Hasil untuk "Industrial hygiene. Industrial welfare"

Lisa M. F. Janssen, Caroline de Ocampo, Dwight H. Kono et al.

Abstract Background The mucosal origins hypothesis of rheumatoid arthritis (RA) posits that inhalant exposures, such as cigarette smoke and crystalline silica (c-silica), trigger immune responses in the lungs that contribute to joint disease onset. However, the relationship between inhalants, lung inflammation, and inflammatory arthritis remains poorly understood. Results This study compared the development of inflammatory arthritis in two genetically susceptible mouse strains, the BXD2/TyJ (BXD2) and chimeric HLA-DR4-IE transgenic (DR4-Tg), following delivery of c-silica to the lungs via oropharyngeal aspiration. In BXD2 mice, c-silica exposure was associated with rapid arthritis development, marked by synovial cell hyperplasia, pannus formation, and severe erosion of cartilage and bone. These features were preceded by pulmonary inflammation characterized by lymphoid-like cell clusters lining vessels and bronchi which cell-specific immunofluorescent microscopy identified as organized lymphoid structures consistent with inducible bronchus-associated lymphoid tissue (iBALT). Inflammatory arthritis was also preceded by autoantibodies associated with RA and other systemic autoimmune diseases including anti-citrullinated protein autoantibodies (ACPA) and rheumatoid factor (RF) in bronchoalveolar lavage fluid (BALF). However, the most predominant autoantibodies in BALF were against extractable nuclear antigens (ENA). Anti-ENA were also prominent in serum and microarray autoantigen analysis confirmed the response as targeting components of Sm and RNP small nuclear ribonucleoproteins (snRNPs). In contrast, DR4-Tg mice had no signs of arthritis, milder lung inflammation lacking iBALT, and negligible autoantibody responses. Conclusion Genetic predisposition beyond HLA-DR4 alone is required for the immunological manifestations that lead to c-silica mediated inflammatory arthritis. These findings provide novel insights into the relationship between mucosal exposure and RA pathogenesis.

Huiwen Kang, Danyang Huang, Wei Zhang et al.

Abstract Background Micro-/nanoplastics (MNPLs) are widely found in the environment and have toxic effects on various organs and systems. However, the role of the gut-cardiac axis in cardiotoxicity induced by MNPLs has not yet been elucidated through research. Results In this study, we examined the effects of 80 nm polystyrene nanoplastics (PS-NPs) on the heart and human cardiomyocytes (AC16) cells. Histopathological examination showed that NPs caused impaired cardiac function and increased myocardial collagen deposition. In view of the potential influence of gut microbiota and its metabolites on cardiac function, we conduct this study to investigate the specific effects they have on cardiac function. Analysis of cecal contents by 16 s ribosomal RNA (rRNA) and short chain fatty acids (SCFAs) revealed that colonic tissue damage, intestinal flora disorder, and reduction of propionic acid induced by PS-MPs were closely related to cardiac function. Further transcriptomic analysis of heart and colon tissues indicated that propionic acid may reduce cardiac function by reducing the expression of fructose-1, 6-biphosphatase 1 (FBP1). The hypothesis was further verified by in vitro intervention experiments with sodium propionate and FBP1 activator (BML-275). Conclusions In summary, our study systematically demonstrated the role of gut-heart axis in NPs-induced cardiac injury, and the specific process was that NPs exposure reduced propionate level, which in turn inhibited FBP1 expression to impair cardiac function. These findings provide new insights into NPs-induced cardiotoxicity and identifie potential therapeutic targets, providing clues for the prevention and treatment of NPs-induced cardiac injury in the future. Graphical Abstract

Thomas Sandström, Jenny A. Bosson, Ala Muala et al.

Abstract Background Exposure to standard petrodiesel exhaust is linked to adverse health effects. Moreover, there is a mounting request to replace fossil-based fuels with renewable and sustainable alternatives and, therefore, rapeseed methyl ester (RME) and other biofuels have been introduced. However, recent toxicological research has indicated that biodiesel exhaust may also induce adverse health-related events. Aim To determine whether exposure to 100% RME biodiesel (BD100) exhaust would cause an acute airway neutrophilic recruitment in humans. Methods Fourteen healthy subjects underwent exposure to diluted BD100 exhaust and filtered air for 1-h, in a blinded, random fashion. Bronchoscopy with endobronchial mucosal biopsies, bronchial wash (BW) and bronchoalveolar lavage (BAL) was performed six hours after exposure. Differential cell counts and inflammatory markers were determined in the supernatant and biopsies were stained immunohistochemically. Results Compared with filtered air, BD100 exhaust exposure increased bronchial mucosal endothelial P-selectin adhesion molecule expression, as well as neutrophil, mast cell and CD68 + macrophage numbers. An increased influx of neutrophils and machrophages was also seen in BW. Conclusion Exposure to biodiesel exhaust was associated with an acute airway inflammation that appeared similar to preceding petrodiesel exposure studies. The present findings, together with the recently reported adverse cardiovascular effects after similar biodiesel exposure, indicate that biodiesel is not free of toxicity and may affect human health.

Antonio Iglesias, Fernando Mata, Joaquim Lima Cerqueira et al.

The veal niche market is gaining momentum in Brazil. Locally known as ‘Vitelão’, veal refers to the meat from calves slaughtered up to 12 months of age. In this study, we assessed the Galician Blond × Nelore cross as a candidate to produce veal. The aim of this study was to establish criteria for selecting 12-month-old calves suitable for slaughter. To find the best fit, we adjusted various growth models for calves up to 12 months of age. Once the best fit was determined, the selected growth model was then used to calculate the relative and instantaneous growth rates to evaluate the slaughtering potential at 12 months. Our study reveals that, under present conditions, the Logistic model is the best fit for characterizing and functionally analyzing growth from birth to 12 months of age in Galician Blond crosses with Nelore. Calves resulting from this cross experience rapid growth in their first 12 months of life, making them an excellent choice for producing high-quality veal while maintaining rusticity and adaptability to extreme environments. The results of this study could contribute to enhancing the growth management systems of Galician Blond and Nelore crosses in Brazilian grazing production systems. Additionally, they can be incorporated into genetic improvement programs as a tool for selecting animals with greater precocious growth without altering adult weight.

Angela Tesalonika Oktavera, Innaha Ilma Wardaya

Introduction: The hospital is one of workplaces that has a high risk of hazard that can cause near miss. Nurses are people who play an essential role in the medical service environment. Near miss are more common than work accidents and have become a critical point for preventing workplace accidents. This study aimed to analyze the correlation between compliance of PPE usage and housekeeping with near miss in nurses. Methods: This study used a descriptive observational design which was designed in a cross sectional way. Respondents were 53 nurses. The research data presented in the form of frequency distribution, cross tabulation, and correlation analysis. The study's independent variable is compliance of PPE usage and housekeeping, and the dependent variable is near miss. The method of data collecting in this research using a questionnaire for compliance of PPE usage, housekeeping, and near miss. Results: The relationship between housekeeping (rs =-0.259) with near miss among nurses is weak because near miss are primarily due to human error or unsafe behaviour, and housekeeping is a working condition factor that contributes little to the causes of near miss. Medium category correlation between compliance with PPE use (rs = -0.455) with near miss because nurses have an obligation to provide safe nursing actions and protect themselves from potential hazards by using PPE. Conclusion: Compliance of PPE usage and housekeeping that contribute to near miss.

Takumi Umemura, Yoshikazu Mutoh, Makiko Sukawa et al.

In 2016, Tosei General Hospital, a tertiary emergency medical facility with 633 beds in Japan, experienced a large nosocomial outbreak of carbapenemase-producing Enterobacterales (CPE) that had spread to numerous sink outlets. Based on our experience with using steam cleaners to suppress CPE on environmental surfaces, we report the efficacy of steam cleaners in the disinfection of sink outlets. Steam cleaners were used to disinfect 22 target areas. CPE disappeared in 90.9% of the sink outlets within the first two months after disinfection, and, after 12 months, 54.5% of the sink outlets remained negative throughout the remainder of the study period. This study demonstrates the effectiveness of using steam cleaners to disinfect sink outlets contaminated with CPE.

Marija Ćurlin, Rinea Barbir, Sanja Dabelić et al.

Abstract Background Silver nanoparticles (AgNPs) are widely used in biomedicine due to their strong antimicrobial, antifungal, and antiviral activities. Concerns about their possible negative impacts on human and environmental health directed many researchers towards the assessment of the safety and toxicity of AgNPs in both in vitro and in vivo settings. A growing body of scientific information confirms that the biodistribution of AgNPs and their toxic effects vary depending on the particle size, coating, and dose as well as on the route of administration and duration of exposure. This study aimed to clarify the sex-related differences in the outcomes of oral 28 days repeated dose exposure to AgNPs. Methods Wistar rats of both sexes were gavaged daily using low doses (0.1 and 1 mg Ag/kg b.w.) of polyvinylpyrrolidone (PVP)-coated small-sized (10 nm) AgNPs. After exposure, blood and organs of all rats were analysed through biodistribution and accumulation of Ag, whereas the state of the liver and kidneys was evaluated by the levels of reactive oxygen species (ROS) and glutathione (GSH), catalase (CAT) activity, superoxide dismutase (SOD) and glutathione peroxidase (GPx), expression of metallothionein (Mt) genes and levels of Mt proteins. Results In all animals, changes in oxidative stress markers and blood parameters were observed indicating the toxicity of AgNPs applied orally even at low doses. Sex-related differences were noticed in all assessed parameters. While female rats eliminated AgNPs from the liver and kidneys more efficiently than males when treated with low doses, the opposite was observed for animals treated with higher doses of AgNPs. Female Wistar rats exposed to 1 mg PVP-coated AgNPs/kg b.w. accumulated two to three times more silver in the blood, liver, kidney and hearth than males, while the accumulation in most organs of digestive tract was more than ten times higher compared to males. Oxidative stress responses in the organs of males, except the liver of males treated with high doses, were less intense than in the organs of females. However, both Mt genes and Mt protein expression were significantly reduced after treatment in the liver and kidneys of males, while they remained unchanged in females. Conclusions Observed toxicity effects of AgNPs in Wistar rats revealed sex-related differences in response to an oral 28 days repeated exposure.

Luga Rizqi Cristenzein, Kadek Tresna Adhi

Introduction: Study related to work stress is usually more focused on the industrial sector. Meanwhile, workers in other sectors such as the government organization also have the potential to feel stressed due to their job. During the COVID-19 (Coronavirus Disease) pandemic, Health Office is one of the main stakeholders in handling and preventing COVID-19. The COVID-19 pandemic can cause work stress due to unachieved health programs and huge demands to develop programs related to this pandemic. This study analyzed the relationship between individual factors, work factors, and factors outside of work with level of work stress among Health Office employees. This study is expected to be able to analyze work stress and its determinant as early as possible. Methods: This study was a cross-sectional study using the Depression, Anxiety, Stress Scale 42 (DASS-42) and NIOSH (National Institute for Occupational Safety and Health) Generic Job Stress Questionnaire instrument. This study was conducted at the Public Health Office Bogor Regency April 2021. This study used total sampling method on employees of the Public Health Office Bogor Regency with total sample collected of 135 respondents. Data analysis in this study was performed using multiple logistic regression. Results: 86.67% of respondents did not experience work stress, 5.93% of respondents had mild work stress, and 7.41% of respondents experienced moderate work stress. Moreover, workload (p = 0.0001) and social support (p = 0.011) had a significant relationship in increasing work stress. Conclusion: Workload was the most dominant variable affecting work stress in which workers who had high subjective workload were 33.63 times more stressful compared to workers who had the appropriate workload. Prevention of occupational stress can be done by adjusting workloads and building a good social environment between colleagues. Keywords: factors outside of work, health office, individual factors, work factors, work stress

S. E. Okhrimenko, I. Korenkov, N. I. Prokhorov et al.

Introduction. Currently, modern medical radiation technologies, including the use of radiopharmaceuticals, are becoming more widely used. At the same time, it should be noted that these technologies themselves have undergone a significant evolution towards improving their radiation safety. But while radiation technologies have been steadily improving, approaches to controlling and regulating activities with ionizing radiation sources remain highly conservative. In law enforcement practice, this approach leads more and more to excessive requirements that are not justified for the basic principles of radiation safety - rationing, justification, optimization. This paper substantiates the ways to improve the issues of regulation and regulation of activities using modern radiation technologies Purpose of research. Conducting radiation and hygiene assessment of working conditions in PET centers and developing proposals for improving regulatory requirements, taking into account the features of modern technologies Material and methods. Protocols of industrial radiation control, results of own research of effective equivalent doses, equivalent doses for the skin and lens of the eye by thermoluminescent dosimetry, timekeeping of the working time of the PET center staff were used. Results. As part of the work, the results of industrial radiation monitoring of the PET- center of a large medical organization were analyzed. It is shown that the annual radiation doses are within no more than 1/3 of the dose limit for limited personnel, most of whom receive less than 5 mS per year. The data of our research carried out based on the scientific LRC of the Department of radiochemistry of the Lomonosov Moscow State University are in good agreement with the data of the medical institution. Conducting time-lapse studies allowed us to conclude that the time of direct exposure of personnel is significantly less than the standard values provided for by regulatory documents, and the dose rates for many operations can not correspond to the values regulated by these documents. It is shown how a safe technology can relate, on formal grounds, to “highly dangerous”, which is a significant modern contradiction in the field of regulating activities with radiation sources. Conclusion. An important factor in ensuring radiation safety is the identification of critical groups of personnel, the most exposed organs, and tissues, taking into account the annual activity consumed. The standardized dose rate criteria laid down in regulatory documents do not provide for the use of indicators of the actual workload (time of exposure). It is necessary to introduce appropriate changes to the normative documents.

Wolfgang G. Kreyling, Uwe Holzwarth, Stephanie Hirn et al.

Abstract Background There is a steadily increasing quantity of silver nanoparticles (AgNP) produced for numerous industrial, medicinal and private purposes, leading to an increased risk of inhalation exposure for both professionals and consumers. Particle inhalation can result in inflammatory and allergic responses, and there are concerns about other negative health effects from either acute or chronic low-dose exposure. Results To study the fate of inhaled AgNP, healthy adult rats were exposed to 1½-hour intra-tracheal inhalations of pristine 105Ag-radiolabeled, 20 nm AgNP aerosols (with mean doses across all rats of each exposure group of deposited NP-mass and NP-number being 13.5 ± 3.6 μg, 7.9 ± 3.2•1011, respectively). At five time-points (0.75 h, 4 h, 24 h, 7d, 28d) post-exposure (p.e.), a complete balance of the [105Ag]AgNP fate and its degradation products were quantified in organs, tissues, carcass, lavage and body fluids, including excretions. Rapid dissolution of [105Ag]Ag-ions from the [105Ag]AgNP surface was apparent together with both fast particulate airway clearance and long-term particulate clearance from the alveolar region to the larynx. The results are compatible with evidence from the literature that the released [105Ag]Ag-ions precipitate rapidly to low-solubility [105Ag]Ag-salts in the ion-rich epithelial lining lung fluid (ELF) and blood. Based on the existing literature, the degradation products rapidly translocate across the air-blood-barrier (ABB) into the blood and are eliminated via the liver and gall-bladder into the small intestine for fecal excretion. The pathway of [105Ag]Ag-salt precipitates was compatible with auxiliary biokinetics studies at 24 h and 7 days after either intravenous injection or intratracheal or oral instillation of [110mAg]AgNO3 solutions in sentinel groups of rats. However, dissolution of [105Ag]Ag-ions appeared not to be complete after a few hours or days but continued over two weeks p.e. This was due to the additional formation of salt layers on the [105Ag]AgNP surface that mediate and prolonge the dissolution process. The concurrent clearance of persistent cores of [105Ag]AgNP and [105Ag]Ag-salt precipitates results in the elimination of a fraction > 0.8 (per ILD) after one week, each particulate Ag-species accounting for about half of this. After 28 days p.e. the cleared fraction rises marginally to 0.94 while 2/3 of the remaining [105Ag]AgNP are retained in the lungs and 1/3 in secondary organs and tissues with an unknown partition of the Ag species involved. However, making use of our previous biokinetics studies of poorly soluble [195Au]AuNP of the same size and under identical experimental and exposure conditions (Kreyling et al., ACS Nano 2018), the kinetics of the ABB-translocation of [105Ag]Ag-salt precipitates was estimated to reach a fractional maximum of 0.12 at day 3 p.e. and became undetectable 16 days p.e. Hence, persistent cores of [105Ag]AgNP were cleared throughout the study period. Urinary [105Ag]Ag excretion is minimal, finally accumulating to 0.016. Conclusion The biokinetics of inhaled [105Ag]AgNP is relatively complex since the dissolving [105Ag]Ag-ions (a) form salt layers on the [105Ag]AgNP surface which retard dissolution and (b) the [105Ag]Ag-ions released from the [105Ag]AgNP surface form poorly-soluble precipitates of [105Ag]Ag-salts in ELF. Therefore, hardly any [105Ag]Ag-ion clearance occurs from the lungs but instead [105Ag]AgNP and nano-sized precipitated [105Ag]Ag-salt are cleared via the larynx into GIT and, in addition, via blood, liver, gall bladder into GIT with one common excretional pathway via feces out of the body.

Franklin Jesús Pacheco Coello

El xileno o xilol es un disolvente orgánico usado en diversas áreas industriales y juega un papel fundamental en la anatomía patológica, ya que es un buen disolvente de la parafina. El objetivo de esta investigación fue determinar los niveles de ácido metilhipúrico en orina, como biomarcador de exposición ocupacional a xileno, y las condiciones laborales de estos trabajadores. Se realizó un estudio observacional, descriptivo, de correlación y transversal de campo en el que participaron trabajadores con exposición ocupacional a xileno y sin exposición, previo consentimiento informado. Se empleó el método niosh 8301 para ácido metilhipúrico y el de Jaffé modificado para la determinación de creatinina, en muestras de orina obtenidas en las últimas 4 horas del último día de su jornada laboral semanal. Se observó diferencia estadísticamente significativa (p<0,05), en los niveles de ácidos metilhipúricos en orina entre ambos grupos. El 14,4 % de los trabajadores expuestos presentó valores de ácido metilhipúrico por encima del índice biológico de exposición (bei: hasta 1 g/g de creatinina). Los niveles de ácidos metilhipúricos tienden a aumentar conforme la antigüedad laboral (r=0,7019). Este estudio representa un indicio de que se requieren evaluaciones periódicas de biomarcadores indirectos de exposición a xileno que muestren los daños a la salud de este personal.

Fauziah Mukti Sugiharto

ABSTRACT Introduction: Aircraft maintenance officer is one of the professions which is prone to work stress, because it requires abundant amount of knowledge and skills, as well as rapidity and accuracy in completing the task. Moreover, poor work environments such as noise, vibration, and extreme temperature also add to more workload and trigger occupational stress. This research aims to identify the relationship between mental workload and occupational stress on the aircraft maintenance officers at PT. X. Metods: This research was a descriptive observational research with cross-sectional design. The sample in this research was the total population of officers at PT. X, comprising 21 engineers and 20 mechanics. The total of 41 officers was used as the respondents in this research. The data of mental workload was collected using NASA-TLX method and Brief Job Stress Questionnaire was used to measure occupational stress among aircraft maintenance officers at PT. X. Results: The results of this study demonstrated that most officers had a heavy level of mental workload and experienced a moderate level of occupational stress. The Spearman-rho correlation test showed that there was a moderate relationship between mental workload and occupational stress (r = 0.306). Conclusion: It can be concluded that there was a relationship between mental workload and occupational stress among aircraft maintenance officers at PT. X.   Keywords: aircraft maintenance officer, mental workload, occupational stress

Sarah Robertson, Mark R. Miller

Abstract Air pollution is a growing public health concern of global significance. Acute and chronic exposure is known to impair cardiovascular function, exacerbate disease and increase cardiovascular mortality. Several plausible biological mechanisms have been proposed for these associations, however, at present, the pathways are incomplete. A seminal review by the American Heart Association (2010) concluded that the thrombotic effects of particulate air pollution likely contributed to their effects on cardiovascular mortality and morbidity. The aim of the current review is to appraise the newly accumulated scientific evidence (2009–2016) on contribution of haemostasis and thrombosis towards cardiovascular disease induced by exposure to both particulate and gaseous pollutants. Seventy four publications were reviewed in-depth. The weight of evidence suggests that acute exposure to fine particulate matter (PM2.5) induces a shift in the haemostatic balance towards a pro-thrombotic/pro-coagulative state. Insufficient data was available to ascertain if a similar relationship exists for gaseous pollutants, and very few studies have addressed long-term exposure to ambient air pollution. Platelet activation, oxidative stress, interplay between interleukin-6 and tissue factor, all appear to be potentially important mechanisms in pollution-mediated thrombosis, together with an emerging role for circulating microvesicles and epigenetic changes. Overall, the recent literature supports, and arguably strengthens, the contention that air pollution contributes to cardiovascular morbidity by promoting haemostasis. The volume and diversity of the evidence highlights the complexity of the pathophysiologic mechanisms by which air pollution promotes thrombosis; multiple pathways are plausible and it is most likely they act in concert. Future research should address the role gaseous pollutants play in the cardiovascular effects of air pollution mixture and direct comparison of potentially susceptible groups to healthy individuals.

Tria Melissa, Endang Dwiyanti

Fatigue is a subjective feeling that everyone feels. Work fatigue may occur due to various factors such as age, years of working life, nutritional status, and noise. The purpose of this study was to know the relation between the factors cause work fatigue at fish feed production machine operator. This research was descriptive observational with cross-sectional design. Population in this research is all worker machine operator of fish feed production who worked in the same shift at the time of the research. Samples were taken with a total sampling principle that all operator’s workers from production area who totaled 24 people. The variables were age, years of working life, nutritional status, noise, and subjective work fatigue. The results showed that most of the respondents were over 40 years old, had a working period over 10 years, had normal nutritional status, worked in the work area exceeding the noise treshold limit value and had moderate fatigue. The conclusions of this study are age, years of working life, and nutritional status has no relationship causing fatigue. Noise can be one of the factor of the occurrence of work fatigue because (54.2%) workers who work in the work area exceeding the standardize had a moderate fatigue. Keywords: noise, nutritional status, worker, work fatigue

H. Hirdi

Dora Inés Munevar M.

Mientras escucho las múltiples voces de mujeres y de hombres que permanecen en el primer piso del edificio 471, escucho el recrear sonoro del viento agitando las copas de los árboles que diviso por la ventana de la oficina 514. Con este doble telón académico de fondo, comienzo la lectura reflexiva de la versión digital de cinco capítulos estrechamente relacionados entre sí pues constituyen un conjunto de posibilidades significativas acerca de las ocupaciones en acción, lo mismo que de los seres ocupacionales en concreto.

Paula Milenka Cancino González, Alejandra Karina Reinoso Aguiló, Constanza Edith Rojas Rodríguez

Introduction: The origin of the disability is not only in the individual. It is on the interaction between individual and contextual factors and these last ones determine opportunities of participation. When factors are varying, they can modify the condition of disability and eliminate it, even if the person maintains his diagnosis of health. In this sense, public policies are important to address the issue. Thisresearch will focus on the Chilean Cultural Policy,which establishes among its principles:the promotion of access and participation of the community in artistic and cultural initiatives. It is included the variable disability, undoubtedly. Objetive: The objective of the research is to contribute to the effective and appropriate link between the variables “disability” and “access to culture” from public policies, exploring cultural management challenges. Methodology: Based on the concept of universal accessibility, this research has a Methodology of qualitative type. It analyses the public policy programs associated with the variable disability of three State services: Directorate of Libraries, Archives and Museums, National Disability Service and National Council of Culture and Arts. Results:It is evident from the analysis that references of disability and culture are integrated by the Chilean State, and it should certainly be recognized as an advance, but these references do not guarantee the optimum universal accessibility. Conclusions: The cultural manager appears as a relevant actor in the formulation of cultural policy and therefore in the construction of the disability situation.

Estelle Durantie, Dimitri Vanhecke, Laura Rodriguez-Lorenzo et al.

Abstract Background The lung represents the primary entry route for airborne particles into the human body. Most studies addressed possible adverse effects using single (nano)particles, but aerosolic nanoparticles (NPs) tend to aggregate and form structures of several hundreds nm in diameter, changing the physico-chemical properties and interaction with cells. Our aim was to investigate how aggregation might affect the biodistribution; cellular uptake and translocation over time of aerosolized NPs at the air-blood barrier interface using a multicellular lung system. Results Model gold nanoparticles (AuNPs) were engineered and well characterized to compare single NPs with aggregated NPs with hydrodynamic diameter of 32 and 106 nm, respectively. Exposures were performed by aerosolization of the particles onto the air-liquid interface of a three dimensional (3D) lung model. Particle deposition, cellular uptake and translocation kinetics of single and aggregated AuNPs were determined for various concentrations, (30, 60, 150 and 300 ng/cm2) and time points (4, 24 and 48 h) using transmission electron microscopy and inductively coupled plasma optical emission spectroscopy. No apparent harmful effect for single and aggregated AuNPs was observed by lactate dehydrogenase assay, nor pro-inflammation response by tumor necrosis factor α assessment. The cell layer integrity was also not impaired. The bio-distribution revealed that majority of the AuNPs, single or aggregated, were inside the cells, and only a minor fraction, less than 5%, was found on the basolateral side. No significant difference was observed in the translocation rate. However, aggregated AuNPs showed a significantly faster cellular uptake than single AuNPs at the first time point, i.e. 4 h. Conclusions Our studies revealed that aggregated AuNPs showed significantly faster cellular uptake than single AuNPs at the first time point, i.e. 4 h, but the uptake rate was similar at later time points. In addition, aggregation did not affect translocation rate across the lung barrier model since similar translocation rates were observed for single as well as aggregated AuNPs.

Monica Palacios Tolvett, Carmen Silva Dreyer

Actualmente en Chile, a la luz de la opinión pública, los jóvenes que han infringido la ley son asociados con la violencia, el consumo de drogas, y la delincuencia, haciéndose acreedores de una identidad social negativa, lo que profundiza su estigmatizadón y descalificación a priori. Así mismo, no existe una valoración de lo que ellos son, hacen, o del sentido de sus ocupaciones. Este estudio pretende responder: ¿Cuáles son los significados que los/las jóvenes infractores de ley dan a ocupaciones que realizan en su vida cotidiana?; ¿muestran estas ocupaciones atributos distintos a la infracción de la ley? Para ello se utilizó una metodología cualitativa, basada en la teoría fundada, mediante entrevistas semi-estructuradas, ocho individuales y dos grupales, efectuando codificación abierta, descripción y análisis detallado de significados. Los participantes fueron hombres y mujeres entre los 14 y los 25 años de edad, infractores de ley, pertenecientes a comunas de la Región Metropolitana, quienes hacían parte de programas de inclusión social. Los resultados muestran que los jóvenes realizan diversas ocupaciones más allá de solo la infracción de ley. Aparecen dos ejes: uno de ocupaciones aceptadas y otro de ocupaciones transgresoras, los significados positivos o negativos, para cada eje, configuran identidad y posibilidad de cambio.

M. Friesen, Q. Lan, C. Ge et al.