Introduction: Hand hygiene is a cornerstone of infection prevention; however, the water and infrastructure used for handwashing can themselves become sources of microbial contamination. This study assessed the water quality and sanitary conditions of hand hygiene facilities in public and private hospitals in Addis Ababa, Ethiopia. Methods: A laboratory-based cross-sectional study was conducted from May to June 2025 in 10 hospitals. Forty samples were collected, including 20 tap water samples and 20 swab samples from tap handles. A sanitary survey evaluated water supply and hand hygiene stations, with onsite physicochemical measurements, and targeted microbial analyses performed. Data were recorded in an Excel spreadsheet and analyzed using SPSS, employing descriptive statistics and Chi-square tests, with significance defined at P < .05. Results: The sanitary survey showed that 62.5% of hospitals had continuous running water, with 75% of water systems free from leaks. Handwashing stations were accessible in 55% of facilities, primarily hand-operated (67.5%), followed by elbow-operated (25%) and foot-operated (7.5%). Maintenance deficiencies were noted in 37.5% of stations, with soap available at only 55%. Hand drying materials were present in 12.5% of hospitals, and only 45% of facilities reported regular cleaning of handwashing stations. Physicochemical water quality parameters were generally within acceptable limits. Microbiological analysis revealed high contamination: Escherichia coli was detected in 66.7% of all samples, found on 100% of tap handles compared to 31.6% of water samples ( P < .001). Other pathogens included Staphylococcus aureus (55%), Klebsiella (52.5%), Shigella (50%), and Salmonella (50%), all significantly prevalent on swabs than water samples ( P < .001), with no significant difference by hospital ownership. Conclusion: The study found significant microbial contamination in handwashing infrastructure. To reduce risks, facilities should install non-hand-operated stations, enhance routine cleaning and disinfection, and ensure proper maintenance. Regular water quality monitoring and infection control training are essential.
Environmental sciences, Public aspects of medicine
Abstract Background Salt Overly Sensitive 1 (SOS1), a plasma membrane Na+/H+ exchanger, is essential for plant salt tolerance. Salt damage is a significant abiotic stress that impacts plant species globally. All living organisms require copper (Cu), a necessary micronutrient and a protein cofactor for many biological and physiological processes. High Cu concentrations, however, may result in pollution that inhibits the growth and development of plants. The function and production of mangrove ecosystems are significantly impacted by rising salinity and copper contamination. Results A genome-wide analysis and bioinformatics techniques were used in this study to identify 20 SOS1 genes in the genome of Kandelia obovata. Most of the SOS1 genes were found on the plasma membrane and dispersed over 11 of the 18 chromosomes. Based on phylogenetic analysis, KoSOS1s can be categorized into four groups, similar to Solanum tuberosum. Kandelia obovata's SOS1 gene family expanded due to tandem and segmental duplication. These SOS1 homologs shared similar protein structures, according to the results of the conserved motif analysis. The coding regions of 20 KoSOS1 genes consist of amino acids ranging from 466 to 1221, while the exons include amino acids ranging from 3 to 23. In addition, we found that the 2.0 kb upstream promoter region of the KoSOS1s gene contains several cis-elements associated with phytohormones and stress responses. According to the expression experiments, seven randomly chosen genes experienced up- and down-regulation of their expression levels in response to copper (CuCl2) and salt stressors. Conclusions For the first time, this work systematically identified SOS1 genes in Kandelia obovata. Our investigations also encompassed physicochemical properties, evolution, and expression patterns, thereby furnishing a theoretical framework for subsequent research endeavours aimed at functionally characterizing the Kandelia obovata SOS1 genes throughout the life cycle of plants.
Walking is an indispensable mode of transportation for human survival. Gait is a characteristic of walking. In the clinic, patients with different diseases exhibit different gait characteristics. Gait analysis describes the specific situation of human gait abnormalities by observing and studying the kinematics and dynamics of limbs and joints during human walking and depicting the corresponding geometric curves and values. In foot and ankle diseases, gait analysis can evaluate the degree and nature of gait abnormalities in patients and provide an important basis for the diagnosis of patients’ diseases, the correction of abnormal gait and related treatment methods. This article reviews the relevant literature, expounds on the clinical consensus on gait, and summarizes the gait characteristics of patients with common ankle and foot diseases. Starting from the gait characteristics of individuals with different diseases, we hope to provide support and reference for the diagnosis, treatment and rehabilitation of clinically related diseases.
The current research investigated the use of gelatin nanoparticles (GNPs) for enhancing the cytotoxic effects of nivolumab, an immune checkpoint inhibitor. The unique feature of GNPs is their biocompatibility and functionalization potential, improving the delivery and the efficacy of immunotherapeutic drugs with fewer side effects compared to traditional treatments. This exploration of GNPs represents an innovative direction in the advancement of nanomedicine in oncology. Nivolumab-loaded GNPs were prepared and characterized. The optimum formulation had a particle size of 191.9 ± 0.67 nm, a polydispersity index of 0.027 ± 0.02, and drug entrapment of 54.67 ± 3.51%. A co-culture experiment involving A549 target cells and effector Jurkat cells treated with free nivolumab solution, and nivolumab-loaded GNPs, demonstrated that the latter had significant improvements in inhibition rate by scoring 87.88 ± 2.47% for drug-loaded GNPs against 60.53 ± 3.96% for the free nivolumab solution. The nivolumab-loaded GNPs had a lower IC<sub>50</sub> value, of 0.41 ± 0.01 µM, compared to free nivolumab solution (1.22 ± 0.37 µM) at 72 h. The results indicate that administering nivolumab-loaded GNPs augmented the cytotoxicity against A549 cells by enhancing effector Jurkat cell activity compared to nivolumab solution treatment.
Yu-Chieh Chou, Ting-Hsuan Sung, Shi-Jing Hou
et al.
<i>Cordyceps militaris</i> is a valuable medicinal fungus which has been widely used as a traditional medicine in East Asia. Compared to the well-known medicinal fungus <i>C. sinensis</i>, <i>C. militaris</i> can produce similar fermented metabolites with various biological activities, but it requires a shorter culture time and simpler culture conditions, and therefore, it has attracted increasing attention in recent years. The purpose of this review was to organize the current studies regarding metabolite production from <i>C. militaris</i> relative to their biological functions. We combined findings of metabolite production to correlate with different fermentation modes to obtain a full view of production processes used to yield the product. While research on <i>C. militaris</i> fermentation is not uncommon to date, its high value still highlights the importance of developing more modern fermentation processes for industrial production.
S. Barbuto Ferraiuolo, M. Cammarota, C. Schiraldi
et al.
Streptomyces is one of the most versatile genera for biotechnological applications, widely employed as platform in the production of drugs. Although streptomycetes have a complex life cycle and metabolism that would need multidisciplinary approaches, review papers have generally reported only studies on single aspects like the isolation of new strains and metabolites, morphology investigations, and genetic or metabolic studies. Besides, even if streptomycetes are extensively used in industry, very few review papers have focused their attention on the technical aspects of biotechnological processes of drug production and bioconversion and on the key parameters that have to be set up. This mini-review extensively illustrates the most innovative developments and progresses in biotechnological production and bioconversion processes of antibiotics, immunosuppressant, anticancer, steroidal drugs, and anthelmintic agents by streptomycetes, focusing on the process development aspects, describing the different approaches and technologies used in order to improve the production yields. The influence of nutrients and oxygen on streptomycetes metabolism, new fed-batch fermentation strategies, innovative precursor supplementation approaches, and specific bioreactor design as well as biotechnological strategies coupled with metabolic engineering and genetic tools for strain improvement is described. The use of whole, free, and immobilized cells on unusual supports was also reported for bioconversion processes of drugs. The most outstanding thirty investigations published in the last 8 years are here reported while future trends and perspectives of biotechnological research in the field have been illustrated. • Updated Streptomyces biotechnological processes for drug production are reported. • Innovative approaches for Streptomyces-based biotransformation of drugs are reviewed. • News about fermentation and genome systems to enhance secondary metabolite production.
In this study, we developed a highly sensitive and specific bimolecular fluorescence complementation (BiFC)-based influenza A virus (IAV)-sensing system by combining a galactose/glucose-binding protein (GGBP) with an N-terminal large domain (YN1-172) and a C-terminal small domain (YC173-239) made up of enhanced yellow fluorescence protein (eYFP). The GGBP-based BiFC reporter exhibits the fluorescence reconstitution as a result of conformational changes in GGBP when lactose, which was derived from 6′-silalyllactose and used as a substrate for neuraminidase (NA), binds to GGBP in the presence of IAV. The system showed a linear dynamic range extending from 1 × 10<sup>0</sup> to 1 × 10<sup>7</sup> TCID<sub>50</sub>/mL, and it had a detection limit of 1.1 × 10<sup>0</sup> TCID<sub>50</sub>/mL for IAV (H1N1), demonstrating ultra-high sensitivity. Our system exhibited fluorescence intensity enhancements in the presence of IAV, while it displayed weak fluorescence signals when exposed to NA-deficient viruses, such as RSV A, RSV B, adenovirus and rhinovirus, thereby indicating selective responses for IAV detection. Overall, our system provides a simple, highly sensitive and specific IAV detection platform based on BiFC that is capable of detecting ligand-induced protein conformational changes, obviating the need for virus culture or RNA extraction processes.
Zhen-Yang Shen, Zhen-Yang Shen, Yi-Feng Wang
et al.
Methionine is an essential sulfur-containing amino acid that finds widespread applications in agriculture, medicine, and the food industry. However, the complex and multibranched biosynthetic pathway of methionine has posed significant challenges to its efficient fermentation production. In this study, we employed a modularized synthetic biology strategy to improve the weakest branched pathway of methionine biosynthesis. Three exogenous modules were constructed and assembled to provide methyl donors, which are the primary limiting factors in methionine biosynthesis. The first module utilized added methanol, which was converted into 5,10-methylene-tetrahydrofolate for methionine production but was hindered by the toxicity of methanol. To circumvent this issue, a non-toxic formate module was constructed, resulting in a visible improvement in the methionine titer. Finally, an exogenous betaine module was constructed, which could directly deliver methyl to methionine. The final strain produced 2.87 g/L of methionine in a flask, representing a 20% increase over the starting strain. This study presents a novel strategy for improving and balancing other metabolites that are synthesized through complex multibranched pathways.
Abstract Public clinical trial registries contain a large amount of information about a large number of trials. Academic researchers have conducted various analyses using such data. However, some of these studies do not concern the medical condition or intervention that is the focus of each trial. We list examples of publications that have performed such analyses. Currently, there is no keyword to track relevant publications. Here, we propose a novel keyword, “Trial registry-metaresearch”, that could be used in such publications. This would be a great help to researchers who wish to more systematically search the literature for such metaresearch.
<i>Pedicularis kansuensis</i> is an indicator species of grassland degradation. Its population expansion dramatically impacts the production and service function of the grassland ecosystem, but the effects and mechanisms of the expansion are still unclear. In order to understand the ecological effects of <i>P. kansuensis</i>, three <i>P. kansuensis</i> patches of different densities were selected in an alpine grassland, and species diversity indexes, biomasses, soil physicochemical properties, and the mechanism among them were analyzed. The results showed that <i>P. kansuensis</i> expansion increased the richness index, the Shannon–Wiener index significantly, and the aboveground biomass ratio (ABR) of the Weed group (<i>p</i> < 0.05), but reduced the total biomass of the community and the ABR of the Gramineae and Cyperaceae decreased insignificantly (<i>p</i> > 0.05); soil moisture, soil AOC, and NO<sub>3</sub><sup>−</sup>·N decreased significantly (<i>p</i> < 0.05), while soil pH and total soil nutrients did not change significantly, and available phosphorus (AP) decreased at first and then increased (<i>p</i> < 0.05). The structural equation model (SEM) showed that <i>P. kansuensis</i> expansion had a significant positive effect on the community richness index, and a significant negative effect followed on the soil AOC from the increase of the index; the increase of pH had a significant negative effect on the soil AOC, NO<sub>3</sub><sup>−</sup>·N, and AP. It indicated that <i>P. kansuensis</i> expansion resulted in the increase of species richness, the ABR of the Weed group, and the community’s water demand, which promoted the over-utilization of soil available nutrients in turn, and finally caused the decline of soil quality. This study elucidated a possible mechanism of poisonous weeds expansion, and provided a scientific and theoretical basis for grassland management.
Oxidation has been a long sought-after alternative to classical thermal processing of oil shale, in order to obtain valuable raw materials for the chemical industry. A number of different methods have been applied, but thus far, one of the most effective ways to transform oil shale to value added products, such as aliphatic terminal dicarboxylic acids, is oxidation with nitric acid. In order to obtain insight into the reactivity of oil shale in nitric acid, a study focusing on the kinetics and behavior of oil shale particles during oxidative leaching was performed. To that end, the particle size distribution, surface area, and carbon content were measured during the leaching process in addition to the amount of total residual solids. Determining the carbon content of the solid residue was proposed as a simple measure of the reaction progress, based on the hypothesis that all carbon measured by elemental analysis correspond to organic carbon since inorganic carbon is present as carbonate in the starting material and would have dissolved under the acidic conditions. To our surprise, the solid residue had a significant amount of organic carbon in the form of calcium oxalate mineral. Thus, measuring carbon content in the solid residue could provide only an indirect measure of the overall oxidation degree provided that the amount of oxalates was known. In general, the results revealed that the total solid residue amounts to between 20% and 34% of the initial values after 24 h of the reaction, while the total carbon content ranges from 4% to 14% of the starting values. These results show that we were able to extract around 90% of the organic carbon present in the solid phase.
SUMMARY Keratin is a complex and structurally stable protein found in human and animal hard tissues, such as feathers, wool, hair, hoof and nails. Some of these, like feathers and wool, represent one of the main sources of protein-rich waste with significant potential to be transformed into value-added products such as feed, fertilizers or bioenergy. A major limitation impeding valorization of keratinous substrates is their recalcitrant structure and resistance to hydrolysis by common proteases. However, specialized keratinolytic enzymes produced by some microorganisms can efficiently degrade these substrates. Keratinases have already found a purpose in pharmaceutical, textile and leather industries. However, their wider implementation in other processes, such as cost-effective (pre)treatment of poultry waste, still requires optimization of production and performance of the available enzymes. Here we present a comprehensive review covering molecular properties and characteristics of keratinases, their classification, traditional and novel approaches in discovery of novel enzymes, production, characterization, improvement and biotechnological applications.