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DOAJ Open Access 2026
The Synergistic Impact of Polyphenols on Collagen Fiber–Starch Composite Films for Enhanced Physical Integrity and Antioxidant Capacity

Jiapeng Li, Jing Xu, Wenjian Cheng et al.

Edible films are increasingly recognized as promising sustainable packaging alternatives, but often face challenges such as poor mechanical strength, limited barrier properties, and low oxidative stability. This study aimed to enhance the physicochemical performance of collagen fiber–starch composite films by incorporating polyphenols (including tannic acid (TA), caffeic acid (CA), and their oxidized forms, OTA and OCA) as natural cross-linkers and antioxidants. Results showed that the addition of 0.1% TA increased the tensile strength by approximately 45% compared to the control, while simultaneously reducing the water vapor permeability from 1.32 to 1.26 g·mm/kPa·h·m<sup>2</sup>, with TA outperforming CA due to its higher molecular weight and stronger intermolecular interactions. Oxidized polyphenols further improved the mechanical and water vapor barrier properties via quinone-induced covalent cross-linking, thereby forming a denser film network. The films also exhibited enhanced UV–visible light shielding, with nearly complete ultraviolet blockage (transmittance is close to zero in the 200–280 nm range). Non-oxidized polyphenols showed higher antioxidant activity in the ABTS and reducing power assays, while release kinetics analysis revealed the highest release rate in 50% ethanol, indicating a pronounced solvent-dependent behavior. Specifically, films with 0.1% TA exhibited an ABTS radical scavenging activity of over 80%, significantly higher than the control. Overall, polyphenols effectively improve film performance through cross-linking and structural modification, offering a theoretical foundation for designing active packaging for targeted food systems.

Chemical technology
DOAJ Open Access 2025
Effect of river cleaning on lowland drainage in South‐Eastern Sumatra

Dwinata Aprialdi, Reza Mohammadpour, Afri Fajar et al.

Abstract We study a tropical river in South‐East Sumatra, where land drainage in the coastal zone has resulted in subsidence and increased flooding risks, exacerbated by sea tides. The novelty of this research is in determining the effect of tide on the lowland drainage system for forestry in a coastal tropical region and the impact of river cleaning on flood management. Five monitoring stations were set up along the Lebong Hitam river and its primary channels to observe flow characteristics, water level, and bathymetry. The results show how the tide effects water level in the river and the adjacent drainage area with Eucalyptus plantations. Cleaning of the river had a significant effect on each station and increased the discharge and velocity by more than five times and reduced the water depth by more than 40%. In light of this research, it can be concluded that the cleaning up process improves flood risk management by decreasing the water level and increasing the discharge and velocity at each of the upstream stations. The cleaning did not have a significant effect on downstream sections of the river where sea levels control the water level in the river to a large extent. The work provides an analysis of tidal river and provides recommendations for current and future drainage and water management.

River protective works. Regulation. Flood control, Disasters and engineering
DOAJ Open Access 2025
Climate change adaptation in agriculture food supply chains: a bibliometric and content analysis

Rahmanwali Sahar, Munjiati Munawaroh, Jawidullah Iqbal

Abstract This study explores the intellectual development and research landscape surrounding climate change adaptation in agricultural food supply chains through a combined bibliometric and content analysis. The primary objective is to map publication trends, identify key contributors, including countries, institutions, and journals, highlight the most influential documents, and uncover major thematic clusters and gaps in the existing literature. A hybrid methodology was employed, utilizing bibliometric tools such as Biblioshiny and VOSviewer to analyze 4113 peer-reviewed articles and conference papers retrieved from the Scopus database, covering the period from 2015 to 2025. The findings reveal a growing scholarly interest in climate change adaptation, with peak publication activity observed in 2023. The United States, China, and India emerged as the most active publishing countries, while institutions like China Agricultural University and Wageningen University were leading contributors. Core journals included Science of the Total Environment and Sustainability. The most frequently occurring keywords were climate change, agriculture, and adaptive management, with clusters focusing on climate-smart agriculture, water efficiency, greenhouse gas mitigation, and socio-economic resilience. The co-authorship network emphasized strong international collaborations, particularly among the USA, China, and Europe. A content analysis of the most cited documents stressed the effectiveness of conservation agriculture, nitrogen management, traditional farming practices, and remote sensing technologies as critical strategies for climate adaptation. Based on the results, practical recommendations are offered for business managers to adopt digital and resilient supply chain technologies, for policymakers to promote inclusive climate policies, for governments to invest in infrastructure and research, and for farmers to implement adaptive practices such as crop diversification and water-saving techniques. This study has several limitations that future studies will consider.

Environmental sciences
DOAJ Open Access 2024
Growth and pulpwood traits of Leucaena leucocephala and Eucalyptus camaldulensis at rainfed and irrigated sites in southern India

P.G. Suraj, R. Hegde, M. Varghese et al.

Growth and wood traits of Leucaena leucocephala subsp. glabrata were compared with India's most widely grown pulpwood, Eucalyptus. Three Leucaena seedlots and sixty-four Leucaena clones selected in plantations established from two Indian land races and a Hawaiian seed source were tested together with five commercial E. camaldulensis clones at two contrasting sites in southern India. At the wetter, irrigated site, the three sources of Leucaena clones had significantly greater 4-year height and similar mean stem diameter to the Eucalyptus clones, while the Leucaena seedlots were slower-growing. At the semi-arid, rainfed site, Eucalyptus grew faster and had better survival than the three sources of Leucaena clones, which again were similar in their performance and superior to the Leucaena seedlots. Basic densities, determined from breast-height wood cores, of 4-year-old Leucaena and Eucalyptus were quite similar (568 and 534 kg m-3 respectively) at the irrigated site, but at the rainfed site Eucalyptus wood density was higher (590 kg m-3), while that of Leucaena was lower (508 kg m-3). The kraft pulp yield (KPY) of Leucaena clones was about 1.5 % higher than Eucalyptus at both sites; KPYs of both species were 1.8 % lower at the rainfed than the irrigated site. Leucaena fibres were 31 % and 39 % longer and 65 % and 58 % wider than Eucalyptus, at the irrigated and rainfed sites respectively, and had lower cell wall proportions. Differences among Leucaena clones were significant for growth traits and for most wood and fibre traits, indicating the potential for selecting fast-growing Leucaena clones with improved wood properties. Clonal Leucaena plantations can serve as a productive and complementary pulpwood crop to Eucalyptus in southern India, increasing the yield and strength of blended pulps.

Forestry, Plant ecology
DOAJ Open Access 2024
Economic Evaluation of Conservation through Use of an <i>Araucaria angustifolia</i> Provenance and Progeny Test

José Arimatéia Rabelo Machado, Miguel Luiz Menezes Freitas, Daniela Ivana Paiva et al.

<i>Araucaria angustifolia</i> is a species known for its valuable wood and nuts, but it is threatened with extinction. The plantation of forests for genetic resource conservation is a complementary strategy designed to reduce the species’ genetic variability loss. This study aimed to evaluate the technical and economic viability of <i>A. angustifolia</i> for genetic conservation through use. The analyzed provenance and progeny trial was established in 1982 in Itapeva, Brazil. It was structured using a compact family blocks design with 110 open-pollinated progenies from five natural populations, three replicates, ten plants per subplot, and 3.0 m × 2.0 m spacing. After 33 years, the trial was evaluated for total height, diameter at breast height, wood volume, and survival. The variance components and genetic parameter estimates were performed using Restricted Maximum Likelihood/Best Linear Unbiased Prediction methods (REML/BLUP) methods with the Selegen software (version 2014). The production and management scenarios were obtained using the SisAraucaria software (version 2003). Sensitivity analysis and economic parameter estimates were obtained through various economic evaluation methods using the Planin software (version 1995). In general, the genetic parameters indicated that the population has enough variability for both conservation and breeding purposes, suggesting technical viability for the establishment of a seed orchard. The economic parameters indicated that the commercialization of wood and araucaria nuts proved to be more profitable than wood production by itself. In conclusion, araucaria genetic conservation through use is a technically and economically viable ex situ conservation strategy.

DOAJ Open Access 2023
Productivity, water and nitrogen utilization of intensified dryland farming with annual forages on the Chinese Loess plateau

Jianqiang Deng, Jianqiang Deng, Zhixin Zhang et al.

Understanding the relationships of productivity performance and water utilization and soil nitrogen dynamics after annual forage planting during the fallow period (F) in winter wheat (Triticum aestivum L.; W) mono-cropping is critically important for maintaining sustainable livestock and grain production in semiarid regions. We used 2 years (2017–2019) of data to investigate soil nitrogen dynamics, production, water utilization, and fallow efficiency when forage rape (Brassica campestris L.; R) and common vetch (Vicia sativa L.; V) were planted in a 3-month summer fallow of the W-F-W-F cropping system. Three cropping systems were comprised of winter wheat-summer fallow-winter wheat-summer fallow (W-F-W-F), winter wheat-forage rape-winter wheat-forage rape (W-R-W-R), and winter wheat-forage rape-winter wheat-common vetch (W-R-W-V). The results showed that the annual forage planting decreased the average NO3−-N content by 54.8% compared with the W-F-W-F cropping system. Compared with the W-F-W-F cropping system, planting annual forage in summer fallow increased the average system forage production by 4.93 t ha−1. Local total annual precipitation can meet crop-water requirements, and the limiting factor for agricultural production was the drought due to the uneven seasonal distribution of precipitation. In comparison to the W-F-W-F cropping system, annual forage planting decreased the average available soil moisture storage by 50.3 mm above the 80 cm soil layer. Compared with that in the W-R-W-R (23.21 t ha−1) and W-F-W-F (30.25 t ha−1) cropping systems, the crop productivity in the W-R-W-V cropping system (33.23 t ha−1) was relatively stable and high because the reduction in subsequent winter wheat yield (2.96 t ha−1) was adequately offset by the forage yield (5.15 t ha−1). Adding forage rape to the W-F-W-F cropping system decreased system crop-water productivity (CWP) by 40.9%. However, the CWP, precipitation use efficiency (PUE), and soil nitrate in the W-R-W-V cropping system increased by 30.4, 30.1, 110.9, and 82.0%, respectively, compared with those in the W-R-W-R cropping system. Therefore, the W-R-W-V cropping system is recommended for better water and fertility management as well as grain and forage production in semiarid regions. However, further study is required to involve drought years for better evaluation of the effect of long-term precipitation variability on the crop productivity.

Nutrition. Foods and food supply, Food processing and manufacture
DOAJ Open Access 2023
A comparative metabolomics analysis of domestic yak (Bos grunniens) milk with human breast milk

Wenhao Li, Weike Zeng, Weike Zeng et al.

Yaks are tough animals living in Tibet’s hypoxic stress environment. However, the metabolite composition of yak milk and its role in hypoxic stress tolerance remains largely unexplored. The similarities and differences between yak and human milk in hypoxic stress tolerance are also unclear. This study explored yak colostrum (YC) and yak mature milk (YMM) using GC–MS, and 354 metabolites were identified in yak milk. A comparative metabolomic analysis of yak and human milk metabolites showed that over 70% of metabolites were species-specific. Yak milk relies mainly on essential amino acids- arginine and essential branched-chain amino acids (BCAAs): L-isoleucine, L-leucine, and L-valine tolerate hypoxic stress. To slow hypoxic stress, human breast milk relies primarily on the neuroprotective effects of non-essential amino acids or derivates, such as citrulline, sarcosine, and creatine. In addition, metabolites related to hypoxic stress were significantly enriched in YC than in YMM. These results reveal the unique metabolite composition of yak and human milk and provide practical information for applying yak and human milk to hypoxic stress tolerance.

Veterinary medicine
DOAJ Open Access 2023
Two Sets of Boundary Conditions in Cyclical Systems with Goodwill in Capitalization

Petri P. Kärenlampi

Mathematical analysis is conducted on cyclical systems with goodwill in capitalization. Proportional goodwill vanishes with vanishing tangible value. Correspondingly, periodic boundary condition does not enable commercial utilization of the goodwill. Abandoning the periodic boundary condition enables commercial utilization of the goodwill. Even if a physical system is periodic, an agent can abandon the corresponding boundary condition by divesting. Example cases are shown in terms of boreal forestry systems.

DOAJ Open Access 2023
Optimization of the Ginkgo Wine Fermentation Process and Influence of Fermentation Starter Types on the Brewing Flavor

Bolin Chen, Fang Zhang, Zhifan Li et al.

<i>Ginkgo biloba</i> seed (GBS) contains rich nutrients, such as starch, protein, oil, and trace components, such as flavonoids and terpene lactones. Due to its high protein content, it can be used as a raw material for fermentation and brewing. In this study, GBS was selected as the object of a fermentation process optimization test. Six kinds of fermentation starter were selected to brew ginkgo wine. The results showed that different fermentation starters have significant impacts on the composition of the wine. The yeast group had higher total sugar content and comprehensive evaluation scores than the Jiuqu group, while the total acid and total free amino acid contents showed the opposite result. The total flavonoid and total terpene lactone contents of the yeast group were 21.0% and 12.8% higher than those of the Jiuqu group, respectively. However, the 4′-O-methylpyridoxine (MPN) and 4′-O-methylpyridoxine-5′-glucoside (MPNG) contents of the yeast group were also 12.6% and 2.3% higher than those of the Jiuqu group, respectively. The common volatile components in the two groups of samples were isoamyl alcohol, phenethyl alcohol, ethyl octanoate, and phenethyl acetate. The antioxidant capacity of ginkgo wine fermented by yeast was significantly higher than that of the Jiuqu group sample.

Fermentation industries. Beverages. Alcohol
DOAJ Open Access 2022
Antibiotic sensitivity of the causative microorganisms of subclinical mastitis in lactating sheep

Aleksandar Stoimenov, Teodora Popova, Georgi Stoimenov et al.

The aim of the presented research is to determine the antibiotic sensitivity and resistance of the microorganisms causing subclinical mastitis in dairy sheep. To achieve this, 120 milk samples from 4 farms located in 3 regions of the country were obtained and examined. The results showed that the isolates from the different farms were sensitive to Ciprofloxacin and Enrofloxacin, as well as to the combination Sulfamethoxazole+Trimethoprim. Resistance was found most often to Kanamycin, Colistin and antibiotics from the penicillin group.

Animal culture
DOAJ Open Access 2022
Abundance and accessibility of forage for reindeer in forests of Northern Sweden: Impacts of landscape and winter climate regime

Ilona Kater, Robert Baxter

Abstract The survival of reindeer during winter, their period of greatest food stress, depends largely on the abundance and accessibility of forage in their pastures. In Northern Sweden, realized availability of forage is notably affected by snow conditions and the impacts of forestry. While these factors have been examined in isolation, their combined effect has, to the best of our knowledge to date, not been researched. In this study, vegetation surveys and analysis of snow conditions were undertaken in forest stands at various stages of recovery from clear‐cutting. The variation in abundance and growth of understory species edible by reindeer, such as lichen, was noted as forests matured. The barrier effect of ice lenses in the snow was also measured in these stands. Lichen biomass was significantly affected by a combination of stand maturity, understory vegetation height, and lichen height. Soil disturbance from the processes of felling and competition in the vegetation communities recovering from this disturbance were identified as key drivers of change in lichen biomass. Overall, clear‐cut forests had some of the greatest prevalence of ice lenses in the snow column, and forage availability at these sites was up to 61% less than in mature stands over 58 years in age. It is suggested that alternative silviculture methods are investigated for use in this reindeer herding region, as frequent clear‐cutting and consequent reduction in the average forest stand age and maturity class may be detrimental to reindeer grazing, reducing both abundance of forage, and access to it during winter.

DOAJ Open Access 2022
Comparative Evaluation of a Newly Developed Trunk-Based Tree Detection/Localization Strategy on Leaf-Off LiDAR Point Clouds with Varying Characteristics

Tian Zhou, Renato César dos Santos, Jidong Liu et al.

LiDAR data acquired by various platforms provide unprecedented data for forest inventory and management. Among its applications, individual tree detection and segmentation are critical and prerequisite steps for deriving forest structural metrics, especially at the stand level. Although there are various tree detection and localization approaches, a comparative analysis of their performance on LiDAR data with different characteristics remains to be explored. In this study, a new trunk-based tree detection and localization approach (namely, height-difference-based) is proposed and compared to two state-of-the-art strategies—DBSCAN-based and height/density-based approaches. Leaf-off LiDAR data from two unmanned aerial vehicles (UAVs) and Geiger mode system with different point densities, geometric accuracies, and environmental complexities were used to evaluate the performance of these approaches in a forest plantation. The results from the UAV datasets suggest that DBSCAN-based and height/density-based approaches perform well in tree detection (F1 score > 0.99) and localization (with an accuracy of 0.1 m for point clouds with high geometric accuracy) after fine-tuning the model thresholds; however, the processing time of the latter is much shorter. Even though our new height-difference-based approach introduces more false positives, it obtains a high tree detection rate from UAV datasets without fine-tuning model thresholds. However, due to the limitations of the algorithm, the tree localization accuracy is worse than that of the other two approaches. On the other hand, the results from the Geiger mode dataset with low point density show that the performance of all approaches dramatically deteriorates. Among them, the proposed height-difference-based approach results in the greatest number of true positives and highest F1 score, making it the most suitable approach for low-density point clouds without the need for parameter/threshold fine-tuning.

DOAJ Open Access 2020
A physiological approach for pre-selection of Eucalyptus clones resistant to drought

Müller C, Hodecker BER, De Barros NF et al.

Water deficit is one of the abiotic stresses that most affects the growth and survival of Eucalyptus. Mechanisms used to tolerate water-limited environments influence the distribution of Eucalyptus species in their natural environment. Here, we take a physiological approach to pre-screen Eucalyptus plants for tolerance to drought. Ten different clones of E. urophylla and E. grandis × E. urophylla that are known to show contrasting responses to water deficit under field conditions, were grown in Clark’s nutrient solution (WW, well-watered) and with polyethylene glycol (-1.0 MPa) to simulate water deficit (WD). Clones responded differently to drought with differentiated photosynthetic limitations in drought-treated clones. Photosynthetic rates, stomatal conductance, transpiration and internal CO2 concentrations were reduced in all genotypes under stress conditions. Clone i144 had a smaller reduction in the evaluated physiological traits, also showing increased root growth in WD-treated plants. Clones 3367 and i224, thought to be moderately tolerant, also followed these patterns. Clones gg157, 1568 and 1641, all of which are moderately sensitive under field conditions, reduced most of the physiological characters evaluated. However, clone gg157 demonstrated increased root system growth, even during short periods of water stress. Clones i042 and i182 were deemed drought-susceptible, with large reductions in photosynthesis and growth, despite showing a high increase in abscisic acid content presumably as a defense mechanism. Interaction between A (photosyntetic rate), E (transpiration rate), ETR/A (electrons transport rate/photosynthetic rate) and SDM/ RDM (shoot dry matter/root dry matter) demonstrated the most significant differences between WD-treated clones and offer great potential for use as selection criterion for water deficit-tolerant genotypes.

DOAJ Open Access 2020
Diversity and Structure of Natural Regeneration in Swamp Forests in Southeastern Brazil

Marly Antonielle Ávila, Nayara Mesquita Mota, Saimo Rebleth Souza et al.

Abstract Many factors can influence the structures and distributions of plant communities. Plant diversity of swamp forests reflect responses to water stress conditions. We evaluated the floristic diversity, structure, and composition of the regenerating shrub-tree species of six swamp forests in veredas ecosystems (northern Minas Gerais, Brazil), and the floristic similarities between them. All individuals ≥ 1 cm in diameter at ground level and < 3 cm in diameter at breast height were sampled in one hundred 25 m2 plots in each area. A total of 5442 individuals were recorded, distributed among 134 species, 85 genera, and 47 botanical families, with a diversity (Shannon) of 3.38 and equability (Pielou) of 0.69. Beta diversity was high, while similarities between the areas were low, with only two species common to all six sites. The forests showed different compositions, diversities, and natural regeneration structures, reflecting their ecotone nature and past anthropic impacts.

DOAJ Open Access 2019
Sustainability Assessment of Alternative Strip Clear Cutting Operations for Wood Chip Production in Renaturalization Management of Pine Stands

Janine Schweier, Boško Blagojević, Rachele Venanzi et al.

In Mediterranean regions, afforested areas were planted to ensure the permanence of land cover, and to protect against erosion and to initiate the vegetation processes. For those purposes, pine species were mainly used; however, many of these stands, without silvicultural treatments for over fifty-sixty years, were in a poor state from physical and biological perspective, and therefore, clear-cutting on strips was conducted as silvicultural operation with the aim to eliminate 50% of the pine trees and to favor the affirmation of indigenous broadleaves seedlings. At the same time, the high and increasing demand of the forest based sector for wood biomass related to energy production, needs to be supplied. In a modern and multifunctional forestry, in which society is asking for sustainable forestry and naturalistic forest management, forestry operations should ideally be carried out in a sustainable manner, thus support the concept of sustainable forest management. All these aspects are also related to the innovation in forestry sector for an effective energetic sustainability. Three different forest wood chains were applied in pine plantations, all differing in the extraction system (animal, forestry-fitted farm tractor with winch, and double drum cable yarder). The method of the sustainability impact assessment was used in order to assess potential impacts of these alternative management options, and a set of 12 indicators covering economic, environmental, and social dimensions was analyzed. Further, to support decision makers in taking informed decisions, multi-criteria decision analysis was conducted. Decision makers gave weight towards the indicators natural tree regeneration and soil biological quality to support the achievement of the forest management goal. Results showed that first ranked alternative was case 2, in which extraction was conducted by a tractor with a winch. The main reason for that lies in the fact that this alternative had best performance for 80% of the analyzed criteria.

DOAJ Open Access 2018
Arbuscular Mycorrhizal Symbiosis Modulates Antioxidant Response and Ion Distribution in Salt-Stressed Elaeagnus angustifolia Seedlings

Wei Chang, Wei Chang, Xin Sui et al.

Elaeagnus angustifolia L. is a drought-resistant species. Arbuscular mycorrhizal symbiosis is considered to be a bio-ameliorator of saline soils that can improve salinity tolerance in plants. The present study investigated the effects of inoculation with the arbuscular mycorrhizal fungus Rhizophagus irregularis on the biomass, antioxidant enzyme activities, and root, stem, and leaf ion accumulation of E. angustifolia seedlings grown during salt stress conditions. Salt-stressed mycorrhizal seedlings produced greater root, stem, and leaf biomass than the uninoculated stressed seedlings. In addition, the seedlings colonized by R. irregularis showed notably higher activities of superoxide dismutase (SOD), catalase (CAT), and ascorbate peroxidase (APX) in the leaves of the mycorrhizal seedlings in response to salinity compared to those of the non-mycorrhizal seedlings. Mycorrhizal seedlings not only significantly increased their ability to acquire K+, Ca2+, and Mg2+, but also maintained higher K+:Na+ ratios in the leaves and lower Ca2+:Mg2+ ratios than non-mycorrhizal seedlings during salt stress. These results suggest that the salt tolerance of E. angustifolia seedlings could be enhanced by R. irregularis. The arbuscular mycorrhizal symbiosis could be a promising method to restore and utilize salt-alkaline land in northern China.

DOAJ Open Access 2017
Changes in the nutrition and health status of young Norway spruce stands in the Krkonoše Mts. in a 17-year period

Radek NOVOTNÝ, Bohumír LOMSKÝ, Vít ŠRÁMEK

For 17 Norway spruce stands located in the Krkonoše Mts. in the Czech Republic a long-term assessment of defoliation, height increment, foliage concentration of nutrients (N, P, Ca, K, Mg) and stress elements (S, F) was carried out. The results show a decrease of defoliation and a slight increase of height increment, which occur in accordance with the decreasing concentration of sulphur in spruce needles. However, neither sulphur concentration nor height increment trends are significant, suggesting that both the main pollution abatement and the growth resumption took place already in the 1990's. During the late 1990's the average spruce defoliation was greater than 35%, while since 2007 it has fluctuated between 18 and 25%, which corresponds with the figures for the forest stands located in the other regions of the Czech Republic. The evaluation of single nutrient concentrations detected occasional deficiencies of P and Mg at individual plots. The good N nutrition (&gt; 15 mg N.g-1) in combination with a significantly decreasing trend of P, K and Ca concentrations in Norway spruce needles may potentially constitute a problem in regard to both the future health and the stability of forest stands in the studied region.

DOAJ Open Access 2015
Ink Penetration of Uncoated Inkjet Paper and Impact on Printing Quality

Ren'ai Li, Yan Zhang, Yunfeng Cao et al.

This study investigated ink penetration through imaging technology, first by gray and contour mapping and then calculating the ink penetration depth by programing. Next, a series of further analyses were carried out, including average ink permeability, ink distributions, and printability of different uncoated inkjet paper with different parameters. The impact on ink penetration of the microstructure and hydrophilicity of the uncoated paper was also studied. The experimental results indicated that paper specimens with sizing agent were resistant to the ink, resulting in a slow and shallow ink penetration. Paper containing filler had a more hydrophilic surface and porous structure, leading to a faster and deeper ink penetration. However, the calendering operation could make the paper structure more compact and reduce the porosity and penetration depth. When an appropriate combination of sizing agent, filler content, and the calendering process was utilized, a more stable hue could be produced with improvements in optical density, saturation, and color.

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