Hasil untuk "hep-lat"

M. Ajello, A. Albert, W. Atwood et al.

The Fermi Large Area Telescope (LAT) has provided the most detailed view to date of the emission toward the Galactic center (GC) in high-energy γ-rays. This paper describes the analysis of data taken during the first 62 months of the mission in the energy range 1–100 GeV from a 15° × 15° region about the direction of the GC. Specialized interstellar emission models (IEMs) are constructed to enable the separation of the γ-ray emissions produced by cosmic ray particles interacting with the interstellar gas and radiation fields in the Milky Way into that from the inner ∼1 kpc surrounding the GC, and that from the rest of the Galaxy. A catalog of point sources for the 15° × 15° region is self-consistently constructed using these IEMs: the First Fermi-LAT Inner Galaxy Point Source Catalog (1FIG). The spatial locations, fluxes, and spectral properties of the 1FIG sources are presented, and compared with γ-ray point sources over the same region taken from existing catalogs. After subtracting the interstellar emission and point-source contributions a residual is found. If templates that peak toward the GC are used to model the positive residual the agreement with the data improves, but none of the additional templates tried account for all of its spatial structure. The spectrum of the positive residual modeled with these templates has a strong dependence on the choice of IEM.

E. Chan, J. Damoiseaux, O. G. Carballo et al.

During the 12th International Workshop on Autoantibodies and Autoimmunity held in Sao Paulo, Brazil, on August 28, 2014, a full day session was devoted to establishing a consensus on the nomenclature of staining patterns observed in the antinuclear antibody (ANA) indirect immunofluorescence test on HEp-2 cells. The current report summarizes the collective agreements with input from the host Brazilian and international communities that represented research, clinical, and diagnostic service laboratories. Patterns are categorized in three major groups (nuclear, cytoplasmic, and mitotic patterns) and each pattern has been defined and described in detail. The consensus nomenclature and representative patterns are made available online at the international consensus on antinuclear antibody pattern (ICAP) website (www.ANApatterns.org). To facilitate continuous improvement and input, specific comments on ICAP are encouraged and these will be discussed in subsequent ICAP meetings. The ultimate goal with the establishment of the ICAP is to promote harmonization and understanding of autoantibody test nomenclature, as well as interpretation guidelines for ANA testing, thereby optimizing usage in patient care.

M. Ackermann, R. Anantua, K. Asano et al.

On 2015 June 16, Fermi-LAT observed a giant outburst from the flat spectrum radio quasar 3C 279 with a peak >100 MeV flux of ∼3.6 × 10−5 photons cm−2 s−1, averaged over orbital period intervals. It is historically the highest γ-ray flux observed from the source, including past EGRET observations, with the γ-ray isotropic luminosity reaching ∼1049 erg s−1. During the outburst, the Fermi spacecraft, which has an orbital period of 95.4 minutes, was operated in a special pointing mode to optimize the exposure for 3C 279. For the first time, significant flux variability at sub-orbital timescales was found in blazar observations by Fermi-LAT. The source flux variability was resolved down to 2-minute binned timescales, with flux doubling times of less than 5 minutes. The observed minute-scale variability suggests a very compact emission region at hundreds of Schwarzschild radii from the central engine in conical jet models. A minimum bulk jet Lorentz factor (Γ) of 35 is necessary to avoid both internal γ-ray absorption and super-Eddington jet power. In the standard external radiation Comptonization scenario, Γ should be at least 50 to avoid overproducing the synchrotron self-Compton component. However, this predicts extremely low magnetization (∼5 × 10−4). Equipartition requires Γ as high as 120, unless the emitting region is a small fraction of the dissipation region. Alternatively, we consider γ rays originating as synchrotron radiation of γe ∼ 1.6 × 106 electrons, in a magnetic field B ∼ 1.3 kG, accelerated by strong electric fields E ∼ B in the process of magnetoluminescence. At such short distance scales, one cannot immediately exclude the production of γ-rays in hadronic processes.

William Y. C. Huang, Q. Yan, Wan-Chen Lin et al.

Jean-Marie Carpier, A. Zucchetti, Laurence Bataille et al.

The adapter molecule linker for activation of T cells (LAT) orchestrates the formation of signalosomes upon T cell receptor (TCR) stimulation. LAT is present in different intracellular pools and is dynamically recruited to the immune synapse upon stimulation. However, the intracellular traffic of LAT and its function in T lymphocyte activation are ill defined. We show herein that LAT, once internalized, transits through the Golgi–trans-Golgi network (TGN), where it is repolarized to the immune synapse. This retrograde transport of LAT depends on the small GTPase Rab6 and the target soluble N-ethylmaleimide-sensitive factor attachment protein receptor (t-SNARE) Syntaxin-16, two regulators of the endosome-to-Golgi/TGN retrograde transport. We also show in vitro in Syntaxin-16– or Rab6-silenced human cells and in vivo in CD4+ T lymphocytes of the Rab6 knockout mouse that this retrograde traffic controls TCR stimulation. These results establish that the retrograde traffic of LAT from the plasma membrane to the Golgi-TGN controls the polarized delivery of LAT at the immune synapse and T lymphocyte activation.

L. Balagopalan, Jason Yi, T. Nguyen et al.

The relative importance of plasma membrane-localized LAT versus vesicular LAT for microcluster formation and T-cell receptor (TCR) activation is unclear. Here, we show the sequence of events in LAT microcluster formation and vesicle delivery, using lattice light sheet microscopy to image a T cell from the earliest point of activation. A kinetic lag occurs between LAT microcluster formation and vesicular pool recruitment to the synapse. Correlative 3D light and electron microscopy show an absence of vesicles at microclusters at early times, but an abundance of vesicles as activation proceeds. Using TIRF-SIM to look at the activated T-cell surface with high resolution, we capture directed vesicle movement between microclusters on microtubules. We propose a model in which cell surface LAT is recruited rapidly and phosphorylated at sites of T-cell activation, while the vesicular pool is subsequently recruited and dynamically interacts with microclusters. Controversy exists over the function of plasma membrane versus intracellular vesicular LAT in T-cell receptor signaling. Here the authors use high resolution imaging of the temporal dynamics of LAT involvement to show that both sources of LAT are required, but at distinct stages.

H. Collaboration, H. Abdalla, A. Abramowski et al.

Centaurus A (Cen A) is the nearest radio galaxy discovered as a very-high-energy (VHE; 100 GeV–100 TeV) γ-ray source by the High Energy Stereoscopic System (H.E.S.S.). It is a faint VHE γ-ray emitter, though its VHE flux exceeds both the extrapolation from early Fermi-LAT observations as well as expectations from a (misaligned) single-zone synchrotron-self Compton (SSC) description. The latter satisfactorily reproduces the emission from Cen A at lower energies up to a few GeV. New observations with H.E.S.S., comparable in exposure time to those previously reported, were performed and eight years of Fermi-LAT data were accumulated to clarify the spectral characteristics of the γ-ray emission from the core of Cen A. The results allow us for the first time to achieve the goal of constructing a representative, contemporaneous γ-ray core spectrum of Cen A over almost five orders of magnitude in energy. Advanced analysis methods, including the template fitting method, allow detection in the VHE range of the core with a statistical significance of 12σ on the basis of 213 hours of total exposure time. The spectrum in the energy range of 250 GeV–6 TeV is compatible with a power-law function with a photon index Γ = 2.52 ± 0.13stat ± 0.20sys. An updated Fermi-LAT analysis provides evidence for spectral hardening by ΔΓ ≃ 0.4 ± 0.1 at γ-ray energies above 2.8+1.0−0.6 GeV at a level of 4.0σ. The fact that the spectrum hardens at GeV energies and extends into the VHE regime disfavour a single-zone SSC interpretation for the overall spectral energy distribution (SED) of the core and is suggestive of a new γ-ray emitting component connecting the high-energy emission above the break energy to the one observed at VHE energies. The absence of significant variability at both GeV and TeV energies does not yet allow disentanglement of the physical nature of this component, though a jet-related origin is possible and a simple two-zone SED model fit is provided to this end.

M. Ajello, A. Allafort, M. Axelsson et al.

We present the Fermi Large Area Telescope (LAT) observations of the binary neutron star merger event GW170817 and the associated short gamma-ray burst (SGRB) GRB 170817A detected by the Fermi Gamma-ray Burst Monitor. The LAT was entering the South Atlantic Anomaly at the time of the LIGO/Virgo trigger (tGW) and therefore cannot place constraints on the existence of high-energy (E > 100 MeV) emission associated with the moment of binary coalescence. We focus instead on constraining high-energy emission on longer timescales. No candidate electromagnetic counterpart was detected by the LAT on timescales of minutes, hours, or days after the LIGO/Virgo detection. The resulting flux upper bound (at 95% C.L.) from the LAT is 4.5 × 10−10 erg cm−2 s−1 in the 0.1–1 GeV range covering a period from tGW + 1153 s to tGW + 2027 s. At the distance of GRB 170817A, this flux upper bound corresponds to a luminosity upper bound of 9.7 × 1043 erg s−1, which is five orders of magnitude less luminous than the only other LAT SGRB with known redshift, GRB 090510. We also discuss the prospects for LAT detection of electromagnetic counterparts to future gravitational-wave events from Advanced LIGO/Virgo in the context of GW170817/GRB 170817A.

Yuexiang Li, L. Shen, Shiqi Yu

Siyamalan Manivannan, Wenqi Li, Shazia Akbar et al.

Immunofluorescence antinuclear antibody tests are important for diagnosis and management of autoimmune conditions; a key step that would benefit from reliable automation is the recognition of subcellular patterns suggestive of different diseases. We present a system to recognize such patterns, at cellular and specimen levels, in images of HEp-2 cells. Ensembles of SVMs were trained to classify cells into six classes based on sparse encoding of texture features with cell pyramids, capturing spatial, multi-scale structure. A similar approach was used to classify specimens into seven classes. Software implementations were submitted to an international contest hosted by ICPR 2014 (Performance Evaluation of Indirect Immunofluorescence Image Analysis Systems). Mean class accuracies obtained on heldout test data sets were 87.1% and 88.5% for cell and specimen classification respectively. These were the highest achieved in the competition, suggesting that our methods are state-of-the-art. We provide detailed descriptions and extensive experiments with various features and encoding methods. HighlightsWe propose systems for classifying immunofluorescence images of HEp-2 cells.Images are classified at both the cell level and the specimen level.Ensemble SVM classification based on sparse coding of texture features was effective.Cell pyramids and artificial dataset augmentation increased mean class accuracy.The proposed systems came first in the I3A contest associated with ICPR 2014.

C. Bacchelli, F. A. Moretti, M. Carmo et al.

Background: Signaling through the T‐cell receptor (TCR) is critical for T‐cell development and function. Linker for activation of T cells (LAT) is a transmembrane adaptor signaling molecule that is part of the TCR complex and essential for T‐cell development, as demonstrated by LAT‐deficient mice, which show a complete lack of peripheral T cells. Objective: We describe a pedigree affected by a severe combined immunodeficiency phenotype with absent T cells and normal B‐cell and natural killer cell numbers. A novel homozygous frameshift mutation in the gene encoding for LAT was identified in this kindred. Methods: Genetic, molecular, and functional analyses were used to identify and characterize the LAT defect. Clinical and immunologic analysis of patients was also performed and reported. Results: Homozygosity mapping was used to identify potential defective genes. Sanger sequencing of the LAT gene showed a mutation that resulted in a premature stop codon and protein truncation leading to complete loss of function and loss of expression of LAT in the affected family members. We also demonstrate loss of LAT expression and lack of TCR signaling restoration in LAT‐deficient cell lines reconstituted with a synthetic LAT gene bearing this severe combined immunodeficiency mutation. Conclusion: For the first time, the results of this study show that inherited LAT deficiency should be considered in patients with combined immunodeficiency with T‐cell abnormalities.

Ha Tran Hong Phan, Ashnil Kumar, Jinman Kim et al.

A. C. Lewin

Omri Matalon, S. Fried, Aviad Ben-Shmuel et al.

C. Cheung, P. Jean, P. Jean et al.

We report the Fermi Large Area Telescope (LAT) detections of high-energy (>100 MeV) γ-ray emission from two recent optically bright classical novae, V1369 Centauri 2013 and V5668 Sagittarii 2015. At early times, Fermi target-of-opportunity observations prompted by their optical discoveries provided enhanced LAT exposure that enabled the detections of γ-ray onsets beginning ∼2 days after their first optical peaks. Significant γ-ray emission was found extending to 39–55 days after their initial LAT detections, with systematically fainter and longer-duration emission compared to previous γ-ray-detected classical novae. These novae were distinguished by multiple bright optical peaks that encompassed the time spans of the observed γ-rays. The γ-ray light curves and spectra of the two novae are presented along with representative hadronic and leptonic models, and comparisons with other novae detected by the LAT are discussed.

Baerbel Keller, I. Zaidman, O. S. Yousefi et al.

Keller et al. describe for the first time human LAT deficiency, which causes severe immune dysregulation with autoimmunity, lymphoproliferation, and progressive immunodeficiency.

M. Ackermann, M. Ajello, A. Albert et al.

The Fermi Large Area Telescope (LAT) has an instantaneous field of view (FoV) covering ∼ 1 / 5 of the sky and it completes a survey of the entire sky in high-energy gamma-rays every 3 hr. It enables searches for transient phenomena over timescales from milliseconds to years. Among these phenomena could be electromagnetic counterparts to gravitational wave (GW) sources. In this paper, we present a detailed study of the LAT observations relevant to Laser Interferometer Gravitational-wave Observatory (LIGO) event GW150914, which is the first direct detection of gravitational waves and has been interpreted as being due to the coalescence of two stellar-mass black holes. The localization region for GW150914 was outside the LAT FoV at the time of the GW signal. However, as part of routine survey observations, the LAT observed the entire LIGO localization region within ∼70 minutes of the trigger and thus enabled a comprehensive search for a γ-ray counterpart to GW150914. The study of the LAT data presented here did not find any potential counterparts to GW150914, but it did provide limits on the presence of a transient counterpart above 100 MeV on timescales of hours to days over the entire GW150914 localization region.

H. Collaboration, H. Abdalla, A. Abramowski et al.

The supernova remnant (SNR) W49B originated from a core-collapse supernova that occurred between one and four thousand years ago, and subsequently evolved into a mixed-morphology remnant, which is interacting with molecular clouds (MC). γ-ray observations of SNR/MC associations are a powerful tool to constrain the origin of Galactic cosmic-rays, as they can probe the acceleration of hadrons through their interaction with the surrounding medium and subsequent emission of non-thermal photons. The detection of a γ-ray source coincident with W49B at very high energies (VHE; E > 100 GeV) with the H.E.S.S. Cherenkov telescopes is reported together with a study of the source with 5 years of Fermi-LAT high energy γ-ray (0.06 - 300 GeV) data. The smoothly-connected combined source spectrum, measured from 60 MeV to multi-TeV energies, shows two significant spectral breaks at 304±20 MeV and 8.4+2.2−2.5 GeV, the latter being constrained by the joint fit from the two instruments. The detected spectral features are similar to those observed in several other SNR/MC associations and are found to be indicative of γ-ray emission produced through neutral-pion decay.

Peter Hobson, B. Lovell, G. Percannella et al.

Xi Jia, L. Shen, Xiande Zhou et al.