Hasil untuk "Dentistry"

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Maryam Ghaffari, Yi Zhu, Annie Shrestha

VANIA FONTANELLA, MARIA CELESTE MORITA

ABSTRACT The authors debate the arguments presented in the editorial “Reflections on the current context and evaluation of undergraduate medical education”, bringing the context that culminated in the recent application of the National Proficiency Exam in Dentistry.

Delia Goletti, Graeme Meintjes, Bruno B. Andrade et al.

Zhang Honghong, Kang Na

Aim or purpose: This study investigates the regulatory role of miR-23a-3p in the osteogenic differentiation of human periodontal ligament stem cells (HPDLSCs) by targeting LRP5, using lentivirus-mediated inhibition/overexpression models. Understanding this pathway may provide insights into periodontal bone remodeling and therapeutic strategies. Materials and methods: HPDLSCs were isolated from extracted premolars of orthodontic patients (12–20 years old) and characterized via morphological analysis, colony formation assays, and immunofluorescence staining. Lentiviral vectors were designed for miR-23a-3p overexpression/inhibition and LRP5 silencing. Transfection efficiency was determined by fluorescence microscopy and RT-qPCR. Osteogenic differentiation was induced, and the expression of LRP5, ALP, and RUNX2 was assessed by RT-qPCR and Western blot at multiple time points. Statistical significance was analyzed (p<0.05). Results: 1. LRP5 expression peaked on day 7 of osteogenic induction and declined thereafter (p<0.01). 2. miR-23a-3p overexpression suppressed LRP5, ALP, and RUNX2 expression, while its inhibition promoted osteogenic differentiation (p<0.01). LRP5 silencing reduced osteogenic differentiation, confirming its role in the process. 3. The ''miR-23a-3p inhibition + LRP5 silencing'' model demonstrated that miR-23a-3p negatively regulates osteogenesis by targeting LRP5. Conclusions: This study successfully established lentiviral gene modulation models and demonstrated that miR-23a-3p inhibits osteogenic differentiation of HPDLSCs via LRP5 suppression. These findings provide a potential molecular target for periodontal bone regeneration strategies.

Katayoun Katebi, Saba Yazdanian Asr, Zeinab Mahboobi et al.

Abstract Introduction Recurrent aphthous stomatitis (RAS) is one of the most prevalent oral inflammatory ulcerative lesions, characterized by painful ulcers that develop on non-keratinized oral mucosa, significantly affecting the quality of life. This study aimed to evaluate the prevalence of RAS and its associated risk factors within the Azar cohort population. Methods This cross-sectional study utilized data from the Azar cohort, which has been ongoing since 2014 in Shabestar City, East Azarbaijan, Iran, involving 15,006 adults aged 35 to 70 years. To assess the prevalence of RAS, participants were provided with a description of these lesions and asked whether they had ever experienced RAS in the oral cavity. Data collection was based on self-reports and examinations conducted by the physicians involved in the Azar cohort. Participants with RAS were classified into the RAS group, while the remaining participants were categorized into the non-RAS group. We assessed the association between RAS and various factors using binary logistic regression. Results In the study population, there were 3,503 individuals in the RAS group and 11,503 individuals in the non-RAS group. The prevalence of RAS in the Azar cohort was 23.34%. Individuals over 50 years of age (p < 0.001), those with a poor (p < 0.001) or very poor (p = 0.02) socio-economic status, a low educational level (p = 0.01), smokers (p < 0.001) and individuals with a history of smoking who have since quit (p = 0.01) were significantly less affected by RAS. Conversely, individuals with genital aphthous lesions (p < 0.001), depression (p < 0.001), rheumatoid disease (p = 0.01), and food allergies (p < 0.001) were significantly more affected by RAS. Conclusions Factors such as being under 50 years of age, possessing a high socioeconomic status, having a higher level of education, experiencing genital aphthous disease, suffering from depression, having rheumatoid disease, and having food allergies may be associated with a higher prevalence of RAS.

Myroslav Goncharuk-Khomyn

Frazão P, Paiato AP, Tavares ABS, Sampaio FC, Belotti L, Cury JA, Narvai PC, Grupo de Trabajo de la Asociación Latinoamericana de Odontopediatría ALOP

Se trata de una solicitud del Consejo Regional de Odontología de São Paulo (CROSP), organismo federal creado por la Ley N.º 4.324/1964, responsable de supervisar el ejercicio de las profesiones odontológicas y garantizar el cumplimiento de las normas éticas, para elaborar un Dictamen Técnico-Científico sobre la fluoración del agua de abastecimiento público, debido a la gran demanda que el organismo ha recibido sobre el tema, según consta en la solicitud realizada al Consejo Directivo de la Facultad de Salud Pública (FSP) de la Universidad de São Paulo (USP ), mediante CARTA PRES.0453/2020, de fecha 12/11/2020. En respuesta a la CROSP, el Director del FSP/USP autorizó, el 8/12/2020, la emisión del Dictamen antes mencionado. La Facultad de Salud Pública (FSP) de la Universidad de Sao Paulo (USP) tiene una larga trayectoria de colaboración con instituciones públicas y, de modo general, con la sosedad brasilera con respecto a la fluoración del agua para consumo humano, como una tecnología de salud pública para la prevención de caries dental. El 26 de noviembre de 2009, el Ministerio de Salud reconoció estos antecedentes, que se habían iniciado a mediados del siglo XX, y formalizó la instalación del Centro Colaborados en Vigilancia de Salud Bucal (CECOL) de la FSP/USP. Es así como entre las actividades de supervisión de salud bucal relacionadas al CECOL/USP se enfatizan los aspectos relacionados con la vigilancia de la fluoración del agua de abastecimiento público y temas asociados, con el objetivo de contribuir para mejorar la calidad de la gestión del sector y dotar al país de un sistema de vigilancia de la fluoración del agua que esté basado en informaciones confiables, buscando mejorar la calidad de los datos disponibles acerca de la cobertura de la fluoración en Brasil.

Fatemeh Abdolalian, Mojtaba Bayani, Saeid Afzali et al.

Abstract Background Periostin, a secreted adhesion molecule, is a matricellular protein secreted most in periodontal ligament and periosteum. Periostin is also needed for integrity and maturation of periodontal tissue. This meta-analysis was conducted to compare the gingival crevicular fluid (GCF) periostin levels in subjects having periodontal disease and healthy periodontium. Methods In this meta-analysis, three international database including PubMed, Scopus and Web of Science were searched and 207 studies retrieved. Also, the Google Scholar was searched to find more related studies (two studies were found). To assess the risk of bias of included studies, the Newcastle–Ottawa assessment scale adapted for case–control was used. Finally, required data was extracted and included into analysis. All statistical analysis were done using Stata software. Results Eight studies were included in this meta-analysis. Results showed that GCF periostin level is significant lower in chronic periodontitis group compare to healthy people (the standardized mean difference (SMD) = -3.15, 95% CI = -4.45, -1.85, p < 0.001). The syntheses of studies shown a significant decrease in the periostin level of chronic periodontitis patients compared to the gingivitis patients (SMD = -1.50, 95%CI = -2.52, -0.49, P = 0.003), while the mean level of periostin between the gingivitis patients and healthy group has no significant difference (SMD = -0.88, 95%CI = -2.14, 0.38, P = 0.173). Conclusion The mean concentration of GCF periostin in people with chronic periodontitis significantly decreased compared to people with gingivitis and also compared to healthy people, while no significant difference was observed between the two groups with gingivitis and healthy people. Therefore, this marker may be used as a diagnostic criterion for the disease, which requires further studies.

Jocić Ivana, Daković Dragana, Kandolf-Sekulović Lidija et al.

Introduction. Mucous membrane pemphigoid (MMP) is a rare autoimmune, chronic inflammatory disease that affects mucous membranes, most commonly the eyes and mouth, with or without skin involvement. It is a complex disease with several complications, including scarring, especially on conjunctival mucosa, that can lead to visual loss. Case report. We report four patients (two men and two women) with MMP. In all patients, the disease started between seventy and eighty years of age. The diagnosis was confirmed based on clinical appearance, histology, direct and indirect immunofluorescence studies, indirect split skin technique, and enzyme-linked immunosorbent assay (ELISA) test. The majority of lesions were on the gums and buccal mucosa; one patient had laryngeal involvement and a lesion on the umbilicus. No ocular involvement and no malignancy were detected. Direct immunofluorescence tests revealed continuous linear IgG deposition in the basal membrane zone in two patients, and they were treated with oral nicotinamide and tetracycline hydrochloride. In two patients, we detected IgG along with IgA linear deposition; they received treatment with methylprednisolone. Complete remission was achieved in all patients. Conclusion. Early diagnosis and an adequate therapeutic approach are necessary for the MMP treatment in long-term disease control and reduction of disease-related complications.

Zoe Marshman, Helen Rodd

Nikolaos Kotsanos, Haim Sarnat, Kitae Park

An experimental study of the stress- strain response of the samples of endodontically treated teeth restored using various technologies under loading in vitro has been carried out. An experimental set for mechanical tests in vitro has been designed and tested. The influence of luting agents and adhesive systems in the root canal on the quality of endodontic posts retention as well as durability of restorations has been investigated.

Veselina Panayotova, Albena Merzdhanova, Diana A. Dobreva et al.

Background: A diet rich in seafood has been linked to a variety of health benefits. While worldwide overfishing results in declining fish stocks, the growing demand for alternative sources of marine lipids has been expected. Rapana venosa (veined Rapa whelk) has become valuable seafood with nutritional and economic importance in the Black Sea region. Purpose: The aim of the present study was to provide knowledge about biologically active lipids in Black Sea Rapana venosa, harvested in the region of Varna. Material/Methods: Lipid classes were separated and purified by column and thin-layer chromatography. The saponifiable lipid fraction was derivatized into fatty acid methyl esters (FAMEs) and analysed by gas chromatography–mass spectrometry (GC-MS). Non-saponifiable lipids were identified by high pressure liquid chromatography coupled with UV/Vis and fluorescence detectors (HPLC-UV-FL). Results: Rapana venosa was characterized by low lipid content (0.50 g.100g-1 ww) with beneficial PUFA/SFA and n-6/n-3 ratios and high content of vitamin D3 and astaxanthin. Lipids comprised mainly of polar lipids. Polyunsaturated fatty acids represented more than 50% of total fatty acids, most abundant being from the omega-3 series. Sum of EPA and DHA accounted at 40.8% of total fatty acids. Lipid quality indices indicated the good anti-atherogenic and atni-trombogenic properties (AI and TI < 1) of rapana meat. Conclusions: The study revealed that Rapana venosa from the Black Sea is a good source of high quality marine lipids and presents а high potential for developing functional foods and/or dietary supplements with beneficial health effects.

Bunga Hasna Adilah, Riana Wardani, Cucu Zubaedah

Pendahuluan: Kesehatan gigi dan mulut seseorang dapat dilihat dari status kebersihan gigi dan mulutnya. Status kebersihan gigi dan mulut dapat dinilai menggunakan Debris Index-Simplified (DI-S). Penyuluhan mengenai pemeliharaan kebersihan gigi dan mulut merupakan salah satu upaya untuk meningkatkan kemampuan seseorang dalam menjaga kebersihan rongga mulutnya. Tujuan penelitian adalah untuk mengetahui perbedaan status kebersihan gigi dan mulut pada siswa Pondok Pesantren Salafiyah Al-Majidiyah sebelum dan sesudah penyuluhan. Metode: Jenis penelitian adalah pra-eksperimen dengan rancangan one group pretest-posttest. Sampel penelitian adalah 48 responden yang berusia 12-15 tahun. Pemeriksaan DI-S pada responden sebelum dan sesudah penyuluhan dilakukan sebanyak tiga kali. Data yang didapatkan kemudian diuji menggunakan uji normalitas. Uji Wilcoxon dilakukan karena data berdistribusi tidak normal. Hasil: Perbedaan indeks debris yang signifikan (nilai p=0,00<0,05) pada siswa Pondok Pesantren Salafiyah Al-Majidiyah dengan penurunan nilai rata-rata dari 1,355 menjadi 0,5383 sesudah penyuluhan mengenai pemeliharaan kebersihan gigi dan mulut sebanyak tiga kali. Simpulan: Terdapat perbedaan status kebersihan gigi dan mulut pada siswa Pondok Pesantren Salafiyah Al-Majidiyah menjadi lebih baik sesudah diberikan penyuluhan dibandingkan dengan sebelum diberikan penyuluhan. Kata kunci: Indeks debris, penyuluhan, siswa pondok pesantren, status kebersihan gigi dan mulut.   ABSTRACT Introduction: An individual’s oral health can be seen from their oral hygiene status. Oral hygiene status can be assessed using a Debris Index-Simplified (DI-S). Counselling regarding the maintenance of oral hygiene is one of the efforts to improve individual ability to maintain the oral hygiene. The purpose of the study was to determine the differences in the oral hygiene status of Salafiyah Al-Majidiyah Islamic Boarding School students before and after oral health counselling. Methods: The research was pre-experimental with one group pretest-posttest design. The study sample was as much as 48 respondents aged 12 - 15-years-old. The DI-S examination was conducted on respondents before and after three times oral health counselling. The data obtained was then tested using the normality test. The Wilcoxon test was also performed because the data were not normally distributed. Result: There was a significant difference in the debris index (p-value = 0.00 < 0.05) of Salafiyah Al-Majidiyah Islamic Boarding School students, with a decrease in the average value from 1.355 to 0.5383 after three times counselling of oral hygiene maintenance. Conclusion: There was a significant increase in oral hygiene status of Salafiyah Al-Majidiyah Islamic Boarding School students after being given oral health counselling. Keywords: Debris Index-Simplified (DI-S), oral health counselling, Islamic Boarding School students, oral hygiene status.

Rifayatullah Afridi, Aneela Ambreen, Faizan Ali Janjua et al.

Refeeding syndrome is a rare but preventable complication of malnutrition management. This is the second case of refeeding syndrome being reported from Pakistan. Although many cases have been reported worldwide. It is a clear indication of lack of knowledge of the entity (Refeeding syndrome) in our doctor’s community. Refeeding syndrome presents with a mix picture of clinical manifestations and thus it is necessary to keep a regular check and balance of electrolyte disturbance especially serum phosphate levels before and after initiating nutrition irrespective of the route nutrition are given through. We are reporting a case of refeeding syndrome in a severely malnourished 5-year-old child which unfortunately died despite taking strict lifesaving actions.

Intekhab Islam, Gopu Sriram, Mingming Li et al.

Cellular therapy using stem cells in bone regeneration has gained increasing interest. Various studies suggest the clinical utility of osteoprogenitors-like mesenchymal stem cells in bone regeneration. However, limited availability of mesenchymal stem cells and conflicting evidence on their therapeutic efficacy limit their clinical application. Human embryonic stem cells (hESCs) are potentially an unlimited source of healthy and functional osteoprogenitors (OPs) that could be utilized for bone regenerative applications. However, limited ability to track hESC-derived progenies in vivo greatly hinders translational studies. Hence, in this study, we aimed to establish hESC-derived OPs (hESC-OPs) expressing green fluorescent protein (GFP) and to investigate their osteogenic differentiation potential in vitro. We fluorescently labelled H9-hESCs using a plasmid vector encoding GFP. The GFP-expressing hESCs were differentiated into hESC-OPs. The hESC-OPsGFP+ stably expressed high levels of GFP, CD73, CD90, and CD105. They possessed osteogenic differentiation potential in vitro as demonstrated by increased expression of COL1A1, RUNX2, OSTERIX, and OPG transcripts and mineralized nodules positive for Alizarin Red and immunocytochemical expression of osteocalcin, alkaline phosphatase, and collagen-I. In conclusion, we have demonstrated that fluorescently labelled hESC-OPs can maintain their GFP expression for the long term and their potential for osteogenic differentiation in vitro. In future, these fluorescently labelled hESC-OPs could be used for noninvasive assessment of bone regeneration, safety, and therapeutic efficacy.

I. Inajati, Raphael Tri Endra Untara

Affecification with mineral trioxide aggregate and care of intracoronal bleaching on the non vital discoloration maxillary left central incisor. Maxillary anterior teeth in children and adults often experience trauma. This later makes the dental pulp roots that are not completely formed face the necrosis and apical closure stop later causing the apex wide and open. The opened apex can be coped with the care of affecification. Mineral Trioxide Aggregate (MTA) is the best material of affecification used for the formation of apical barrier. The purpose of this case report was to report the achievement of the apical sealing using MTA in the non-vital permanent maxillary left central incisor with the opened apical due to the trauma. The complaints of male patients aged 20 years was about the broken and discoloured left upper front teeth and becomes a traumatic event since the age of 10 years due to a fall and hit the cement floor. A clinical examination of non-vital teeth used the fracture Ellis IV class with wide open apex and discoloration. The radiographic image showed a widely opened apex with large root canal and there was a periapical radiolucency. The treatment given was affecification with MTA followed obturation with gutta-percha and sealer AH 26. In the following week it was continued with intracoronal bleaching with the application of sodium perborate and 30% hydrogen peroxide. Before treatment, the teeth were brownish (C4) and after treatment it turned into yellowish white (B2). A week after the bleaching treatment was completed and the installation of fibre post was done, followed by giving the composite resin restorations class IV cavity. The 2-week control later showed no abnormalities. In conclusion, the affecification treatment with MTA can accelerate treatment with the formation of apical barrier that stimulates the healing and may be followed by obturation with guttap percha followed by doing intracoronal bleaching and final restoration.   ABSTRAK Gigi anterior rahang atas pada anak-anak maupun dewasa sering mengalami trauma. Akibatnya pulpa gigi yang akarnya belum terbentuk sempurna akan mengalami nekrosis, dan penutupan apeks terhenti yang menyebabkan apeks lebar dan terbuka. Apeks yang terbuka dapat diatasi dengan perawatan apeksifikasi. Mineral Trioxide Aggregate (MTA) adalah bahan pilihan terbaik yang dipakai sebagai bahan apeksifikasi untuk pembentukan apical barrier. Tujuan laporan kasus ini adalah untuk melaporkan keberhasilan penutupan apikal dengan menggunakan MTA pada gigi permanen insisivus sentralis kiri maksila non vital dengan apikal yang terbuka karena trauma. Pasien laki-laki usia 20 tahun dengan keluhan gigi depan atas kiri patah dan berubah warna. Kejadian trauma sejak usia 10 tahun karena jatuh dan terbentur lantai. Pemeriksaan klinis gigi non vital dengan fraktur Ellis kelas IV disertai apeks terbuka lebar dan diskolorasi. Pada gambaran radiografis menunjukkan apeks yang terbuka lebar dengan saluran akar besar serta terdapat radiolusensi periapikal. Perawatan yang dilakukan adalah apeksifikasi dengan MTA dilanjutkan obturasi dengan gutta percha dan sealer AH 26. Setelah seminggu kemudian dilakukan intracoronal bleaching dengan aplikasi sodium perborat dan hidrogen peroksida 30%. Sebelum perawatan, gigi berwarna kecoklatan (C4) setelah dilakukan perawatan menjadi warna putih kekuningan (B2). Seminggu setelah perawatan bleaching selesai kemudian dilakukan pemasangan pasak fiber, dilanjutkan dengan restorasi resin komposit kavitas kelas IV. Kontrol 2 minggu kemudian tidak menunjukkan adanya kelainan. Kesimpulan hasil perawatan apeksifikasi dengan MTA dapat mempercepat waktu perawatan dengan terbentuknya barier apikal yang merangsang penyembuhan dan dapat dilanjutkan dengan obturasi dengan gutta percha, kemudian dilakukan bleaching intracoronal bleaching dilanjutkan dengan restorasi akhir.

Mihai BURLIBAŞA, Viorel PERIEANU, Radu COSTEA et al.

Objective. The purpose of this study is to draw attention of dental medicine specialist’s team on risks they are exposed to, by modifying treatment plans in order to fit medical tourism programs. Material and method. In this study a questionnaire was applied to a number of 173 dentists with differend specializations, regarding the adaption of treatment options for patients from medical tourism programs and risks posed by changes they had applied. Results. The analysis of results shows that the treatment approach for medical tourists is mostly based on a multidisciplinary team. In terms of quality of the treatment it is distinguished the use of specialists focused on specific fields of dentistry, but we must not neglect the fact that the therapeutical act is adapted to a reduced treatment time. Conclusions. For most of professionals of dental tourism, the financial aspect comes first and not the ethical aspect of medical care.

Marília Pacífico Lucisano, Paulo Nelson-Filho, Lea Assed Bezerra Silva et al.

The aim of this paper is to present the clinical and radiological outcome of the treatment involving a delayed tooth replantation after an avulsed immature permanent incisor, with a follow-up of 1 year and 6 months. An 8-year-old boy was referred after dental trauma that occurred on the previous day. The permanent maxillary right central incisor (tooth 11) had been avulsed. The tooth was hand-held during endodontic therapy and an intracanal medication application with calcium hydroxide-based paste was performed. An apical plug with mineral trioxide aggregate (MTA) was introduced into the apical portion of the canal. When the avulsed tooth was replanted with digital pressure, a blood clot had formed within the socket, which moved the MTA apical plug about 2 mm inside of the root canal. These procedures developed apical revascularization, which promoted a successful endodontic outcome, evidenced by apical closure, slight increase in root length, and absence of signs of external root resorption, during a follow-up of 1 year and 6 months.