Hasil untuk "q-bio.OT"

F. H. Jackson

Amy Denton, Caroline Howard

This protocol is for the fragmentation of HMW DNA from either the MagAttract v.3, Nanobind tissue or Nanobind nucleated blood Sanger Tree of Life HMW DNA extraction protocols, using the Opentrons® OT-2 liquid handler. This process is highly effective for the fragmentation of DNA extracted from bird and fish species covered by the Tree of Life Programme, with DNA sheared into an average fragment size range of 30–70 kb for ONT sequencing. The output of this protocol is sheared DNA which can be directed towards the Sanger Tree of Life Fragmented DNA clean up: Automated SPRI v2 protocol. This protocol was adapted from settings used for pipette shearing HMW DNA on the Opentrons® Flex as described in ‘Fragmenting High Molecular Weight DNA for PacBio® Long-Read Sequencing Using Pipette Shearing’ by D. Howell et al. and from settings for pipette shearing HMW DNA on the Opentrons® OT-2 as detailed in the Sanger Tree of Life HMW DNA Fragmentation: Opentrons® OT-2 for PacBio LI protocol.

S. Cohn

R. McCrae, P. Costa, C. M. Busch

M. Dewood, W. F. Stifter, C. S. Simpson et al.

John J. Degnan

K. Folkers, P. Langsjoen, R. Willis et al.

J. Zhou, Q. Wang, Q. Sun et al.

K. Enqvist, J. McDonald

We show that Q-balls naturally exist in the Minimal Supersymmetric Standard Model (MSSM) with soft SUSY breaking terms of the minimal N = 1 SUGRA type. These are associated with the F-and D-flat directions of the scalar potential once radiative corrections are taken into account. We consider two distinct cases, corresponding to the "HuL" (slepton) direction with L-balls and the "u(c)d(c)d(c)" and "u(c)u(c)d(c)e(c)" (squark) directions with B-balls. The L-ball always has a small charge, typically of the order of 1000, whilst the B-ball can have an arbitrarily large charge, which, when created cosmologically by the collapse of an unstable Affleck-Dine condensate, is likely to be greater than 10(14). The B-balls typically decay at temperatures less than that of the electroweak phase transition, leading to a novel version of Affleck-Dine baryogenesis, in which the B asymmetry comes from Q-ball decay rather than condensate decay. This mechanism can work even in the presence of additional L violating interactions or B-L conservation, which would rule out conventional Affleck-Dine baryogenesis.

Wilbur G. Lewellen, S. Badrinath

Emmanouil Angelakis, D. Raoult

C. Castellano, M. A. Muñoz, R. Pastor-Satorras

We introduce a nonlinear variant of the voter model, the q-voter model, in which q neighbors (with possible repetition) are consulted for a voter to change opinion. If the q neighbors agree, the voter takes their opinion; if they do not have a unanimous opinion, still a voter can flip its state with probability epsilon . We solve the model on a fully connected network (i.e., in mean field) and compute the exit probability as well as the average time to reach consensus by employing the backward Fokker-Planck formalism and scaling arguments. We analyze the results in the perspective of a recently proposed Langevin equation aimed at describing generic phase transitions in systems with two ( Z2-symmetric) absorbing states. In particular, by deriving explicitly the coefficients of such a Langevin equation as a function of the microscopic flipping probabilities, we find that in mean field the q-voter model exhibits a disordered phase for high epsilon and an ordered one for low epsilon with three possible ways to go from one to the other: (i) a unique (generalized-voter-like) transition, (ii) a series of two consecutive transitions, one (Ising-like) in which the Z2 symmetry is broken and a separate one (in the directed-percolation class) in which the system falls into an absorbing state, and (iii) a series of two transitions, including an intermediate regime in which the final state depends on initial conditions. This third (so far unexplored) scenario, in which a type of ordering dynamics emerges, is rationalized and found to be specific of mean field, i.e., fluctuations are explicitly shown to wash it out in spatially extended systems.

S. More, J. Stegeman, A. Rodolakis et al.

Martin A. M. Reijns, Ross D. Alexander, M. P. Spiller et al.

P-bodies are cytoplasmic foci that are sites of mRNA degradation and translational repression. It is not known what causes the accumulation of RNA-degradation factors in P-bodies, although RNA is required. The yeast Lsm1-7p complex (comprising Lsm1p to Lsm7p) is recruited to P-bodies under certain stress conditions. It is required for efficient decapping and degradation of mRNAs, but not for the assembly of P-bodies. Here we show that the Lsm4p subunit and its asparagine-rich C-terminus are prone to aggregation, and that this tendency to aggregate promotes efficient accumulation of Lsm1-7p in P-bodies. The presence of glutamine- and/or asparagine-rich (Q/N-rich) regions in other P-body components suggests a more general role for aggregation-prone residues in P-body localization and assembly. This is supported by reduced P-body accumulation of Ccr4p, Pop2p and Dhh1p after deletion of these domains, and by the observed aggregation of the Q/N-rich region from Ccr4p.

Taekyun Kim

A. Aral, Vijay Gupta

In the present paper we propose a generalization of the Baskakov operators, based on q integers. We also estimate the rate of convergence in the weighted norm. In the last section, we study some shape preserving properties and the property of monotonicity of q-Baskakov operators.

R. Dasgupta, R. Clark

J. Afonso, C. Couto, J. S. Martins

Yoshinori Tanaka, J. Upham, T. Nagashima et al.