Hasil untuk "Veterinary medicine"

H. Ueno, Takashi Mori, T. Fujinaga

James R. Rest, D. Narvaez

L. Guardabassi, S. Schwarz, D. Lloyd

P. Butaye, L. Devriese, F. Haesebrouck

Ferdinando F Andrade, D. Silva, A. Rodrigues et al.

Colistin has been extensively used since the middle of the last century in animals, particularly in swine, for the control of enteric infections. Colistin is presently considered the last line of defense against human infections caused by multidrug-resistant Gram-negative organisms such as carbapenemase-producer Enterobacterales, Acinetobacter baumanni, and Pseudomonas aeruginosa. Transferable bacterial resistance like mcr-genes was reported in isolates from both humans and animals. Researchers actively seek strategies to reduce colistin resistance. The definition of guidelines for colistin therapy in veterinary and human medicine is thus crucial. The ban of colistin use in swine as a growth promoter and for prophylactic purposes, and the implementation of sustainable measures in farm animals for the prevention of infections, would help to avoid resistance and should be encouraged. Colistin resistance in the human–animal–environment interface stresses the relevance of the One Health approach to achieve its effective control. Such measures should be addressed in a cooperative way, with efforts from multiple disciplines and with consensus among doctors, veterinary surgeons, and environment professionals. A revision of the mechanism of colistin action, resistance, animal and human use, as well as colistin susceptibility evaluation is debated here.

C. Atkins, J. Bonagura, S. Ettinger et al.

Michal Ješeta, Jana Antalíková, Adéla Doubravská et al.

The separation of human spermatozoa is an important step in therapy of human infertility. Given that male fertility is decreasing and, as a result, fertilization techniques based on microinjection of sperm into the cytoplasm are being used more intensively, this question is becoming increasingly relevant. In recent years, microfluidic sperm processing techniques have been increasingly used. These methods are simple and easy to use, however, the question is to what extent they select the correct sperm. They are essentially based mainly on motility and do not reflect other navigational approaches such as chemotaxis, thermotaxis or rheotaxis. This review compares traditional, advances and novel in vitro methods of sperm separation which are commonly used during human infertility therapy in context of in vivo sperm separation in female reproductive system.

Mengting Tang, Denghua Yin, Jianglong Que et al.

The Yangtze finless porpoise (<i>Neophocaena asiaeorientalis asiaeorientalis</i>, YFP) is the only freshwater cetacean species currently found in China’s Yangtze River. To accurately evaluate its genetic diversity and provide reliable molecular markers for population genetic studies, this study developed a highly efficient and reproducible method for identifying polymorphic microsatellite loci using whole-genome sequencing data. Using this method, we identified and validated a set of highly polymorphic microsatellite markers, which were then used to analyze the genetic diversity of the YFP populations in Poyang Lake to evaluate their effectiveness. Our results demonstrated that the screening pipeline successfully identified 220 tetranucleotide repeat microsatellite loci. Based on the principle of uniform chromosomal distribution, 190 loci were randomly selected for experimental validation, of which 19 exhibited stable amplification, high polymorphism, and a low genotyping error rate. Genetic diversity analysis based on these markers revealed significant genetic variation among YFP populations in Poyang Lake, confirming the effectiveness of the developed markers. The polymorphic microsatellite molecular marker system developed in this study demonstrates high reliability and applicability for assessing YFP genetic diversity. This system provides a critical technical foundation for future research in conservation genetics, genetic resource preservation, and the development of genetic management strategies for the species.

Ratna Dewi, Tongku Nizwan Siregar, Amalia Sutriana et al.

Background: Morinda citrifolia L. possesses antioxidant activity that can ameliorate the decline in semen quality of male rats due to exposure to cigarette smoke. Objectives: This study intend to assess the effectiveness of M._citrifolia leaves extract in ameliorating male infertility associated with oxidative dysregulation induced by exposure to tobacco smoke. Methods: The animals used in the study were evenly and randomly divided into five groups, each containing five rats. Group X1 served as the normal control without any treatment, whereas group X2 comprised rats that were exclusively subjected to cigarette smoke exposure. Groups X3, X4, and X5 were exposed to cigarette smoke and subsequently administered M. citrifolia leaves extract orally via a nasogastric tube at doses of 100, 200, and 300 mg/kg BW, respectively, for a period of 52 days. Twenty-four hours after the final treatment, blood samples were collected to examine FSH, LH, and testosterone levels using ELISA technique. Semen was collected from the cauda epididymis to analyze the quality of spermatozoa. Results: The administration of M. citrifolia leaves extract improved sperm concentration, progressive motility, and viability, while sperm morphological abnormalities were not affected by the extract (P = 0.618). FSH concentration decreased following M. citrifolia leaves extract administration, particularly at dose of 100, and 200 mg/kg BW. LH concentration increased significantly after treatment with 100_mg/kg BWof M. citrifolia leaves extract and testosterone levels improved after treated with leaves extract of M. citrifolia (P <0.001). Conclusions: Methanol extract of M. citrifolia leaves enhance sperm quality and testosterone levels but does not affect FSH and LH concentrations in male rats exposed to cigarette smoke.

Vefa Tohumcu, Mehmet Cengiz, A. Hayirli et al.

ABSTRACT Background Isoproterenol (ISO) is a nonselective beta‐adrenergic receptor agonist known for its vasodilatory effects. This experiment aims to investigate whether intrauterine ISO administration could alter vascular indices and follicular development in postpartum Holstein cows. Objectives The objectives are to evaluate the effects of intrauterine ISO administration on vascular changes and its impact on follicular development compared to placebo groups. Study Design This randomized controlled study was conducted on 36 Holstein cows selected based on their health status, including only those free from reproductive, metabolic and infectious disorders. Methods The cows (n = 36) were divided into two groups as control received distilled water alone (CON, n = 18) and experiment received 4 mg ISO in 40 mL distilled water (ISO, n = 18) and four subgroups as CON‐I (n = 9), CON‐II (n = 9), ISO‐I (n = 9) and ISO‐II (n = 9) according to days of intrauterine administration (I or II represents to 1 or 2 days after ovulation, respectively). Uterine and ovarian artery blood flows were assessed before and after administration by Doppler ultrasonography. Blood samples were collected both before and after administration (on Day 1 or 2) and on Days 3, 6 and 9 post‐ovulation for hormonal analysis. Antral follicle count (AFC) was recorded on the blood sampling days. Data were analysed via mixed model ANOVA. Results Intrauterine ISO administration significantly increased the pulse rate (PR) in the ovaries (89.4 vs. 65.5 bpm, p < 0.0001) and uterus (90.6 vs. 64.2 bpm, p < 0.0001). Early AFC (1–2.9 mm) decreased, whereas small AFC (3–4.9 mm) increased in the ISO groups. The weighted average antral follicle size (WAAFS) significantly increased in the ISO group but remained unchanged in the controls. Hormonal analysis revealed elevated levels of FSH (626 vs. 468 mIU/mL), AMH (61.3 vs. 46.4 ng/L), E2 (138 vs. 122 ng/L), P4 (15.3 vs. 10.6 ng/mL), IGF‐1 (62.6 vs. 25.1 ng/mL) and IGFBP‐3 (28.4 vs. 16.5 ng/mL) in the ISO groups (p < 0.0001). Conclusions The findings indicate that intrauterine administration of ISO on Day 1 post‐ovulation could be a promising ‘adjunct technique’ for future research focussed on minimizing dependence on exogenous hormones or improving the sensitivity of follicles to endogenous hormonal signals, thereby potentially enhancing oocyte yield.

Yameng Qiao, Wenzheng Liu, Fanzhen Wang et al.

Individual recognition of Hu sheep is a core requirement for precision livestock management, significantly improving breeding efficiency and fine management. However, traditional machine vision methods face challenges such as high annotation time costs, the inability to quickly annotate new sheep, and the need for manual intervention and retraining. To address these issues, this study proposes a solution that integrates automatic annotation and transfer learning, developing a sheep face recognition algorithm that adapts to complex farming environments and can quickly learn the characteristics of new Hu sheep individuals. First, through multi-view video collection and data augmentation, a dataset consisting of 82 Hu sheep and a total of 6055 images was created. Additionally, a sheep face detection and automatic annotation algorithm was designed, reducing the annotation time per image to 0.014 min compared to traditional manual annotation. Next, the YOLOv10n-CF-Lite model is proposed, which improved the recognition precision of Hu sheep faces to 92.3%, and the mAP@0.5 to 96.2%. To enhance the model’s adaptability and generalization ability for new sheep, transfer learning was applied to transfer the YOLOv10n-CF-Lite model trained on the source domain (82 Hu sheep) to the target domain (10 new Hu sheep). The recognition precision in the target domain increased from 91.2% to 94.9%, and the mAP@0.5 improved from 96.3% to 97%. Additionally, the model’s convergence speed was improved, reducing the number of training epochs required for fitting from 43 to 14. In summary, the Hu sheep face recognition algorithm proposed in this study improves annotation efficiency, recognition precision, and convergence speed through automatic annotation and transfer learning. It can quickly adapt to the characteristics of new sheep individuals, providing an efficient and reliable technical solution for the intelligent management of livestock.

Shilva Givanny Saiful, Mohandas Indradji, Diana Indrasanti et al.

Background: Infectious coryza, caused by Avibacterium paragallinarum, is an acute and highly contagious respiratory disease in chickens that results in high morbidity, growth retardation, and decreased egg production, leading to economic losses in poultry industries. Vaccination is considered the most effective preventive measure, and inactivated vaccines are widely used due to their safety and ability to stimulate protective immunity. Purpose: This study aimed to evaluate the safety and potency of a commercially produced inactivated coryza vaccine using Specific Pathogen-Free (SPF) chickens at the National Quality Testing and Certification Center for Veterinary Drugs (BPMSPH), Indonesia. Method: A descriptive observational approach was used to assess safety and potency in vaccinated SPF chickens. A total of 40 SPF chickens four weeks old were used and divided into a vaccinated group and a control group. The vaccinated group received the inactivated coryza vaccine according to standard test procedures, while the control group remained unvaccinated. Clinical observations and local reaction assessments were conducted to evaluate safety, and antibody titers against coryza serotype A were measured to determine potency. Results: Observations showed that 100% of both control and vaccinated chickens exhibited no abnormal clinical signs or coryza reactions. In addition, the vaccine potency test showed that 100% of vaccinated chickens had coryza serotype A antibody titers ≥10. Conclusion: This test confirms that the registered inactivated coryza vaccine formulation meets safety test criteria: 100% of control and vaccinated chickens remained clinically normal, and no abnormal local reactions were observed at the inoculation site. Potency testing demonstrated that all vaccinated chickens developed serotype A antibodies, which are expected to provide protection against coryza infection in the field and improve poultry survival.

Łukasz Grabowski, Lidia Gaffke, Karolina Pierzynowska et al.

Enrofloxacin is a compound that originates from a group of fluoroquinolones that is widely used in veterinary medicine as an antibacterial agent (this antibiotic is not approved for use as a drug in humans). It reveals strong antibiotic activity against both Gram-positive and Gram-negative bacteria, mainly due to the inhibition of bacterial gyrase and topoisomerase IV enzymatic actions. The high efficacy of this molecule has been demonstrated in the treatment of various animals on farms and other locations. However, the use of enrofloxacin causes severe adverse effects, including skeletal, reproductive, immune, and digestive disorders. In this review article, we present in detail and discuss the advantageous and disadvantageous properties of enrofloxacin, showing the benefits and risks of the use of this compound in veterinary medicine. Animal health and the environmental effects of this stable antibiotic (with half-life as long as 3–9 years in various natural environments) are analyzed, as are the interesting properties of this molecule that are expressed when present in complexes with metals. Recommendations for further research on enrofloxacin are also proposed.

M. Steffey, D. Griffon, M. Risselada et al.

Chronic workplace stress and burnout are serious problems in veterinary medicine. Although not classified as a medical condition, burnout can affect sleep patterns and contributes to chronic low grade systemic inflammation, autonomic imbalance, hormonal imbalances and immunodeficiencies, thereby increasing the risks of physical and psychological ill health in affected individuals. Cultural misconceptions in the profession often lead to perceptions of burnout as a personal failure, ideas that healthcare professionals are somehow at lower risk for suffering, and beliefs that affected individuals can or should somehow heal themselves. However, these concepts are antiquated, harmful and incorrect, preventing the design of appropriate solutions for this serious and growing challenge to the veterinary profession. Veterinarians must first correctly identify the nature of the problem and understand its causes and impacts before rational solutions can be implemented. In this first part of two companion reviews, burnout will be defined, pathophysiology discussed, and healthcare and veterinary-relevant occupational stressors that lead to burnout identified.

Rachel Malkani, Sharmini Paramasivam, Sarah Wolfensohn

Behavioural disorders in dogs are common and have severe welfare consequences for dogs. This study aimed to assess the factors that are significant and predictive of behaviour problems in dogs using the animal welfare assessment grid (AWAG) to further understand what factors influence their welfare. 177 AWAG assessments were undertaken across 129 dogs that clinicians deemed to have a behavioural disorder. Wilcoxon rank-sum tests were used to assess the difference in scores between dogs with behaviour disorders and a cohort of healthy dogs (<i>n</i> = 117). This analysis showed that all physical factors besides body condition, all procedural factors besides procedure pain, and all psychological, and environmental factors were significantly different between healthy dogs and dogs with behaviour disorders. Spearman rank correlation coefficient (RS) revealed several significant strong positive correlations including the procedural impact on the dog’s daily routine with aggression towards unfamiliar people and procedure pain, as well as other correlations between the dog’s behaviour during assessment with the frequency at which they encounter fears and anxieties, clinical assessment and procedure pain, and reaction to stressors and social interactions. These findings highlight the interdependent nature of the various influences of welfare. Logistic regression analysis identified that aggression towards the caregiver, fears and anxieties frequency, and choice, control, and predictability were all significant predictors of behaviour disorders. The findings have important implications for veterinary, behaviour, and animal welfare professionals as any changes across these factors may indicate poor welfare linked to emotional disorders in dogs.

J. Harkness, J. E. Wagner

C. Tóthová, O. Nagy, G. Kováč

The acute phase response is a complex systemic early-defence system of reactions activated by trauma, infection, tissue damage, inflammation, stress or neoplasia. One of the most important elements of this response is the increased hepatic synthesis of some plasma proteins, collectively known as acute phase proteins. The dis - covery of these new biomarkers has allowed the clinical monitoring of different diseases; therefore, their clinical application has been studied widely in human medicine in order to improve the diagnosis, evaluation, treatment, prognosis and therapeutics of many diseases. Although a wide range of studies have been carried out to determine the usefulness of acute phase proteins in several diseases also in animals, they are still relatively under-utilised in veterinary medicine, predominantly in farm animals. The acute phase response and clinical application of acute phase proteins in ruminants are reviewed in this article, including their diagnostic use in clinical practice and application in the monitoring of treatment, which is one of the most promising practical uses of these proteins.

K. Hopkins, R. Davies, E. Threlfall

Sonal Gupta, Gaurav Tripathi, Kartikey Patel et al.

Buffalo cloning is a powerful assisted reproductive tool for multiplying elite buffalo germplasm. However, the live off-spring production efficiency is low due to aberrant epigenetic reprogramming. Aberrant epigenetic marks can be modified by culturing donor cells and/or one cell stage fused embryo or both with epigenetic modifiers alone or in combination. In the present study, we examined the effect of oxamflatin (OxF), ascorbic acid (AA), and their combined (OxF+AA) effect on in vitro developmental competence, quality, and pregnancy establishment rate of buffalo cloned embryos. Oxamflatin is a histone deacetylase inhibitor, whereas ascorbic acid is a hypomethylating agent. To achieve this aim of the study, reconstructs (fused two enucleated ooplasm + donor cell) were cultured for 8 h, i.e., 4 h post-fusion and 4 h post-activation with 1 μM oxamflatin (OxF), 50 μM ascorbic acid (AA), and there combined (OxF+AA) treatment. There was no significant (p<0.05) difference in cleavage rates when reconstructs were treated with oxamflatin (81.34±0.81%), ascorbic acid (82.76±0.51%), combined treatment (82.17±0.54%) compared with control (82.87±0.63%). The blastocyst production rate was significantly higher (p<0.05) in combined treatment OxF+AA (41.64±0.95%) as compared to OxF (34.88±1.22%), AA (38.99±0.69%) and control (30.29±0.77%). The TUNEL assay showed a significantly lower (p<0.05) apoptotic index in combined (OxF+AA) treatment (1.43±0.43) as compared to oxamflatin (3.54±0.46), ascorbic acid (3.24±0.49) and control (5.06±0.48). The cloned embryos were transferred to the synchronized recipient (n=15 to18 buffaloes in each group), and the conception rate was observed better in combined treatment (OxF+AA) (46.66%) than oxamflatin (16.66%) and ascorbic acid (12.50%). At the same time, no pregnancy was reported in the control group. In conclusion, the combined treatment with oxamflatin and ascorbic acid improves the in vitro and in vivo developmental potential in buffalo-cloned embryos, which could probably be due to decreased methylation and increased acetylation of the embryos.

Letitia Stana, Alexandra Trif, Loredana Gabriela Stana et al.

The aim of the experiment was to highlight the toxicity of the CrVI, administered during three generations, on the haemoglobin and on the osmotic resistance of the erythrocyte membrane in female rats. The determinations have been carried out on rat blood collected from female rats that reached sexual maturity, from F0, F1, F2 generation, exposed to LOAEL, of CrVI, as potassium dichromate, in drinking water (3 months). F1 and F2 were obtained from female rats from generation F0 and respectively F1 exposed for 3 months to chromium dose, mated with male rats exposed to the same dose for three months, before mating. The results of the experiment indicated a high significant decrease of haemoglobin, (p<0,01) under to the control lot and under physiologic limits, in the three generations. The decrease of hemoglobin, registered also high significant differences (p<0,01) as well between generation F0 and respectively generation F1 and F2. The chromium toxicity impact on erythrocyte membrane was shown by the progressive decrease of osmotic resistance even from the first generation and by the increase of the haemolysis degree in hypotonic solutions. The exposure to LOAEL CrVI during three generations produces toxic effects on erythrocytes inducing hemolytic effect.