Mosquitoes transmit a number of serious diseases, including brain fever, chikungunya, dengue fever, filariasis, hemorrhagic fever, Japanese encephalitis, malaria, dengue, and Zika. Whereas the synthetic chemical insecticides are commonly used for mosquito control, and it caused physiological resistance to mosquito species and significant effects on humans and the environment. As a result, plant-based insecticides have emerged as an attractive one. Recent research has revealed that green-synthesized silver nanoparticles derived from plant extracts have significant larvicidal properties. In the scenario we focused on application of nanotechnology. Nanotechnology is an emerging field of science and technology, it has incredible potential in pest management, medicine, and other fields. In this regards current investigation focused on the larvicidal and pupicidal effect of green-synthesized silver nanoparticles using C. aromaticus and W. tinctoria on second, third, and fourth-instar and pupa of Aedes aegypti. The green synthesized silver nanoparticles characterized by UV-Vis spectroscopy, FTIR, XRD, and SEM. The 24-hour LC50 and LC90 values obtained from C. aromatics and W. tinctoria synthesized silver nanoparticles against Ae. aegypti second, third, and fourth instars and pupae. The results showed that green synthesized silver nanoparticles from C. aromaticus are more effective than W. tinctoria nanoparticles used.
Nestor González Roldán, Lars P. Lunding, Yukari Fujimoto
et al.
IntroductionGrass pollen triggers nearly 30% of bronchial allergic asthma cases. While most Q8 research focuses on pollen allergens, pollen lipids may also influence allergic reactions. Previous studies demonstrated that Timothy grass (TG, Phleum pratense) lipids, such as phytoprostanes, can activate immune cells, promoting pro-allergic responses. However, the role of water-insoluble pollen glycolipids in allergic airway inflammation remains unclear. Thus, this study aimed to isolate and characterize glycolipids from TG pollen and evaluate their bioactivity in allergic airway inflammation.MethodsLipids were extracted from the water-insoluble pollen fraction, separated by silica gel, and fractionated by HPLC. GC-MS, HR ESI-MS, and NMR confirmed the presence of di-galactosyldiacylglycerol (DGDG). The biological activity of fractions containing DGDG (DGDG-3 and DGDG-4) and synthetic DGDG variants was tested in vitro in murine and human cell systems and in vivo in mice.ResultsFraction 4 induced strong proliferation of murine NKT cells and upregulated CD69 expression in human NKT cells. Synthetic DGDG variants (DGDG-1, DGDG-2, and DGDG-3) with defined acylation profiles stimulated robust NKT-cell proliferation, with DGDG-2 and DGDG-3 increasing IL-13 production, one of the key Th2 cytokines. In vivo, only these variants caused lung inflammation marked by eosinophil infiltration but did not increase airway resistance.DiscussionThis study reveals for the first time the structure-dependent role of DGDG of TG pollen grains in immune cell recognition in the context of allergic inflammation. Our data may pave the way for therapies targeting lipid components in combination with protein allergens.
Martin G. Zhen, Kathleen L. May, Robert A. Gossage
The synthesis and characterisation (UV-Vis, IR, HRESI-MS, <sup>1</sup>H and <sup>13</sup>C NMR spectroscopies, electrochemistry) is reported of the novel title material (<b>1</b>: alternatively named <i>rac</i>-1-(2′-benzothiazolyl)-2-ferrocenyl-2-propanol): a rare example of a ferrocenyl-benzothiazole hybrid species. Compound <b>1</b> is produced by the low temperature reaction of acetylferrocene (<b>3</b>) with a solution of the methyl anion derived via the deprotonation of 2-methyl-1,3-benzothiazole. The yield of <b>1</b> is moderate (34%) after purification and is an air and thermally stable solid under ambient conditions. Attempts to sublime <b>1</b>, however, result in decomposition with one of the products being identified (NMR) as <b>3</b>. The spectroscopic features of <b>1</b> are presented. Attempts to obtain suitable crystalline material of <b>1</b> for a single crystal X-ray diffraction study were unfortunately unsuccessful. Compound <b>1</b> also does not form stable coordination complexes with various metal salts (e.g., Ni[2+], Co[2+], etc.) under the conditions tested.
A. Alhadhrami, Muhammad Zeshan, Hafiz Muhammad Tahir Farid
Lanthanum substitution on spinel Nano particles has a significant repercussion on the several features of materials. Sol–gel (auto-ignition) allowed for the substitution of the rare earth lanthanum ion (La3+) for spinel ferrite with composition SrLaxFe2-xO4 (where 0.00 [Formula: see text] x [Formula: see text] 0.10). As a result of the base’s presence, the systems structural, magnetic, electrical, and magnetic properties are all modified. X-ray diffraction (XRD) assessed the structural characteristics. Using X-ray diffraction, we could determine that each sample had cubic structures. Based on electrical investigation, it can be concluded that the synthesized ferrites are semiconductors, as their direct current (dc) resistivity reduces with increasing temperature. The Curie temperature (Tc) was lowered from 532 K to 462 K with the substitution of lanthanum ions. The values for activation energy increased from 0.24 eV to 0.38 eV when lanthanum is used as a substitute. The values of magnetization decreased from 50 eV to 27 eV as lanthanum content rose. We could see the gentleness of the material in M-H loops. All these parameters show that the prepared sample is a potential material for use in high-frequency applications.
Mirella Kanerva, Nguyen Minh Tue, Tatsuya Kunisue
et al.
The Atlantic salmon (Salmo salar) population in the Baltic Sea consists of wild and hatchery-reared fish that have been released into the sea to support salmon stocks. During feeding migration, salmon migrate to different parts of the Baltic Sea and are exposed to various biotic and abiotic stressors, such as organohalogen compounds (OHCs). The effects of salmon origin (wild or hatchery-reared), feeding area (Baltic Main Basin, Bothnian Sea, and Gulf of Finland), and OHC concentration on the differences in hepatic proteome of salmon were investigated. Multi-level analysis of the OHC concentration, transcriptome, proteome, and oxidative stress biomarkers measured from the same salmon individuals were performed to find the key variables (origin, feeding area, OHC concentrations, and oxidative stress) that best account for the differences in the transcriptome and proteome between the salmon groups. When comparing wild and hatchery-reared salmon, differences were found in xenobiotic and amino acid metabolism-related pathways. When comparing salmon from different feeding areas, the amino acid and carbohydrate metabolic pathways were notably different. Several proteins found in these pathways are correlated with the concentrations of polychlorinated biphenyls (PCBs). The multi-level analysis also revealed amino acid metabolic pathways in connection with PCBs and oxidative stress variables related to glutathione metabolism. Other pathways found in the multi-level analysis included genetic information processes related to ribosomes, signaling and cellular processes related to the cytoskeleton, and the immune system, which were connected mainly to the concentrations of Polychlorinated biphenyls and Dichlorodiphenyltrichloroethane and their metabolites. These results suggest that the hepatic proteome of salmon in the Baltic Sea, together with the transcriptome, is more affected by the OHC concentrations and oxidative stress of the feeding area than the origin of the salmon.
The target simulation of airplanes is an important research topic. It is particularly important to find the right balance between high performance and low cost. In order to balance the contradictions between realistic target simulations and controllable costs, the scientific formulation of the performance parameters of target simulation is the key to achieving high performance. This paper proposes an intelligent simulation technology based on RCS imaging simulation through the combination of 60° variation corner reflector and a Luneberg lens reflector. It is designed to simulate several important RCS characteristics of the aircraft. At the same time, the different RCS images are automatically shifted to the corresponding gear position to achieve the purpose of simulation, and the price is low and the performance is good. It can be used for the training of radar target searching.
Germán Pérez-Sánchez, Nicolas Schaeffer, Tamar L. Greaves
et al.
Solutions of surfactants exhibit remarkable features, such as a tunable amphiphilic character, which can further be varied for ionic surfactants through variations in their Coulombic interactions. These properties are very useful in many industrial applications such as in extraction, purification, and formulation processes, as detergents, wetting agents, or emulsifiers. Rather unexpectedly, the addition of tetrabutylammonium chloride ([N4,4,4,4]Cl) to solutions of the ionic surfactant of sodium dodecyl sulphate (SDS) results in the appearance of a phase transition above the lower critical solution temperature (LCST), a property usually associated with non-ionic surfactants. The aim of this study is to provide a detailed nanoscopic scenario on the interaction between SDS micelles and [N4,4,4,4]Cl moieties to better understand the nature of the LCST cloud point and how to confer it to a given ionic surfactant system. A coarse-grained molecular dynamics (CG-MD) computational framework, under the latest MARTINI 3.0 force field, was developed and validated using available literature data. The impact of [N4,4,4,4]Cl concentration in the phase of SDS micellar aqueous solutions was then characterized and compared using experimental results. Specifically, dynamic light scattering (DLS) measurements and small-angle X-ray scattering (SAXS) profiles were obtained at different [N4,4,4,4]+/[DS]- molar ratios (from 0.0 to 1.0) and compared with the CG-MD results. A good agreement between computer simulations and experimental findings was obtained, reinforcing the suitability of GC-MD to simulate complex phase behaviors. When the [N4,4,4,4]+/[DS]- molar ratio is < 0.5, a weak impact of the cation in the micellar distribution was found whereas for ratios > 0.5, the system yielded clusters of enclosed small [DS]- aggregates. Thus, the CG-MD simulations showed the formation of mixed [DS]- and [N4,4,4,4]+ aggregates with [N4,4,4,4]+ cations acting as a bridge between small [DS]- micelles. The CG-MD simulation framework developed in this work captured the role of [N4,4,4,4]+ in the micellar phase transition whilst improving the results obtained with preceding computer models for which the limitations on capturing SDS and [N4,4,4,4]Cl mixtures in aqueous solutions are also shown in detail.
This study measured the time it took to select a target moving along a circular trajectory with a computer mouse. The time was changed according to the speed of the target, the width of target and the distance from the starting point to the target. However, the effect of these independent variables on the dependent variable was different from what was expected. In the previous studies, it was assumed that the faster the moving target speed, the longer the target selection time, because increased target speed had the effect of narrowing the effective target width. However, as a result of the experiment, the target selection time was rather shortened when the moving speed of the target was increased. This may be because the subjects intend to speed up target selection while decreasing the accuracy of target selection in order to adapt to a fast-moving target. The modified Fitts’ model for the moving target selection time proposed in a previous study did not take these user responses into account. A more modified model is required to more accurately describe the selection time of moving target.
Jan T. Benthien, Martin Riegler, Nick Engehausen
et al.
Replacing greenhouse gas-intensive building materials with wood products from sustainable forestry contributes to the implementation of current climate conventions such as the Paris Agreement. Hardwood products, such as laminated veneer lumber made of beech (e.g., BauBuche), are an alternative to conventional building materials. For the application of wood products in the construction sector, a precise knowledge of the mechanical and physical properties is essential. Therefore, the aim of the present study was to investigate the sorption behavior and associated dimensional changes of the product BauBuche. This was done by applying a manual testing procedure (climatic chamber, balance and caliper) as well as a dynamic vapor sorption analyzer equipped with a camera. During initial moistening after production, due to the irreversible spring back (approximately 2 mm at 50 mm; i.e., 4%), Baubuche shows an extremely strong swelling in the radial direction. Once the maximum spring back is reached, Baubuche shows sorption behavior in the radial and tangential direction, which is comparable to that of solid beech wood in the radial direction. Consequently, the dimensional changes caused by moisture changes must be taken into account in the dimensioning of Baubuche components in order to avoid damage to building structures.
Chemicals: Manufacture, use, etc., Textile bleaching, dyeing, printing, etc.
Mayakrishnan Prabakaran, Ji-Hee Lee, Ateeque Ahmad
et al.
The phenolic compounds (PC) of soybeans (Glycine max (L.) Merrill) varies mainly based on factors like genetics, the environment, and also the food processing techniques used. The effect of storage time and temperature on the phenolic acids and isoflavones composition of raw soybean flour (RWSF) and roasted soybean flour (RSF) were analyzed using liquid chromatography-tandem mass spectrometry (LC-MS/MS). Based on the analysis results, 56 PC and free amino acids were detected in the RWSF and RSF. The total phenolic content (TPC) was 301.59 µg/g in the control RWSF and 257.47 µg/g in the control RSF. In the analysis, eight types of phenolic acids and one flavonoid group belonging to the isoflavone group were detected. When comparing storage conditions of RWSF, 24 and 48 weeks of storage showed higher concentration of phenolic acids. In RSF, the percentage of total acetyl glucosides was high, but the outcome was reversed after 2 weeks. This study had identified that the composition of PC in RWSF and RSF were affected when the storage temperature increased and the storage time lengthened.
Tobias A. Timmerscheidt, Poulumi Dey, Dimitri Bogdanovski
et al.
Since the addition of Al to high-Mn steels is known to reduce their sensitivity to hydrogen-induced delayed fracture, we investigate possible trapping effects connected to the presence of Al in the grain interior employing density-functional theory (DFT). The role of Al-based precipitates is also investigated to understand the relevance of short-range ordering effects. So-called E21-Fe3AlC κ-carbides are frequently observed in Fe-Mn-Al-C alloys. Since H tends to occupy the same positions as C in these precipitates, the interaction and competition between both interstitials is also investigated via DFT-based simulations. While the individual H–H/C–H chemical interactions are generally repulsive, the tendency of interstitials to increase the lattice parameter can yield a net increase of the trapping capability. An increased Mn content is shown to enhance H trapping due to attractive short-range interactions. Favorable short-range ordering is expected to occur at the interface between an Fe matrix and the E21-Fe3AlC κ-carbides, which is identified as a particularly attractive trapping site for H. At the same time, accumulation of H at sites of this type is observed to yield decohesion of this interface, thereby promoting fracture formation. The interplay of these effects, evident in the trapping energies at various locations and dependent on the H concentration, can be expressed mathematically, resulting in a term that describes the hydrogen embrittlement.
The first general procedure for the synthesis of 5 to 7-membered 1-aryl-2-iminoazacycloalkanes is presented, by microwave-assisted ring closure of ω-arylaminonitriles promoted by polyphosphoric acid (PPA) esters. 1-Aryl-2-iminopyrrolidines were easily prepared from the acyclic precursors employing a chloroformic solution of ethyl polyphosphate (PPE). The use of trimethylsilyl polyphosphate (PPSE) in solvent-free conditions allowed for the synthesis of 1-aryl-2-iminopiperidines and hitherto unreported 1-aryl-2-iminoazepanes. The cyclization reaction involves good to high yields and short reaction times, and represents a novel application of PPA esters in heterocyclic synthesis.
Successful biogas production is based on stable or adaptable microbial community structure and activity which depends on type of substrate used and several physico-chemical conditions in the bioreactor. Monitoring those and the dynamics of microbiota is important for planning and optimizing the biogas process, avoiding critical points and reaching the maximum methane yield. Methanogens are extremely difficult to study with culture-based methods. Molecular methods for microbial community structure analysis in biogas reactors, which offer qualitative and quantitative information on bacterial and archaeal species and their microbial community changes, and causes for process instability are surveyed in this review. For comparative studies semi-quantitative, rapid and cheap techniques like T-RFLP, DGGE and TGGE are used. More laborious and expensive techniques with high-throughput like semi-quantitative FISH and DNA microarrays and also quantitative techniques like qPCR and sequencing are used for phylogenetic analysis. Technique type adequacy for certain study depends on what information is needed and on several advantages and disadvantages every technique possesses.
Abstract A search for top quark pair resonances in final states containing at least one electron or muon has been performed with the ATLAS experiment at the CERN Large Hadron Collider. The search uses a data sample corresponding to an integrated luminosity of 2.05 fb−1, which was recorded in 2011 at a proton-proton centre-of-mass energy of 7 TeV. No evidence for a resonance is found and limits are set on the production cross-section times branching ratio to $t\bar{t}$ for narrow and wide resonances. For narrow Z′ bosons, the observed 95 % Bayesian credibility level limits range from 9.3 pb to 0.95 pb for masses in the range of m Z′=500 GeV to m Z′=1300 GeV. The corresponding excluded mass region for a leptophobic topcolour Z′ boson (Kaluza-Klein gluon excitation in the Randall-Sundrum model) is m Z′<880 GeV ( $m_{g_{\mathrm{KK}}} < 1130~ \mathrm{GeV}$ ).
Astrophysics, Nuclear and particle physics. Atomic energy. Radioactivity
An unusually strong and prolonged stratospheric sudden warming (SSW) in January 2006 was the first major SSW for which globally distributed long-lived trace gas data are available covering the upper troposphere through the lower mesosphere. We use Aura Microwave Limb Sounder (MLS), Atmospheric Chemistry Experiment-Fourier Transform Spectrometer (ACE-FTS) data, the SLIMCAT Chemistry Transport Model (CTM), and assimilated meteorological analyses to provide a comprehensive picture of transport during this event. The upper tropospheric ridge that triggered the SSW was associated with an elevated tropopause and layering in trace gas profiles in conjunction with stratospheric and tropospheric intrusions. Anomalous poleward transport (with corresponding quasi-isentropic troposphere-to-stratosphere exchange at the lowest levels studied) in the region over the ridge extended well into the lower stratosphere. In the middle and upper stratosphere, the breakdown of the polar vortex transport barrier was seen in a signature of rapid, widespread mixing in trace gases, including CO, H<sub>2</sub>O, CH<sub>4</sub> and N<sub>2</sub>O. The vortex broke down slightly later and more slowly in the lower than in the middle stratosphere. In the middle and lower stratosphere, small remnants with trace gas values characteristic of the pre-SSW vortex lingered through the weak and slow recovery of the vortex. The upper stratospheric vortex quickly reformed, and, as enhanced diabatic descent set in, CO descended into this strong vortex, echoing the fall vortex development. Trace gas evolution in the SLIMCAT CTM agrees well with that in the satellite trace gas data from the upper troposphere through the middle stratosphere. In the upper stratosphere and lower mesosphere, the SLIMCAT simulation does not capture the strong descent of mesospheric CO and H<sub>2</sub>O values into the reformed vortex; this poor CTM performance in the upper stratosphere and lower mesosphere results primarily from biases in the diabatic descent in assimilated analyses.