This section includes a selection of book reviews, diary dates and other items of interest to nurse researchers. Contributions from readers are welcome as are letters discussing issues raised in this journal. Write to the Editor, NTResearch, Emap Healthcare, Porters South, Crinan Street, London N1 9XW. E-mail NTResearch@healthcare.emap.co.uk
This section includes a selection of book reviews, diary dates and other items of interest to nurse researchers. Contributions from readers are wekome as are letters discussing issues raised in this journal. Write to the Editor, NT Research, Macmillan Magazines, Porters South, Crinan Street, London N1 9XW. E-mail NTResearch@macmillan.com
A pandemia da Covid-19 paralisou as atividades presenciais de ensino-aprendizagem em todos os níveis, etapas e modalidades de ensino no Brasil em 2020. No momento em que este texto é escrito, findou-se o primeiro semestre do ano e passaram-se aproximadamente quatro meses desde a suspensão de aulas e de quaisquer outras atividades presenciais, da creche à pós-graduação. Diante da perspectiva de retorno parcial ou nulo às salas de aula ainda em 2020, impõe-se discutir alternativas para não ser um ano perdido nas redes públicas de ensino de todo o país. Trata-se de um debate complexo e multifacetário, pois, para além das soluções tecnológicas disponíveis, envolve letramento digital, formação continuada e diversas adaptações pedagógicas e gerenciais nada triviais que precisam ser pensadas e implementadas a toque de caixa.
Abstract INTRODUCTION: Resistance to conventional chemotherapy such as cisplatin, and radiotherapy in ovarian cancer may be caused by deregulated oncogenic miRNA-221/222 inhibiting induction of apoptosis. METHODS: Chemoradioresistant cells overexpressing EphA2 were obtained for developing an ovarian metastatic cancer model, which was treated with anti-EphA2 MAbs immunotargeted pegylated nanosomes with encapsulated paired guide-RNAs targeting 3' and 5' of the precursor microRNA 221/222. RESULTS: Post-treatment, the paired guide-RNAs caused deletions in the targeted pre-microRNA 221/222 genomic-sequences removing the stem-loop hairpins after blockade of RNase-III Drosha and DGCR8 (microprocessor-complex), subsequent transport by exportin-5/RanGTP and cleavage by Dicer inhibiting miRNA221/222 biogenesis, and miRNP circumventing their incorporation in RISC, and subsequent mRNA cleavage of coding genes regulating according to NGS the PTEN, GJA1, SEMA3B, TIMP3, PTPRM, CDKN1C, MGMT, CDKN1B, PUMA, connexin-43 (CX-43), c-Jun, MMP, NF-kB, PI3K, p27/Kip1, PTPμ, NIAP, ICAM-1, STAT1, STAT2, STAT3/Akt, CDK4, CDKN1C/p57, p53, and p57 inhibiting metastatic ovarian cancer cell proliferation, chemo/radioresistance, migration, angiogenesis, invasion, metastasis and maintenance of MET expressing ovarian metastatic cancer stem cells, while inducing cytotoxic immune responses (CTLs), and D2 irreversible stage of apoptosis or programmed cell death type-I leading to a bystander killing effect (BKE) due to phagocytosis of adjacent tumor cells according to transmission electron micrographs. CONCLUSION: Immunotargeted epigenomic nanosurgery may eradicate metastatic ovarian cancer after inhibition of oncogenic miRNA 221/222 expression, and downstream signaling pathways involving interactions with coding RNA genes leading to apoptosis or PCD type I and BKE after the circumvention of chemo/radioresistant mechanisms. Citation Format: John N. Giannios. ERADICATION OF CHEMORESISTANT METASTATIC OVARIAN CANCER WITH IMMUNOTARGETED EPIGENOMIC NANOSURGERY (IEN) [abstract]. In: Proceedings of the 12th Biennial Ovarian Cancer Research Symposium; Sep 13-15, 2018; Seattle, WA. Philadelphia (PA): AACR; Clin Cancer Res 2019;25(22 Suppl):Abstract nr NT-095.