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S2 Open Access 2020
Composition, Structure, and Digestive Dynamics of Milk From Different Species—A Review

Debashree Roy, A. Ye, P. Moughan et al.

Background: The traditional dairy-cattle-based industry is becoming increasingly diversified with milk and milk products from non-cattle dairy species. The interest in non-cattle milks has increased because there have been several anecdotal reports about the nutritional benefits of these milks and reports both of individuals tolerating and digesting some non-cattle milks better than cattle milk and of certain characteristics that non-cattle milks are thought to share in common with human milk. Thus, non-cattle milks are considered to have potential applications in infant, children, and elderly nutrition for the development of specialized products with better nutritional profiles. However, there is very little scientific information and understanding about the digestion behavior of non-cattle milks. Scope and Approach: The general properties of some non-cattle milks, in comparison with human and cattle milks, particularly focusing on their protein profile, fat composition, hypoallergenic potential, and digestibility, are reviewed. The coagulation behaviors of different milks in the stomach and their impact on the rates of protein and fat digestion are reviewed in detail. Key findings and Conclusions: Milk from different species vary in composition, structure, and physicochemical properties. This may be a key factor in their different digestion behaviors. The curds formed in the stomach during the gastric digestion of some non-cattle milks are considered to be relatively softer than those formed from cattle milk, which is thought to contribute to the degree to which non-cattle milks can be easily digested or tolerated. The rates of protein and fat delivery to the small intestine are likely to be a function of the macro- and micro-structure of the curd formed in the stomach, which in turn is affected by factors such as casein composition, fat globule and casein micelle size distribution, and protein-to-fat ratio. However, as no information on the coagulation behavior of non-cattle milks in the human stomach is available, in-depth scientific studies are needed in order to understand the impact of compositional and structural differences on the digestive dynamics of milk from different species.

251 sitasi en Chemistry, Medicine
DOAJ Open Access 2025
Feed additives for methane mitigation: A guideline to uncover the mode of action of antimethanogenic feed additives for ruminants

Alejandro Belanche, André Bannink, Jan Dijkstra et al.

ABSTRACT: This publication aims to provide guidelines of the knowledge required and the potential research to be conducted in order to understand the mode of action of antimethanogenic feed additives (AMFA). In the first part of the paper, we classify AMFA into 4 categories according to their mode of action: (1) lowering dihydrogen (H2) production; (2) inhibiting methanogens; (3) promoting alternative H2-incorporating pathways; and (4) oxidizing methane (CH4). The second part of the paper presents questions that guide the research to identify the mode of action of an AMFA on the rumen CH4 production from 5 different perspectives: (1) microbiology; (2) cell and molecular biochemistry; (3) microbial ecology; (4) animal metabolism; and (5) cross-cutting aspects. Recommendations are provided to address various research questions within each perspective, along with examples of how aspects of the mode of action of AMFA have been elucidated before. In summary, this paper offers timely and comprehensive guidelines to better understand and reveal the mode of action of current and emerging AMFA.

Dairy processing. Dairy products, Dairying
DOAJ Open Access 2025
Facilities and housing, husbandry, and health management practices on Quebec dairy farms: A retrospective descriptive study

Heidi Jim, Eduardo S. Ribeiro, Bruna Mion et al.

ABSTRACT: The objective of this retrospective descriptive study was to characterize housing and herd management practices of Quebec dairy farms. Pre-existent survey data (housing, husbandry, and herd health; 36 questions) collected in person by Lactanet technicians (n = 116; March 2020 to February 2021) from 1,965 herds were used. Results were segregated by facility type (freestall [FS] vs. tiestall herds [TS]) and summarized using descriptive statistics. The average herd size was 76 ± 56 cows, with 65 ± 47 milking cows, peaking at 40 ± 5 kg/d, with most herds housed their milking cows in TS facilities (80%). Of the FS herds (20%), 36% transitioned from TS after 2016. Based on the Canadian Code of Practice and peer-reviewed literature, management strengths included frequent bedding in TS, feed reduction before dry-off (>59% FS; >75% TS), routine hoof trimming (≥2×/yr in >80%), and adequate lighting (>85% maintained >200 lx for 14–17 h/d). Areas needed improvement included the adoption of secondary ventilation systems (observed in <45% FS and <20% TS), implementation of targeted dry-off protocols (>80% of herds applied a single dry-off protocol, regardless of milk yield) and greater adoption of teat sealant use (45% reported using intramammary antibiotics without teat sealants). Deep-bedded lying surfaces were uncommon (30% of far-off and lactating groups in FS; <20% of dry and lactating groups in TS). In FS herds, horizontal bars were most frequent in lactating groups (40%). In TS herds, 58% of herds calved cows in tiestalls and >80% lacked pasture or exercise pens access. Regarding hoof health, footbaths and sprays were largely absent in TS herds (90%), whereas FS herds more often used footbaths, especially for lactating cows (70%). These findings establish benchmarks for Quebec dairy herds, highlighting well-adopted practices and identifying opportunities to increase the uptake of specific management practices across the province to further enhance herd health and welfare.

Dairy processing. Dairy products, Dairying
DOAJ Open Access 2024
Streptococcus lutetiensis and Streptococcus equinus as potential emerging bovine mastitis pathogens

Bruna L. Crippa, Marjory X. Rodrigues, Tiago Tomazi et al.

ABSTRACT: The current study characterizes the genetic distribution of virulence and antimicrobial resistance of Streptococcus lutetiensis and Streptococcus equinus isolated from cows with clinical mastitis using whole genome sequencing (WGS). Although they are not the protagonist species within the genus Streptococcus, recent studies have isolated these species associated with bovine mastitis. In addition, these species are reported and isolated from humans and other animals. A total of four strains of S. lutetiensis and one of S. equinus were isolated from five cows with identified cases of clinical mastitis at a dairy farm near Ithaca, New York. Nineteen genes associated with antimicrobial resistance and 20 genes associated with virulence were identified in the analyzed strains. All strains presented genes associated with resistance: alr, ddl, gdpD, kasA, murA, lsa(E), msr(D), mef(A), gidB, and LiaF. Resistance genes associated with several different classes of antibiotics have also been reported. Sixteen virulence-associated genes were identified in all strains. Based on our findings, we conclude that the studied species have the potential to cause mastitis in cattle, and further studies are important to elucidate their role.

Veterinary medicine
DOAJ Open Access 2024
Unlocking Local and Regional Development through Nature-Based Tourism: Exploring the Potential of Agroforestry and Regenerative Livestock Farming in Mexico

Daniel Alfredo Revollo-Fernández, Debora Lithgow, Juan José Von Thaden et al.

Nature-based tourism offers several positive effects, including bringing tourists closer to nature and increasing environmental awareness among them, creating new sources of employment, diversifying local and regional economies, promoting the conservation of local ecosystems, and protecting biodiversity. A pilot exercise based on choice experiments is presented to estimate the monetary value per year of nature-based tourism (NbT). The exercise was applied in the Jamapa watershed in Mexico, and the results showed that NbT would amount to USD 7.7 million, with tourism activities linked to agroforestry and USD 5.5 million around regenerative cattle ranching. These results provide input for decision makers in developing public policies to benefit society, nature, and sustainable development.

Economics as a science
DOAJ Open Access 2023
The Value of Current <i>Ante Mortem</i> Meat Inspection and Food Chain Information of Dairy Cows in Relation to <i>Post Mortem</i> Findings and the Protection of Public Health: A Case for a More Risk-Based Meat Inspection

Pieter Jacobs, Boyd Berends, Len Lipman

In this study, the contribution of the <i>ante mortem</i> (AM) inspection and the food chain information (FCI) to ensuring meat safety and public health was investigated, by evaluating the slaughterhouse findings of 223,600 slaughtered dairy cows in the Netherlands. The outcome of this study was that the <i>ante mortem</i> (AM) and <i>post mortem</i> (PM) inspections have a substantial overlap, and that with regard to food safety and public health in over 99% of cases the PM could even be omitted on the basis of the AM. In this study, the data provided by the dairy farmers on the current FCI forms contributed little to nothing with regard to the outcomes of AM and PM inspection. It is concluded that current meat inspection procedures need an update and a more risk-based approach needs to be adopted. Regarding this, the AM inspection of dairy cattle should remain, because it plays an important role in ensuring food safety (e.g., by preventing contamination of the slaughter line by excessively dirty animals, or animals with abscesses), monitoring animal welfare and in detecting some important notifiable diseases. The PM inspection, however, could in many cases be omitted, provided there is a strict AM inspection complemented by a vastly improved (automated) way of obtaining reliable FCI.

Chemical technology
DOAJ Open Access 2022
Strategies for Reducing Ruminant Methane Emissions

Sheida E. V., Miroshnikov S. A., Duskaev G. K. et al.

The paper studies the effect of additional administration of ultrafine particles on the cattle rumen microbiome composition. The in vitro method was used using the ANKOM Daisy II incubator according to a specialized method. Microflora analysis was performed using MiSeq (Illumina, USA) by a new generation sequencing method with a MiSeq reagent kit. After a detailed analysis of the structure and composition of the microbial community in the contents of the rumen sampled for different diets, it was found that no significant differences were observed in the bacterial communities, with the exception of a slight shift in the Bacteroidetes/Firmicutes ratio. However, we observed numerical differences in the abundance of some representatives, namely, with additional inclusion of Fe and Cr2O3, decrease in the abundance of the methane-forming species Methanobrevibacter, Methanobacterium, Methanosphaera, and Methnaomicrobium was noted regarding the control.

Microbiology, Physiology
DOAJ Open Access 2021
Characterization of Fluorescent Dissolved Organic Matter in an Affected Pollution Raw Water Source using an Excitation-Emission Matrix and PARAFAC

Mohammad Rangga Sururi, Mila Dirgawati, Dwina Roosmini et al.

Cikapundung River is the main raw water source for 2-millions inhabitants of Bandung city but has been severely deteriorated due to organic pollution such as cattle manure, domestic, and agriculture wastes. Dissolved Organic Matter (DOM) in raw water can influence the process of water treatment. This study characterized and identified the origins of fluorescent DOM (FDOM) in Cikapundung River. Raw water samples were collected from intake outlets during dry and rainy seasons and analyzed using Fluorescence Excitation Emission Matrix spectroscopy combined with parallel factor (PARAFAC). FDOM origins were identified by Fluorescence-Index (FI) while autochthonous process contribution in water body was determined by Biological-Index (BIX). Chromophoric DOM as UV absorbance at 254 nm (A254) and Chemical Oxygen Demand (COD) were also measured. The FI were 1.82 (dry season) and 1.77 (rainy season), and the BIX were 0.92 (dry season) and 0.65 (rainy season). PARAFAC identified three compounds: water contaminant-like (C1), humic-like (C2) and tryptophan-like (C3) compounds. C2 was predominantly present in the rainy season with a C3/C2 ratio of 0.33. In the dry season, C3 increased substantially with a C3/C2 of 1.60. Strong correlation between C1 and C3 (R=0.86) was evidence that contaminant-like and tryptophan-like compounds were from the same anthropogenic sources. Strong correlation with A254 may indicate these identified compounds are aromatics.

Environmental technology. Sanitary engineering, Environmental sciences
DOAJ Open Access 2021
Isolation, Genomic Analysis, and Preliminary Application of a Bovine Klebsiella pneumoniae Bacteriophage vB_Kpn_B01

Zidan Luo, Shangjingchao Geng, Biao Lu et al.

Klebsiella pneumoniae is an important pathogen that can infect both humans and cattle. The widespread K. pneumoniae and its high drug resistance make it difficult to treat Klebsiella infections/diseases. In this study, a lytic K. pneumoniae bacteriophage vB_Kpn_B01 was isolated from a dairy farm trough in Sichuan Province, and its biological properties were studied, and the entire genome of vB_Kpn_B01 was sequenced. The therapeutic effects of the phage on disease-causing mice were preliminarily tested. Phages found in this study are double-stranded DNA bacterial viruses belonging to the family Siphoviridae, Sugarlandvirus. The results suggest that vB_Kpn_B01 has strong specificity and low adaptability to different adverse conditions. Meanwhile, the predicted gene products of phage vB_Kpn_B01 comprised 149 coding sequences (CDS) and 25 tRNAs, of which 34 CDS had known functions. Of course, vB_Kpn_B01 did not contain any known antibiotic-resistant or virulent genes. The pathological sections of the liver and lungs of mice showed that the inflammatory scores of the treatment group were lower than in the bacterial group. Phage vB_Kpn_B01 alleviated the inflammatory response in the organs of the infected mice, and the organ tissue bacterial load of the treatment group was significantly lower than that of the bacterial group. Therefore, vB_Kpn_B01 can inhibit the proliferation of K. pneumoniae 18 in vivo and can alleviate the inflammation of target organs caused by infectious bacteria, which preliminarily indicates that vB_Kpn_B01 has a certain therapeutic effect on laboratory-infected mice.

Veterinary medicine
DOAJ Open Access 2020
Cryopreservation of in vitro-produced bovine embryos by vitrification: In pursuit of a simplified, standardized procedure that improves pregnancy rates to promote cattle industry use

van Huong Do, Taylor-Robinson Andrew W.

The goal of cryopreservation is to retain the original stage of gametes and embryos after they have endured cooling and warming. Slow freezing is a standard method for in vivo-derived bovine embryo cryopreservation, three-fifths of such embryos being frozen by this method globally. However, it is evident that slow freezing is not efficient for cryopreserving in vitro-produced bovine embryos. Hence, only one-third of in vitro-produced bovine embryos are cryopreserved. Vitrification is a preferred method for storage of human embryos; consequently, it has been explored as a novel means to store in vitro-produced bovine embryos, for which it shows considerable promise as an alternative to slow freezing. This is due to several reasons: vitrification is often less time-consuming than slow freezing; it does not need expensive slow rate freezing machines; and it has been proven to have comparatively higher survival rates. Yet, in the cattle industry vitrification continues to present shortcomings, such as possible toxicity of vitrification solutions and failure to standardize methods, which pose a challenge for its application to in vitro-produced bovine embryos. Therefore, determining the most suitable procedure is crucial to make vitrification more practical in commercial settings.

Animal culture

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