Diaby Vandjiguiba, Koffi Pierre Valery Niaba, Kamou Kamou Richard
et al.
L objectif general de l etude a ete de rechercher les coliformes fecaux dans les eaux de puits au quartier Digboue a San Pedro. Trente echantillons d eaux de puits ont ete preleves et achemines au laboratoire pour la determination du pH et la recherche des coliformes fecaux. Ainsi, les resultats obtenus ont montre que ces echantillons sont pollues en coliformes fecaux. L observation au diagramme de PARETO a montre que 80 % de charges en coliformes fecaux a ete retrouve dans 20 % des puits analyses (E10, E12, E18 et E19). Quant au pH, il a ete legerement acide voire legerement basique pour 80 % des puits et acide pour 20 % des puits selon le diagramme de PARETO adapte a cette etude. En conclusion, la pollution de ces eaux de puits en coliformes fecaux a montre quelles sont impropres a la consommation humaine et que cela necessite des traitements prealables (ebullition et desinfection) avant consommation.
Arthur Imbert, Ouyang Wei, Adham Safieddine
et al.
Regulation of RNA abundance and localization is a key step in gene expression control. Single-molecule RNA fluorescence in situ hybridization (smFISH) is a widely used single-cell-single-molecule imaging technique enabling quantitative studies of gene expression and its regulatory mechanisms. Today, these methods are applicable at a large scale, which in turn come with a need for adequate tools for data analysis and exploration. Here, we present FISH-quant v2, a highly modular tool accessible for both experts and non-experts. Our user-friendly package allows the user to segment nuclei and cells, detect isolated RNAs, decompose dense RNA clusters, quantify RNA localization patterns and visualize these results both at the single-cell level and variations within the cell population. This tool was validated and applied on large-scale smFISH image data sets, revealing diverse subcellular RNA localization patterns and a surprisingly high degree of cell-to-cell heterogeneity.
Willemijn E. Bezemer, Marise Ph. Born, Arjen S. Leerkes
Abstract Objectives Self-reported criminal behaviour has the potential to provide clearer insights into patterns of criminality compared to using police statistics. The risk of inaccurate responses however forms a major obstacle to its validity. This study therefore examines underreporting bias of self-reported criminal behaviour among five ethnic groups and compares different methods to facilitate the creation of valid intergroup comparisons. Methods This study includes data from the Monitor on Youthful Delinquency (N = 6,218) which was connected to police suspect registrations. To identify patterns of underreporting, we compared self-reported and police recorded crime with a social desirability measure, which was adjusted to be invariant across ethnic groups. Three different methods to correct for underreporting bias were subsequently compared; partialling out the effect of social desirability, listwise deletion, and a novel technique which we named Social Desirability based Score Replacement (SDSR). Results The study reveals that police suspects with a high social desirability score display a low likelihood to self-report crime when they have an ethnic minority background, but not when they have a native Dutch background or when they have a moderate to low social desirability score. This finding points towards systematic differences in underreporting bias. Model outcomes are shown to be significantly impacted depending on the method that is used to address this issue. Conclusion Neglecting to correct underreporting-bias hinders the validity of intergroup comparisons of self-reported criminal behaviour. The inclusion of a social desirability measure is therefore recommended to help identify and correct underreporting bias, particularly through the use of SDSR.
Here we describe DAB-quant, a new system that facilitates quantitation of large numbers of scanned tissue slides stained via immunohistochemistry with 3,3′-Diaminobenzidine (DAB). The python code, instructions, license, and a link to example scans for analysis are all available at: https://github.com/sarafridov/DAB-quant
Here we describe DAB-quant, a new system that facilitates quantitation of large numbers of scanned tissue slides stained via immunohistochemistry with 3,3′-Diaminobenzidine (DAB). The python code, instructions, license, and a link to example scans for analysis are all available at: https://github.com/sarafridov/DAB-quant
In recent years, the digital polymerase chain reaction has received increasing interest as it has emerged as a tool to provide more sensitive and accurate detection of minimal residual disease. In order to start the process of data alignment, we assessed the consistency of the BCR-ABL1 quantification results of the analysis of 16 RNA samples at different levels of disease. The results were obtained by two different laboratories that relied on The Qx100/Qx200 Droplet Digital PCR System (Bio-Rad) and Quant Studio 3D dPCR System (Thermofisher) platforms. We assessed the compatibility between the estimated values by linear regression, Bland–Altman bias-plot, and Mann–Whitney nonparametric test. The results confirmed the compatibility of the measures, allowing us tocompute an ‘alignment factor’ (AF), equal to 1.41, which was further validated by a different series of experiments. We conclude that the performed measurements by the two laboratories are comparable, and also equalized through the introduction of an alignment factor.
In the recent and very enjoyable paper (Paul Strange, "Semiclassical and Quantum Analysis of a Focussing Free Particle Hermite Wavefunction", arXiv:1309.6753[quant-ph]), Professor Strange has studied a particular solution of the free particle Schroedinger equation in which the time and space dependence are not separable. After recognizing the fact that "The Schroedinger equation has an identical mathematical form to the paraxial wave equation [...]", he claims to "describe and try to gain insight into an exotic, apparently accelerating solution of the free particle Schroedinger equation that is square integrable and which also displays some unusual characteristics." It is the main aim of this short note to show that the wavefunction described by Prof. Strange is simply one particular Hermite-Gauss solution of the paraxial wave equation and even more "exotic" examples can be found by considering, e.g., Laguerre-Gauss solutions.
The interpretative principle proposed by Bub in 1211.3062v1 [quant-ph] is justified only for all practical purposes (Bell's "FAPP trap"). An alternative interpretative principle is proposed. It brings to light those features of the quantum world because of which the fundamental theoretical framework of physics is a "mere" probability calculus, and it amply justifies Bohr's insistence that quantum-mechanical observables cannot be defined without reference to the experimental conditions in which they are measured. It implies that the spatial distinctions we make cannot be intrinsic to space, that regions "of space" must be realized by macroscopic objects, that the spatiotemporal differentiation of the physical world is incomplete, that the positions of macroscopic objects (suitably defined) are definite in a nonclassical sense, and that unconditional factuality can be consistently attributed to them.
Chen (quant-ph/0611126) has recently claimed ``exponential violation of local realism by separable states", in the sense that multi-partite separable quantum states are supposed to give rise to correlations and fluctuations that violate a Bell-type inequality that Chen takes to be satisfied by local realism. However, this can not be true since all predictions (including all correlations and fluctuations) that separable quantum states give rise to have a local realistic description and thus satisfy all Bell-type inequalities, and this holds for all number of parties. Since Chen claims otherwise by presenting a new inequality, claimed to be a Bell-type one, which separable states supposedly can violate, there must be a flaw in the argumentation. I will expose this flaw, not merely for clarification of this issue, but perhaps even more importantly since it re-teaches us an old lesson John Bell taught us over 40 years ago. I will argue that this lesson provides us with a new morale especially relevant to modern research in Bell-type inequalities.
Maria Immacolata La Rotonda, Gaetano Amato, Francesco Barbato
et al.
AbstractOctanol/water partition and distribution coefficients of a set of nonsteroidal anti‐inflammatory drugs were measured. Relationships between partition data at different pH values and dissociation constants are discussed.Log k′ values were determined by reversed‐phase high‐performance liquid chromatography (HPLC) on bonded octyl and octadecylsilane supports at different pH values using various organic modifiers in the mobile phase.The log k′ values exhibited linear relationships with methanol concentration in the mobile phase. Octanol/water partition data correlated well with the extrapolated log k′ indices to zero methanol concentration (solvent regression equations) and with the corresponding Rm indices measured on octyl and octadecyl reversed phases thin layer chromatography (HPTLC). Nature of the slopes and the intercepts of the relationships is discussed.It is shown that the HPLC and HPTLC methods are useful alternatives for the determination of partition data.