Hasil untuk "Veterinary medicine"

B. C. Sutton

A. Nikolaou, S. Meriç, D. Fatta

Aben Ovung, Jhimli Bhattacharyya

N. Suttle

The fourth edition of this important book covers new advances in livestock mineral nutrition, updated with more illustrations and additional material on the relationship between livestock and man. Recent developments are discussed, such as increasing the mineral value of feeds by the use of additives and enhancing mineral availability through the use of organic sources of trace elements. The concept of the mineral footprint of livestock production is introduced and methods of mineral feeding that lower environmental pollution are presented. Opportunities and problems in manipulating the mineral content of livestock to improve the mineral status of consumers are also addressed. The book is an essential resource for researchers and students in animal nutrition, agriculture and veterinary medicine, and a useful reference for those concerned with human nutrition and environmental protection.

M. Acierno, S. Brown, A. Coleman et al.

An update to the 2007 American College of Veterinary Internal Medicine (ACVIM) consensus statement on the identification, evaluation, and management of systemic hypertension in dogs and cats was presented at the 2017 ACVIM Forum in National Harbor, MD. The updated consensus statement is presented here. The consensus statement aims to provide guidance on appropriate diagnosis and treatment of hypertension in dogs and cats.

W. Baran, E. Adamek, J. Ziemiańska et al.

D. Plumb

R. Laing, Victoria Gillan, E. Devaney

Ivermectin is one of the most important drugs in veterinary and human medicine for the control of parasitic infection and was the joint focus of the 2015 Nobel Prize in Physiology or Medicine, some 35 years after its remarkable discovery. Although best described for its activity on glutamate-gated chloride channels in parasitic nematodes, understanding of its mode of action remains incomplete. In the field of veterinary medicine, resistance to ivermectin is now widespread, but the mechanisms underlying resistance are unresolved. Here we discuss the history of this versatile drug and its use in global health. Based on recent studies in a variety of systems, we question whether ivermectin could have additional modes of action on parasitic nematodes.

J. Verstegen, M. Iguer-ouada, K. Onclin

C. Pomba, M. Rantala, C. Greko et al.

G. Segev, S. Cortellini, J. D. Foster et al.

Acute kidney injury (AKI) is defined as an injury to the renal parenchyma, with or without a decrease in kidney function, as reflected by accumulation of uremic toxins or altered urine production (i.e., increased or decreased). AKI might result from any of several factors, including ischemia, inflammation, nephrotoxins, and infectious diseases. AKI can be community- or hospital-acquired. The latter was not previously considered a common cause for AKI in animals; however, recent evidence suggests that the prevalence of hospital-acquired AKI is increasing in veterinary medicine. This is likely due to a combination of increased recognition and awareness of AKI, as well as increased treatment intensity (e.g., ventilation and prolonged hospitalization) in some veterinary patients and increased management of geriatric veterinary patients with multiple comorbidities. Advancements in the management of AKI, including the increased availability of renal replacement therapies, have been made; however, the overall mortality of AKI in animals remains high. Despite the high prevalence of AKI and the high mortality rate, the body of evidence regarding the diagnosis and the management of AKI in veterinary medicine is very limited. Consequently, the International Renal Interest Society (IRIS) constructed a working group to provide guidelines for animals with AKI. Recommendations are based on the available literature and the clinical experience of the members of the working group and reflect consensus of opinion. Fifty statements were generated and were voted on in all aspects of AKI and explanatory text can be found either before or after each statement.

Joseph D’Orazio, Lewis S. Nelson, Jeanmarie Perrone et al.

Xylazine is an animal sedative, approved by the U.S. Food and Drug Administration, that is commonly used in veterinary medicine and is not approved for human use. Since 2016, xylazine has consistently appeared in the illicitly manufactured fentanyl supply and has significantly increased in prevalence, likely due to its low cost, easy availability, and presumed synergistic psychoactive effect. Clinical experience along with the available pertinent research were used to review xylazine adulteration of the drug supply and provide guidance on the care of patients exposed to xylazine. This review discusses xylazine pharmacology, animal and human clinical effects, and what is known to date about care of patients experiencing acute overdose, xylazine-fentanyl withdrawal, and xylazine-associated wounds.

Abraham Akinyemi, Olumide Ajani, Olumide Akinniyi

Monosodium glutamate (MSG) is a widespread flavour enhancer linked to health risks, including male reproductive dysfunction. This study investigated tiger nut (Cyperus esculentus) as a potential protective agent against MSG-induced reproductive issues in male Wistar rats. Forty adult rats were divided into four groups: control, MSG-only (2 mg/g), tiger nut-only (500 mg/kg), and MSG+tiger nut combination (2 mg/g MSG + 500 mg/kg tiger nut). Treatments were administered orally for 28 days, with analyses conducted at days 14 and 28. Results showed significant variations in sperm parameters. At 14 days, the tiger nut group showed highest sperm motility (88.60±4.04%) and count (100.60±3.21×106/mL), while MSG reduced sperm viability (70.00±4.69%). By 28 days, MSG significantly decreased sperm motility (41.80±4.92%) and viability (54.80±6.76%). MSG increased sperm abnormalities at 14 days (13.60±2.51%) but normalized by 28 days. The MSG+tiger nut combination eliminated certain sperm abnormalities like coiled tail and tail-without-head. Gonadometric parameters remained stable throughout the study, indicating tiger nut's ability to maintain testicular architecture despite MSG exposure. Initial body weight increases in the MSG group normalized by weeks 3-4. The study concludes that tiger nut juice significantly protects against MSG-induced low sperm quality in male Wistar rats, suggesting its potential as a protective supplement for populations with unavoidable MSG exposure. Future research should explore long-term effects and cellular mechanisms.

Chenxi Li, Chenyang Tang, Xiqian Liu et al.

ABSTRACT Membrane-associated RING-CH-type finger (MARCH) proteins, a class of E3 ubiquitin ligases, have been reported to be involved in the infection of multiple viruses and the regulation of type I interferon (IFN) production. However, the specific role and mechanisms by which MARCH proteins influence Japanese encephalitis virus (JEV) infection remain poorly understood. Here, we systematically investigate the functional relevance of MARCH proteins in JEV replication by examining the effects of siRNA-mediated knockdown of MARCHs on viral infection. We identified MARCH5 as a positive regulator of JEV replication. The knockout of MARCH5 dramatically reduced viral yields, whereas its overexpression significantly enhanced JEV replication. Mechanistically, MARCH5 specifically interacts with the JEV envelope (E) protein and promotes its K27-linked polyubiquitination at the lysine (K) residues 136 and 166. This ubiquitination enhances viral attachment to permissive cells. Substituting these lysine residues with arginine (R) attenuated JEV replication in vitro and reduced viral virulence in vivo. Furthermore, JEV infection upregulated the expression of MARCH5. We also discovered that MARCH5 degrades mitochondrial antiviral-signaling protein (MAVS) through the ubiquitin-proteasome pathway by catalyzing its K48-linked ubiquitination, thereby inhibiting type I IFN production in JEV-infected cells. This suppression of type I IFN further facilitates JEV infection. In conclusion, these findings disclosed a novel role of MARCH5 in positively regulating JEV infection and revealed an important mechanism employed by MARCH5 to regulate the innate immune response.IMPORTANCEJEV is the leading cause of viral encephalitis in many countries of Asia with an estimated 100,000 clinical human cases and causes economic loss to the swine industry. Until now, there is no clinically approved antiviral for the treatment of JEV infection. Although vaccination prophylaxis is widely regarded as the most effective strategy for preventing Japanese encephalitis (JE), the incidence of JE cases continues to rise. Thus, a deeper understanding of virus-host interaction will enrich our knowledge of the mechanisms underlying JEV infection and identify novel targets for the development of next-generation live-attenuated vaccines and antiviral therapies. To the best of our knowledge, this study is the first to identify MARCH5 as a pro-viral host factor that facilitates JEV infection. We elucidated two distinct mechanisms by which MARCH5 promotes JEV infection. First, MARCH5 interacts with viral E protein and mediates the K27-linked ubiquitination of E protein at the K136 and K166 residues to facilitate efficient viral attachment. Furthermore, double mutations of K136R-K166R attenuated JEV infection in vitro and reduced viral virulence in mice. Second, the upregulated expression of MARCH5 induced by JEV infection further suppresses the RIG-I-like receptor (RLR) signaling pathway to benefit viral infection. MARCH5 downregulates type I IFN production by conjugating the K48-linked polyubiquitin at the K286 of MAVS, which leads to MAVS degradation through the ubiquitin-proteasome pathway. In summary, this study provides novel insights into the role played by MARCH proteins in JEV infection and identifies specific ubiquitination sites on JEV E protein that could be targeted for viral attenuation and the development of antiviral therapeutics.

S. Cortellini, A. E. DeClue, M. Giunti et al.

OBJECTIVE To discuss the definitions of sepsis in human and veterinary medicine. DESIGN International, multicenter position statement on the need for consensus definitions of sepsis in veterinary medicine. SETTING Veterinary private practice and university teaching hospitals. ANIMALS Dogs and cats. INTERVENTIONS None. MEASUREMENTS AND MAIN RESULTS Sepsis is a life-threatening condition associated with the body's response to an infection. In human medicine, sepsis has been defined by consensus on 3 occasions, most recently in 2016. In veterinary medicine, there is little uniformity in how sepsis is defined and no consensus on how to identify it clinically. Most publications rely on modified criteria derived from the 1991 and 2001 human consensus definitions. There is a divergence between the human and veterinary descriptions of sepsis and no consensus on how to diagnose the syndrome. This impedes research, hampers the translation of pathophysiology insights to the clinic, and limits our abilities to optimize patient care. It may be time to formally define sepsis in veterinary medicine to help the field move forward. In this narrative review, we present a synopsis of prior attempts to define sepsis in human and veterinary medicine, discuss developments in our understanding, and highlight some criticisms and shortcomings of existing schemes. CONCLUSIONS This review is intended to serve as the foundation of current efforts to establish a consensus definition for sepsis in small animals and ultimately generate evidence-based criteria for its recognition in veterinary clinical practice.

Floriana-Bogdana CIOBANU, Marius VLADU

The LEADER programme provides new opportunities for rural development, identifies the local gaps/needs and implements the new solutions using a bottom-up approach. The publication follows the evolution of the programme both at European and national level, namely in Romania. The information was generated by the Ministry of Agriculture website and Rural Development and by the European Commission. They were processed by the Court of Accounts. In the European Union it was first introduced 32 years ago while in Romania only 16 years ago. The central mechanism and at the same time the beneficiary of this programme is the LAG (Local Action Group), meant to facilitate implementing the LEADER principles. The programme became compulsory to every member state between 2014–2020 and each state had to provide 5% of the rural development fund for the LEADER policy, excepting Croatia since it had just adhered to the EU, in 2013.In Romania, between 2011–2012, 163 LAGs were established while currently the number goes up to 259. However, parts of the Romanian territory are still not covered by any LAG, although they are eligible for the LEADER implementation. On the other hand, between 2014–2020 some LAGs’ functioning authorizations were canceled. In Europe, a number of 143,487 LEADER projects were registered. Although the EU planned to provide funding of up to Euro 9.2 billion, the real amount was of 7 billion.

Laura Torres Borda, Peter Roth, Jennifer Lumetzberger et al.

Abstract Sociopositive interactions with conspecifics are essential for equine welfare and quality of life. This study aimed to validate the use of wearable ultra-wideband (UWB) technology to quantify the spatial relationships and dynamics of social behaviour in horses by continuous (1/s) measurement of interindividual distances. After testing the UWB devices’ spatiotemporal accuracy in a static environment, the UWB measurement validity, feasibility and utility under dynamic field conditions was assessed in a group of 8 horses. Comparison of the proximity measurements with video surveillance data established the measurement accuracy and validity (r = 0.83, p < 0.0001) of the UWB technology. The utility for social behaviour research was demonstrated by the excellent accordance of affiliative relationships (preferred partners) identified using UWB with video observations. The horses remained a median of 5.82 m (95% CI 5.13–6.41 m) apart from each other and spent 20% (median, 95% CI 14–26%) of their time in a distance ≤ 3 m to their preferred partner. The proximity measurements and corresponding speed calculation allowed the identification of affiliative versus agonistic approaches based on differences in the approach speed and the distance and duration of the resulting proximity. Affiliative approaches were statistically significantly slower (median: 1.57 km/h, 95% CI 1.26–1.92 km/h, p = 0.0394) and resulted in greater proximity (median: 36.75 cm, 95% CI 19.5–62 cm, p = 0.0003) to the approached horse than agonistic approaches (median: 3.04 km/h, 95% CI 2.16–3.74 km/h, median proximity: 243 cm, 95% CI 130–319 cm), which caused an immediate retreat of the approached horse at a significantly greater speed (median: 3.77 km/h, 95% CI 3.52–5.85 km/h, p < 0.0001) than the approach.

Dimitrios Komodromos, Daniel Sergelidis, Ioannis Amvrosiadis et al.

In the present study, the combined effect of an AgIon^® antimicrobial absorbent (Ζ) pad and a chitosan coating (C) on the preservation of fresh beef stored aerobically at 5 °C was investigated. Microbiological, physicochemical, and sensory attributes were monitored for up to 10 days of storage. The microbiological data indicated that the C and chitosan coating plus absorbent pad (CZ) treatments were the most efficient in reducing total viable counts (TVC) by 4.09 and 3.53 log cfu/g compared to the control W and Z treatments on day 4 of storage (<i>p</i> < 0.05). An analogous reduction in the counts of the other microbial groups monitored was recorded. pH values were ca. 5.7 for treatments W and Z and 5.45 for treatments C and CZ on day 4 of storage (<i>p</i> < 0.05). The total volatile basic nitrogen (TVB-N) values remained <20 mg/100 g for all treatments on day 4 and for treatments C and CZ on day 10 of storage. The total color difference values decreased (<i>p</i> < 0.05) during storage for treatments W and Z, but remained constant for treatments C and CZ. Based on sensory, microbiological and physico-chemical data, beef shelf life was ca ^# + 3 days for samples W and Z and at least 10 + 3 days for samples C and CZ. Between the two antimicrobial treatments, chitosan was considerably more effective than the AgIon^® antimicrobial absorbent pad, which showed practically no antimicrobial activity in direct contact with beef meat.

Sk Injamamul Islam, Mohamed H. Hamad, Wanarit Jitsamai et al.

Clinostomum species, a parasitic pathogen of freshwater fish, is widely distributed and infects various host species. Recently, the pathological effect due to Clinostomum metacercarial infection was described in aquaculture in Thailand; however, the global genetic diversity and population structure of this species have not been studied yet. Therefore, this study aimed to provide a detailed description of genetic diversity and population dynamics of the digenean Clinostomum isolated from Trichopodus pectoralis with globally recorded Clinostomum species. The species was characterized molecularly by analyzing 18S rDNA and inter-transcribed spacer biomarker genes (ITS1 and ITS2). A BLAST search discovered that the 18S rDNA and ITS sequence had a 100% sequence similarity with Clinostomum piscidium isolated from India and Thailand. A comprehensive analysis revealed the presence of 12 distinct haplotypes among the Clinostomum populations. This study suggests that distinct patterns of genetic variation were identified by analyzing molecular variance, pairwise Fst, and employing structure analysis. It was observed that a gradient of genetic variation exists within continents, characterized by higher levels within different groups and lower levels of genetic differentiation. Additionally, a notable presence of mixed haplotypes was observed. The results of neutrality testing suggest that there has been a significant expansion in the populations of Clinostomum in India, America, and Kenya. The discoveries from this study will provide a valuable contribution to comprehending the genetics and evolution of Clinostomum species. Furthermore, key findings will be essential in developing efficient management approaches to prevent and control this parasite.

T. V. Marakaeva