Semantic Scholar Open Access 2014 405 sitasi

Mitochondria-Immobilized pH-Sensitive Off–On Fluorescent Probe

M. H. Lee Nayoung Park C. Yi J. H. Han Ji Hye Hong +5 lainnya

Lihat Sumber DOI

Abstrak

We report here a mitochondria-targetable pH-sensitive probe that allows for a quantitative measurement of mitochondrial pH changes, as well as the real-time monitoring of pH-related physiological effects in live cells. This system consists of a piperazine-linked naphthalimide as a fluorescence off–on signaling unit, a cationic triphenylphosphonium group for mitochondrial targeting, and a reactive benzyl chloride subunit for mitochondrial fixation. It operates well in a mitochondrial environment within whole cells and displays a desirable off–on fluorescence response to mitochondrial acidification. Moreover, this probe allows for the monitoring of impaired mitochondria undergoing mitophagic elimination as the result of nutrient starvation. It thus allows for the monitoring of the organelle-specific dynamics associated with the conversion between physiological and pathological states.

Topik & Kata Kunci

Chemistry Medicine

Penulis (10)

M. H. Lee

Nayoung Park

C. Yi

J. H. Han

Ji Hye Hong

Kwang Pyo Kim

D. Kang

J. Sessler

Chulhun Kang

Jong Seung Kim

Format Sitasi

APA MLA BibTeX

Lee, M.H., Park, N., Yi, C., Han, J.H., Hong, J.H., Kim, K.P. et al. (2014). Mitochondria-Immobilized pH-Sensitive Off–On Fluorescent Probe. https://doi.org/10.1021/ja506301n

Akses Cepat

Lihat di Sumber doi.org/10.1021/ja506301n

Informasi Jurnal

Tahun Terbit: 2014
Bahasa: en
Total Sitasi: 405×
Sumber Database: Semantic Scholar
DOI: 10.1021/ja506301n
Akses: Open Access ✓