The spectral characteristics of dextran, labeled with fluorescein, depend upon pH. We have loaded the lysosomes of mouse peritoneal macrophages with this fluorescence probe and used it to measure the intralysosomal pH under various conditions. The pH of the medium has no effect on the intralysosomal pH. Weakly basic substances in the medium cause a concentration-dependent increase in the intralysosomal pH. However, the concentration of base necessary to produce a significant change in the intralysosomal pH varies over a wide range for different bases. The active form of the base is the neutral, unprotonated form. Although most of these weak bases cause an increase in the volume of the lysosomes, increase in lysosomal volume itself causes only a minor perturbation of the intralysosomal pH. This was demonstrated in cells whose lysosomes were loaded with sucrose, and in cells vacuolated as a demonstrated in cells whose lysosomes were loaded with sucrose, and in cells vacuolated as a consequence of exposure to concanavalin A. The results of these studies are interpreted in terms of energy-dependent lysosomal acidification and leakage of protons out of the lysosomes in the form of protonated weak bases.
Space weather predictions of the solar wind impacting Earth are usually first based on remote-sensing observations of the solar disc and corona, and eventually validated and/or refined with in-situ measurements taken at the Sun$-$Earth Lagrange L1 point, where real-time monitoring probes are located. However, this pipeline provides, on average, only a few tens of minutes of lead time, which decreases to $\sim$30 minutes or less for large solar wind speeds of $\sim$800 km/s and above. The G5 geomagnetic storm of 2024 May provided an opportunity to test predictions generated employing real-time data from the STEREO-A spacecraft, placed 13° west of Earth and 0.04 au closer to the Sun than L1 at the time of the event, as shown recently by Weiler et al. (2025). In this Commentary, we contextualise these results to reflect upon the advantages of measuring the solar wind in situ upstream of L1, leading to improvements in both fundamental research of interplanetary physics and space weather predictions of the near-Earth environment.
During a solar flare, the fluxes in various lines and continua of the solar spectrum increase, leading to enhanced ionization of the illuminated part of the Earth's ionosphere and an increase in the total electron content (TEC). It has been previously shown that nearly 50% of X-class solar flares exhibit a second peak in warm coronal lines, such as FeXV and FeXVI (called the 'EUV late phase'), the effect of which on the ionosphere remains largely unexplored. This study presents an analysis of the ionospheric response to 14 X-class flares with pronounced late phases. For the first time, empirical relationships between the increase in TEC and the solar flux enhancement during the impulsive and late phases of the flare are derived. Additionally, we demonstrate the influence of flare location on the intensity of geoeffective solar spectral lines and the ratio of the ionospheric responses to the impulsive and late phases of solar flares.
The dynamics of the Earth's outer radiation belt, filled by energetic electron fluxes, is largely controlled by electron resonant interactions with electromagnetic whistler-mode waves. The most coherent and intense waves resonantly interact with electrons nonlinearly, and the observable effects of such nonlinear interactions cannot be described within the frame of classical quasi-linear models. This paper provides an overview of the current stage of the theory of nonlinear resonant interactions and discusses different possible approaches for incorporating these nonlinear interactions into global radiation belt simulations. We focused on observational properties of whistler-mode waves and theoretical aspects of electron nonlinear resonant interactions between such waves and energetic electrons.
Space plasma studies frequently use in situ magnetic field measurements taken from many spacecraft simultaneously. A useful data product of these measurements is the reconstructed magnetic field in a volume near the spacecraft observatory. We compare a standard method of computing the magnetic field at arbitrary spatial points, the Curlometer, to two novel approaches: a Radial Basis Function interpolation and a time-dependent 2D inverse distance weighted interpolation scheme called Timesync. These three methods, which only require in situ measurements of the magnetic fields and bulk plasma velocities at a sparse set of spatial points, are implemented on synthetic data drawn from a time-evolving numerical simulation of plasma turbulence. We compare both the topology of the reconstructed field to the ground truth of the simulation and the statistics of the fluctuations found in the reconstructed field to those from the simulated turbulence. We conclude that the Radial Basis Function and Timesync methods outperform the Curlometer in both the topological and statistical comparisons.
AGILE is one of the satellites currently detecting terrestrial gamma-ray flashes (TGFs). In particular, the AGILE Mini-CALorimeter detected more than 2000 events in 8 years activity, by exploiting a unique sub-millisecond timescale trigger logic and high-energy range. A change in the onboard configuration enhanced the trigger capabilities for the detection of these events, overcoming dead time issues and enlarging the detection rate of these events up to $>$50 TGFs/month, allowing to reveal shorter duration flashes. The quasi-equatorial low-inclination ( 2.5$^{\circ}$) orbit of AGILE allows for the detection of repeated TGFs coming from the same storms, at the same orbital passage and throughout successive orbital overpasses, over the same geographic region. All TGFs detected by AGILE are fulfilling a database that can be used for offline analysis and forthcoming studies. The limited number of missions currently detecting these brief terrestrial flashes makes the understanding of this phenomenon very challenging and, in this perspective, the AGILE satellite played and still plays a major role, helping shedding light to many aspects of TGF science
Collisionless shocks heat electrons in the solar wind, interstellar blast waves, and hot gas permeating galaxy clusters. How much shock heating goes to electrons instead of ions, and what plasma physics controls electron heating? We simulate 2-D perpendicular shocks with a fully kinetic particle-in-cell code. For magnetosonic Mach number $\mathcal{M}_\mathrm{ms} \sim 1$-$10$ and plasma beta $β_\mathrm{p} \lesssim 4$, the post-shock electron/ion temperature ratio $T_\mathrm{e}/T_\mathrm{i}$ decreases from $1$ to $0.1$ with increasing $\mathcal{M}_\mathrm{ms}$. In a representative $\mathcal{M}_\mathrm{ms}=3.1$, $β_\mathrm{p}=0.25$ shock, electrons heat above adiabatic compression in two steps: ion-scale $E_\parallel = \vec{E} \cdot \hat{b}$ accelerates electrons into streams along $\vec{B}$, which then relax via two-stream-like instability. The $\vec{B}$-parallel heating is mostly induced by waves; $\vec{B}$-perpendicular heating is mostly adiabatic compression by quasi-static fields.