Hasil untuk "Veterinary medicine"

M. Bilal, S. Mehmood, T. Rasheed et al.

Abstract Antibiotics are rightly regarded as one of the marvelous scientific discoveries of the 20th century that revolutionized both veterinary and human medicine. Nevertheless, the antibiotics have been realized as emerging environmental pollutants because of their massive administration in humans and animals and longer environmental persistence. Low concentrations of antibiotics are often detected in seawater, groundwater, surface water, and even drinking water. Parent antibiotic molecules or their metabolites are discharged into the aquatic environment giving rise to virtual or persistent ecological contamination, as well as the emergence of antibiotic resistance. Therefore, concerns have increased regarding source, occurrence, and consequences of the antibiotics or their active residues in aqueous environments. Their potential toxicity, teratogenicity, and genotoxicity on the aquatic organisms have grabbed noticeable attention as a serious environmental apprehension. This work covers up-to-date information on the occurrence, sources (surface water, underground water, and seawater), and consequences of the different antibiotics or their active residues on human health, microbiome, and various aqueous environment systems.

J. Ruiz

John Wright, N. Small, P. Raynor et al.

S. Schwarz, C. Kehrenberg, B. Doublet et al.

S. Brown, C. Atkins, Bagley Rs et al.

E. Kosciuczuk, P. Lisowski, J. Jarczak et al.

Cathelicidins are small, cationic, antimicrobial peptides found in humans and other species, including farm animals (cattle, horses, pigs, sheep, goats, chickens, rabbits and in some species of fish). These proteolytically activated peptides are part of the innate immune system of many vertebrates. These peptides show a broad spectrum of antimicrobial activity against bacteria, enveloped viruses and fungi. Apart from exerting direct antimicrobial effects, cathelicidins can also trigger specific defense responses in the host. Their roles in various pathophysiological conditions have been studied in mice and humans, but there are limited information about their expression sites and activities in livestock. The aim of the present review is to summarize current information about these antimicrobial peptides in farm animals, highlighting peptide expression sites, activities, and future applications for human and veterinary medicine.

M. T. Sweeney, B. Lubbers, S. Schwarz et al.

Md. Zahangir Hosain, S. Kabir, M. Kamal

Antimicrobial is an indispensable part of veterinary medicine used for the treatment and control of diseases as well as a growth promoter in livestock production. Frequent use of antimicrobials in veterinary practices may lead to the residue in animal originated products and creates some potential problems for human health. The presence of antimicrobial residues in animal originated foods may induce serious health problems such as allergic reaction, antimicrobial resistance (AMR), and lead to carcinogenic and mutagenic effects in the human body. The misuse or abuse of antibiotics in human medicine is thought to be a principal cause of AMR but some antimicrobial-resistant bacteria and their resistant genes originating from animals are also responsible for developing AMR. However, the residual effect of antimicrobials in feed and food products of animal origin is undeniable. In developing countries, the community is unaware of this residual effect due to lack of proper information about antibiotic usage, AMR surveillance, and residue monitoring system. It is imperative to reveal the current situation of antimicrobial use in livestock production and its impacts on public health. Moreover, the safety levels of animal feeds and food products of animal origin must be strictly monitored and public awareness should be developed against the indiscriminate use of antimicrobial in animal production. Therefore, the current review summarizes the literature on antimicrobial use in livestock production and its hazardous residual impacts on the human body in developing countries.

U. Binsker, A. Käsbohrer, J. Hammerl

Abstract The dramatic global rise of MDR and XDR Enterobacterales in human medicine forced clinicians to the reintroduction of colistin as last-resort drug. Meanwhile, colistin is used in the veterinary medicine since its discovery, leading to a steadily increasing prevalence of resistant isolates in the livestock and meat-based food sector. Consequently, transmission of resistant isolates from animals to humans, acquisition via food and exposure to colistin in the clinic are reasons for the increased prevalence of colistin-resistant Enterobacterales in humans in the last decades. Initially, resistance mechanisms were caused by mutations in chromosomal genes. However, since the discovery in 2015, the focus has shifted exclusively to mobile colistin resistances (mcr). This review will advance the understanding of chromosomal-mediated resistance mechanisms in Enterobacterales. We provide an overview about genes involved in colistin resistance and the current global situation of colistin-resistant Enterobacterales. A comparison of the global colistin use in veterinary and human medicine highlights the effort to reduce colistin sales in veterinary medicine under the One Health approach. In contrast, it uncovers the alarming rise in colistin consumption in human medicine due to the emergence of MDR Enterobacterales, which might be an important driver for the increasing emergence of chromosome-mediated colistin resistance.

Zhuo Lan, Xue Wang, Yuxi Zhang et al.

Clonorchiasis, caused by Clonorchis sinensis, is a significant public health issue in China and East Asia. However, understanding its pathological mechanisms underlying this disease remains limited. Here, we isolated and characterized C. sinensis extracellular vesicles (CsEVs) and evaluated their uptake by Kupffer cells (KCs) in vitro in immortalized KC (ImKC) line and in vivo in a model of C. sinensis infection, investigating genes with altered gene expression after treatment with CsEVs and during progression to liver fibrosis. CsEVs were successfully taken up by KCs to regulate gene expression. RNA-sequencing analysis identified 694 differentially expressed genes (DEGs), including upregulation of those encoding colony-stimulating factor 3 (CSF3), IL1b, and others. Further validation showed increased expressions of these genes in mice infected with C. sinensis. Gene ontology and Kyoto Encyclopedia of Genes and Genomes pathway analyses suggested that these DEGs were involved in pathways such as TNF signaling, and NF-κB signaling. During liver fibrosis progression, CSF3 expression positively correlated with alpha smooth muscle actin (α-SMA) levels in the liver, both of which were higher compared with negative controls. CSF3 inhibition caused a significant decrease in α-SMA expression. This study was the first to report differential gene expression changes in ImKCs stimulated by CsEVs, with CSF3, the most significantly upregulated gene, having a potential role in liver fibrosis. These findings provide important data for the pathology of clonorchiasis and could identify new targets for treatment.

Varish Ahmad, M. S. Khan, Q. Jamal et al.

Jane E Sykes, Thierry Francey, Simone Schuller et al.

Abstract Since publication of the last consensus statement on leptospirosis in dogs, there has been revision of leptospiral taxonomy and advancements in typing methods, widespread use of new diagnostic tests and vaccines, and improved understanding of the epidemiology and pathophysiology of the disease. Leptospirosis continues to be prevalent in dogs, including in small breed dogs from urban areas, puppies as young as 11 weeks of age, geriatric dogs, dogs in rural areas, and dogs that have been inadequately vaccinated for leptospirosis (including dogs vaccinated with 2‐serovar Leptospira vaccines in some regions). In 2021, the American College of Veterinary Internal Medicine (ACVIM) Board of Regents voted to approve the topic for a revised Consensus Statement. After identification of core panelists, a multidisciplinary group of 6 experts from the fields of veterinary medicine, human medicine, and public health was assembled to vote on the recommendations using the Delphi method. A draft was presented at the 2023 ACVIM Forum, and a written draft posted on the ACVIM website for comment by the membership before submission to the editors of the Journal of Veterinary Internal Medicine. This revised document provides guidance for veterinary practitioners on disease in dogs as well as cats. The level of agreement among the 12 voting members (including core panelists) is provided in association with each recommendation. A denominator lower than 12 reflects abstention of ≥1 panelists either because they considered the recommendation to be outside their scope of expertise or because there was a perceived conflict of interest.

Hiba S. Alnaemi, Tamara N. Dawood, Qais Th. Algwari

Objective: The efficiency of corona discharge (CD) for detoxification of aflatoxin B1 (AB1), ochra¬toxin A (OA), and fumonisin B1 (FMB1) from poultry feeds with its influences on feed components was investigated. Materials and Methods: Feed samples were exposed to CD for six durations (10, 20, 30, 40, 50, and 60 min) at three distances (1.5, 2.5, and 3.5 cm). Mycotoxin levels were estimated by compet¬itive enzyme-linked immunosorbent assay, and findings were substantiated by high-performance liquid chromatography. Results: AB1, OA, and FMB1 degradation percentages increased significantly (p < 0.05) with pro¬cessing times increment and distances reduction to reach values of 83.22%, 84.21%, and 84.76% at the first distance; 80.28%, 84.00%, and 84.12% at the second distance; and 68.30%, 71.74%, and 76.18% at the third distance, respectively, after 60 min of treatment. FMB1 reported the highest degradation level. Concerning CD impacts on feed composition, protein, fat, and moisture contents decreased significantly (p < 0.05). Carbohydrates and ash were not affected adversely. Depending on peroxide values estimation, fats were of good quality. Conclusion: The CD effectiveness for AB1, OA, and FMB1 detox from poultry feeds with moderate impact on the quality of feed. [J Adv Vet Anim Res 2024; 11(4.000): 819-834]

Seham A.H. Hassan, Zaki Z. Sharawy, Shabaan A. Hemeda et al.

The role of sugarcane bagasse (SCB) as a cheap carbohydrate (CHO) source was evaluated in L. vannamei larvae on the zootechnical indices and the countenance of growth, immune and antioxident genes in the liver and muscle tissues. The experiment includes two treatments, the control which feeds a commercial diet and the SCB treatment. It continued for 45 days in outside ponds in ternary (each supplied with six hundred shrimp larvae). The results revealed that both growth and survival rates of SCB treatment were significantly improved (P < 0.05) when versus to the basal. The hepatic (H) and muscular (M) genes expression increased considerably in the SCB treatment (P ≤ 0.05) versus control. The SCB treatment was the highest in all mRNA transcripts of studied immune-related genes. The expression of antioxidant genes superoxide destmutase (SOD) and glutathione peroxidase (GPx) in both H and M were increased significantly in SCB treatment relative to the control. Generally, the results highlight the importance of inclusion SCB in a zero water exchange system without an artificial diet ameliorates both zootechnical and immunity in L. vannamei.

K. Quesenberry, J. W. Carpenter

Maaike C. Swets, Steven Kerr, James Scott-Brown et al.

Abstract Choosing optimal outcome measures maximizes statistical power, accelerates discovery and improves reliability in early-phase trials. We devised and evaluated a modification to a pragmatic measure of oxygenation function, the $$S/F$$ S / F ratio. Because of the ceiling effect in oxyhaemoglobin saturation, $$S/F$$ S / F ratio ceases to reflect pulmonary oxygenation function at high $${S}_{p}{O}_{2}$$ S p O 2 values. We found that the correlation of $$S/F$$ S / F with the reference standard ( $${P}_{a}{O}_{2}$$ P a O 2 / $${F}_{I}{O}_{2}$$ F I O 2 ratio) improves substantially when excluding $${S}_{p}{O}_{2} > 0.94$$ S p O 2 > 0.94 and refer to this measure as $$S/{F}_{94}$$ S / F 94 . Using observational data from 39,765 hospitalised COVID-19 patients, we demonstrate that $$S/{F}_{94}$$ S / F 94 is predictive of mortality, and compare the sample sizes required for trials using four different outcome measures. We show that a significant difference in outcome could be detected with the smallest sample size using $$S/{F}_{94}$$ S / F 94 . We demonstrate that $$S/{F}_{94}$$ S / F 94 is an effective intermediate outcome measure in COVID-19. It is a non-invasive measurement, representative of disease severity and provides greater statistical power.

Hossein Navaei

Any disease or infection that can spread spontaneously from animals to humans or humans to animals is called zoonosis. The origin of more than 60% of human infections is zoonotic diseases. It covers many pathogens, including bacteria, viruses, fungi, protozoa, and parasites. The emergence, distribution, and patterns of zoonoses are significantly influenced by several factors, including climate change, animal movement, agentrelated factors, natural factors, and human impacts. Q fever has been neglected as a zoonotic disease in many developing countries. The causative agent of this disease is the bacterium Coxiella burnetii (C. burnetii), which is resistant to environmental factors such as heat and many disinfectant compounds, resulting in long-term risk of disease for humans and animals. Since the infection is usually asymptomatic, it is mainly undiagnosed in animals until adverse pregnancy outcomes occur in a herd. In humans, infection leads to severe endocarditis and vascular infection in chronic cases. Despite the importance of this disease, limited information is available about the molecular epidemiology and evolution of this pathogen. Genomic studies can also help to investigate the prevalence of this disease. Likewise, the pathogenesis of C. burnetii should be examined by molecular studies. Programs of awareness and ensuring the pasteurization of dairy products before human consumption will help prevent many zoonotic diseases, including Q fever.

Kate L. Hepworth‐Warren, Krista Estell, Bobby Cowles et al.

Abstract Background Serum amyloid A (SAA) is a major acute phase protein in horses which could be a useful tool for assessing clinical response to treatment of bacterial pneumonia in adult horses. Objectives To monitor SAA concentration in response to treatment and identify associations among SAA concentration, WBC and neutrophil counts, and fibrinogen in bacterial pneumonia in adult horses. Animals Eighteen adult horses with bacterial pneumonia. Methods Prospective clinical study. Horses hospitalized with bacterial pneumonia were enrolled and SAA concentration and vital signs were assessed daily. SAA concentration was measured by a handheld meter. CBC and plasma fibrinogen were assessed on days 0, 1, and 2, then every 3 days until discharge. Data were not normally distributed and therefore were log transformed. Log‐transformed data were analyzed and comparisons were performed on LSMeans by the 2‐sided Student's t‐test at the 5% level of significance. Results Geometric mean SAA concentration on day 0 was 537 μg/mL (SE 383 μg/mL). Geometric mean SAA concentration decreased significantly over time (P = .0001), peaking at day 2 (geomean 1038 μg/mL, SE 261.7 μg/mL) and decreasing until discharge. Plasma concentration of fibrinogen (P = .06), neutrophil count (P = .48), and WBC count (P = .07) did not change significantly over time. Conclusions and Clinical Importance SAA concentration decreased significantly over the course of treatment and correlated with clinical improvement of pneumonia whereas fibrinogen, neutrophil, and WBC counts did not.

Marwan Haddad, Doa Nassar, Munqez Shtaya

Abstract Impacts of nine heavy metals (Cd, Cr, Cu, Ni, Pb, K, Fe, Mn, and Zn) contamination in irrigation water on the soil, shoots, and roots of barely were investigated. Due to freshwater shortages, the use of available and inexpensive urban wastewater with input from local industrial factories containing heavy metals in irrigation is still practiced in the Middle East including Palestine. Barely was grown in plastic pots filled with sandy soil irrigated with simulated treated wastewater during two growing seasons. The metal treatments investigated include one, three, nine, and 15 multiples of the average metal content of treated effluent. Results showed that (i) Barley showed similar growth responses but different metal uptake patterns, (ii) Cd, Fe, Pb, and Zn in roots and shoots of barley were higher than WHO permissible levels, (iii) all metals accumulated in the soil had lower content than WHO permissible levels, (iv) The average value of enrichment factor (EF) for most heavy metals used was around unity indicating poor enrichment to soil and translocation to roots and shoots, (v) The highest Translocation factor (TF = 57.8) and Bioconcentration Factor (BCF = 126.8) was observed for K indicating its role in enhancing barley's tolerance to drought and its effectiveness in using barley in phytoremediation, and (vi) Barley growth and development and soil quality parameters were significantly affected by repetitive and increased irrigation with wastewater containing heavy metals.

Ionuţ Silviu BEIA, Roxana CICEOI, Violeta Elena BEIA et al.

Mycotoxin contamination in animal feed is a significant point of concern within the European food supply network. These toxic secondary metabolites produced by fungi have the potential to contaminate feed and pose a risk of making their way into the human diet via animal products like meat, milk, and eggs. The consequences of mycotoxin contamination extend beyond animal health, affecting economies and public health, presenting complex issues for agricultural producers, regulatory bodies, and consumers. Even with stringent controls and surveillance in place, mycotoxins remain a persistent issue. This study provides updated findings on the prevalence of mycotoxins globally and specifically in Europe, with a focus on analyzing the incidence of ochratoxin A both worldwide and within European contexts. The dispersion of mycotoxina within a given region can have considerable economic consequences for the trade of animal feed, directing stakeholders toward making informed decisions about the types of analyses in which they should allocate more resources.