T. Citarasu
Hasil untuk "Environmental effects of industries and plants"
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Anco S. Blazev
Factors, both human and physical, including climatic conditions, hydrology, geomorphology and cropping practices are evaluated in terms of their contribution in desertification development. Particular emphasis is given on the effects of cultivation practices and the animal husbandry. The relation between local plants and desertification is also studied, emphasizing the role of the olive tree which is the dominant crop in the area. The main causes which are found to favor the occurrence of desertification include population increase, the extent of areas with low or without plant coverage, overgrazing combined or with the occurrence of fires, and the increased content in salts of water resources, especially at the eastern part of the island. With regard to agricultural practices and especially these applied for olive oil trees environmental compacts are either positive or negative depending on the physical circumstances and the used cultivating practices. What is important is the way weeds are combated, the type of the soil, irrigation applied and the conservation of plant cover. Vegetation restoration is considered one of the most effective ways to combat desertification. Native plant species suitable for such use include Ceratonia siliqua, Olea europea subsp.sylvestri and Pistacia lentiscus which are adapted to environmental conditions prevailing in the island of Crete. Additionally, findings from the international literature reveal an important role of AM fungi on the establishment of vegetation in degraded soils. Understanding the interrelationships between vegetation and soil microfauna is expected to contribute in the successful reestablishment of native shrub species in the degraded soils in Crete. Brief Biography of the Speaker: Vassilis Gekas son of Christos and Dimitra was born in Gallini of Larissa the year 1948. His studies were as follows: elementary school in the Gallini village (1954-1960) , secondary school in Larissa και(1960-1966). Chemical Engineering School in the National Technical University of Athens, (1966-1971). Military Service 1971-1973. In the Greek Industry worked as a Chemical Engineer (1973-1983) Ph D studies in the Technical Institute of Lund (1983-1987). Post doc studies on an ELF AQUITAINE sponsorship in the PAUL SABATIER University in Toulouse of France. Coming back to Sweden he obtained the degree of DOCENT (Asssociate Professor) in 1992. He taught in Lund and HELSINGBORG of Sweden, Oporto Portugal, Bahia Blanca and Rio Cuarto og Argentina, Celaya and Mexico City of Mexico and also he gave lectures in France, Italy, Spain and Germany. He speaks english, french, german, spanish, italian, portuguese and swedish. Since 1998 Professor of Transport Phenomana & Thermodynamics at the Environmental Department of the Technical University of Crete, Chania. He was the first Chairman of the Department (2000-2004). Author of several books and of chapters of books, in english and in greek. Author of >50 publications in cited journals with a Citation Index of approx. 1000. Know how in Membrane Technology, Food Engineering, Stirling cycles and active heliothermic systems. Current research topics: Desertification, Geometry & transport phenomena of biological structures such as the bronchic tree, sun-driven cars, BOLTZMANN equation. He participates in the Summer school of Delfi teaching on the connection between the modern knowledge with the Ancient Greek wisdom. 2nd WSEAS/IASME International Conference on RENEWABLE ENERGY SOURCES (RES'08) Corfu, Greece, October 26-28, 2008 ISSN: 1790-5095 11 ISBN: 978-960-474-015-4
Parul Chaudhary, M. Xu, L. Ahamad et al.
Climate change, inadequate possessions, and land degradation all pose obstacles to modern agriculture. In the current scenario, the agriculture industry is mainly dependent on the use of chemical-based pesticides and fertilizers that impact soil health and crop productivity. Moreover, water scarcity leads farmers in drastically affected regions to use heavy metal-enriched water sources mainly originating from industrial sources for field crops irrigation. Soil pollutants can be carried into the human body via dust and water, creating negative health effects varying from simple symptoms, e.g., nausea and diarrhea and reaching death in critical cases. Thus, to clean soil contaminants, and improve soil fertility and agricultural production, alternatives to chemical fertilizers must be developed. Therefore, using beneficial microbes found in plant-associated soil microorganisms offers an effective strategy to alleviate some of these challenges, improving soil fertility, and crop yield, and protecting plants from stress conditions. Through the use of synergistic interactions, the synthetic consortium strategy seeks to improve the stability of microbial communities. In this review, synthetic consortia and their potential use in agriculture were discussed. Further, engineering new effective synthetic consortia was suggested as an effective approach in the concept of environmental bioremediation of soil pollutants and contaminants.
Xuanru Zhou, Gengyuan Liu, Hongxiu Li et al.
Rare earth elements (REEs) are indispensable raw materials for green technologies, but their production process may cause significant ecological pressure. As the world's major supplier of REEs, China undertakes most of the mining and refining tasks, but it is unclear whether the Chinese rare earth supply chain has an impact on biodiversity and what part is due to foreign consumption and what part is domestic consumption. Here, we developed BLARES, a dynamic assessment framework that combines system dynamics with life cycle biodiversity impact accounting, to evaluate the impact of China's rare earth supply chain on biodiversity during the period from 2000 to 2060 under four SSP scenarios. We used mean species abundance (MSA) to measure the impact, covering land occupation and climate-driven pathways, and considering ecological time lag factors. The results show a clear ecological debt pattern. In the medium term, biodiversity pressure increases or remains high because the marginal effects brought by upstream expansion are released. Only when the used inventory of green technology products in rare earths is accumulated to stabilize the replacement of fossil energy, will the impact of the rare earth supply chain on biodiversity show a net improvement. Compared to the baseline value in 2000, the cumulative avoided biodiversity loss has exceeded 4 × 107 MSA·ha·yr by 2060. The net impact turns positive around 2040, and the cumulative net impact reaches zero around 2049, indicating a significant delay in ecological compensation. The time when the carbon balance occurs is earlier than that of biodiversity compensation, suggesting that net zero carbon emissions do not necessarily mean zero ecological emissions. From the perspective of final demand attribution, for every 1% increase in overseas demand, China's annual net biodiversity loss increases by approximately 0.031%, while for every 1% increase in the use of rare earth elements for green deployment in China, it decreases by about 0.84%. Our research results do not support the simplistic claim that “the more deployment, the better”. The transition path should be evaluated using the time of ecological compensation and the peak of medium-term pressure, rather than solely based on the final carbon emission results.
Harith Sadaa Madhan Al-Fahdawy, Mohammed Ismail Khalaf Al-Fahdawy, Omer Ismail Al-Fahdawi and Amer Hashim Abdulmajeed
Two laboratory experiments were conducted in the Desert Studies Center laboratories during the spring season of 2024 to evaluate the efficiency of Cement Kiln Dust (CKD) in removing lead and cadmium from polluted water. The first experiment represents the thermally isotropic adsorption of heavy metals on the cement kiln dust (CKD) surface. The experiment included three diameters of CKD particles, i.e., 0.3, 1.18, and 2 mm, treated with four concentrations of cadmium and lead, namely 20, 40, 80, and 160 mg.L⁻¹ for each element (cadmium and lead). The amount of the adsorbed metals on the surface of CKD was calculated according to the Langmuir equation. In contrast, the second experiment represents the effect of contact time between the heavy metals and CKD particles for the same aforementioned diameters and concentrations. The results of the isothermal adsorption experiment showed that the adsorbed amount increased with increasing the added concentrations of heavy metals. The adsorbed quantity of cadmium is superior to that of lead. Also, the adsorption capacity of cadmium was higher compared to the lead adsorption capacity. In this context, the adsorption capacity reached 2880.00 and 2735.58 mg.kg⁻¹ for cadmium and lead, respectively. Regarding the second experiment, the results showed that the amount of cadmium and lead adsorbed on the CKD particle’s surface increased with time, where the highest amount of cadmium and lead adsorption was 39.94 and 34.93%, respectively, for shaking of 4 h. It is recommended to apply the experiment in real-world projects.
José M. Santiago, Diego García de Jalón
Water temperature is a key ecological and metabolic factor in rivers and other continental systems, and thermal pollution caused by human activities (dams, discharges, urban stormwater, industrial cooling) alters the natural thermal regime of rivers, modifying the structure and functioning of communities (primary producers, macroinvertebrates and fish) and favouring thermophilic and often invasive species. Wastewater treatment plants (WWTPs) generate and discharge excess heat: their effluents are often several degrees above the temperature of the receiving river, which increases the metabolism of communities, favours eutrophication and can intensify the effects of nutrients and toxic pollutants. This excess heat from wastewater is a major renewable energy resource that can be recovered using heat pumps, both in buildings and in the treatment plants themselves, as well as in district heating networks, reducing the demand for fossil fuels and CO₂ emissions. Heat recovery in WWTPs, especially from treated effluent connected to district networks, offers very high technical potential (tens of TWh per year on a national scale in some countries) and can contribute significantly to more sustainable urban energy systems. Heat recovery in WWTPs can minimise the thermal impact of effluents on receiving rivers, reducing the negative effects of discharges on the natural environment.
A. Sirico, P. Bernardi, C. Sciancalepore et al.
Kelvin Wade Ivanković, Natalia Maehle
Despite significant public and academic interest in the sharing economy (SE), the concept remains underexplored in the business-to-business (B2B) context. By facilitating access to shared resources, the B2B SE can create new value for businesses and offer novel insights for management theory and practice. This study draws on business network, ecosystem, and value co-creation research to investigate how value is co-created in the B2B SE by taking an ecosystem perspective. A case study methodology is employed, utilising the Ecosystem Pie Model as a framework for analysis. The selected case operates as a B2B resource-sharing facilitator for businesses in the ocean industries in Bergen, Norway. Data comprises 17 stakeholder interviews, 35 documents, and 13 h of observations. Through our ecosystem analysis, we provided insight into the coexistence and interdependence of a diverse range of actors, including the B2B SE facilitator, resource providers, resource users, third-party service providers, public authorities and investors. Our findings reveal how the B2B SE fosters the co-creation of shared value by creating economic benefits for businesses and environmental benefits for society. However, identified challenges (including regulatory challenges and power imbalances) may prevent the B2B SE ecosystem from fully delivering on its value proposition. We highlight the crucial role of the B2B SE facilitator in mitigating ecosystem risks and challenges while orchestrating key ecosystem activities such as matchmaking, contracting, and relationship management. This study contributes to the SE literature from a B2B ecosystem perspective, providing a foundation for future research and practical insights for businesses, SE facilitators, and policymakers.
Riya Kumbukattu Alex, Nimitha Aboobaker, Suja Purushothaman Devipriya
Plastic microbeads in personal care products have raised significant environmental concerns, prompting regulatory interventions worldwide. However, consumer intentions play a crucial role in ensuring the success of such initiatives, particularly in emerging economies where market diversity and socio-cultural complexities exist. This study developed an integrative model combining the Theory of Planned Behaviour, Value Belief Norms, and Social Cognitive Theory to understand the pro-environmental behavioural intentions of Indian consumers towards microplastics in personal care products. A nationwide survey (N = 375) and Structural Equation Modelling (SEM) with PROCESS macro analysis were conducted. The study identified ‘moral values’ as a critical determinant of Behavioural Intention, influencing decisions through two indirect multiple mediation pathways; the former involving ‘self-efficacy’ and ‘attitude’ (β = 0.119), whereas the latter includes ‘environmental knowledge’ and ‘attitude’ (β = 0.061). In this study, ‘observational learning’ exhibited a novel conditional moderation effect at moderate and high levels through ‘self-efficacy’ (β = 0.055) and ‘perceived behavioural control’ (β = 0.034), emphasising the role of social learning in shaping consumers' intentions towards purchases. A baseline observation of this study noticed that younger, female, and scientifically educated consumers were more emotionally responsive and supportive of regulatory measures, while older and non-science background consumers preferred incentive-based approaches. The insights from this study will aid in developing responsible consumption patterns through targeted awareness campaigns, eco-labelling, influencer-led promotions, and educational curriculum reforms, which are linked to Sustainable Development Goal 12.
Rika Harini, Ismi Puspitaningrum, Vanessa Anggreta Sari et al.
The economic conversion of agricultural land often disregards its environmental significance, threatening food security and ecosystem integrity. This study evaluated the potential of agricultural land resources within the Sustainable Food Agricultural Land Area (LP2B) in the Special Region of Yogyakarta Province, Indonesia, by quantifying their total economic value (TEV) and developing strategic recommendations for sustainable land management. Utilizing a mixed-method approach based on secondary data and SWOT analysis, the research revealed that Bantul Regency is the regency with the highest total economic value of agricultural land, and Gunungkidul is the regency with the lowest total economic value, exhibiting high TEV due to its robust agricultural productivity and ecological functions. The study emphasized indirect benefits—including flood and erosion control—that are undervalued in current land-use decision-making. Strategic solutions such as digital agriculture initiatives and farmer regeneration programs are proposed to mitigate land conversion and bolster rural resilience. These findings provide actionable insights for policymakers aiming to balance economic development and environmental sustainability in rapidly urbanizing regions.
Wei Chen, Xiquan Zhao, Hu Yu
Titanium is a crucial raw material in the chemical industry, metallurgy, machinery manufacturing, aerospace, and other fields. Evaluating the spatiotemporal dynamics of global titanium trade patterns is essential for a comprehensive understanding of the titanium trade situation and for enhancing the stability of the titanium supply chain. To this end, this paper constructs global trade networks of titanium ore, titanium dioxide, titanium sponge, and titanium products and utilizes various network analysis methods to deeply investigate the spatiotemporal evolution of global titanium trade patterns, the topology of the trade network, and the resilience characteristics. The study reveals a growing global titanium trade with fluctuations, significantly clustered trade flows, and expanding, denser networks with optimized structures. Over the past 20 years, the trade volumes of titanium ore, titanium dioxide, and titanium products have increased to approximately 2.93, 1.80, and 1.90 times their previous levels, respectively. The global titanium trade networks present significant core–periphery structure characteristics. The centralization coefficients of the four types of titanium commodity trade networks all exceed 0.9, with clear boundaries between the core and peripheral layers, as well as differences in connectivity across the various layers of these networks. The resilience of the global titanium trade network has increased over time, with the resilience of the titanium dioxide and titanium products trade networks significantly higher than that of titanium ore and titanium sponge. However, the failure of key nodes such as China, the United States, and Germany still has a greater impact on network connectivity, and the overall ability of the network to resist intentional attacks is still limited. Finally, this paper puts forward policy recommendations to promote titanium trade cooperation, enhance the resilience of the titanium trade network, and maintain the security of the titanium supply.
Mariana A Andrade, Ana Sanches-Silva, Hooman Chodar Moghadas et al.
Increasing environmental concerns over using petroleum-based packaging materials in the food industry have encouraged researchers to produce edible food packaging materials from renewable sources. Biopolymer-based edible films and coatings can be implemented as bio-based packaging materials for prolonging the shelf life of food products. However, poor mechanical characteristics and high permeability for water vapor limit their practical applications. In this regard, plant oils (POs) as natural additives have a high potential to overcome certain shortcomings related to the functionality of edible packaging materials. In this paper, a summary of the effects of Pos as natural additives on different properties of edible films and coatings is presented. Moreover, the application of edible films and coatings containing POs for the preservation of different food products is also discussed. It has been found that incorporation of POs could result in improvements in packaging’s barrier, antioxidant, and antimicrobial properties. Furthermore, the incorporation of POs could significantly improve the performance of edible packaging materials in preserving the quality attributes of various food products. Overall, the current review highlights the potential of POs as natural additives for application in edible food packaging materials.
A. Yashwanth, Rundong Huang, Monica Iepure et al.
Abstract Food packaging (FP) is essential for preserving food quality, safety, and extending shelf‐life. However, growing concerns about the environmental and health impacts of conventional packaging materials, particularly per‐ and polyfluoroalkyl substances (PFAS) and microplastics, are driving a major transformation in FP design. PFAS, synthetic compounds with dual hydro‐ and lipophobicity, have been widely employed in food packaging materials (FPMs) to impart desirable water and grease repellency. However, PFAS bioaccumulate in the human body and have been linked to multiple health effects, including immune system dysfunction, cancer, and developmental problems. The detection of microplastics in various FPMs has raised significant concerns regarding their potential migration into food and subsequent ingestion. This comprehensive review examines the current landscape of FPMs, their functions, and physicochemical properties to put into perspective why there is widespread use of PFAS and microplastics in FPMs. The review then addresses the challenges posed by PFAS and microplastics, emphasizing the urgent need for sustainable and bio‐based alternatives. We highlight promising advancements in sustainable and renewable materials, including plant‐derived polysaccharides, proteins, and waxes, as well as recycled and upcycled materials. The integration of these sustainable materials into active packaging systems is also examined, indicating innovations in oxygen scavengers, moisture absorbers, and antimicrobial packaging. The review concludes by identifying key research gaps and future directions, including the need for comprehensive life cycle assessments and strategies to improve scalability and cost‐effectiveness. As the FP industry evolves, a holistic approach considering environmental impact, functionality, and consumer acceptance will be crucial in developing truly sustainable packaging solutions.
Patil Pramod Sambhaji
Haohao Li, Lin Huo, Rui Zhang et al.
Soil is the place where human beings, plants, and animals depend on for their survival and the link between the various ecological layers. Groundwater is an important component of water resources and is one of the most important sources of water for irrigated agriculture, industry, mining and cities because of its stable quantity and quality. Soil and groundwater are important strategic resources highly valued by countries around the world. However, in recent years, the deterioration of the ecological environment of soil-groundwater caused by industrial, domestic, and agricultural pollution sources has continued to threaten human health and ecological security. Among them, organochlorine pesticides (OCPs), as typical organic pollutants, cause very serious pollution of soil and groundwater environment. However, most studies on the pollution of OCPs have focused on the aboveground or surface water environment, and little consideration has been given to the pollution and hazards of OCPs to the deep soil and groundwater environment, especially the effects of different environmental factors on the transport and transformation of OCPs in soil-groundwater. Moreover, in addition to the influence of a single factor on it, the interactions that arise between different factors cannot be ignored. This paper focuses on two major sources of OCPs in soil and groundwater environments, compiles and summarizes the effects of environmental factors such as pH, microbial communities and enzyme activities on the transport and transformation of OCPs in soil and groundwater systems, discusses the synergistic effects of individual environmental factors and others, and comprehensively analyses the effects of synergistic effects of various environmental factors on the transport and transformation of OCPs. In the context of ecological civilization construction, it provides the scientific basis and theoretical foundation for the prevention and treatment of OCPs-contaminated soil and groundwater, and puts forward new ideas and suggestions for the research and development of green, eco-friendly remediation and treatment technologies for OCPs-contaminated sites.
K. Kumawat, N. Razdan, K. Saharan
In the light of intensification of cropping practices and changing climatic conditions, nourishing a growing global population requires optimizing environmental sustainability and reducing ecosystem impacts of food production. The use of microbiological systems to ameliorate the agricultural production in a sustainable and eco-friendly way is widespread accepted as a future key-technology. However, the multitude of interaction possibilities between the numerous beneficial microbes and plants in their habitat calls for systematic analysis and management of the rhizospheric microbiome. This review exploits present and future strategies for rhizospheric microbiome management with the aim to generate a comprehensive understanding of the known tools and techniques. Significant information on the structure and dynamics of rhizospheric microbiota of isolated microbial communities is now available. These microbial communities have beneficial effects including increased plant growth, essential nutrient acquisition, pathogens tolerance, and increased abiotic as well as biotic stress tolerance such as drought, temperature, salinity and antagonistic activities against the phyto-pathogens. A better and comprehensive understanding of the various effects and microbial interactions can be gained by application of molecular approaches as extraction of DNA/RNA and other biochemical markers to analyze microbial soil diversity. Novel techniques like interactome network analysis and split-ubiquitin system framework will enable to gain more insight into communication and interactions between the proteins from microbes and plants. The aim of the analysis tasks leads to the novel approach of Rhizosphere microbiome engineering. The capability of forming the rhizospheric microbiome in a defined way will allow combining several microbes (e.g. bacteria and fungi) for a given environment (soil type and climatic zone) in order to exert beneficial influences on specific plants. This integration will require a large-scale effort among academic researchers, industry researchers and farmers to understand and manage interactions of plant-microbiomes within modern farming systems, and is clearly a multi-domain approach and can be mastered only jointly by microbiology, mathematics and information technology. These innovations will open up a new avenue for designing and implementing intensive farming microbiome management approaches to maximize resource productivity and stress tolerance of agro-ecosystems, which in return will create value to the increasing worldwide population, for both food production and consumption.
Sibange Paul, Barihun Thyrniang, Sumit Deb et al.
Organic compounds and pollutants from industries like oil refineries, such as total petroleum hydrocarbon (TPH) and polycyclic aromatic hydrocarbons (PAHs) are known to pose toxic effects to the environment by degrading the soil and water quality along with the deposition of heavy metals thereby causing a threat to the life forms existing in them. This pose of imbalance in the ecosystem calls for an exigent notice and effort regarding controlling it. Hydrocarbon sludge from Effluent Treatment Plant (ETP) is one such toxic effluent eluted from oil refineries, and that is yet to be reported for a biodegradation study. Among a huge number of physical and chemical techniques, bioremediation has been a much talked about measure but is not in the required scale of practice yet. The reason could be a lack of efficient standardization for environmental applications. Cyanobacteria being well-known for their ability to survive difficult environmental conditions efficiently and to adapt to a mixotrophic nature, makes them ideal for performing bioremediation at a large scale in the open environment. The study aimed to quantify this ability of cyanobacteria by assessing the TPH content of the treatment sample, Effluent Treatment plant (ETP) hydrocarbon sludge pre- and post-treatment using GC-FID. The study was designed to treat the sludge by cyanobacterial strains in a pre-determined lethal dose concentration and monitor the activity of enzymes vital for a basic degradation metabolism, in addition to the growth rates of the cultures. The figures obtained from the enzyme assays and the growth rates appeared to be collateral in the direction of it being a highly promising bioremediation approach that could be efficiently performed by increasing the scale of application. The chromatograms obtained from the GC-FID depicted significant reductions in the TPH content of the treated samples that strongly indicated the potential of the cyanobacterial cultures to bioremediate an oil- contaminated site or treat toxic effluents from oil refineries.
Dwi Ariyani, Muh. Yanuar Jarwadi Purwanto, Euis Sunarti et al.
Floods are one of the most prevalent disasters worldwide, including in the capital city of Indonesia. Multi-criteria decision analysis is combined with Geographic Information Systems to produce a mapping of flood hazard areas. The weighting for each parameter is based on six criteria: rainfall, slope gradient, topography, soil type, land cover, and distance from rivers. The flood hazard map is validated using inundation data from the Regional Disaster Management Agency for the years 2015 and 2020. From the general analysis, it can be determined that the parameter most influencing floods is rainfall with a weight of 0.270, followed by slope gradient at 0.164, topography at 0.124, soil type at 0.096, land cover at 0.190, and distance from rivers at 0.155. Therefore, through mapping using QGIS, it is revealed that in 2002, highly flood hazard areas comprised 20.99% of the total Ciliwung Watershed area, which increased to 24.31% in 2020. The validation of the flood hazard map was conducted by recording the coordinate points of flood incidents in 2015 and 2020, revealing that the affected areas within the Ciliwung Watershed occurred in high to very high vulnerability zones. This research demonstrates that flood events in the study area occurred in high to very high flood hazard zones. The results of this study are considered valuable and important for providing accurate information to local governments to develop cost-effective and efficient strategies in dealing with potential flood hazards.
S. Piyavadee, R. Chumaporn and V. Patipat
The most significant air pollutant is particulate matter of less than 10 microns (PM10), followed by ozone (O3) during the monitoring period from 2006 to 2022 in Chiangmai. The association between ambient pollutants and climate conditions in Chiangmai was assessed using regression analysis and analysis of variance (ANOVA). The ANOVA analysis indicated that the average temperature was associated significantly with the nitrogen dioxide (NO2) concentration in the ambient, but the average rainfall volume was associated significantly with most pollutants except only sulfur dioxide (SO2). From the prediction models, the rise in average temperature affected to increase in the concentrations of PM10 and O3. Interestingly, the increase in rainfall will be advantageous to compromise the severity of all pollutants. Meanwhile, on hotter days should be careful of the rise of PM10 and O3 concentrations. Therefore, the vital meteorological variables associated with air pollution are very useful for forecasting the harmful and severity level of each air pollutant.
Shanhu Ma, Mengjie Wang, Peng Li et al.
Cymbidium floribundum is an ornamental plant with showy and colorful flowers. The color of its lip changes significantly after pollination. However, the mechanism of lip coloration remains unclear. In this study, the mechanism underlying lip color change in C. floribundum was investigated before and after pollination. Metabolome analysis detected 61 flavonoids in the lip, including 24 flavonoids, 13 flavonols, nine flavonoid carbonosides, eight anthocyanins, three flavanols, two isoflavones, one chalcone, and one dihydroflavone. Accumulation of peonidin 3-O-glucoside chloride, cyanin chloride, and cyanidin 3-O-malonylhexoside after pollination may be the key factors contributing to the change in lip color. Furthermore, transcriptome analysis identified 43 genes related to the anthocyanin biosynthesis pathway (ABP). Phylogenetic and co-expression analysis indicated that CfMYB1, CfMYB3, and CfMYB4 may be involved in the regulation of anthocyanin biosynthesis in the lips. Subcellular localization results showed that CfMYB1 was located in the nucleus, while CfMYB3 and CfMYB4 were located in the nucleus and cytoplasm. Further functional analysis verified that CfMYB1 could activate ABP genes and promote the synthesis and accumulation of anthocyanin, which may be the main transcription factors leading to the change of lip color in C. floribundum after pollination. These findings provide insight into the anthocyanin accumulation and coloration mechanisms during C. floribundum flower development. The results provide genetic resources and a theoretical basis for the improvement and breeding of flower color in C. floribundum.
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