Hasil untuk "Homeopathy"

D. Riemann, C. Baglioni, C. Bassetti et al.

Delight Hwarari, Yuanlin Guan, Baseer Ahmad et al.

Cold stress limits plant geographical distribution and influences plant growth, development, and yields. Plants as sessile organisms have evolved complex biochemical and physiological mechanisms to adapt to cold stress. These mechanisms are regulated by a series of transcription factors and proteins for efficient cold stress acclimation. It has been established that the ICE-CBF-COR signaling pathway in plants regulates how plants acclimatize to cold stress. Cold stress is perceived by receptor proteins, triggering signal transduction, and Inducer of CBF Expression (ICE) genes are activated and regulated, consequently upregulating the transcription and expression of the C-repeat Binding Factor (CBF) genes. The CBF protein binds to the C-repeat/Dehydration Responsive Element (CRT/DRE), a homeopathic element of the Cold Regulated genes (COR gene) promoter, activating their transcription. Transcriptional regulations and post-translational modifications regulate and modify these entities at different response levels by altering their expression or activities in the signaling cascade. These activities then lead to efficient cold stress tolerance. This paper contains a concise summary of the ICE-CBF-COR pathway elucidating on the cross interconnections with other repressors, inhibitors, and activators to induce cold stress acclimation in plants.

Jason Gandhi, Andrew Chen, G. Dagur et al.

Anil Kumar, Anil Kumar K. V., Swaroopa Rani et al.

Abstract Background Urolithiasis is a painful condition and current treatment doesn’t assure the prevention of recurrence. This research aims to demonstrate the scientific reliability of Chloroform leaf extract of Flemingia Strobilifera R.Br. (CEFS) for antiurolithiatic activity using ethylene glycol-induced urolithiasis model. Results Ethylene glycol (EG) was used to induce hyperoxaluria in male Wistar rats. The rats were grouped into 7-groups containing six each. Group I and II served as negative and positive control, group III received standard treatment, whereas Group IV to VII served as testing groups. CEFS of 30 mg/kg body-weight and 60 mg/kg body-weight was used as a preventive and curative regimen. The urine biochemistry was analysed for the presence of calcium, magnesium, phosphate, and oxalate. The rats were sacrificed for histopathological examination and LDH detection. The 24-hours urine volume was increased in both EG-treated groups as well as CEFS-treated groups, indicating the diuretic activity of plant. CEFS dose-dependently inhibited urine excretion of phosphate, calcium, and oxalate compared to the positive-control group. The histopathological examination of CEFS-treated rats’ kidneys had reduced loss of renal structure and lessened deposition of calcium oxalate crystals. Conclusion CEFS exhibited significant antiurolithiatic activity by reducing supersaturation of urine and excretion of stone forming components.

Christophe Deraedt, D. Astruc

Abdulkerim Mustefa, Aschalew Nardos, Dejene Hailu et al.

Abstract Background Despite significant advances in synthetic medicine, medicinal plants are still widely utilized to treat peptic ulcer disease. This study synthesized evidence on commonly used medicinal plants to manage peptic ulcer disease in Ethiopia. Methods The evidence was synthesized using electronic databases such as PubMed/Medline, Web of Science, Science Direct, Hinari, and Google Scholar. This review considers all controlled in vivo and ex vivo anti-ulcer studies evaluating Ethiopian medicinal plants with regards to phytochemistry, efficacy, and safety. The search technique covered all published papers using descriptors like Ethiopia, medicinal plants, and anti-ulcer until January 30, 2022. The percentage of ulcer reduction was used to measure the success of the treatment. Results Anti-ulcer properties were investigated in 13 papers. Twelve in vivo and one ex vivo study were retrieved. Osyris quadripartita, Plantago lanceolata, Ensete ventricosum, Taverniera abyssinicia, Crotonmacrostachyus Persea Americana and Moringa stenopetala showed effect close to a standard control in difference ulcer induced model. Osyris quadripartite in the dose of 200 mg/kg, Ficus thonningii in dose of 200 and 400 mg/kg, Plantago lanceolata in dose of 400 mg/kg, Trigonella feonum-gracum in dose of 500 and 1000 mg/kg, Linum ussitatissimum in dose of 1000 and 1500 mg/kg, Urtica simensis 400 mg/kg have higher activity compared with standard control in difference different models. Phytochemistry studies revealed presence of numerous components, including flavonoids, phenols, tannins, and saponins, which may be responsible for the anti-ulcer activity. Conclusion This study revealed that there are some promising medicinal plant extracts that have been used in Ethiopia for centuries that could be used as anti-ulcer agents. The most effective anti-ulcer agents in animal models are Indigofera spicata Forssk, Thymus schimperi, and Urtica simensis. Identification of most active pharmacological agents and clinical evaluation of Ethiopian medicinal plants used to treat peptic ulcers are worthy of further investigation.

J. Solomon

S. Chen, Hsu NH-Mi, J. Kleijnen et al.

Amina Khatun, Mahmudur Rahman, Mst. Luthfun Nesa et al.

Abstract Background Cestrum diurnum L. (Solanaceae), locally known as buno-Hasnahena, is widely used in different traditional medicinal practices to treat pain, burn, swelling and related disorders. Adequate evidence is not available to support its medicinal properties for further use and drug development. Present study was designed to evaluate its traditional use in pain and inflammation with further characterisation of its chemical constituents through liquid chromatography-mass spectroscopic (LC-MS) analysis. Methods Antinociceptive and analgesic potential of methanol extract of the aerial parts of C. diurnum was carried out using carrageenan induced paw oedema and formalin induced paw licking test in mice at the oral doses of 150 and 300 mg/kg body weight. Inhibition of the inflammatory mediator nuclear factor kappa B (NF-κB) was evaluated by tumour necrosis factor α (TNF-α) induced NF-κB activation assay in macrophage cells at the concentration of 100 μg/ml. LC-MS analysis of the extract was performed to characterise the active component responsible for bioactivities. Results The extract significantly inhibited (p < 0.05) carrageenan induced paw oedema at both doses tested and the effect persisted throughout the entire experimental period of 3 h with the highest activity (50% inhibition) observed at 3rd h. Further, the extract significantly inhibited (p < 0.05) formalin induced paw licking both in the early and late phase at the aforementioned dose levels. The extract also downregulated the expression of NF-κB p65 protein in the TNF-α induced NF-κB activation assay. LC-MS analysis of the extract indicated the presence of some important secondary metabolites including nicotine, nornicotine, ursolic acid, vitamin D3 and its derivatives. Conclusions The results of this study supported the folkloric uses of the plant in pain and inflammations. The insights and observations suggest the action might involve downregulation of NF-κB p65 protein expression and/or inhibition of autacoids (histamine, serotonin, prostaglandin).

M. Wetzel, D. Eisenberg, T. Kaptchuk

Dhananjay Tandon, A. K. Gupta

Abstract Background Sphaeranthus indicus is an important medicinal plant, which is used to cure various illnesses. The present study is the first investigation of the antimicrobial, antioxidant and phytochemical analysis of Sphaeranthus indicus from Chhattisgarh, India. Methods The whole plant and plant parts were extracted with polar and non-polar solvents such as water, methanol, ethyl acetate and hexane to assess various bioactivities. The antimicrobial and antioxidant activities were performed by ager well diffusion method and ferrous reducing capacity, respectively; However free radical scavenging activity was analyzed using DMPD and DPPH scavenging assay. The DMPD and DPPH assay were performed in a time dependent manner. Qualitative and quantitative analysis were performed for the total phytochemicals present in the plant extracts. The total content of phenols, flavonoids and terpenoids was analyzed by colorimetric methods. Results Ethyl acetate and hexane extract of plant inflorescence and stem exhibited significant antibacterial activity against tested bacterial pathogens. The clinically isolated gram positive pathogenic bacteria responded better as compared to clinically isolated gram negative bacteria as well as pathogenic gram positive and negative bacteria acquired from Microbial Type Culture Collection, India. The leaf and inflorescence exhibited potent antioxidant activity. The polar fraction of leaf methanol extract exhibited the highest reducing power capacity. The aqueous extract of inflorescence exhibited highest inhibition against DMPD and DPPH radicals. The whole plant aqueous extract showed maximum inhibition while aqueous extract of inflorescence exhibited high inhibition among different plant parts. Qualitative phytochemical analysis revealed the presence of terpenoids, phenols, flavonoids, tannins and cardiac glycosides in plant parts. Total terpenoid content was found to be highest in polar fraction of stem methanol extract and ethyl acetate extract of inflorescence. However, total phenol was found to be highest in polar fraction of leaf methanol extract, similarly highest flavonoid content was observed in aqueous extract of leaf. Conclusion The results suggest that biological activities of plant parts depend on content of active phytochemicals. The inflorescence could be a potential source of antimicrobial and antioxidant compound. Further, investigation pertaining isolation and characterization of active ingredient may provide an insight regarding its phytochemical activity.

Zainab Aliyu Alhafiz, Mohammed Sani Abdulsalami, Mohammed Auwal Ibrahim et al.

Abstract Objective The present study investigated the anti-Trypanosoma brucei brucei activity of methanolic extract of Senna occidentalis roots, leaves, stem bark, and seed in vitro and bioactive components of the most active plant part. Materials and methods Trypanosoma brucei brucei was identified by PCR, cultured in Dulbecco’s Modified Eagle Medium (DMEM) and incubated at 37 °C and 5% CO2. Subsequently, the cultured T. brucei brucei were incubated with 500 μg/ml concentration of different parts of S. occidentalis plant and the most potent fraction was identified and subjected to Gas Chromatography-Mass Spectrometry (GC-MS). Results Amplicons of the rRNA gene of T. brucei brucei detected had a size of 1200 bp. The anti-trypanosomal activities indicated that the root extract of the plant was the most active at 500 μg/ml with inhibitory activity of 88.89% and fractions (2 and 5) the most active with IC50 values of 1.49 and 1.16 μg/ml respectively. Conclusion Results from this study insinuates that phenolic and simple aliphatic compounds might play key role in the anti-trypanosomal activity of S. occidentalis roots.

Leila Verônica da Costa Albuquerque, José Wellington de Oliveira Lima, Ana Beatriz Gois da Silva et al.

ABSTRACT Context Complementary and Alternative Medicine (CAM) are unconventional care practices that, according to the World Health Organization, should be implemented in the Health System, particularly in poor regions of the country. In Brazil, they have been adopted by Care Programs and introduced into undergraduate medical education. In this study we were interested in evaluating the teaching-learning process of Integrative Practices in Brazilian medical schools. Methodology A cross-sectional study was carried out at Brazilian medical schools with a self-administered questionnaire for teachers and a secondary data survey was obtained from medical school websites and government database institutions. For the presentation of the variables, frequency distribution and the Pearson coefficient (X2) -Chi-square tests were used. The proportions were compared using the Chi-square test or the Fisher’s Exact Test. When the expected value of a contingency table was equal to or greater than 5, the Chi-square test was used; in all other situations, Fisher’s Exact Test was used. The difference between proportions was estimated by the Odds Ratio, calculated through simple logistic regression (95% CI). Results 57 of the 272 medical schools in Brazil address CAM, with it being proportionally higher in the South and Mid-West regions. The medical schools are highly concentrated in state capitals, and the Northeast region presents a significant concentration of medical schools with CAM in the capitals. The number of schools with active and traditional methodologies in CAM is equivalent. Homeopathy, Acupuncture and Integrative Medicine predominate, with a minority using Indigenous Practices, Chronotherapy and Anthroposophic Medicine. The new educational guidelines have not affected the number of schools with CAM. Growth in CAM has been insignificant (p <0.05) in the last ten years. Conclusion There has been no growth in teaching Complementary and Alternative Medicine in undergraduate medical training in Brazil since the introduction of the new curricular guidelines, even in view of the needs of the health system.

Faiza Ashfaq, Masood Sadiq Butt, Ahmad Bilal et al.

Abstract Background The shift towards hypercaloric diets and sedentariness has raised lifestyle related disorders and escalated health care cost. In order to tackle this epidemiological transition, healthy, affordable food choices should be introduced in the routine menu. In this context, red cabbage is known for its rich phytochemistry, minerals, antioxidant vitamins and dietary fiber. Considering these evidences, red cabbage leaves and its extract were assessed against hypercholesterolemia and associated oxidative stress. Methods In bioefficacy assessment trial (12 weeks), there were two dietary regimens; normal and hypercholesterolemic (1% cholesterol) that were further split into three groups each. In both feeding trials, red cabbage leaves (20%) and its extract in dose equivalent to red cabbage leaves were assessed against control diets. At termination of trial, serum lipidemic parameters and oxidative stress biomarkers were assessed to test the efficacy of diets. Results In hypercholesterolemic rabbits, red cabbage leaves showed significant reduction in cholesterol, LDL-c and triacylglycerol levels i.e. 15.19, 18.09 and 9.42% than extract administered groups; 10.79, 12.24 and 5.72, respectively. Besides, red cabbage leaves also portrayed momentous enhancement of superoxide dismutase (SOD) and catalase (CAT) activity up to 13.29 & 17.63% by lowering lipid peroxidation by 27.86% in hypercholesterolemic diet fed groups, whereas red cabbage extract administered group depicted relatively lesser amelioration in lipid peroxidation i.e. 21.42%. Conclusions Red cabbage leaves possess higher ameliorative potential against altered lipidemic profile and lipid peroxidation as compared to its extract thus explains its ability to prevent exhaustion of endogenous antioxidant enzymes; SOD and CAT.

Peter Masoko PhD, Maano V. Masiphephethu MSc

The focus of this study was to evaluate the antioxidants and antimycobacterial activities of extracts of Schkuhria pinnata . Serial exhaustive extraction procedure was employed using solvents of varying polarity to obtain the desired extracts. Thin layer chromatography and standard chemical tests were used to analyze phytochemicals constituents. Free radical scavenging 2,2-diphenyl-1-picrylhydrazyl (DPPH) methods were used to detect the presence of antioxidant compounds. Antimycobacterial activity was evaluated using microdilution and bioautography assays. A variety of secondary metabolites such as flavonoids, tannins, and alkaloids were detected in the extract. Ethyl acetate and acetone extracts had high antioxidant activity on chromatograms eluted in ethyl acetate/methanol/water while methanol extract at various concentrations had the best scavenging activity. The minimum inhibitory concentration (MIC) values ranged from 0.02 to 2.50 mg/mL. Total phenol content was 55.33 ± 3.51 mg of gallic acid equivalent (GAE)/g and higher when compared with flavonoids (4.00 ± 0.35 mg of quercetin equivalent [QE]/mg) and tannin content (28.00 ± 1.73 mg of GAE/g). The most effective antimycobacterial activity against Mycobacterium smegmatis was observed with the lowest inhibitory concentrations of acetone (0.27 mg/mL), dichloromethane (0.32 mg/mL), and ethyl acetate (0.32 mg/mL) in that order. In massive extraction, hexane and dichloromethane had the greatest inhibitory bands on benzene/ethanol/ammonium hydroxide bioautograms. Antimmycobacterial activity gives promising potential leads of S pinnata extracts to be used in the development of antimycobacterial drugs. The presence of antioxidant and antimycobacterial compounds requires further isolation and purification.

Sweta Shanbhag, Rashmi Alva, K Shreedhara Avabratha

Acute myocarditis and dilated cardiomyopathy are known to be common manifestations of several viral infections, presenting with a wide spectrum of symptoms. The exact pathogenesis of influenza virus-induced myocarditis is still unclear. Management involves early detection using echocardiography and cardiac support to tide over acute phase. Following recovery, prognosis is known to be quite good; however, fulminant cases have poor prognosis. A 2-year-old female infant previously diagnosed to have H1N1 bronchopneumonia a year back, who was found to have compromised cardiac function suggestive of dilated cardiomyopathy on follow-up and was started on oral antifailure medications and kept on regular follow-up, presented to the emergency department with symptoms suggestive of severe form of dilated cardiomyopathy and succumbed to illness. The association between influenza viral infection and myocarditis is being linked with host immune response as well as direct cellular damage. Vaccination is being said to be the most cost-effective strategy to prevent this potentially fatal complication. Recent-onset cardiac symptomatology with a preceding history suggestive of influenza infection warrants a high index of suspicion for myocarditis and dilated cardiomyopathy. Early detection and prompt cardiac supportive care and management are paramount and help to reduce morbidity and mortality.

Sreemanti Das, Jayeeta Das, A. Samadder et al.

F. Borrelli, E. Ernst

Ferrán Catalá-López, Brian Hutton, Amparo Núñez-Beltrán et al.

<h4>Background</h4>Attention deficit hyperactivity disorder (ADHD) is one of the most commonly diagnosed psychiatric disorders in childhood. A wide variety of treatments have been used for the management of ADHD. We aimed to compare the efficacy and safety of pharmacological, psychological and complementary and alternative medicine interventions for the treatment of ADHD in children and adolescents.<h4>Methods and findings</h4>We performed a systematic review with network meta-analyses. Randomised controlled trials (≥ 3 weeks follow-up) were identified from published and unpublished sources through searches in PubMed and the Cochrane Library (up to April 7, 2016). Interventions of interest were pharmacological (stimulants, non-stimulants, antidepressants, antipsychotics, and other unlicensed drugs), psychological (behavioural, cognitive training and neurofeedback) and complementary and alternative medicine (dietary therapy, fatty acids, amino acids, minerals, herbal therapy, homeopathy, and physical activity). The primary outcomes were efficacy (treatment response) and acceptability (all-cause discontinuation). Secondary outcomes included discontinuation due to adverse events (tolerability), as well as serious adverse events and specific adverse events. Random-effects Bayesian network meta-analyses were conducted to obtain estimates as odds ratios (ORs) with 95% credibility intervals. We analysed interventions by class and individually. 190 randomised trials (52 different interventions grouped in 32 therapeutic classes) that enrolled 26114 participants with ADHD were included in complex networks. At the class level, behavioural therapy (alone or in combination with stimulants), stimulants, and non-stimulant seemed significantly more efficacious than placebo. Behavioural therapy in combination with stimulants seemed superior to stimulants or non-stimulants. Stimulants seemed superior to behavioural therapy, cognitive training and non-stimulants. Behavioural therapy, stimulants and their combination showed the best profile of acceptability. Stimulants and non-stimulants seemed well tolerated. Among medications, methylphenidate, amphetamine, atomoxetine, guanfacine and clonidine seemed significantly more efficacious than placebo. Methylphenidate and amphetamine seemed more efficacious than atomoxetine and guanfacine. Methylphenidate and clonidine seemed better accepted than placebo and atomoxetine. Most of the efficacious pharmacological treatments were associated with harms (anorexia, weight loss and insomnia), but an increased risk of serious adverse events was not observed. There is lack of evidence for cognitive training, neurofeedback, antidepressants, antipsychotics, dietary therapy, fatty acids, and other complementary and alternative medicine. Overall findings were limited by the clinical and methodological heterogeneity, small sample sizes of trials, short-term follow-up, and the absence of high-quality evidence; consequently, results should be interpreted with caution.<h4>Conclusions</h4>Clinical differences may exist between the pharmacological and non-pharmacological treatment used for the management of ADHD. Uncertainties about therapies and the balance between benefits, costs and potential harms should be considered before starting treatment. There is an urgent need for high-quality randomised trials of the multiple treatments for ADHD in children and adolescents. PROSPERO, number CRD42014015008.

Eswaradass Prasanna Venkatesan, Balakrishnan Ramasamy, Ramadoss Kalidoss et al.