BackgroundOxidation-reduction and acid–base reactions are essential for the maintenance of all living organisms. However, redox potential (Eh) has received little attention in agronomy, unlike pH, which is regarded as a master variable. Agronomists are probably depriving themselves of a key factor in crop and soil science which could be a useful integrative tool.ScopeThis paper reviews the existing literature on Eh in various disciplines connected to agronomy, whether associated or not with pH, and then integrates this knowledge within a composite framework.ConclusionsThis transdisciplinary review offers evidence that Eh and pH are respectively and jointly major drivers of soil/plant/microorganism systems. Information on the roles of Eh and pH in plant and microorganism physiology and in soil genesis converges to form an operational framework for further studies of soil/plant/microorganism functioning. This framework is based on the hypothesis that plants physiologically function within a specific internal Eh-pH range and that, along with microorganisms, they alter Eh and pH in the rhizosphere to ensure homeostasis at the cell level. This new perspective could help in bridging several disciplines related to agronomy, and across micro and macro-scales. It should help to improve cropping systems design and management, in conventional, organic, and conservation agriculture.
We report here a mitochondria-targetable pH-sensitive probe that allows for a quantitative measurement of mitochondrial pH changes, as well as the real-time monitoring of pH-related physiological effects in live cells. This system consists of a piperazine-linked naphthalimide as a fluorescence off–on signaling unit, a cationic triphenylphosphonium group for mitochondrial targeting, and a reactive benzyl chloride subunit for mitochondrial fixation. It operates well in a mitochondrial environment within whole cells and displays a desirable off–on fluorescence response to mitochondrial acidification. Moreover, this probe allows for the monitoring of impaired mitochondria undergoing mitophagic elimination as the result of nutrient starvation. It thus allows for the monitoring of the organelle-specific dynamics associated with the conversion between physiological and pathological states.
Takaaki Takenaka, Takayuki Kubo, Imran Mahboob
et al.
We report on the internal quality factor of a three-dimensional niobium quarter-wave coaxial cavity, with mid-temperature annealing, exhibiting $Q_{\rm int} \gtrsim 3\times10^9$ at the single-photon level below 20\,mK, which corresponds to an internal photon lifetime of $τ_{\rm int}\sim90\,\mathrm{ms}$. Moreover, $Q_{\rm int}$ of the mid-temperature annealed cavities remains almost unchanged even after several cooldown cycles and air exposure. These results suggest that stable low-loss niobium oxides might be formed by mid-temperature annealing on the surface of three-dimensional niobium cavity. This surface treatment could be applicable to the fabrication of 2D superconducting circuits and help improve the lifetime of Nb-based superconducting qubits.
We propose and detail a multi-step analytical procedure, based on an improved fully relativistic plane model for Laser Wake Field Acceleration, to tailor the initial density of a cold diluted plasma to the laser pulse profile, so as to control and optimize the partial wave-breaking of the plasma wave and maximize the acceleration of small bunches of electrons self-injected by the first wave-breaking at the density down-ramp, at least in the first stages of their acceleration phase. We find an excellent agreement with the results of Particle In Cell simulations obtained with the same input data.
The dynamics of relativistic electrons interacting with a laser pulse in a plasma wave has been investigated theoretically and numerically based on the classical Landau-Lifshitz equation. There exists a convergent trajectory of electrons when the energy gain of electrons via direct laser acceleration can compensate the energy loss via radiation. An electron beam initially around the convergent trajectory evolves into the trajectory, making its occupied phase space volume decrease exponentially while mean energy remain the same. This mechanism can be used for cooling relativistic electron beams especially those produced in plasma-based acceleration.
An efficient approach that considers a high-intensity twisted laser of moderate energy (few J) is proposed to generate collimated proton bunches with multi-10-MeV energies from a double-layer hydrogen target. Three-dimensional particle-in-cell simulations demonstrate the formation of a highly collimated and energetic ($\sim 40$ MeV) proton bunch, whose divergence is $\sim 6.5$ times smaller compared to the proton bunch driven by a Gaussian laser containing the same energy. Supported by theoretical modeling of relativistic self-focusing in near-critical plasma, we establish a regime that allows for consistent acceleration of high-energetic proton bunches with low divergence under experimentally feasible conditions for twisted drivers.
Breakdown of dielectric RF windows is an important issue for particle accelerators and high-power RF sources. One of the common reasons for RF window failure is the multipactor on a dielectric surface. The multipactor may be responsible for excessive heating of the dielectric and discharge of charges that accumulated in the ceramic due to secondary emission. In this study, comprehensive self-consistent PIC simulations with space charge effect were performed. This was to better understand the dynamic of one-side multipactor development and floating potential on the dielectric induced by the emission. The important correlations between the multipactor parameters at saturation, the secondary emission properties of dielectric, and the applied RF field parameters have been found, which led to the conclusion that the dynamics of one-side multipactor on dielectric is a resonant phenomenon.
Protein phosphorylation is an important post-translational modification (PTM) involved in embryonic development, adult homeostasis, and disease. Over the past decade, several advances have been made in liquid chromatography-tandem mass spectrometry (LC-MS/MS)-based technologies to identify thousands of phosphorylation sites. However, in-depth phosphoproteomics often require off-line enrichment and fractionation techniques. In this study, we provide a detailed analysis of the physicochemical characteristics of phosphopeptides, which have been fractionated by off-line high-pH chromatography (HpH) before subsequent titanium dioxide (TiO2) enrichment and LC-MS/MS analysis. Our results demonstrate that HpH is superior to standard strong-cation exchange (SCX) fractionation in the total number of phosphopeptides detected when analyzing the same number of fractions by identical LC-MS/MS gradients. From 14 HpH fractions, we routinely identified over 30,000 unique phosphopeptide variants, which is more than twice the number of that obtained from SCX fractionation. HpH chromatography displayed an exceptional ability to fractionate singly phosphorylated peptides, with minor benefits for doubly phosphorylated peptides over that with SCX. Further optimizations in the pooling and concatenation strategy increased the total number of multiphosphorylated peptides detected after HpH fractionation. In conclusion, we provide a basic framework and resource for performing in-depth phosphoproteome studies utilizing off-line basic reversed-phased fractionation. Raw data is available at ProteomeXchange (PXD001404).