Hasil untuk "Biotechnology"

G. Pisano

M. Asadi, Saeed Taghizadeh, Elina Kaviani et al.

Caspase‐3, a cysteine‐aspartic acid protease, has recently attracted much attention because of its incredible roles in tissue differentiation, regeneration, and neural development. This enzyme is a key zymogen in cell apoptosis and is not activated until it is cleaved by initiator caspases during apoptotic flux. Since caspase‐3 has represented valuable capabilities in the field of medical research, biotechnological aspects of this enzyme, including the production of recombinant type, protein engineering, and designing delivery systems, have been considered as emerging therapeutic strategies in treating the apoptosis‐related disorders. To date, several advances have been made in the therapeutic use of caspase‐3 in the management of some diseases such as cancers, heart failure, and neurodegenerative disorders. In the current review, we intend to discuss the caspase‐3's structure, functions, therapeutic applications, as well as its molecular cloning, protein engineering, and relevant delivery systems.

J. Lerner

I. Raskin, D. Ribnicky, S. Komarnytsky et al.

F. Freitas, V. Alves, M. Reis

B. Spellberg, J. Powers, E. Brass et al.

R. Muller, C. Keck

A. Oren

A. I. Canas, S. Camarero

Ren Wei, T. Tiso, J. Bertling et al.

N. Brown

R. Reis, A. Zydney

W. Leuchtenberger, K. Huthmacher, K. Drauz

P. Metzger, C. Largeau

Fernando Pagels, A. Guedes, Helena M Amaro et al.

Phycobiliproteins are a group of water soluble proteins with an associated chromophore, responsible for the light-harvesting in cyanobacteria. They are divided in four main types: phycoerythrin, phycocyanin, phycoerythrocyanin and allophycocyanin, and they are characterized according to their structure and light quality absorption. Phycobiliproteins from cyanobacteria have been described as potential bioactive compounds, and recognized as high-valued natural products for biotechnological applications. Moreover, phycobiliproteins have been associated to antioxidant, anticancer and anti-inflammatory capacities among others. Thus, in order to produce phycobiliproteins from cyanobacteria for industrial application, it is necessary to optimize the whole bioprocess, including the processing parameters (such as light, nitrogen and carbon source, pH, temperature and salinity) that affects the growth and phycobiliprotein accumulation, as well as the optimization of phycobiliproteins extraction and purification. The aim of this review is to give an overview of phycobiliproteins not only in terms of their chemistry, but also in terms of their biotechnological applicability and the advances and challenges in the production of such compounds.

A. Pérez-García, D. Romero, A. de Vicente

J. A. Campo, M. García-González, M. Guerrero

D. Tan, Ying Wang, Yi Tong et al.

R. Riley, S. Haridas, K. H. Wolfe et al.

Rachel Cruz Alves, José Henrique Franscisco Roma, Bruno Moreira Carneiro et al.

Abstract RNA extraction is usually required for molecular detection methods. On the other hand, this step implies time consumption and additional cost to laboratorial routine. This study evaluated the use of direct-RT-qPCR for SARS-CoV-2 detection compared to the gold standard extraction method. An exploratory stage was performed using DMSO, glycerol, Tween 20 and Easy Extract® DNA-RNA addition in a pool of highly positive clinical samples (CT<25), which were subsequently tested by AllplexTM 2019-nCoV assay. Validation was performed with 54 SARS-CoV-2 positive and five negative clinical samples comparing three distinct conditions: (i) 1:1 water dilution, (ii) 1.5% DMSO dilution, or (iii) crude samples. Results of the exploratory stage showed Cycle Threshold (CT) values very close to those obtained by the reference extraction step in high viral load samples. However, when lower viral load samples were analyzed, a slight concordance was observed compared to the extraction step. In the validation stage, it was observed that water or 1.5% DMSO dilution presented 97.3% of concordance in samples with CT≤30, resulting in 97.4% of sensitivity and 0.83 Kappa coefficient. Sensitivity was reduced with samples CT>30 and with crude samples. The methodology showed reproducibility with differents operators. These results reinforce the good performance of direct RT-qPCR methods in active SARS-CoV-2 infection, allowed cost and time reduction of diagnosis.