Hasil untuk "Engineering"

M. Sanders, E. J. Mccormick

W. Ames

H. Jasik

G. Stephanopoulos

E. Kranakis, E. Ferguson

Asunción Gómez-Pérez, Óscar Corcho, M. Fernández-López

J. Keasling

N. Jennings

S. Atsumi, A. Cann, Michael R. Connor et al.

Prof. Kwang-Chun, K. Ho

G. Muschler, Chizu Nakamoto, L. Griffith

Reshma P. Shetty, Drew Endy, Thomas F. Knight

BackgroundThe underlying goal of synthetic biology is to make the process of engineering biological systems easier. Recent work has focused on defining and developing standard biological parts. The technical standard that has gained the most traction in the synthetic biology community is the BioBrick standard for physical composition of genetic parts. Parts that conform to the BioBrick assembly standard are BioBrick standard biological parts. To date, over 2,000 BioBrick parts have been contributed to, and are available from, the Registry of Standard Biological Parts.ResultsHere we extended the same advantages of BioBrick standard biological parts to the plasmid-based vectors that are used to provide and propagate BioBrick parts. We developed a process for engineering BioBrick vectors from BioBrick parts. We designed a new set of BioBrick parts that encode many useful vector functions. We combined the new parts to make a BioBrick base vector that facilitates BioBrick vector construction. We demonstrated the utility of the process by constructing seven new BioBrick vectors. We also successfully used the resulting vectors to assemble and propagate other BioBrick standard biological parts.ConclusionWe extended the principles of part reuse and standardization to BioBrick vectors. As a result, myriad new BioBrick vectors can be readily produced from all existing and newly designed BioBrick parts. We invite the synthetic biology community to (1) use the process to make and share new BioBrick vectors; (2) expand the current collection of BioBrick vector parts; and (3) characterize and improve the available collection of BioBrick vector parts.

R. Sinnott

F. V. Linden, Klaus Schmid, E. Rommes

L. Ghasemi‐Mobarakeh, M. Prabhakaran, M. Morshed et al.

Among the numerous attempts to integrate tissue engineering concepts into strategies to repair nearly all parts of the body, neuronal repair stands out. This is partially due to the complexity of the nervous anatomical system, its functioning and the inefficiency of conventional repair approaches, which are based on single components of either biomaterials or cells alone. Electrical stimulation has been shown to enhance the nerve regeneration process and this consequently makes the use of electrically conductive polymers very attractive for the construction of scaffolds for nerve tissue engineering. In this review, by taking into consideration the electrical properties of nerve cells and the effect of electrical stimulation on nerve cells, we discuss the most commonly utilized conductive polymers, polypyrrole (PPy) and polyaniline (PANI), along with their design and modifications, thus making them suitable scaffolds for nerve tissue engineering. Other electrospun, composite, conductive scaffolds, such as PANI/gelatin and PPy/poly(ε‐caprolactone), with or without electrical stimulation, are also discussed. Different procedures of electrical stimulation which have been used in tissue engineering, with examples on their specific applications in tissue engineering, are also discussed. Copyright © 2011 John Wiley & Sons, Ltd.

F. Berthiaume, T. Maguire, M. Yarmush

J. Herbsleb

Yuriy Brun, G. Serugendo, C. Gacek et al.

Qizhi Chen, Shuling Liang, G. Thouas

Wan-Gu Kim, Byoung-Nam Kim, Yohan Chweh

The influence of station-to-station line orientation on sea current speed observations using Coastal Acoustic Tomography (CAT) was quantitatively investigated. For this purpose, we conducted CAT experiments at five stations in Yeosu Bay, South Korea. Through these experiments, the sea current speeds were estimated along a total of six tomographic observation lines with different orientations, and the results were compared with current speeds measured simultaneously by an Acoustic Doppler Current Profiler (ADCP). The comparison showed that the concordance between tomography-estimated sea current speed and ADCP-measured sea current speed tended to decrease as the acute angle between the predominant tidal current direction in Yeosu Bay and a tomographic observation line increased. This tendency is interpreted as arising because the smaller the difference between the two one-way travel times obtained during tomographic observations, the greater the effect of the travel time measurement error whose magnitude is relatively direction-independent. This interpretation was supported by a simple numerical simulation. Furthermore, quantitative analysis of these simulation results indicated that a smaller acute angle between the predominant sea current direction in the survey area and a tomographic observation line enhances the robustness of sea current speed estimation against travel time measurement errors. The results show that the station-to-station line in CAT should be arranged considering the predominant sea current direction in the survey area, which can provide an important guideline for selecting station locations.