Using Local Estrogen with Implication of Modified Guillotine Technique to Improve Regenerative Process of pPartially Resected Liver in Rabbits

This research planned to determine the effect of local estrogen on the regeneration of partially hepatectomized rabbits. Twelve male rabbits were included in the present study, hereby allocated randomly into two equal groups. In the control group, partial hepatectomy was performed with the modified guillotine technique. In the treatment group, the steps are similar to the control (included partial hepatectomy) with the application of estrogen (0.2 mg). Macroscopic, laparoscopic, histopathological and immunohistochemical changes were recorded at 1, 2, and 4 weeks. Clinically, all rabbits survived a long period of experiment. Grossly, in the second group, 7 days post-surgery, there were no signs of inflammation and no intraoperative fluid. The results of laparoscopy 30 days in the control group indicated a change in liver color with severe adhesion between the liver and viscera; in the treatment group, the results indicated the presence of mild adhesion between the liver and viscera and normal liver structure and color. Histopathological results at 7 days in the control group showed cell swelling, congestion, and inflammatory cells, whereas the results at 30 days exhibited mild congestion of sinusoid, binucleate hepatocyte and fibrosis. In the treatment group, the results at 7 days indicated the presence of normal hepatocytes, fibrosis, and mild congestion in the sinusoid. At 30 days, results indicated the presence of normal hepatocytes, sinusoid and central veins. Immunoreactivity of tumor necrosis factor alpha (TNF-α) in the control group at 14 days indicated high expression, indicated as brown granules in hepatocytes, whereas in the treatment group at 14 days the expression was very mild as brown granules in hepatocytes. Based on laparoscopic, histopathological and immunohistochemistry changes, the application of estrogen on the hepatectomized site is beneficial and feasible to improve the regenerative process of the liver in rabbits.