Advertising of veterinary medical services on social media
Aline Sousa, Shawana Gabriela de Jesus Chagas, Luany Gabrielly Santos Oliveira
et al.
This study presents a literature review based on the Veterinarian’s Code of Ethics, focusing on Chapter XIII – On Advertising and Scientific Works, with the aim of clarifying what is permitted and prohibited regarding veterinarians’ conduct on social media. The research highlights the growing presence of veterinary professionals in digital environments and identifies frequent ethical violations related to self-promotion and misinformation. By analyzing national regulations and comparing them with international ethical frameworks, the study emphasizes the need for clearer digital guidelines, stronger oversight by professional councils, and greater awareness among veterinarians about the responsible use of social media as an educational and ethical communication tool.
Applied research note: Predicting carcass portion weights for purebred turkey (Meleagris gallopavo) lines using a 2D imaging system
Shai Barbut, Emily M. Leishman, Ryley J. Vanderhout
et al.
Summary: Improving carcass portion yields (e.g., breast meat) is a major goal of modern turkey breeding and traditionally requires manual collection of portion weights. This can be a labor-intensive process considering the large amount of data needed to be useful for breeding companies. Recently, there has been increasing interest in using computer vision systems to assess parameters such as size, weight, volume, and grade of poultry meat. The present study developed mathematical equations to predict turkeys’ (4,000) meat yield using a non-invasive real-time 2D carcass imaging system. Although our breast meat models proved to be good, the thigh and drum models did not demonstrate a high correlation between observed and predicted weights probably due to the orientation of the image and any potential shifts made during image capture. These results represent a first step in developing prediction models for valuable turkey carcass portions using practical imaging systems. Further investigations need to take place to demonstrate this system can be more fruitful than simply predicting portion weight off live weight and help the industry to better collect phenotypes in a cost-effective manner.
Animal culture, Food processing and manufacture
A comparative metabolomics analysis of domestic yak (Bos grunniens) milk with human breast milk
Wenhao Li, Weike Zeng, Weike Zeng
et al.
Yaks are tough animals living in Tibet’s hypoxic stress environment. However, the metabolite composition of yak milk and its role in hypoxic stress tolerance remains largely unexplored. The similarities and differences between yak and human milk in hypoxic stress tolerance are also unclear. This study explored yak colostrum (YC) and yak mature milk (YMM) using GC–MS, and 354 metabolites were identified in yak milk. A comparative metabolomic analysis of yak and human milk metabolites showed that over 70% of metabolites were species-specific. Yak milk relies mainly on essential amino acids- arginine and essential branched-chain amino acids (BCAAs): L-isoleucine, L-leucine, and L-valine tolerate hypoxic stress. To slow hypoxic stress, human breast milk relies primarily on the neuroprotective effects of non-essential amino acids or derivates, such as citrulline, sarcosine, and creatine. In addition, metabolites related to hypoxic stress were significantly enriched in YC than in YMM. These results reveal the unique metabolite composition of yak and human milk and provide practical information for applying yak and human milk to hypoxic stress tolerance.
Lack of association between coat color abnormalities in Bali cattle (Bos javanicus) and the coding regions of the MC1R and KIT genes
Jakaria Jakaria, Kholijah Kholijah, Sri Darwati
et al.
Background and Aim: Coat color variations in cattle are known to be influenced by the melanocortin 1 receptor (MC1R) and receptor tyrosine kinase (KIT) genes. The presence of coat color abnormalities, such as white spots and albinism, in Bali cattle was the focus of this study. This study aimed to identify single nucleotide polymorphisms (SNPs) in the coding region of MC1R and exons 2 and 3 of KIT associated with coat color abnormalities in Bali cattle.
Materials and Methods: The study included the analysis of 48 Bali cattle, including 20 individuals with standard coat color, 15 with white spots, and 13 with albinism. Total DNA was extracted using a DNA Extraction Kit, and MC1R (coding region) and KIT (exons 2 and 3) gene amplifications were analyzed using forward and reverse primers with polymerase chain reaction product lengths of 1071, 234, and 448 bp, respectively. The determination of MC1R and KIT gene diversity was analyzed through direct sequencing. Melanocortin 1 receptor and KIT gene sequence data were analyzed using BioEdit and MEGA6 to identify SNPs associated with standard and abnormal coat color phenotypes (white-spotted and albino) in Bali cattle.
Results: No SNPs associated with coat color abnormalities were found in the coding region of MC1R and exons 2 and 3 of KIT genes in Bali cattle. However, the intron two regions of KIT contained the SNP g.70208534A>G, which showed a high degree of diversity. The AA genotype frequency was highest in albino Bali cattle, whereas the G allele frequency was highest and the A allele frequency was lowest in white-spotted Bali cattle.
Conclusion: The results indicated that standard, white-spotted, and albino coat colors in Bali cattle could not be distinguished by analyzing the MC1R and KIT genes.
Animal culture, Veterinary medicine
CRISPR-Cas12a test strip (CRISPR/CAST) package: In-situ detection of Brucella from infected livestock
Sheng Dang, Humujile Sui, Shuai Zhang
et al.
Abstract Background Brucellosis is a common zoonotic disease caused by Brucella, which causes enormous economic losses and public burden to epidemic areas. Early and precise diagnosis and timely culling of infected animals are crucial to prevent the infection and spread of Brucella. In recent years, RNA-guided CRISPR/Cas12a(Clustered Regularly Interspaced Short Palindromic Repeats and its associated protein 12a) nucleases have shown great promise in nucleic acid detection. This research aims to develop a CRISPR/CAST (CRISPR/Cas12a Test strip) package that can rapidly detect Brucella nucleic acid during on-site screening, especially on remote family pastures. The CRISPR/Cas12a system combined with recombinase polymerase amplification (RPA), and lateral flow read-out. Results We selected the conserved gene bp26, which commonly used in Brucella infection detection and compared on Genbank with other Brucella species. The genomes of Brucella abortus 2308, Brucella suis S2, Brucella melitansis 16 M, and Brucella suis 1330, et al. were aligned, and the sequences were found to be consistent. Therefore, the experiments were only performed on B. melitensis. With the CRISPR/CAST package, the assay of Brucella nucleic acid can be completed within 30 min under isothermal temperature conditions, with a sensitivity of 10 copies/μl. Additionally, no antigen cross-reaction was observed against Yersinia enterocolitica O:9, Escherichia coli O157, Salmonella enterica serovar Urbana O:30, and Francisella tularensis. The serum samples of 398 sheep and 100 cattle were tested by the CRISPR/CAST package, of which 31 sheep and 8 cattle were Brucella DNA positive. The detection rate was consistent with the qPCR results and higher than that of the Rose Bengal Test (RBT, 19 sheep and 5 cattle were serum positive). Conclusions The CRISPR/CAST package can accurately detect Brucella DNA in infected livestock within 30 min and exhibits several advantages, including simplicity, speed, high sensitivity, and strong specificity with no window period. In addition, no expensive equipment, standard laboratory, or professional operators are needed for the package. It is an effective tool for screening in the field and obtaining early, rapid diagnoses of Brucella infection. The package is an efficient tool for preventing and controlling epidemics.
Use of qualitative behavioural assessment to investigate affective states of housed dairy cows under different environmental conditions
Alison L. Russell, Laura V. Randall, Jasmeet Kaler
et al.
In addition to the reduction of suboptimal welfare, there is now a need to provide farmed animals with positive opportunities to provide confidence that they have experienced a life worth living. Diversification of the environment through environmental enrichment strategies is one suggested avenue for providing animals with opportunities for positive experiences. The provision of more stimulating environmental conditions has been widely implemented in other animal production industries, based on evidenced welfare benefits. However, the implementation of enrichment on dairy farms is limited. In addition to this, the relationship between enrichment and dairy cows' affective states is an under-researched area. One specific welfare benefit of enrichment strategies which has been observed in a number of species, is increased affective wellbeing. This study investigated whether the provision of different forms of environmental enrichment resources would impact the affective states of housed dairy cows. This was measured by Qualitative Behavioural Assessment, currently a promising positive welfare indicator. Two groups of cows experienced three treatment periods; (i) access to an indoor novel object, (ii) access to an outdoor concrete yard and (iii) simultaneous access to both resources. Principal component analysis was used to analyse qualitative behavioural assessment scores, which yielded two principal components. The first principal component was most positively associated with the terms “content/relaxed/positively occupied” and had the most negative associations with the terms ‘fearful/bored'. A second principal component was most positively associated with the terms “lively/inquisitive/playful” and was most negatively associated with the terms “apathetic/bored”. Treatment period had a significant effect on both principal components, with cows being assessed as more content, relaxed and positively occupied and less fearful and bored, during periods of access to additional environmental resources. Similarly, cows were scored as livelier, more inquisitive and less bored and apathetic, during treatment periods compared to standard housing conditions. Concurrent with research in other species, these results suggest that the provision of additional environmental resources facilitates positive experiences and therefore enhanced affective states for housed dairy cows.
Metabolic signature of Mycobacterium avium subsp. paratuberculosis infected and infectious dairy cattle by integrating nuclear magnetic resonance analysis and blood indices
Andrea Massaro, Alessandra Tata, Ivana Pallante
et al.
The early diagnosis of Mycobacterium avium subsp. paratuberculosis (MAP) is one of the current challenges of farmers and veterinarians. This work aimed to investigate the changes in metabolic levels associated with natural MAP infection in infected and infectious dairy cattle. The study included sera from 23 infectious/seropositive, 10 infected but non-infectious/seronegative, and 26 negative Holstein Fresian cattle. The samples were selected from a collection of samples gathered during a prospective study. The samples were analyzed by quantitative nuclear magnetic resonance (NMR) spectroscopy and routine blood chemistry. The blood indices and the 1H NMR data were concatenated by low-level data fusion, resulting in a unique global fingerprint. Afterwards, the merged dataset was statistically analyzed by the least absolute shrinkage and selection operator (LASSO), which is a shrinkage and selection method for supervised learning. Finally, pathways analysis was performed to get more insights on the possible dysregulated metabolic pathways. The LASSO model achieved, in a 10 time repeated 5-fold cross-validation, an overall accuracy of 91.5% with high values of sensitivity and specificity in classifying correctly the negative, infected, and infectious animals. The pathway analysis revealed MAP-infected cattle have increased tyrosine metabolism and enhanced phenylalanine, tyrosine and tryptophan biosynthesis. The enhanced synthesis and degradation of ketone bodies was observed both in infected and infectious cattle. In conclusion, fusing data from multiple sources has proved to be useful in exploring the altered metabolic pathways in MAP infection and potentially diagnosing negative animals within paratuberculosis-infected herds.
Complete bladder eversion in periparturient Nellore cow - case report
J.R.B. Silva, C.A.E. Baumam, A.P.A. Mendonça
et al.
ABSTRACT Partial or complete bladder eversion is a rare condition of poor prognosis in cows, commonly associated with intense tenesmus observed in the peripartum period. A 14-year-old obese Nellore cow at 280 days of gestation was referred with 24-hour bladder prolapse. The bladder was complete eversion through the vulvar vestibule showing a thick congested wall and small residual urine volume. After clinical examination, the cow received scopolamine butylbromide and intercoccygeal epidural anesthesia, and the externalized bladder segment was partially reduced, remaining only 10 cm externalized. The cow was maintained with an intravesical human gastric tube number 16 and constant monitoring. The eversion was fully reduced after 12 hours of local treatment, and as the cow presented subclinical ketosis, hypocalcemia and cystitis, antibiotic, glucose, calcium and propylene glycol therapy were performed. We opted for induction of parturition, and after 24 hours, a healthy 52kg calf was born and the placenta was delivered 16 hours after calving. The cow and calf were discharged on the sixth day of hospitalization, with no recurrences or secondary complications after treatment.
A postbiotic from Aspergillus oryzae attenuates the impact of heat stress in ectothermic and endothermic organisms
J. D. Kaufman, Y. Seidler, H. R. Bailey
et al.
Abstract Heat stress is detrimental to food-producing animals and animal productivity remains suboptimal despite the use of heat abatement strategies during summer. Global warming and the increase of frequency and intensity of heatwaves are likely to continue and, thus, exacerbate the problem of heat stress. Heat stress leads to the impairment of physiological and cellular functions of ectothermic and endothermic animals. Therefore, it is critical to conceive ways of protecting animals against the pathological effects of heat stress. In experiments with endothermic animals highly sensitive to heat (Bos taurus), we have previously reported that heat-induced systemic inflammation can be ameliorated in part by nutritional interventions. The experiments conducted in this report described molecular and physiological adaptations to heat stress using Drosophila melanogaster and dairy cow models. In this report, we expand previous work by first demonstrating that the addition of a postbiotic from Aspergillus oryzae (AO) into the culture medium of ectothermic animals (Drosophila melanogaster) improved survival to heat stress from 30 to 58%. This response was associated with downregulation of genes involved in the modulation of oxidative stress and immunity, most notably metallothionein B, C, and D. In line with these results, we subsequently showed that the supplementation with the AO postbiotic to lactating dairy cows experiencing heat stress decreased plasma concentrations of serum amyloid A and lipopolysaccharide-binding protein, and the expression of interleukin-6 in white blood cells. These alterations were paralleled by increased synthesis of energy-corrected milk and milk components, suggesting enhanced nutrient partitioning to lactogenesis and increased metabolic efficiency. In summary, this work provides evidence that a postbiotic from AO enhances thermal tolerance likely through a mechanism that entails reduced inflammation.
Evaluación preliminar de la aptitud simbiótica de cuatro materiales de alfalfa (Medicago sativa L.)
R. L. Cismondi, C. A. Sánchez
En la Facultad de Agronomía de la UNLPam se realizó una experiencia tendiente a evaluar la aptitud umbi6tica de los siguientes materiales de alfalfa: (1) Anguil INTA. (2) Intacic original, (3) Intacic seleccionada por su aptitud de fijación simbiótica y (4) MN Root (nitro 5887 Dames) seleccionada en la Universidad Minnesota por su aptitud de fijación simbiótica. Los materiales fueron cultivados en invernáculos, utilizando caños de PVC de 6.25 cm de diámetro y 50 cm de largo. En cada caño se cultivó una planta. A los 88 días de la siembra se realizó la extracción de las. plantas, determinándose: (a) altura de plantas, (b) número de hojas desplegadas, (e) nódulos en raíz principal, (d) nódulos en raíces secundarias, (e) peso seco sistema radical, (f) peso seco parte aérea, (g) biomasa total, (h) porcentaje de nitrógeno en el sistema radical, (i) porcentaje de nitr6geno en parte aérea. los materiales seleccionados (3) y (4) mostraron diferencias signi6cativas con respecto a los no seleccionados (1) y (2) en los siguientes parámetros: (a) altura de plantas, (d) nódulos en raíces secundarias, (e) peso seco sistema radical, (f) peso seco parte aérea, (g) biomasa total. Aunque no hubo diferencias significativas en el porcentaje de nitr6geno de los materiales evaluados, llevando los datos a cantidad de nitrógeno presente por planta, las diferencias fueron significativas, tanto en sistema radical como en parte aérea. La información lograda permite estimar que efectivamente, los materiales Intacic seleccionada y MN Root (nitro 5887 Bames) tienen mayor aptitud de fijación simbiótica. Intacic seleccionada se destaca sobre MN Root (nitro 5887 Bames) en n. mero de hojas desplegadas, peso seco parte aérea y cantidad de nódulos en raíces secundarias. Consideramos que Intacic seleccionada tiene posibilidades de ser introducida al cultivo, como un cultivar con buenas características agronómicas y una aptitud de fijación simbiótica que supera a los materiales bajo cultivo en Argentina, y posiblemente en algunos parámetros, también a los materiales extranjeros.
Director: Ing. Agr. O. Hernández. Profesor Titular de Forrajicultura y Manejo de Pasturas.
Agriculture (General), Animal culture
TRANSPLANTATION IN MINIATURE SWINE: I. Fixation of the Major Histocompatibility Complex
D. Sachs, G. Leight, J. Cone
et al.
461 sitasi
en
Biology, Medicine
Os pronomes cosmológicos e o perspectivismo ameríndio
E. Castro
This study discusses the meaning of Amerindian "perspectivism": the ideas in Amazonian cosmologies concerning the way in which humans, animals, and spirits see both themselves and other world beings. Such ideas suggest the possibility of a redefinition of the classical categories of "nature", "culture", and "supernature" based on the concept of perspective or point of view. The study argues in particular that the antinomy between two characterizations of indigenous thought - on the one hand "ethnocentrism", which would deny the attributes of humanity to humans from other groups, and on the other hand "animism", which would extend such qualities to beings from other species - can be resolved if one considers the difference between the spiritual and corporal aspects of beings.
Alkaloids: biochemistry, ecology, and medicinal applications.
M. Roberts, M. Wink
Quiet please, do not disturb: a hypothesis of embryo metabolism and viability.
H. Leese
369 sitasi
en
Biology, Medicine
Net-bottom Cage Inserts for Water Bird Casualties
Jackie Belle
<p>My Bright Idea is a net-bottomed cage insert, which is used to support pelagic avian casualties. The idea was designed and modified by the <a href="https://www.bird-rescue.org/about/overview.aspx" target="_blank">International Bird Rescue in California</a> (Bird Rescue).</p><br /> <img src="https://www.veterinaryevidence.org/rcvskmod/icons/oa-icon.jpg" alt="Open Access" /> <img src="https://www.veterinaryevidence.org/rcvskmod/icons/pr-icon.jpg" alt="Peer Reviewed" />
Determining the safety of microbial cultures for consumption by humans and animals.
M. Pariza, K. Gillies, Sarah F Kraak-Ripple
et al.
63 sitasi
en
Biology, Medicine
Designer self-assembling peptide nanofiber scaffolds for 3D tissue cell cultures.
Shuguang Zhang, F. Gelain, Xiaojun Zhao
359 sitasi
en
Computer Science, Medicine
Inhibitory effect of Bifidobacterium longum on colon, mammary, and liver carcinogenesis induced by 2-amino-3-methylimidazo[4,5-f]quinoline, a food mutagen.
Bandaru S. Reddy, A. Rivenson
353 sitasi
en
Biology, Medicine
Streptococcus agalactiae mastitis: a review.
Gregory P. Keefe
348 sitasi
en
Medicine, Biology
Incidence of Aeromonas spp. infection in fish and chicken meat and its related public health hazards: A review
Praveen Kumar Praveen , Chanchal Debnath , Shashank Shekhar
et al.
Aeromonas is recognized to cause a variety of diseases in man. In humans, they are associated with intestinal and extraintestinal infections. With the growing importance of Aeromonas as an emerging pathogen, it is important to combat this organism. It is indisputable that Aeromonas strains may produce many different putative virulence factors such as enterotoxins, hemolysins or cytotoxins, and antibiotic resistance against different antibiotics. The ability of these bacteria to grow competitively at 5°C may be indicative of their potential as a public health hazard. Comprehensive enteric disease surveillance strategies, prevention and education are essential for meeting the challenges in the years ahead. It is important for us to promote the value of enteric cultures when patients have a gastrointestinal illness or bloody diarrhea or when multiple cases of enteric disease occur after a common exposure. With the growing importance of Aeromonas as an emerging pathogen, it is important to combat this organism. It is indisputable that Aeromonas strains may produce many different putative virulence factors, such as enterotoxins, hemolysins or cytotoxins. It has been established that aerolysin is a virulence factor contributing to the pathogenesis of Aeromonas hydrophila infection. Fish and chicken play an important role in the transmission of this pathogen to humans. In the present study, the high prevalence of toxin-producing strains was found among the Aeromonas isolates. The ability of these bacteria to grow competitively at 5°C may be indicative of their potential as a public health hazard. The present review was constructed with a view to highlight the zoonotic importance of Aeromonas pathogen in fish and chicken meat.
Animal culture, Veterinary medicine