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CrossRef Open Access 2021
Intervalos de referência para RDW-SD e RDW-CV em ovinos saudáveis

Polyana Mayume Silva

With the intention to stablish values of normality of sheep’s red blood cell distribution width (RDW), 72 blood samples from clinically healthy sheep were evaluated and sent to Clinical Pathology laboratory of FAMEZ- UFMS. The samples were processed in veterinary hematological analyzer, model pocH-100iV DIFF (Sysmex®), and the interval references stablished were 22,8 ± 1,8% for RDW CV and for 28,3 ± 2,8 fL RDW SD, these values presented can be used as references for healthy sheeps.

CrossRef 2023
Chromosome-Level Genome Assembly of Navel orange cv. Gannanzao ( <i>Citrus sinensis</i> Osbeck cv. Gannanzao)

Zhiwei Xiong, Hui Yin, Nian Wang et al.

Abstract Navel orange cv. Gannanzao is a variant of the navel orange cv. Newhall ( C. sinensis Osbeck cv. Newhall) that exhibits an earlier maturation, making it commercially valuable. However, the underlying mechanism underneath its early maturation remains unknown. To address this question, we conducted genome sequencing and de novo assembly of navel orange cv. Gannanzao. The assembled genome sequence is 334.57 Mb in length with a GC content of 31.48%. It comprises 318 contigs (N50 = 3.23 Mb) and 187 scaffolds (N50 = 31.86 Mb). The BUSCO test demonstrates 94.6% completeness. The annotation revealed 23,037 gene models, 164.95 Mb of repetitive sequences, and 2,554 ncRNA. Comparative analysis identified 323 fruit ripening-related genes in navel orange cv. Gannanzao genome, while navel orange cv. Newhall genome contained 345 such genes. These genes were organized into 320 orthologous gene families, with 30.3% of them exhibiting differences in gene copy numbers between the two genomes. Additionally, we identified 15 fruit ripening-related genes that have undergone adaptive evolution, suggesting their potential role in advancing fruit maturation in navel orange cv. Gannanzao. Whole genome sequencing and annotation of navel orange cv. Gannanzao provides a valuable resource to unravel the early maturation mechanism of citrus and enriches the genomic resources for citrus research.

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