Effect of weak bases on the intralysosomal pH in mouse peritoneal macrophages
B. Poole, S. Ohkuma
The spectral characteristics of dextran, labeled with fluorescein, depend upon pH. We have loaded the lysosomes of mouse peritoneal macrophages with this fluorescence probe and used it to measure the intralysosomal pH under various conditions. The pH of the medium has no effect on the intralysosomal pH. Weakly basic substances in the medium cause a concentration-dependent increase in the intralysosomal pH. However, the concentration of base necessary to produce a significant change in the intralysosomal pH varies over a wide range for different bases. The active form of the base is the neutral, unprotonated form. Although most of these weak bases cause an increase in the volume of the lysosomes, increase in lysosomal volume itself causes only a minor perturbation of the intralysosomal pH. This was demonstrated in cells whose lysosomes were loaded with sucrose, and in cells vacuolated as a demonstrated in cells whose lysosomes were loaded with sucrose, and in cells vacuolated as a consequence of exposure to concanavalin A. The results of these studies are interpreted in terms of energy-dependent lysosomal acidification and leakage of protons out of the lysosomes in the form of protonated weak bases.
719 sitasi
en
Medicine, Biology
Two‐dimensional electrophoresis. The current state of two‐dimensional electrophoresis with immobilized pH gradients
A. Görg, W. Postel, S. Günther
Changes in the conformation of influenza virus hemagglutinin at the pH optimum of virus-mediated membrane fusion.
J. Skehel, P. Bayley, E. Brown
et al.
696 sitasi
en
Medicine, Chemistry
NITROGEN ASSIMILATION AND TRANSPORT IN VASCULAR LAND PLANTS IN RELATION TO INTRACELLULAR pH REGULATION
J. Raven, F. A. Smith
A Scattered-Field Formulation for Coupled Geometric Wakefield and Space Charge Field Simulations in Particle Accelerators
J. Christ, E. Gjonaj, H. De Gersem
We propose a self-consistent simulation model for particle beams in accelerators, which includes the impact of electromagnetic wakefields caused by the geometry of the accelerator chamber. The method is based on a scattered-field formulation for the beam-driven Maxwell's equations. The total electromagnetic field seen by the particles is obtained as the solution of two coupled problems: a purely wakefield problem and a space charge field problem, where for each of these problems, specialized and numerically efficient approaches can be used. To assess the accuracy of the method, we compare simulation results with the analytical solution for a relativistic beam in a uniform accelerator pipe. The numerical efficiency of the method is, furthermore, demonstrated in the beam dynamics study of the multi-cell RF photo-gun installed at the SuperKEK collider facility. We show that electromagnetic wakefields have a non-negligible impact on the quality of the generated beam and, therefore, should be taken into account in the design of high-brilliance electron sources.
en
physics.comp-ph, physics.acc-ph
Metabolic regulation via intracellular pH.
W. Busa, R. Nuccitelli
665 sitasi
en
Medicine, Biology
Na+/H+ exchange and cytoplasmic pH in the action of growth factors in human fibroblasts
W. Moolenaar, R. Tsien, P. Saag
et al.
636 sitasi
en
Chemistry, Medicine
pH-independent HIV entry into CD4-positive T cells via virus envelope fusion to the plasma membrane.
B. S. Stein, B. S. Stein, S. D. Gowda
et al.
635 sitasi
en
Medicine, Biology
Self-seeded photon acceleration by electron beam-driven transition radiation
Chaolu Ding, Xuesong Geng, Liangliang Ji
Photon acceleration (PA) driven by ultra-relativistic electron beams offers a promising approach to generating high-power, high-frequency coherent radiation sources. While current methods typically rely on external optical laser pulses injected into beam-driven plasma wakefields, they face significant challenges in synchronization and alignment between electron accelerators and laser systems. We propose utilizing transition radiation (TR) generated by the drive electron bunch transversing the vacuum-gas interface as the seed photons of PA. Using a 1 GeV electron bunch, we demonstrate acceleration of TR from 4.4 μm to 184 nm in 1.6 mm of two-stage uniform plasma, achieving more than a 20-fold frequency boost. Further frequency increases can be achieved with optimized setups. This scheme addresses the synchronization and alignment issues present in previous approaches, providing a practical path toward beam-driven photon acceleration.
en
physics.plasm-ph, physics.acc-ph
Regulation of intracellular pH in eukaryotic cells.
I. H. Madshus
608 sitasi
en
Chemistry, Medicine
Topical Issue "Dynamics of Systems on the Nanoscale (2021)". Editorial
Alexey V. Verkhovtsev, Vincenzo Guidi, Nigel J. Mason
et al.
Exploration of the structure formation and dynamics of animate and inanimate matter on the nanometer scale is a highly interdisciplinary field of rapidly emerging research. It is relevant for various molecular and nanoscale systems of different origins and compositions and concerns numerous phenomena originating from physics, chemistry, biology, and materials science. This topical issue presents a collection of research papers devoted to different aspects of the Dynamics of Systems on the Nanoscale. Some of the contributions discuss specific applications of the research results in several modern and emerging technologies, such as controlled nanofabrication with charged particle beams or the design and practical realization of novel gamma-ray crystal-based light sources. Most works presented in this topical issue were reported at the joint Sixth International Conference "Dynamics of Systems on the Nanoscale" and the tenth International Symposium "Atomic Cluster Collisions" (DySoN-ISACC 2021), which were held in Santa Margherita Ligure, Italy, in October 2021.
en
physics.chem-ph, cond-mat.mes-hall
Ionic-strength and pH effects on the sorption of cadmium and the surface charge of soils
R. Naidu, N. Bolan, R. Kookana
et al.
Radiation reaction-dominated regime of wakefield acceleration
A. A. Golovanov, E. N. Nerush, I. Yu. Kostyukov
We study electron acceleration in a plasma wakefield under the influence of the radiation-reaction force caused by the transverse betatron oscillations of the electron in the wakefield. Both the classical and the strong quantum-electrodynamic (QED) limits of the radiation reaction are considered. For the constant accelerating force, we show that the amplitude of the oscillations of the QED parameter $χ$ in the radiation-dominated regime reaches the equilibrium value determined only by the magnitude of the accelerating field, while the averaged over betatron oscillations radiation reaction force saturates at the value smaller than the accelerating force and thus is incapable of preventing infinite acceleration. We find the parameters of the electron bunch and the plasma accelerator for which reaching such a regime is possible. We also study effects of the dephasing and the corresponding change of accelerating force over the course of acceleration and conclude that the radiation-dominated regime is realized both in cases of single-stage acceleration with slow dephasing (usually corresponding to bunch-driven plasma accelerators) and multi-stage acceleration with fast dephasing (corresponding to the use of laser-driven accelerators).
en
physics.plasm-ph, physics.acc-ph
Direct laser-driven electron acceleration and energy gain in helical beams
Etele Molnár, Dan Stutman
A detailed study of direct laser-driven electron acceleration in paraxial Laguerre-Gaussian modes corresponding to helical beams $\text{LG}_{0m}$ with azimuthal modes $m=\left\{1,2,3,4,5\right\}$ is presented. Due to the difference between the ponderomotive force of the fundamental Gaussian beam $\text{LG}_{00}$ and helical beams $\text{LG}_{0m}$ we found that the optimal beam waist leading to the most energetic electrons at full width at half maximum is more than twice smaller for the latter and corresponds to a few wavelengths $Δw_0=\left\{6,11,19\right\}λ_0$ for laser powers of $P_0 = \left\{0.1,1,10\right\}$ PW. We also found that for azimuthal modes $m\geq 3$ the optimal waist should be smaller than $Δw_0 < 19 λ_0$. Using these optimal values we have observed that the average kinetic energy gain of electrons is about an order of magnitude larger in helical beams compared to the fundamental Gaussian beam. This average energy gain increases with the azimuthal index $m$ leading to collimated electrons of a few $100$ MeV energy in the direction of the laser propagation.
en
physics.plasm-ph, physics.acc-ph
Polymeric anticancer drugs with pH-controlled activation.
K. Ulbrich, V. Šubr
490 sitasi
en
Chemistry, Medicine
pH-responsive supramolecular nanovalves based on cucurbit[6]uril pseudorotaxanes.
S. Angelos, Yingwei Yang, K. Patel
et al.
420 sitasi
en
Medicine, Chemistry
Spin polarized proton beam generation from gas-jet targets by intense laser pulses
Luling Jin, Meng Wen, Xiaomei Zhang
et al.
A method of generating spin polarized proton beams from a gas jet by using a multi-petawatt laser is put forward. With currently available techniques of producing pre-polarized monatomic gases from photodissociated hydrogen halide molecules and petawatt lasers, proton beams with energy ~ 50 MeV and ~ 80 % polarization are proved to be obtained. Two-stage acceleration and spin dynamics of protons are investigated theoretically and by means of fully self-consistent three dimensional particle-in-cell simulations. Our results predict the dependence of the beam polarization on the intensity of the driving laser pulse. Generation of bright energetic polarized proton beams would open a domain of polarization studies with laser driven accelerators, and have potential application to enable effective detection in explorations of quantum chromodynamics.
en
physics.plasm-ph, physics.acc-ph
PYG4OMETRY: a Python library for the creation of Monte Carlo radiation transport physical geometries
Stewart Boogert, Andrey Abramov, Laurence Nevay
et al.
Creating and maintaining computer readable geometries for use in Monte Carlo Radiation Transport (MCRT) simulations is an error-prone and time-consuming task. Simulating a system often requires geometry from different sources and modelling environments, including a range of MCRT codes and computer-aided design (CAD) tools. PYG4OMETRY is a Python library that enables users to rapidly create, manipulate, display, read and write Geometry Description Markup Language (GDML)-based geometry used in simulations. PYG4OMETRY provides importation of CAD files to GDML tessellated solids, conversion of GDML geometry to FLUKA and conversely from FLUKA to GDML. The implementation of PYG4OMETRY is explained in detail along with small examples. The paper concludes with a complete example using most of the PYG4OMETRY features and a discussion of extensions and future work.
en
physics.comp-ph, hep-ex
Biomimetic pH Sensitive Polymersomes for Efficient DNA Encapsulation and Delivery
Hannah Lomas, I. Cantón, S. MacNeil
et al.
421 sitasi
en
Materials Science
Transverse instability in the "light sail" ion acceleration
Y. Wan, I. A. Andriyash, W. Lu
et al.
Acceleration of ultrathin plasma foils by laser radiation pressure promises compact alternatives to the conventional ion accelerators. It was shown, that a major showstopper for such schemes is a strong transverse instability, which develops the surface ripples, and is often attributed to the Rayleigh-Taylor (RT) type. However, simulations indicate, that these perturbations develop the features, that cannot be consistently explained by the RT mechanism. Here we develop a three-dimensional (3D) theory of this instability, which shows that its linear stage is mainly driven by strong electron-ion coupling, while the RT contribution is actually weak. Our model provides the instability spectral structure and its growth rate, that agrees with the large scale 3D particle-in-cell simulations. Numerical modeling shows, that target destruction results from a rapid plasma heating induced by the instability field. Possible paths to instability mitigation are discussed.
en
physics.plasm-ph, physics.acc-ph