Hasil untuk "Human anatomy"

Feng Yuan, Zilan Zhong, Rui Qin et al.

BACKGROUND: We aimed to study the anatomy of the Chinese occipital condyle and its position relative to the occipital foramen and skull. MATERIALS AND METHODS: Measurements were taken from 106 adult Chinese skulls using a Cartesian coordinate system centred on the foramen magnum. Measurements included the longitudinal diameter of the foramen magnum, distances from various points on the occipital condyles to the foramen magnum and skull landmarks, and the occipital condyle classification index (OCI) and skull-occipital condyle classification index (SOCI). RESULTS: Occipital condyle index classified the position of the foramen magnum and occipital condyles into three groups: OCI ≤ 0.40 (3 cases, 2.83%); 0.40 < OCI ≤ 0.50 (75 cases, 70.75%); and OCI > 0.50 (28 cases, 26.42%). SOCI classified the relationship between the skull and occipital condyles into two groups: 0.5 < SOCI ≤ 0.6 (49 cases, 46.23%); and 0.6 < SOCI ≤ 0.7 (57 cases, 53.77%). Four relationship types were identified based on specific measurements: Type I (23 cases, 21.70%); Type II (42 cases, 39.62%); Type III (four cases, 3.77%); and Type IV (37 cases, 34.91%). CONCLUSIONS: Sagittal movement of the occipital condyle affects the cervical spine’s curvature. Asymmetry between the occipital condyles and the foramen magnum can misalign the skull with the body’s coronal plane.

Dengfeng Ding, Yuqiong Zhao, Yunxiao Jia et al.

Abstract Background Atherosclerosis is a chronic cardiovascular disease of great concern. However, it is difficult to establish a direct connection between conventional small animal models and clinical practice. The pig's genome, physiology, and anatomy reflect human biology better than other laboratory animals, which is crucial for studying the pathogenesis of atherosclerosis. Methods We used whole‐genome sequencing data from nine Bama minipigs to perform a genome‐wide linkage analysis, and further used bioinformatic tools to filter and identify underlying candidate genes. Candidate gene function prediction was performed using the online prediction tool STRING 12.0. Immunohistochemistry and immunofluorescence were used to detect the expression of proteins encoded by candidate genes. Results We mapped differential single nucleotide polymorphisms (SNPs) to genes and obtained a total of 102 differential genes, then we used GO and KEGG pathway enrichment analysis to identify four candidate genes, including SLA‐1, SLA‐2, SLA‐3, and TAP2. nsSNPs cause changes in the primary and tertiary structures of SLA‐I and TAP2 proteins, the primary structures of these two proteins have undergone amino acid changes, and the tertiary structures also show slight changes. In addition, immunohistochemistry and immunofluorescence results showed that the expression changes of TAP2 protein in coronary arteries showed a trend of increasing from the middle layer to the inner layer. Conclusions We have identified SLA‐I and TAP2 as potential susceptibility genes of atherosclerosis, highlighting the importance of antigen processing and immune response in atherogenesis.

Yashar Iran Pour, Afrooz Zandifar

Diaphragmatic ultrasound, valued for its portability and safety, assesses both structural and functional aspects of the diaphragm. While some studies support its predictive value, others conflict. This meta-analysis aims to clarify diaphragmatic ultrasound's role in predicting successful liberation from mechanical ventilation in intensive care settings. A systematic search was performed on Web of Science, Scopus, and PubMed up to March, 2024. The search strategy included a combination of relevant medical subject heading (MeSH) terms and relevant keywords. We defined our eligibility criteria based on the PICO framework. Two authors performed the data extraction using a standardized sheet. The pooled mean difference was calculated using random effects model and Hedges’ g along with SD estimation. R and RStudio were used for the statistical analysis and creating forest and funnel plots. The pooled mean difference was 7.25 (95% CI: 4.20, 10.21) for DE among the two groups. We found a statistically significant difference among the two groups indicating that those with successful weaning from intubation had higher means of DE compared to those with failed weaning attempt (p-value<0.01). The mean difference of DTF was also higher among those with successful weaning from intubation compared to those with failed weaning attempt with the pooled mean difference of 14.52 (95% CI: 10.51, 18.54, p-value<0.01). The mean difference of RSBI was lower among those with successful weaning from intubation compared to those with failed weaning attempt with the pooled mean difference of -28.86 (95% CI: -41.82, -15.91, p-value<0.01). Our results suggest that evaluating diaphragmatic excursion and thickening fraction can reliably anticipate successful liberation from mechanical ventilation. However, significant heterogeneity was present among the included studies. High-quality research, particularly randomized clinical trials, is required to further elucidate the role of diaphragmatic ultrasound in predicting weaning from mechanical ventilation.

Li J, Zhou T, Wang P et al.

Juan Li,1,&ast; Ting Zhou,1,&ast; Pei Wang,1,&ast; Ruian Yin,1 Shengqi Zhang,1 Yile Cao,1 Lijuan Zong,1 Ming Xiao,2 Yongjie Zhang,3 Wentao Liu,4 Lingxiao Deng,5 Fei Huang,6 Jianfei Sun,7,&ast; Hongxing Wang1,&ast; 1Department of Rehabilitation Medicine, Zhongda Hospital Southeast University, Nanjing, 210024, People’s Republic of China; 2Jiangsu Key Laboratory of Neurodegeneration, Nanjing Medical University, Nanjing, 211166, People’s Republic of China; 3Department of Human Anatomy, Nanjing Medical University, Nanjing, 211166, People’s Republic of China; 4Department of Pharmacology, Jiangsu Key Laboratory of Neurodegeneration, Nanjing Medical University, Nanjing, 211166, People’s Republic of China; 5Department of Neurological Surgery, Spinal Cord and Brain Injury Research Group, Stark Neurosciences Research Institute, Indianapolis, IN, 46202-2266, USA; 6Institute of Neurobiology, Binzhou Medical University, Yantai, 264003, People’s Republic of China; 7State Key Laboratory of Bioelectronics, Jiangsu Key Laboratory of Biomaterials and Devices, School of Biological Science and Medical Engineering, Southeast University, Nanjing, 210009, People’s Republic of China&ast;These authors contributed equally to this workCorrespondence: Jianfei Sun, State Key Laboratory of Bioelectronics, Jiangsu Key Laboratory of Biomaterials and Devices, School of Biological Science and Medical Engineering, Southeast University, Nanjing, 210009, People’s Republic of China, Email sunzaghi@seu.edu.cn Hongxing Wang, Department of Rehabilitation Medicine, Southeast University Zhongda Hospital, Nanjing, 210024, People’s Republic of China, Email 101012648@seu.edu.cnBackground: Gigantocellular reticular nucleus (GRNs) executes a vital role in locomotor recovery after spinal cord injury. However, due to its unique anatomical location deep within the brainstem, intervening in GRNs for spinal cord injury research is challenging. To address this problem, this study adopted an extracorporeal magnetic stimulation system to observe the effects of selective magnetic stimulation of GRNs with iron oxide nanoparticles combined treadmill training on locomotor recovery after spinal cord injury, and explored the possible mechanisms.Methods: Superparamagnetic iron oxide (SPIO) nanoparticles were stereotactically injected into bilateral GRNs of mice with moderate T10 spinal cord contusion. Eight-week selective magnetic stimulation produced by extracorporeal magnetic stimulation system (MSS) combined with treadmill training was adopted for the animals from one week after surgery. Locomotor function of mice was evaluated by the Basso Mouse Scale, Grid-walking test and Treadscan analysis. Brain MRI, anterograde virus tracer and immunofluorescence staining were applied to observe the tissue compatibility of SPIO in GRNs, trace GRNs’ projections and evaluate neurotransmitters’ expression in spinal cord respectively. Motor-evoked potentials and H reflex were collected for assessing the integrity of cortical spinal tract and the excitation of motor neurons in anterior horn.Results: (1) SPIO persisted in GRNs for a minimum of 24 weeks without inducing apoptosis of GRN cells, and degraded slowly over time. (2) MSS-enabled treadmill training dramatically improved locomotor performances of injured mice, and promoted cortico-reticulo-spinal circuit reorganization. (3) MSS-enabled treadmill training took superimposed roles through both activating GRNs to drive more projections of GRNs across lesion site and rebalancing neurotransmitters’ expression in anterior horn of lumbar spinal cord.Conclusion: These results indicate that selective MSS intervention of GRNs potentially serves as an innovative strategy to promote more spared fibers of GRNs across lesion site and rebalance neurotransmitters’ expression after spinal cord injury, paving the way for the structural remodeling of neural systems collaborating with exercise training, thus ultimately contributing to the reconstruction of cortico-reticulo-spinal circuit. Keywords: gigantocellular reticular nucleus, locomotion, magnetic stimulation, spinal cord injury, superparamagnetic iron oxide nanoparticles, treadmill training

Yuxiang Zhan, Minjie Yang, Shuoqiu Bai et al.

AbstractContext: A comprehensive meta-analysis was carried out to investigate the impact of orthodontics on masticatory muscles.Methods: A thorough search of various databases, including CNKI, Wan Fang, VIP, CBM, MEDLINE, PubMed, Cochrane Library, EMBASE, Web of Science, and Google Scholar, was performed to identify relevant studies on patients undergoing orthodontics or functional corrections. Six case-control studies were finally included in this analysis, which specifically examined the effect of orthodontic treatment on masticatory muscle function.Results: The results revealed that the mean masticatory muscle voltage in patients treated with orthodontics was found to be higher after treatment compared to before treatment [odds ratio (OR)=1.57, 95% confidence interval (CI) (0.57, 2.57), p = 0.002], which could potentially have an impact on masticatory muscle function, particularly in individuals with Class II Division 1 malocclusion.Conclusion: These findings contribute to our understanding of the effects of orthodontic interventions on masticatory muscles, further highlighting the importance of orthodontics in optimising masticatory function.

Kalpana Rangra, Virender Kadyan, Monit Kapoor

Speech is an effective way for analyzing mental and psychological health of a speaker's. Automatic speech recognition has been efficiently investigated for human-computer interaction and understanding the emotional &amp; psychological anatomy of human behavior. Emotions and personality are studied to have a strong link while analyzing the prosodic speech parameters. The work proposes a novel personality and emotion classification model using PSO (particle swarm optimization) based CNN (convolution neural network): (NPSO) that predicts both (emotion and personality) The model is computationally efficient and outperforms language models. Cepstral speech features MFCC (mel frequency cepstral constants) is used to predict emotions with 90% testing accuracy and personality with 91% accuracy on SAVEE(Surrey Audio-Visual Expressed Emotion) individually. The correlation between emotion and personality is identified in the work. The experiment uses the four corpora SAVEE, RAVDESS (Ryerson Audio-Visual Database of Emotional Speech and Song), CREMAD (Crowd-sourced Emotional Multimodal Actors Dataset, TESS (Toronto emotional speech set) corpus, and the big five personality model for finding associations among emotions and personality traits. Experimental results show that the classification accuracy scores for combined datasets are 74% for emotions and 89% for Personality classifications. The proposed model works on seven emotions and five classes of personality. Results prove that MFCC is enough effective in characterizing and recognizing emotions and personality simultaneously.

Gaetano Scaramuzzo, Francesco Nucera, Alessio Asmundo et al.

Abstract The severe acute respiratory syndrome-coronavirus-2 (SARS-CoV-2) infection can be asymptomatic or cause a disease (COVID-19) characterized by different levels of severity. The main cause of severe COVID-19 and death is represented by acute (or acute on chronic) respiratory failure and acute respiratory distress syndrome (ARDS), often requiring hospital admission and ventilator support. The molecular pathogenesis of COVID-19-related ARDS (by now termed c-ARDS) is still poorly understood. In this review we will discuss the genetic susceptibility to COVID-19, the pathogenesis and the local and systemic biomarkers correlated with c-ARDS and the therapeutic options that target the cell signalling pathways of c-ARDS.

S.A. Adelakun, V.O. Ukwenya, A.O. Ojewale et al.

Background: Sodium fluoride (NaF) causes testicular toxicity via an oxidative stress mechanism. Solanum aethiopicum (SAE) contains abundant antioxidant and androgenic properties. This study investigated the ameliorating potential of SAE extract against NaF-induced testicular damage in rats. Methods: Forty-eight (48) adult male Sprague Dawley rats (130–180 g, 7–9 weeks old) were randomized into six groups of eight rats (n = 8) each. A: Control received only deionized water, B: 20 mg/kg body weight (bw) NaF. C: 500 mg/kg bw SAE. D: 20 mg/kg bw NaF + 500 mg/kg bw SAE. E: 20 mg/Kg bw NaF + 30 mg/kg bw vitamin C (Vit. C). F: 20 mg/kg bw NaF + 500 mg/kg bw SAE + 30 mg/kg bw Vit. C orally for 8 weeks. Testicular histology, histomorphometry parameters, sperm parameters, luteinizing hormone, follicle-stimulating hormone, testosterone, reduced glutathione, glutathione peroxidase, catalase, superoxide dismutase, malondialdehyde (MDA) and inflammation markers were analyzed. Results: Sodium fluoride significantly decrease sperm quality, hormones, antioxidants concentrations, and general body weights and significantly increased MDA, and cause disorientation of seminiferous tubules with a nearly empty lumen and vascular hemorrhage but was attenuated with SAE and Vit. C. Conclusion: Solanum aethiopicum possesses pro-fertility properties capable of ameliorating oxidative stress-induced testicular damage with the profound significance that comes with Vit. C combination.

Zdenko Červenák, Filip Červenák, Marian Baldovič et al.

Background Short tandem repeats (STRs) are genetic markers frequently used for human identification and paternity testing. They are highly mutable, which may occasionally lead to inconsistencies between the genotypes of parents and their children. As the mutation rates of individual STR markers can vary among populations, population-specific data are of high importance. Aim To investigate the mutation rates of 16 STR markers in the Slovak population. Subjects and methods In this study, we analysed the germline mutation rates of 16 STR markers (TH01, D3S1358, vWA, D21S11, D16S539, D1S1656, D19S433, SE33, D10S1248, D22S1045, D12S391, D8S1179, D2S1338, D2S441, D18S51 and FGA) in the Slovak population. At these loci, we analysed 42 096 allelic transfers and identified 61 mutation events. Results The loci with the highest overall mutation rates were SE33 and FGA, while no mutations were identified in TH01, D19S433 and D22S1045. The average paternal mutation rate was higher than the maternal mutation rate. All but one mutation consisted of gains or losses of a single repeat unit and the overall mutation rate was estimated to be 1.45 x10−3 per meiosis. Conclusion This study provides data which can be used to further strengthen the correct paternity index calculations and reliability of paternity testing in Slovakia.

Jon Jatsu Azkue

AbstractOpportunities for clinicians, researchers, and medical students to become acquainted with the three‐dimensional (3D) anatomy of the human embryo have historically been limited. This work was aimed at creating a collection of digital, printable 3D surface models demonstrating major morphogenetic changes in the embryo's external anatomy, including typical features used for external staging. Twelve models were digitally reconstructed based on optical projection tomography, high‐resolution episcopic microscopy and magnetic resonance imaging datasets of formalin‐fixed specimens of embryos of developmental stages 12 through 23, that is, stages following longitudinal and transverse embryo folding. The reconstructed replica reproduced the external anatomy of the actual specimens in great detail, and the progress of development over stages was recognizable in a variety of external anatomical features and bodily structures, including the general layout and curvature of the body, the pharyngeal arches and cervical sinus, the physiological gut herniation, and external genitalia. In addition, surface anatomy features commonly used for embryo staging, such as distinct steps in the morphogenesis of facial primordia and limb buds, were also apparent. These digital replica, which are all provided for 3D visualization and printing, can serve as a novel resource for teaching and learning embryology and may contribute to a better appreciation of the human embryonic development.

Andrea Bonicelli, Peter Zioupos, Emily Arnold et al.

Abstract Forensic anthropology includes, amongst other applications, the positive identification of unknown human skeletal remains. The first step in this process is an assessment of the biological profile, that is: sex, age, stature and ancestry. In forensic contexts, age estimation is one of the main challenges in the process of identification. Recently established admissibility criteria are driving researchers towards standardisation of methodological procedures. Despite these changes, experience still plays a central role in anthropological examinations. In order to avoid this issue, age estimation procedures (i) must be presented to the scientific community and published in peer reviewed journals, (ii) accurately explained in terms of procedure and (iii) present clear information about the accuracy of the estimation and possible error rates. In order to fulfil all these requirements, a number of methods based on physiological processes which result in biochemical changes in various tissue structures at the molecular level, such as modifications in DNA-methylation and telomere shortening, racemization of proteins and stable isotopes analysis, have been developed. The current work proposes a new systematic approach in age estimation based on tracing physicochemical and mechanical degeneration of the rib cortical bone matrix. This study used autopsy material from 113 rib specimens. A set of 33 parameters were measured by standard bio-mechanical (nanoindentation and microindentation), physical (TGA/DSC, XRD and FTIR) and histomorphometry (porosity-ImageJ) methods. Stepwise regressions were used to create equations that would produce the best ‘estimates of age at death’ vs real age of the cadavers. Five equations were produced; in the best of cases an equation counting 7 parameters had an R2 = 0.863 and mean absolute error of 4.64 years. The present method meets all the admissibility criteria previously described. Furthermore, the method is experience-independent and as such can be performed without previous expert knowledge of forensic anthropology and human anatomy.

A. Borghesi, M. P. Bondioni

The mandibular canal is a bony channel located within the spongiosa of the mandible. The main structure contained in the mandibular canal is the inferior alveolar nerve. The inferior alveolar nerve is a very important structure that requires due consideration during dental or surgical procedures involving the mandible. Therefore, a detailed morphological analysis of the mandibular canal should be carried out before any surgical procedure in the mandibular region in order to avoid complications and to reduce the risk of inadequate local nerve blocking. The human mandible typically has a single mandibular canal on each side; however, accessory mandibular canals have been described previously in the literature. The most common variant of the mandibular canal is the bifid mandibular canal, which has a prevalence ranging from 10% to 66% on cone-beam computed tomography (CBCT) examinations. A rare variant of bifid mandibular canal is the trifid canal, accounting for less than 6% of all bifid canals. In some cases, the bifid and trifid mandibular canals are associated with a double mandibular foramen, which is a rare anatomical variant with a reported incidence of 1.35% on CBCT images. Herein, we present the interesting CBCT images of an unexpected anatomical variant characterised by unilateral triple mandibular canal with double mandibular foramen in a young Caucasian woman.

Vladyslav Luzin, Artur Koch'yan, Natal'ya Zabolotnaya et al.

Antoine Micheau, Denis Hoa

A. Ekingen, E. S. Hatipoğlu, C. Hamidi et al.

Background: The splenic artery (SA) variations are rarely reported in the literature. Knowledge of the range of the SA and other arterial anomalies and their specific frequencies is very important ever for every visceral surgeon as well as for treatment of gastrointestinal bleeding, organ transplantation, transarterial chemoembolisation of neoplasm, infusion therapy, therapeutic arterial ligation, iatrogenic injuries. At the literature, there are more studies on the coeliac trunk, superior mesenteric artery and hepatic artery variations, but studies on the SA variations are uncommon. The studies on the SA variations are mostly in the form of case reports, but there are not many studies with large population on this issue. The purpose of this study was to evaluate the SA alone and to determine the variations determined separately from the other arteries. Accurate awareness of all the possible anatomic variations is crucial in the upper abdomen surgery. Materials and methods: Seven hundred fifty patients undergoing multi-detector computed tomography angiography between 2015 and 2017 were retrospectively evaluated for the SA variations. We created a new classification system to determine anatomic variations of the SA. Results: Twenty-three different types were identified related to anatomic variations in the origin and branching pattern of the SA. While 596 (79.47%) patients had standard SA anatomy, 154 (20.53%) patients had variant SA anatomy. Conclusions: The SA has quite different variation types and the practical context of the issue is of primary importance in surgery, gastroenterology, oncology and radiology. Liver and pancreas transplantation, splenectomy, embolisation of tumours of the abdominal organs, as well as other numerous diagnostic and therapeutic procedures, require detailed anatomical knowledge

Danielle Vaughan, Olli Ritvos, Robert Mitchell et al.

Numerous approaches are being developed to promote post-natal muscle growth based on attenuating Myostatin/Activin signalling for clinical uses such as the treatment neuromuscular diseases, cancer cachexia and sarcopenia. However there have been concerns about the effects of inhibiting Activin on tissues other than skeletal muscle. We intraperitoneally injected mice with the Activin ligand trap, sActRIIB, in young, adult and a progeric mouse model. Treatment at any stage in the life of the mouse rapidly increased muscle mass. However at all stages of life the treatment decreased the weights of the testis. Not only were the testis smaller, but they contained fewer sperm compared to untreated mice. We found that the hypertrophic muscle phenotype was lost after the cessation of sActRIIB treatment but abnormal testis phenotype persisted. In summary, attenuation of Myostatin/Activin signalling inhibited testis development. Future use of molecules based on a similar mode of action to promote muscle growth should be carefully profiled for adverse side-effects on the testis. However the effectiveness of sActRIIB as a modulator of Activin function provides a possible therapeutic strategy to alleviate testicular seminoma development.

Ritee Shrestha, Bipana Manandhar, Hari Prasad Upadhyay et al.

Introduction: Human nose is one of the important anthropometric parameters for identification of ethnicity and sex of an individual of an unknown identity. The nasal index holds a great value in anthropological studies, because it is one of the anthropometric indices acknowledged in nasal surgery as well as management.  The study aims to find the mean nasal index and the nose type of dental students of Kantipur Dental College of Nepal. Methods: This descriptive cross-sectional study was conducted among 140 dental students of age groups 17-25 years, in the Department of Anatomy, Kantipur Dental College Teaching Hospital and Research Center, Basundhara, Kathmandu. The studied population belongs to dental students of Kantipur Dental College and Research Centre. The nasal parameters including the nasal height, nasal width was measured using Digital Vernier Caliper and the nose was classified in three different types based on the value of nasal index. Results:   The mean nasal index of total population was 81.34±14.88 mm with confidence interval range of 78.85 and 83.83 mm. Mesorrhine type of nose was found to be most common among the total population. Mean nasal index in male is 84.49±12.46 mm and in female is 80.66±15.32 mm.   Conclusions: This study concludes that the mean values of nasal index of all the students falls under mesorrhine (medium) type of nose.

Denys SHYIAN, Olga AVILOVA, Irina LADNAYA

Background. Pollution of the environment poses major risks for human health. Chronic exposure to some well-absorbed but slowly eliminated xenobiotics can lead to their bioaccumulation in living organisms. The objective of the study was to describe the organometric changes occurring in thymus of male rats under tryglycidyl ether of polyoxypropylenetriol exposure. Material and methods. 40 WAG matured male rats were divided randomly into two groups. The first group served as a control included 8 animals. The second group of 32 rats, 8 rodents in each, were treated via gavage by aqueous solutions of tryglycidyl ether of polyoxypropylenetriol (TEPPT) in a dose of 1/10 LD50 in conversion to 5.75 g/kg during 7, 15, 30, 45 days. All animals were sacrificed on the term defined by experimental design. Thymus specimens were dissected out and measurements of the linear dimensions (length, width, height) using digital caliper were taken. The mass and volume of the thymus were counted. Limits of the thymus morphometric indices’ variability in intact and experimental groups were calculated. Results. The research indicates that tryglycidyl ether of polyoxypropylenetriol exposure caused marked organometric changes in rats’ thymus. 100% effect on all morphometric indices of the thymus under impact of TEEPT in a dose of 1/10 LD50 on the 7th, 15th, 30th and 45th day was noted. However, more pronounced changes were observed on 7th and 30th days. The study revealed that IndT of the control group, which is related to the length and width of the thymus, has the greatest limits of the parameters’ fluctuations and their significant variability. IndHL of the control group, which is associated with the height and length of the thymus, has the lowest fluctuation limits of the parameters. In our opinion, this is connected, first of all, with the peculiarities of the structure and form of the rats’ thymus. Conclusions. Thymus shows active response on the induced xenobiotic and these data can be successfully extrapolated from experimental animals to humans.

Billy A. Watson, Jennifer M. Feenstra, Jonathan M. Van Arsdale et al.

During limb development, fibroblast growth factors (Fgfs) govern proximal&ndash;distal outgrowth and patterning. FGFs also synchronize developmental patterning between the proximal&ndash;distal and anterior&ndash;posterior axes by maintaining Sonic hedgehog (Shh) expression in cells of the zone of polarizing activity (ZPA) in the distal posterior mesoderm. Shh, in turn, maintains Fgfs in the apical ectodermal ridge (AER) that caps the distal tip of the limb bud. Crosstalk between Fgf and Shh signaling is critical for patterned limb development, but the mechanisms underlying this feedback loop are not well-characterized. Implantation of Fgf beads in the proximal posterior limb bud can maintain SHH expression in the former ZPA domain (evident 3 h after application), while prolonged exposure (24 h) can induce SHH outside of this domain. Although temporally and spatially disparate, comparative analysis of transcriptome data from these different populations accentuated genes involved in SHH regulation. Comparative analysis identified 25 candidates common to both treatments, with eight linked to SHH expression or function. Furthermore, we demonstrated that LHX2, a LIM Homeodomain transcription factor, is an intermediate in the FGF-mediated regulation of SHH. Our data suggest that LHX2 acts as a competency factor maintaining distal posterior SHH expression subjacent to the AER.

Jaiswal Pratima, Verma Lakshmi, Ghulyani Tuhin

Background and aims: Surgeries involving the femur are quite common. The clinical importance of femur in sports biomechanics and rehabilitation is well known. Therefore, its morphometries has orthopedic implications. The present study aims at establishing interrelationship among various external parameters in adult dried femora including femoral offset (FO), neck shaft angle [NSA], femoral anteversion angle [FNA], Neck width [NW], Anterior Neck length [ANL], Posterior Neck length [PNL], Oblique length [OL] and trochanteric oblique length [TOL], Materials and Methods: A total of 100 [50 right and 50 left] intact, dried adult human femora from department of Anatomy, Government Medical College, Kota were included in this study. The FO, NSA and FNA were measured by digital image analysis in ‘IMAGEJ’ software. OL and TOL were measured using osteometric board. ANL, PNL and NW were recorded using sliding caliper. Statistical analysis was done using SPSS and Microsoft Excel software. The mean values were calculated, relationship between parameters was measured by Pearson’s correlation coefficient V. Multiple regression equations were derived in order to calculate FO by other variables. Results: FO showed strong negative correlation with NSA; strong positive correlation with ANL, PNL, NW; moderate positive correlation with OL, TOL; but no relationship with FNA. NSA had moderate negative correlation with NW and weak negative correlation with PNL. NW & ANL had positive correlation with OL, TOL. Conclusion: In our method we used dried, intact bone and digital photographs with standardised method. Therefore it should provide accurate results; in addition it is cost effective. However studies on larger scale are warranted to design implants for Indian population.