). Here, we analyzed protein-protein interactions of D. discoideum UBXD9 with p97 using a series of N- and C-terminal truncation constructs and probed the UBXD9 interactome in D. discoideum. Pull-down assays revealed that the UBX domain (amino acids 384–466) is necessary and sufficient for p97 interactions and that the N-terminal extension of the UBX domain, which folds into a β0-α–1-α0 lariat structure, is required for the dissociation of p97 hexamers. Functionally, this finding is reflected by strongly reduced ATPase activity of p97 upon addition of full length UBXD9 or UBXD9261–573. Results from Blue Native PAGE as well as structural model prediction suggest that hexamers of UBXD9 or UBXD9261–573 interact with p97 hexamers and disrupt the p97 subunit interactions via insertion of a helical lariat structure, presumably by destabilizing the p97 D1:D1’ intermolecular interface. We thus propose that UBXD9 regulates p97 activity in vivo by shifting the quaternary structure equilibrium from hexamers to monomers. Using three independent approaches, we further identified novel interaction partners of UBXD9, including glutamine synthetase type III as well as several actin-binding proteins. These findings suggest a role of UBXD9 in the organization of the actin cytoskeleton, and are in line with the hypothesized oligomerization-dependent mechanism of p97 regulation.

P. Deplazes, J. Eckert, A. Mathis et al.

V.A. Gushchina, А.А. Volodkin, N.I. Ostroborodova et al.

Currently, an urgent task is to find environmentally friendly and economically accessible energy sources for multi-purpose use. One such resource is a perennial plant from the bluegrass family, Miscanthus giganteus, which has a significant increase in above-ground mass by C4-photosynthesis, but cannot realize its potential as a crop with high rates of photosynthetic activity, because in the year of planting it competes poorly with weeds. Therefore, we studied ways to control the weed component in the agrocenosis of miscanthus on the light gray soil of the collection area of Penza State Agrarian University. In the years of the research, characterized by different hydrothermal conditions (HTC 0.64-1.29), it was found that the herbicides of systemic action Balerina and Magnum in the background of Tornado 500 contribute to an increase in the leaf area of miscanthus in the first year up to 18.36...19.09 thousand m2/ha, in the photosynthetic potential to 496.1...508.7 thousand m2∙day/ha. The maximum values of net productivity of photosynthesis (0.62...0.85 g/m2∙day) were observed at the end of June. Due to the double chemical weeding the decrease of weeds leads to the maximum yield of above-ground mass – 8.92...9.09 t/ha. The highest dry matter yield of 2.13...2.97 t/ha was obtained using the Tornado 500 in combination with the herbicides Balerina and Magnum.

Carina Strohmayer, Andrea Klang, Sibylle Kneissl

Squamous cell carcinoma (SCC) is the most common equine sinonasal and feline oral tumour. This study aimed to describe the computed tomographic and histopathological characteristics of equine and feline SCC. Thirteen horses and 10 cats that had been histopathologically diagnosed with oral or sinonasal SCC and had undergone computed tomography (CT) of the head were retrospectively included in the study. CT characteristics of the mass and involved structures were noted. Histological examinations were evaluated according to a human malignancy grading system for oral SCC, which considered four grades of increasing aggressiveness. In horses, the masses were at the levels of the paranasal sinuses (n = 8), mandible (n = 3), tongue (n = 1), and nasal cavity (n = 1). In cats, the masses were at the levels of the maxilla (n = 4), mandible (n = 3), tongue (n = 1), and buccal region (n = 1) and were diffusely distributed (facial and cranial bones; n = 1). Masses in the equine paranasal sinuses showed only mild, solid/laminar, periosteal reactions with variable cortical destruction. However, maxillary lesions in cats showed severe cortical destruction and irregular, amorphous/pumice stone-like, periosteal reactions. CT revealed different SCC phenotypes that were unrelated to the histological grade. For morphologic parameters of the tumour cell population, a variability for the degree of keratinization and number of mitotic cells was noted in horses and cats. Concerning the tumour-host relationship a marked, extensive and deep invasion into the bone in the majority of horses and cats was seen. Most cases in both the horses and cats were categorized as histological grade III (n = 8); four horses and one cat were categorized as grade IV, and one horse and one cat were categorized as grade II. In this study, we examined the diagnostic images and corresponding applied human histopathological grading of SCC to further elucidate the correlations between pathology and oral and sinonasal SCC imaging in horses and cats.

E. Michalová, PNovotna, J. Schlegelova

C. Bertram, R. Klopfleisch

Jannigje G. Kers, Egil A.J. Fischer, J. Arjan Stegeman et al.

In the short life of broiler chickens, their intestinal microbiota undergoes many changes. To study underlying biological mechanisms and factors that influence the intestinal microbiota development, longitudinal data from flocks and individual birds is needed. However, post-mortem collection of samples hampers longitudinal data collection. In this study, invasively collected cecal and ileal content, cloacal swabs collected from the same bird, and boot sock samples and cecal droppings from the litter of the broilers’ poultry house, were collected on days 0, 2, 7, 14 and 35 post-hatch. The different sample types were evaluated on their applicability and reliability to characterize the broiler intestinal microbiota. The microbiota of 247 samples was assessed by 16S ribosomal RNA gene amplicon sequencing. Analyses of α and β measures showed a similar development of microbiota composition of cecal droppings compared to cecal content. Furthermore, the composition of cecal content samples was comparable to that of the boot socks until day 14 post-hatch. This study shows that the value of non-invasive sample types varies at different ages and depends on the goal of the microbiota characterization. Specifically, cecal droppings and boot socks may be useful alternatives for cecal samples to determine intestinal microbiota composition longitudinally.

Lindsay Hochman

Laser therapy, or photobiomodulation, has rapidly grown in popularity in human and veterinary medicine. With a number of proposed indications and broad, sometimes anecdotal, use in practice, research interest has expanded aimed at providing scientific support. Recent studies have shown that laser therapy alters the inflammatory and immune response as well as promotes healing for a variety of tissue types. This review will cover the history of the modality, basic principles, proposed mechanisms of action, evidence-based clinical indications, and will guide the practitioner through its application in practice.