Hasil untuk "Animal culture"

O. Lindvall, Z. Kokaia, A. Martínez-Serrano

M. J. Martín, A. Muotri, F. Gage et al.

A. Kaser, S. Zeissig, R. Blumberg

Bauer L. LeSavage, R. Suhar, N. Broguiere et al.

S. Gunti, A. Hoke, Kenny Vu et al.

Simple Summary Cell cultures can be carried out in three dimensions (3D). Organoids and spheroids are different 3D cell culture models that can be cultured with different techniques. These 3D cell culture units established from a patient tumor have several similarities to the original tumor tissue and possess several advantages in conducting basic and clinical cancer research. Organoids prepared from a patient tissue can be preserved in a living biobank. Testing chemo-, radio- and immuno-therapies on these organoids has the potential to predict the patient responses and these models have incredible promise for personalized medicine. This review presents different organoid models, the techniques to prepare them and recent advances in their applications. Abstract Techniques to develop three-dimensional cell culture models are rapidly expanding to bridge the gap between conventional cell culture and animal models. Organoid and spheroid cultures have distinct and overlapping purposes and differ in cellular sources and protocol for establishment. Spheroids are of lower complexity structurally but are simple and popular models for drug screening. Organoids histologically and genetically resemble the original tumor from which they were derived. Ease of generation, ability for long-term culture and cryopreservation make organoids suitable for a wide range of applications. Organoids-on-chip models combine organoid methods with powerful designing and fabrication of micro-chip technology. Organoid-chip models can emulate the dynamic microenvironment of tumor pathophysiology as well as tissue–tissue interactions. In this review, we outline different tumor spheroid and organoid models and techniques to establish them. We also discuss the recent advances and applications of tumor organoids with an emphasis on tumor modeling, drug screening, personalized medicine and immunotherapy.

M. Post

S. Bellis

D. Huh, Y. Torisawa, G. Hamilton et al.

Bárbara Pinto, Ana C. Henriques, Patrícia M. A. Silva et al.

Most cancer biologists still rely on conventional two-dimensional (2D) monolayer culture techniques to test in vitro anti-tumor drugs prior to in vivo testing. However, the vast majority of promising preclinical drugs have no or weak efficacy in real patients with tumors, thereby delaying the discovery of successful therapeutics. This is because 2D culture lacks cell–cell contacts and natural tumor microenvironment, important in tumor signaling and drug response, thereby resulting in a reduced malignant phenotype compared to the real tumor. In this sense, three-dimensional (3D) cultures of cancer cells that better recapitulate in vivo cell environments emerged as scientifically accurate and low cost cancer models for preclinical screening and testing of new drug candidates before moving to expensive and time-consuming animal models. Here, we provide a comprehensive overview of 3D tumor systems and highlight the strategies for spheroid construction and evaluation tools of targeted therapies, focusing on their applicability in cancer research. Examples of the applicability of 3D culture for the evaluation of the therapeutic efficacy of nanomedicines are discussed.

A. Jovičić, J. Mertens, S. Boeynaems et al.

T. Burnouf, D. Strunk, M. Koh et al.

M. Raff

the transplanted spleens attains the mass of one normal “The most obvious differences between different aniadult spleen, suggesting that their growth is mainly conmals are differences in size, but for some reason the trolled by factors outside the spleen (Metcalf, 1964). zoologists have paid singularly little attention to them.” Similar transplantation experiments, as well as tissue —J. B. S. Haldane, On Being the Right Size, 1927 culture experiments, indicate that most animal organs

Erica van de Waal, C. Borgeaud, A. Whiten

Mironova A.A. , Mironova L.P. , Vasilenko V.N.

Manuel Fuertes-Recuero, José L. López-Hernández, Alejandra Ramírez-Lago et al.

Chimpanzees (<i>Pan troglodytes</i>) rescued from the illegal wildlife trade often suffer from chronic, traumatic injuries that require specialized and prolonged medical treatment in wildlife rehabilitation centers. We present the case report of a two-year-old male chimpanzee admitted at the Tchimpounga Chimpanzee Rehabilitation Center in the Republic of Congo with a chronic periorbital abscess, likely caused by a machete wound sustained during the poaching of his mother. Despite receiving extended antimicrobial therapy, his condition was never fully controlled and progressed to a chronic orbital infection, causing him discomfort and producing chronic purulent discharge. Enucleation was performed under general anesthesia using ketamine and medetomidine, with surgical approach adapted to the distinctive orbital anatomy of chimpanzees. During the procedure, ligation of the optic nerve and ophthalmic vessels was required due to the confined orbital apex and extensive vascularization, ensuring adequate haemostasias and procedural safety. The chimpanzee made an uneventful postoperative recovery, resuming normal feeding and social behavior within 48 h, with complete wound healing occurring within two weeks. This case report highlights the importance of prompt surgical intervention when conservative medical management fails to resolve refractory ocular infections in chimpanzees. It also emphasizes the importance of specific anesthetic protocols, refined surgical techniques and tailored postoperative care in wildlife rehabilitation centers. Documenting and sharing detailed case reports such as this contributes to the limited veterinary literature on great ape surgery and supports evidence-based clinical decision-making to improve the welfare and treatment outcomes of rescued chimpanzees.

Zeynep Akkutay-Yoldar, Mehmet Türkay Yoldar, Yiğit Burak Akkaş et al.

Abstract Identifying viral replication within cells demands labor-intensive isolation methods, requiring specialized personnel and additional confirmatory tests. To facilitate this process, we developed an AI-powered automated system called AI Recognition of Viral CPE (AIRVIC), specifically designed to detect and classify label-free cytopathic effects (CPEs) induced by SARS-CoV-2, BAdV-1, BPIV3, BoAHV-1, and two strains of BoGHV-4 in Vero and MDBK cell lines. AIRVIC utilizes convolutional neural networks, with ResNet50 as the primary architecture, trained on 40,369 microscopy images at various magnifications. AIRVIC demonstrated strong CPE detection, achieving 100% accuracy for the BoGHV-4 DN-599 strain in MDBK cells, the highest among tested strains. In contrast, the BoGHV-4 MOVAR 33/63 strain in Vero cells showed a lower accuracy of 87.99%, the lowest among all models tested. For virus classification, a multi-class accuracy of 87.61% was achieved for bovine viruses in MDBK cells; however, it dropped to 63.44% when the virus was identified without specifying the cell line. To the best of our knowledge, this is the first research article published in English to utilize AI for distinguishing animal virus infections in cell culture. AIRVIC’s hierarchical structure highlights its adaptability to virological diagnostics, providing unbiased infectivity scoring and facilitating viral isolation and antiviral efficacy testing. Additionally, AIRVIC is accessible as a web-based platform, allowing global researchers to leverage its capabilities in viral diagnostics and beyond.

Xue Du, Duoxi Li, Luya Jia et al.

Chicken semen cryopreservation is crucial for utilizing high-quality cockerel genetics, but semen is highly sensitive to cryoinjury, leading to poor preservation outcomes. This study aimed to establish a theoretical foundation for selecting cockerels for semen cryopreservation through serum testing and to improve semen quality via DNA methylation editing. Semen and serum samples were collected from 102 Xiaoshan cockerels, with semen cryopreserved and thawed following standardized protocols. Post-thaw semen quality and serum testosterone (T) levels were assessed. Eight cockerels were selected based on motile sperm quality, and whole-genome bisulfite sequencing (WGBS) was used to analyze sperm DNA methylation. The results showed a significant positive correlation between serum T levels and sperm motility. There were notable differences in sperm motility and serum T levels between high-quality and low-quality semen groups but no differences in estradiol (E₂), superoxide dismutase (SOD), or glutathione peroxidase (GSH-Px) levels. A total of 217 differentially methylated regions (DMRs) and 116 differentially methylated genes (DMGs) were identified. Key genes such as <i>PRKACB</i> (protein kinase, cAMP-dependent, catalytic, beta) and <i>ACSL1</i> (long-chain-fatty-acid--CoA ligase 1) were associated with sperm motility. These findings provide important insights for improving semen cryopreservation and contribute to breeding practices and the development of cryoprotectants.

Dickson Stuart Tayebwa, Dickson Stuart Tayebwa, Colin Ssekandi et al.

IntroductionUganda ranks among the top ten countries globally for reported dog bites, with an average of 14,865 cases annually, and records an estimated 486 human rabies deaths per year—partly due to the overwhelming number of free-roaming dogs (FRDs). Given that FRDs stem from societal mismanagement, sustainable control strategies must be rooted in meaningful community involvement. However, such community-based data remains largely unavailable in Uganda, hindering efforts to manage the growing FRD population.MethodsWe conducted a structured survey of 3,307 participants split between selected urban and rural areas in Uganda in 2023 to assess public attitudes and perceptions toward FRDs. Respondents were also asked to suggest appropriate control measures. We used chi-square tests to analyze sociodemographic differences and logistic regression to identify factors associated with attitudes toward FRDs.ResultsThe results showed that 81.9% of urban and 64.9% of rural respondents encountered FRDs daily. Attitudes toward FRDs were predominantly negative: 81.8% of respondents considered them a public health threat, and 57.1% viewed them as a nuisance. However, 76.3% also expressed empathy toward roaming dogs. Multivariable logistic regression analysis revealed that positive attitudes toward FRDs were associated with having a religious background, higher income, lower education levels, and residence in rural areas. Community-suggested control strategies included sterilization, public education on responsible dog ownership, and, controversially, poisoning.DiscussionThe findings highlight strong community awareness of the FRD issue, particularly as two of the top proposed measures—sterilization and public education—align with the World Organisation for Animal Health’s (WOAH) recommended strategies for managing roaming dog populations. Authorities can leverage the prevailing negative perceptions to design and implement humane, community-supported control strategies. Simultaneously, those who express empathy toward FRDs can be encouraged to translate their concern into proactive measures that reduce roaming behaviour.

I. M. Derkach, S. S. Derkach, Y. V. Zhuk et al.

One of the most relevant zoocides are rodenticides used to control harmful rodents. Carrying out effective deratization measures on the territory of Ukraine is especially important nowadays during the Russian-Ukrainian war. According to the reports of Ukrainian servicemen and civilians in the de-occupied territories, the population of rodents is extremely large, and not all modern rodenticide drugs lead to the death of harmful animals of these species. However, the toxicological characteristics of rodenticides take into account the fact that they can poison non-target animals. The purpose of our study was to analyze the pharmaco-toxicological characteristics of rodenticides and the main trends in the pharmaceutical market of drugs of this group registered in Ukraine as of January 1, 2024. It has been established that bromadiolone (77 %) and brodifacoum (23 %) are the main active substances in modern deratization agents. They belong to second-generation anticoagulants with a chronic mechanism of action. All registered rodenticides are produced in Ukraine and are available in various dosage forms. The low effectiveness of rodenticides can be due to falsification of starting substances for the synthesis of rodenticides, inconsistency of the required content of active substances, development of resistance of rodents to poisonous substances, etc. It is expected that the results highlighted in the article will indicate further directions in the development of new rodenticides and/or increase the effectiveness of the generally accepted scheme of deratization. In Ukraine, in the conditions of the Russian-Ukrainian war and in the post-war period, these questions are among the most significant, which scientists, pharmacologists and toxicologists, pharmacists and industry manufacturers should work on.

Yelis Tria Monica, Shanti Listyawati, Elisa Herawati

The use of paracetamol in excessive doses can increase the hepatotoxicity effect, which causes acute hepatocellular injury. Dewa leaves (Gynura pseudochina (L.) DC) contain flavonoids, saponin, and tannin. This study aimed to determine the effect of ethanol extract from dewa leaves in reducing the impact of paracetamol induction on SGPT and SGOT levels and liver histology of white rats (Rattus norvegicus). The test was grouped into five treatment groups, namely: normal control; negative control with paracetamol induction 315mg/KgBW; Dewa leaf extract was administered with three variations in dosage, namely 3.15mg/KgBW, 3.50mg/KgBW and 3.85mg/KgBW and each treatment group consisted of 5 rats as replications. Dewa leaf extract was administered to the experimental animal, followed by the administration of paracetamol 8 hours after the extract was given orally; both treatments were given for 14 days. Measurement of SGPT and SGOT levels uses the photometric method. The hepatoprotective effect was further verified by histopathology of the liver. Liver histology was made using the paraffin method. SGPT levels, SGOT levels, and Hepatocyte Damage Scores were analyzed using Analysis of Variance (ANOVA); if the results are significant, continue with the Least Significance Difference (LSD) test at a significance level of 5%. The results of this study show that the administration of dewa leaf extract was not able to significantly reduce SGPT and SGOT levels in rats. The administration of dewa leaf extract at doses of 3.50mg/KgBW and 3.85mg/KgBW significantly prevented histological damage to rat livers.