Hasil untuk "Animal culture"

N. Gjorevski, Norman Sachs, Andrea Manfrin et al.

J. Henrich

D. Frank

J. Bonner

Animals do have culture, maintains this delightfully illustrated and provocative book, which cites a number of fascinating instances of animal communication and learning. John Bonner traces the origins of culture back to the early biological evolution of animals and provides examples of five categories of behavior leading to nonhuman culture: physical dexterity, relations with other species, auditory communication within a species, geographic locations, and inventions or innovations. Defining culture as the transmission of information by behavioral rather than genetical means, he demonstrates the continuum between the traits we find in animals and those we often consider uniquely human.

Jessica Hoarau-Véchot, A. Rafii, C. Touboul et al.

An area that has come to be of tremendous interest in tumor research in the last decade is the role of the microenvironment in the biology of neoplastic diseases. The tumor microenvironment (TME) comprises various cells that are collectively important for normal tissue homeostasis as well as tumor progression or regression. Seminal studies have demonstrated the role of the dialogue between cancer cells (at many sites) and the cellular component of the microenvironment in tumor progression, metastasis, and resistance to treatment. Using an appropriate system of microenvironment and tumor culture is the first step towards a better understanding of the complex interaction between cancer cells and their surroundings. Three-dimensional (3D) models have been widely described recently. However, while it is claimed that they can bridge the gap between in vitro and in vivo, it is sometimes hard to decipher their advantage or limitation compared to classical two-dimensional (2D) cultures, especially given the broad number of techniques used. We present here a comprehensive review of the different 3D methods developed recently, and, secondly, we discuss the pros and cons of 3D culture compared to 2D when studying interactions between cancer cells and their microenvironment.

Weiwei Lv, Wenzong Zhou, Shibo Lu et al.

Microplastics are emerging contaminants of increasing concern. Despite the occurrence of microplastics in farmland soils, the knowledge on microplastics in rice-fish co-culture ecosystems is limited. In this study, we investigated the distribution of microplastics in three rice-fish culture stations in Shanghai. During non-rice and rice-planting periods, microplastics in water, soils and aquatic animals (eel, loach and crayfish) were systematically assayed using methods of NaCl density extraction, H2O2 digestion and micro-fourier transform infrared spectroscopy. Results showed that average microplastic abundances were 0.4 ± 0.1 items L-1, 10.3 ± 2.2 items kg-1, 1.7 ± 0.5 items individual-1 in water, soils and aquatic animal samples, respectively. We found an increasing trend in microplastic abundances in water, soil and animal samples from non-rice period to rice-planting period. Almost all of microplastics were found in digestive tracts of animals. Major microplastics were small (<1 mm) polyethylene and polypropylene fibers, with color of white and translucent. Size, shape, color and polymer type distributions of microplastics were similarly found in environmental and animal samples. Moreover, microplastic abundances in aquatic animals correlated to abundance in farmland soils. This study, for the first time, reveals the occurrence and characteristics of microplastic pollution in rice-fish culture ecosystem which suggests the potential ecological risks of microplastics in the agroecosystem.

Donglai Lv, Zong-tao Hu, Lin Lu et al.

In previous years, three-dimensional (3D) cell culture technology has become a focus of research in tumor cell biology, using a variety of methods and materials to mimic the in vivo microenvironment of cultured tumor cells ex vivo. These 3D tumor cells have demonstrated numerous different characteristics compared with traditional two-dimensional (2D) culture. 3D cell culture provides a useful platform for further identifying the biological characteristics of tumor cells, particularly in the drug sensitivity area of the key points of translational medicine. It promises to be a bridge between traditional 2D culture and animal experiments, and is of great importance for further research in the field of tumor biology. In the present review, previous 3D cell culture applications, focusing on anti-tumor drug susceptibility testing, are summarized.

Thomas P. Keeley, G. Mann

The extensive oxygen gradient between the air we breathe (Po2 ~21 kPa) and its ultimate distribution within mitochondria (as low as ~0.5-1 kPa) is testament to the efforts expended in limiting its inherent toxicity. It has long been recognized that cell culture undertaken under room air conditions falls short of replicating this protection in vitro. Despite this, difficulty in accurately determining the appropriate O2 levels in which to culture cells, coupled with a lack of the technology to replicate and maintain a physiological O2 environment in vitro, has hindered addressing this issue thus far. In this review, we aim to address the current understanding of tissue Po2 distribution in vivo and summarize the attempts made to replicate these conditions in vitro. The state-of-the-art techniques employed to accurately determine O2 levels, as well as the issues associated with reproducing physiological O2 levels in vitro, are also critically reviewed. We aim to provide the framework for researchers to undertake cell culture under O2 levels relevant to specific tissues and organs. We envisage that this review will facilitate a paradigm shift, enabling translation of findings under physiological conditions in vitro to disease pathology and the design of novel therapeutics.

D. Ingber

For the past century, experimental data obtained from animal studies have been required by reviewers of scientific articles and grant applications to validate the physiological relevance of in vitro results. At the same time, pharmaceutical researchers and regulatory agencies recognize that results from preclinical animal models frequently fail to predict drug responses in humans. This Progress Report reviews recent advances in human organ‐on‐a‐chip (Organ Chip) microfluidic culture technology, both with single Organ Chips and fluidically coupled human “Body‐on‐Chips” platforms, which demonstrate their ability to recapitulate human physiology and disease states, as well as human patient responses to clinically relevant drug pharmacokinetic exposures, with higher fidelity than other in vitro models or animal studies. These findings raise the question of whether continuing to require results of animal testing for publication or grant funding still makes scientific or ethical sense, and if more physiologically relevant human Organ Chip models might better serve this purpose. This issue is addressed in this article in context of the history of the field, and advantages and disadvantages of Organ Chip approaches versus animal models are discussed that should be considered by the wider research community.

Takumi Ohashi, Hitoshi Iyatomi

Large language models (LLMs) are increasingly deployed in multicultural settings; however, systematic evaluation of cultural specificity at the sentence level remains underexplored. We propose the Conceptual Cultural Index (CCI), which estimates cultural specificity at the sentence level. CCI is defined as the difference between the generality estimate within the target culture and the average generality estimate across other cultures. This formulation enables users to operationally control the scope of culture via comparison settings and provides interpretability, since the score derives from the underlying generality estimates. We validate CCI on 400 sentences (200 culture-specific and 200 general), and the resulting score distribution exhibits the anticipated pattern: higher for culture-specific sentences and lower for general ones. For binary separability, CCI outperforms direct LLM scoring, yielding more than a 10-point improvement in AUC for models specialized to the target culture. Our code is available at https://github.com/IyatomiLab/CCI .

Binchi Zhang, Xujiang Zhao, Jundong Li et al.

Large language models (LLMs) are increasingly deployed in culturally sensitive real-world tasks. However, existing cultural alignment approaches fail to align LLMs' broad cultural values with the specific goals of downstream tasks and suffer from cross-culture interference. We propose CultureManager, a novel pipeline for task-specific cultural alignment. CultureManager synthesizes task-aware cultural data in line with target task formats, grounded in culturally relevant web search results. To prevent conflicts between cultural norms, it manages multi-culture knowledge learned in separate adapters with a culture router that selects the appropriate one to apply. Experiments across ten national cultures and culture-sensitive tasks show consistent improvements over prompt-based and fine-tuning baselines. Our results demonstrate the necessity of task adaptation and modular culture management for effective cultural alignment.

A. A. Shah, Pajaree Totakul, Maharach Matra et al.

The aim of the present investigation is to determine the nutritional composition of various insects and their potential uses as alternative protein sources in animal diets. The feeding industry requires production systems that use accessible resources, such as feed resources, and concentrates on the potential impacts on production yield and nutritional quality. Invertebrate insects, such as black soldier flies, grasshoppers, mealworms, housefly larvae, and crickets, have been used as human food and as feed for nonruminants and aqua culture while for ruminants their use has been limited. Insects can be mass-produced, participating in a circular economy that minimizes or eliminates food- and feed-waste through bioconversion. Although the model for formula-scale production of insects as feed for domestic animals has been explored for a number of years, significant production and transformation to being a conventional protein resource remains to be deeply investigated. This review will focus on the nutritional composition of various insects and their potential use as alternative protein sources, as well as their potential use to promote and support sustainable animal production. Furthermore, nutritional compositions, such as high protein, lauric acid omega 6, and omega 3, and bioactive compounds, such as chitin, are of great potential use for animal feeding.

Tabinda Aman, Mohammad Nadeem, Shahab Saquib Sohail et al.

Animal stereotypes are deeply embedded in human culture and language. They often shape our perceptions and expectations of various species. Our study investigates how animal stereotypes manifest in vision-language models during the task of image generation. Through targeted prompts, we explore whether DALL-E perpetuates stereotypical representations of animals, such as "owls as wise," "foxes as unfaithful," etc. Our findings reveal significant stereotyped instances where the model consistently generates images aligned with cultural biases. The current work is the first of its kind to examine animal stereotyping in vision-language models systematically and to highlight a critical yet underexplored dimension of bias in AI-generated visual content.

Naitian Zhou, David Bamman, Isaac L. Bleaman

The field of cultural NLP has recently experienced rapid growth, driven by a pressing need to ensure that language technologies are effective and safe across a pluralistic user base. This work has largely progressed without a shared conception of culture, instead choosing to rely on a wide array of cultural proxies. However, this leads to a number of recurring limitations: coarse national boundaries fail to capture nuanced differences that lay within them, limited coverage restricts datasets to only a subset of usually highly-represented cultures, and a lack of dynamicity results in static cultural benchmarks that do not change as culture evolves. In this position paper, we argue that these methodological limitations are symptomatic of a theoretical gap. We draw on a well-developed theory of culture from sociocultural linguistics to fill this gap by 1) demonstrating in a case study how it can clarify methodological constraints and affordances, 2) offering theoretically-motivated paths forward to achieving cultural competence, and 3) arguing that localization is a more useful framing for the goals of much current work in cultural NLP.

Daniel Mwesigwa

AI is flattening culture. Evaluations of "culture" are showing the myriad ways in which large AI models are homogenizing language and culture, averaging out rich linguistic differences into generic expressions. I call this phenomenon "softmaxing culture,'' and it is one of the fundamental challenges facing AI evaluations today. Efforts to improve and strengthen evaluations of culture are central to the project of cultural alignment in large AI systems. This position paper argues that machine learning (ML) and human-computer interaction (HCI) approaches to evaluation are limited. I propose two key conceptual shifts. First, instead of asking "what is culture?" at the start of system evaluations, I propose beginning with the question: "when is culture?" Second, while I acknowledge the philosophical claim that cultural universals exist, the challenge is not simply to describe them, but to situate them in relation to their particulars. Taken together, these conceptual shifts invite evaluation approaches that move beyond technical requirements toward perspectives that are more responsive to the complexities of culture.

James Luther, Donald Brown

Culture is the bedrock of human interaction; it dictates how we perceive and respond to everyday interactions. As the field of human-computer interaction grows via the rise of generative Large Language Models (LLMs), the cultural alignment of these models become an important field of study. This work, using the VSM13 International Survey and Hofstede's cultural dimensions, identifies the cultural alignment of popular LLMs (DeepSeek-V3, V3.1, GPT-5, GPT-4.1, GPT-4, Claude Opus 4, Llama 3.1, and Mistral Large). We then use cultural prompting, or using system prompts to shift the cultural alignment of a model to a desired country, to test the adaptability of these models to other cultures, namely China, France, India, Iran, Japan, and the United States. We find that the majority of the eight LLMs tested favor the United States when the culture is not specified, with varying results when prompted for other cultures. When using cultural prompting, seven of the eight models shifted closer to the expected culture. We find that models had trouble aligning with Japan and China, despite two of the models tested originating with the Chinese company DeepSeek.

Tomáš Evan, Eva Fišerová, Aneta Elgnerová

UN migration data and Hofstede's six cultural dimensions make it possible to find a connection between migration patterns and culture from a longterm perspective. Migrant patterns have been studied from the perspective of both immigrants and OECD host countries. This study tests two hypotheses: first, that the number of migrants leaving for OECD countries is influenced by cultural similarities to the host country; and second, that OECD host countries are more likely to accept culturally close migrants. Both hypotheses were tested using the Mann/Whitney U test for 93 countries between 1995 and 2015. The relationship between cultural and geodesic distance also analysed. The results indicate that cultural proximity significantly influences migration patterns, although the impact varies across countries. About two/thirds of OECD countries show a positive correlation between cultural similarity and geographic proximity, with notable exceptions, such as New Zealand and Australia, which exhibit a negative correlation. Countries such as Colombia, Denmark, and Japan maintain cultural distance, even from their neighbouring countries. Migrants from wealthier countries tend to select culturally similar destinations, whereas those from poorer countries often migrate to culturally distant destinations. Approximately half of OECD countries demonstrate a statistically significant bias towards accepting culturally close migrants. The results of this study highlight the importance of a critical debate that recognises and accepts the influence of culture on migration patterns.

Luyao Hao, Luyao Hao, Fengmiao Zhao et al.

In recent years, bovine viral diarrhea virus (BVDV) has been associated with increased respiratory and gastrointestinal diseases in cattle. Comprehensive monitoring and investigations into the virus's pathological features are crucial for developing effective prevention strategies. This study investigated BVDV prevalence and pathogenicity in farms undergoing elimination protocols, with a focus on characterizing a novel Cytopathic Bovine Viral Diarrhea Virus (CP-type BVDV) strain (HH839) isolated from a symptomatic calf in Hohhot, Inner Mongolia. During 2021 and 2022, 103 bovine samples were screened for BVDV via nucleic acid detection. Positive cases underwent viral isolation using MDBK cells. The HH839 strain was analyzed for cytopathic effects, ultrastructure (electron microscopy), antigenicity (serum neutralization), and genetic lineage (whole genome sequencing). Pathogenicity of Cytopathic Bovine Viral Diarrhea Virus (CP-type BVDV) infected group, Noncytopathic Bovine Viral Diarrhea Virus (NCP-type BVDV) infected group, and the mixed-infection group of CP-type and NCP-type BVDV was evaluated in New Zealand White rabbits, with viral distribution and histopathological damage assessed in multiple organs. We identified 33 positive BVDV nucleic acid cases, resulting in a positivity rate of 32.04%. Five strains of NCP-type BVDV were isolated, yielding a 15.15% separation rate, alongside one strain of CP-type BVDV with a separation rate of 3.03%. The CP strain HH839 was isolated from a severely symptomatic calf in Hohhot, Inner Mongolia. The HH839 strain demonstrated significant cytopathic effects in MDBK cells, including cellular crumpling and syncytia formation, with a concentration of 5.23 log10TCID50/0.1 mL. Electron microscopy revealed a spherical morphology with a diameter of 40–60 nm. Genetic analysis indicated a close relationship with the BVDV FBS-D8 strain from the BVDV-1d subtype. Pathogenicity trials showed slight fever and minor body weight loss in infected subjects, with BVDV detected in the trachea, lungs, spleen, and small intestines, predominantly in the spleen. The isolation of HH839, a pathogenic CP-type BVDV-1d strain, underscores the coexistence of multiple BVDV biotypes in regional cattle populations. Enhanced pathogenicity observed in mixed infections highlights complex viral interactions. These findings emphasize the necessity for sustained surveillance and biotype-specific control strategies to mitigate BVDV-associated economic losses in livestock industries.

Miguel Carrillo Parraguez, Miguel Carrillo Parraguez, Eduardo Ponssa et al.

Despite its sociocultural and economic importance, dairy farming in Uruguay has not experienced a significant increase in cattle stocks in recent decades, partly due to low reproductive efficiency and a high risk of calf mortality. Neospora caninum is one of the primary abortive pathogens in dairy cattle; however, the economic losses caused by neosporosis in Uruguay remain uncalculated. This study aimed to assess the direct annual economic losses for the primary dairy sector (dairy farmers) due to abortions resulting from bovine neosporosis in the Uruguayan herd. An adjusted bioeconomic model was used to estimate the economic losses from early (second gestational trimester) or late (third gestational trimester) abortions, considering the Present Value (PV) of future production loss due to these abortions after deducting production costs (lost profits). The average economic loss per abortion due to neosporosis was US$ 868 (range 605–1,162) if the aborting cow remained in the herd without any additional abortions throughout its productive life and US$ 1,866 (range 782–2,825) if the cow was culled after the abortion. Individual losses varied based on the gestational stage and the age of the aborting cow, with the worst-case scenario being a late abortion in first-pregnancy heifers. The annual number of abortions due to neosporosis was estimated by considering the stock of cows and heifers of reproductive age at risk of abortion (pregnant) and the national seroprevalence of N. caninum in dairy herds, accounting for 90% vertical transmission and 10% horizontal transmission, with aborting seropositive dams representing 15 and 35%, respectively. The economic losses for the national herd were calculated by multiplying individual losses by the number of abortions across various simulated scenarios (second or third gestational trimester), adjusted according to sub-scenarios (with and without culling of the aborted cow). The estimated economic losses for the primary sector due to abortions occurring in 2018 totaled nearly US$ 12 million, which is attributed to the loss of offspring, delayed lactation, reduced productive life (fewer days in milk production/days of life), and early culling. These estimated losses reflect only the primary sector, excluding the secondary (industrial) and tertiary (services) sectors. In physical terms, abortions associated with neosporosis caused a total loss of 62 million L of milk, which is 3.3% of the volume industrialized annually in the country. The estimated losses did not account for profits lost by dairy farmers from beef production (i.e., breeding, fattening, and sale for slaughter of male calves from dairy breeds lost due to neosporosis) nor any indirect losses (for example, the loss of genetic merit, stunted growth of the national dairy stock, etc.). The characteristics of Uruguay’s grazing dairy production system require a tailored approach to estimating the economic impact of bovine diseases. This information can be used by farmers, veterinarians, and policymakers to evaluate the cost–benefit of implementing control and prevention strategies for bovine neosporosis at both the farm and national levels.

Kim M. Tsoi, Qin Dai, B. Alman et al.