Hasil untuk "q-bio.SC"

O. A. Makarov

Hong Shik Yun, Tamalee R. Kramp, Kamalakannan Palanichamy et al.

AbstractRadiotherapy is the standard treatment for glioblastoma (GBM), but the overall survival rate for radiotherapy treated GBM patients is poor. The use of adjuvant and concomitant temozolomide (TMZ) improves the outcome; however, the effectiveness of this treatment varies according to MGMT levels. Herein, we evaluated whether MGMT expression affected the radioresponse of human GBM, GBM stem-like cells (GSCs), and melanoma. Our results indicated a correlation between MGMT promoter methylation status and MGMT expression. MGMT-producing cell lines ACPK1, GBMJ1, A375, and MM415 displayed enhanced radiosensitivity when MGMT was silenced using siRNA or when inhibited by lomeguatrib, whereas the OSU61, NSC11, WM852, and WM266-4 cell lines, which do not normally produce MGMT, displayed reduced radiosensitivity when MGMT was overexpressed. Mechanistically lomeguatrib prolonged radiation-induced γH2AX retention in MGMT-producing cells without specific cell cycle changes, suggesting that lomeguatrib-induced radiosensitization in these cells is due to radiation-induced DNA double-stranded break (DSB) repair inhibition. The DNA-DSB repair inhibition resulted in cell death via mitotic catastrophe in MGMT-producing cells. Overall, our results demonstrate that MGMT expression regulates radioresponse in GBM, GSC, and melanoma, implying a role for MGMT as a target for radiosensitization.

Tikina R. Sethy, Arun K. Pradhan, Prafulla K. Sahoo

Gareth Jones, Sean Creaney

Universidade Federal da Fronteira Sul, UFFS, Chapecó, SC, Brasil, Jiennifer Souza de Oliveira, Ederson Nascimento et al.

This paper presents an analysis of the evolution of deaths caused by the COVID-19 pandemic in the municipality of Chapecó, State of Santa Catarina (Brazil). This is an ecological study, carried out through temporal analyzes using secondary data, which considers the number of deaths and the municipality’s health resources, from January 1st to July 31st, 2021. In this period, 562 deaths were registered of people with a confirmed diagnosis for COVID-19, of which 469 occurred between February and April. The profiles of such deaths were identified by sex and age group of each case. Overcrowding of beds was identified in the Intensive Care Units (ICUs), both in the Unified Health System (SUS) and in the private hospital network, especially in March, April, and May. Finally, it is highlighting the importance of vaccination to reduce mortality from the disease in the municipality.

Jani CP, Chovatia PK, Kathiriya SC et al.

B.R. Buck, J.K. Hollingsworth

Westinghouse Savannah River Co., Aiken, SC (United States), Sirrine Environmental Consultants, Greenville, SC (United States)