The field of 3D printing is continuing its rapid development in both academic and industrial research environments. The development of 3D printing technologies has opened new implementations in rapid prototyping, tooling, dentistry, microfluidics, biomedical devices, tissue engineering, drug delivery, etc. Among different 3D printing techniques, photopolymerization-based process (such as stereolithography and digital light processing) offers flexibility over the final properties of the 3D printed materials (such as optical, chemical, and mechanical properties) using versatile polymer chemistry. The strategy behind the 3D photopolymerization is based on using monomers/oligomers in liquid state (in the presence of photoinitiators) that can be photopolymerized (via radical or cationic mechanism) upon exposure to light source of different wavelengths (depending on the photoinitiator system). An overview of recent evolutions in the field of photopolymerization-based 3D printing and highlights of novel 3D print...
Department of Biochemistry and Biophysics, Curriculum in Genetics and Molecular Biology, Lineberger Comprehensive Cancer Center, University of North Carolina at Chapel Hill, North Carolina 27599-7295, USA; Howard Hughes Medical Institute, Division of Nucleic Acids Enzymology, Department of Biochemistry, University of Medicine and Dentistry of New Jersey, Robert Wood Johnson Medical School, Piscataway, New Jersey 08854, USA
Ludovica Giancotti, Sara Sorrenti, Lorenzo Marini
et al.
<b>Background:</b> Gestational diabetes mellitus (GDM) and periodontitis (PD) are chronic inflammatory conditions that may share metabolic and immune pathways. Evidence suggests an association between them, although results across studies remain inconsistent. This systematic review and meta-analysis evaluated the relationship between GDM and PD and examined whether GDM influences key periodontal parameters. <b>Methods:</b> A systematic search of PubMed/MEDLINE, Scopus, Web of Science, and Embase was conducted up to August 2025 following PRISMA guidelines. Observational studies comparing periodontal status in pregnant women with and without GDM were included. Periodontal status was assessed using probing pocket depth (PPD), clinical attachment loss (CAL), and bleeding on probing (BOP). Study quality was evaluated with the Newcastle–Ottawa Scale, and random-effects models were applied to estimate pooled associations. <b>Results:</b> Fifteen studies involving about 3800 pregnant women met the criteria. A significant association was found between GDM and PD (Odds Ratio [OR] 2.10; 95% Confidence Interval [CI] 1.64–2.69). No significant association emerged between GDM and gingivitis. Women with GDM showed increased BOP and higher PPD, indicating greater periodontal inflammation, while CAL did not significantly differ between groups. <b>Conclusions:</b> The findings support a significant association between GDM and periodontitis, suggesting that gestational hyperglycemia may enhance periodontal inflammation and early tissue changes. Incorporating periodontal screening into prenatal care may benefit maternal oral and metabolic health. Further longitudinal and interventional studies are needed to clarify causality and to explore whether periodontal therapy may help reduce risks linked to GDM.
Background: Dens invaginatus (DI) is also known as dilated composite odontome, dilated gestant odontome, dens in dente, invaginated odontome, and dents telescopes. Although DI most commonly affects the permanent maxillary lateral incisors, it can also affect the molars. The cause is unknown; nevertheless, one possible explanation is focal growth retardation or localized external pressure on certain areas of the tooth bud, which causes the enamel organ to invade the dental papilla during the morphodifferentiation stage. The invagination of enamel organ is hypothesized to enhance the risk of caries, pulpal pathology, and inflammation of periodontium. Traditional conservative endodontic therapy can be attempted successfully in mild invagination. DI with pulpal or periapical pathologies typically entails periapical surgery with a retrograde filling, and extraction is indicated in complicated cases. The purpose of this study was to report a unique case of Oehler’s type IIIB DI in a premolar, accompanied by a comprehensive literature review.Case Presentation: An 11-year-old female child visited the Pedodontics clinic with the chief complaint of swelling on the left side of the jaw. The left mandibular region was tender on palpation and firm in consistency upon extraoral examination. Intraoral examination revealed a decayed, discolored tooth in the second premolar region with intraoral sinus and pus discharge on the buccal side. Radiographic examination revealed incomplete root formation with invagination and radicular dilation, along with an indistinct canal anatomy indicative of Oehler’s type IIIB DI.Conclusion: Extraction was the treatment of choice in this case due to chronic periapical abscess with poor prognosis of the tooth. Dental practitioners should evaluate the case thoroughly for proper diagnosis and treatment.
Sirjana Dahal, Anju Khapung, Samarika Dahal
et al.
Background: Little is known about attitudes and practices regarding oral health of developing countries. This study aimed to determine the self-assessment of oral health status among adults residing at Chautara, Sindhupalchowk, Nepal.
Methods: A descriptive cross-sectional study was conducted among 440 adults selected by convenience sampling method. Interview using WHO oral health questionnaire for adults 2013 was taken after receiving informed consent. Data were entered in Microsoft Excel Sheet and analysed in SPSS version 24. Mean, standard deviation, frequency and percentage were calculated.
Results: Majority had positive assessment regarding their teeth (366, 83.18%) and gums (384, 87.27%). Many (417, 94.77%) cleaned their teeth ≥one times a day. Majority used toothbrush (434, 98.64%) and paste (411, 93.41%) for cleaning their teeth. Some, (184, 41.82%) visited a dentist at least once in their lifetime and the main reason was pain or trouble in teeth/gums/mouth (94, 51.09%). Very often, they faced problems chewing (31, 7.0%) and biting (27, 6.1%) foods. They mostly consumed tea with sugar (132, 30.0%) and biscuits, cakes and cream cakes (59, 13.4%) several times a day. Most of them smoked cigarettes every day (93, 21.1%), followed by tobacco chewing (67, 15.2%). In the past 30 days, 179 (40.7%) drank one or more drinks of alcohol.
Conclusions: The study findings showed that most of the adult residents of Chautara, had positive self-assessment of their oral health status. However, they faced many oral health problems which may be due to their unhealthy lifestyle suggesting need for oral health awareness programs.
Keywords: Adults; oral health; self-assessment.
Yaosen Chen, Ikuo Yonemitsu, Risa Usumi-Fujita
et al.
Abstract Background Although mandibular autorotation concept (MAC) surgery is a novel technique for addressing long-term stability issues in patients with mandibular retrusion and open bite accompanied by temporomandibular joint osteoarthritis (TMJOA), studies on MAC surgery in animal models and its mechanisms are scarce. This study aimed to develop an animal model for anterior open bite and investigate the underlying histological and molecular mechanisms of TMJ changes, with the expectation of contributing to the MAC surgery concept. Materials and methods Thirty-six, 5-weeks-old female Wistar rats were randomly divided into three groups: (1) Bite-raise group (2-mm resin added to the upper molars at 5 weeks of age) (2), Recovery group (resin added at 5 weeks of age and removed at 13 weeks of age), and (3) Control group (normal rats). Micro-CT imaging was used to assess 3D morphology and bone structure. Histological and immunohistochemical analyses were performed to evaluate the cartilage changes, bone cell differentiation, and extracellular matrix degradation. Gene expression of inflammatory factors (IL-1β, IL-6, TNF-α), TGF-β1, and Postn were measured by qPCR. Results At 13 weeks of age, both Bite-raise and Recovery groups exhibited TMJOA-like changes, including decreased cartilage thickness and increased inflammatory gene expression. At 21 weeks of age, the Recovery group showed improved bone volume, reduced osteoclast activity, and lower MMP-13 and Postn levels than the Bite-raise group. Conclusion These results suggest the successful development of an animal model for anterior open bite and demonstrate that MAC surgery facilitates the recovery of compromised condyles.
Genomic and mutational sequencing in malignant transformation of oral leukoplakia (OLK) has emergingly gained momentum. In this short communication, we identified 5 retrospective follow-up studies and 5 cross-section comparative studies on this issue using formalin-fixed paraffin-embedded tissues. Copy number alteration (CNA) was demonstrated to increase with the grade of oral dysplasia. CNA-based algorithms showed better prediction performances than histological grade in assessing the risk of OLK malignant transformation. Importantly, we conducted a pooled-analysis on the mutation frequencies of the common oral cancer driver genes extracted from individual studies. The most common mutation gene was found to be TP53 (26.26 %; 95 % confidence intervals (CI), 20.61–32.82 %), followed by NOTCH1 (23.23 %; 95%CI, 17.87–29.61 %), FAT1 (16.67 %; 95%CI, 12.08–22.52 %), and CDKN2A (10.61 %; 95%CI, 6.98–15.73 %). Collectively, it is promising to establish molecular subtyping and risk stratification of OLK patients using genomic and mutational sequencing. We recommend the well-designed studies with a larger OLK patient population with clinical endpoints using fresh or frozen tissues and matched optimal samples as controls in further investigations.
Background An increased level of interleukin-17A and interleukin-18 in the serum and intestinal mucosa of celiac disease patients reflecting the severity of villous atrophy and inflammation was documented. Thus, the objective of this study was to evaluate the concentrations of salivary-17A, interleukin-1 beta, and interleukin-18 in patients with celiac disease who are on a gluten-free diet, both with and without periodontitis, and to compare these levels with those in healthy individuals. Methods The study involved 23 participants with serologically confirmed celiac disease (CD) and 23 control subjects. The CD patients had been following a gluten-free diet (GFD) for a minimum of 1 year and had no other autoimmune disorders. The research involved collecting demographic data, conducting periodontal examinations, gathering unstimulated whole saliva, and performing enzyme-linked immunosorbent assays to measure salivary interleukin-17A, interleukin-1 beta, and interleukin-18 levels. Spearman’s correlation analysis was utilized to explore the relationships between CD markers in patients on a GFD and their periodontal clinical findings. Results The periodontal findings indicated significantly lower values in celiac disease patients adhering to a gluten-free diet compared to control subjects (p = 0.001). No significant differences were found in salivary IL-17A, IL-18, and IL-1B levels between celiac disease patients and control subjects. Nevertheless, the levels of all interleukins were elevated in periodontitis patients in both the celiac and control groups. The IL-1 Beta level was significantly higher in periodontitis patients compared to non-periodontitis patients in the control group (p = 0.035). Significant negative correlations were observed between serum IgA levels and plaque index (r = −0.460, p = 0.010), as well as gingival index (r = −0.396, p = 0.030) in CD patients on a gluten-free diet. Conclusion Celiac disease patients on gluten-free diet exhibited better periodontal health compared to control subjects. However, increased levels of salivary IL-17A, IL-18 and IL-1B levels were associated with periodontitis. Additionally, serum IgA level was significantly inversely associated with periodontitis clinical manifestations and with salivary inflammatory mediators in CD patients on GFD.
Neha Agrawal, Sarah Mariam, Narinder Dev Gupta
et al.
Objective: To identify the potential determinants of tobacco counseling implementation among oral health professionals in India. Material and Methods: A cross-sectional survey was carried out among the 298 dentists of Aligarh and Gwalior. The questionnaire used in the study had sections on dentists' sociodemographic data and a 35-item questionnaire to assess the potential determinants of tobacco cessation counseling. Descriptive statistics were carried out, and a Chi-square test was utilized to determine the association. P-value <0.05 was considered statistically significant. Results: Domains "knowledge", "Professional Responsibility and Identity", and "Remembrance, awareness, and judgment" showed a statistically significant correlation with most tobacco cessation counseling behaviors. In addition, undergraduate education received in Tobacco Cessation counseling, and Continuing education received in Tobacco Cessation counseling had significantly impacted the practice of tobacco cessation counseling (p=0.02 and 0.04, respectively). Conclusion: This study suggests that "Knowledge", "Professional Responsibility and Identity" and "Remembrance, awareness, and judgment" are the potential determinants that could be used to design effective strategies to enhance tobacco counseling among dentists in India.
Gideon Obasanmi, Noemi Lois, David Armstrong
et al.
Inflammation is key to the pathogenesis of diabetic retinopathy (DR). This prospective study investigated alterations in inflammatory cytokines in peripheral blood mononuclear cells (PBMCs) in 41 people with type 1 diabetes (T1D), sub-grouped into mild non-proliferative DR (mNPDR; <i>n</i> = 13) and active and inactive (each <i>n</i> = 14) PDR. Age/gender-matched healthy controls (<i>n</i> = 13) were included. PBMCs were isolated from blood samples. Intracellular cytokine expression by PBMCs after 16-h stimulation (either <i>E. coli</i> lipopolysaccharide (LPS), phorbol 12-myristate 13-acetate plus ionomycin, D-glucose or D-mannitol) were assessed by flow cytometry. Cytokine production in plasma, non-stimulated and LPS-stimulated PBMC supernatant was also assessed. Increased BMC IL-10 secretion and reduced expression of IL-6 and IFN-γ in CD3<sup>+</sup> cells were observed in mNPDR. Reduced IL-6 and IL-10 secretion, and higher levels of intracellular IL-6 expression, especially in CD11b<sup>+</sup> PBMCs, was detected in aPDR; levels were positively correlated with DR duration. Patients with T1D demonstrated increased intracellular expression of IL-17A in myeloid cells and reduced IFN-γ expression in CD3<sup>+</sup> cells. Plasma levels of IL-1R1 were increased in mNPDR compared with controls. Results suggest that elevated PBMC-released IL-10, IL-6, in particular myeloid-produced IL-17A, may be involved in early stages of DR. IL-6-producing myeloid cells may play a role in PDR development.
Peri-implant diseases are prevalent conditions, but a predictable management strategy is still lacking. The objective of the present article was to evaluate the adjunctive benefits of probiotics with nonsurgical therapy in the management of peri-implant diseases. The review protocol was registered in PROSPERO and prepared according to PRISMA guidelines. Randomized controlled clinical trials in patients diagnosed with the peri-implant disease where probiotic was used as an adjunct to nonsurgical therapy were included in the study. The risk difference of percentage reduction in bleeding on probing, plaque accumulation, and mean difference in probing pocket depth reductions at implant level were estimated using a random effect model due to high heterogeneity among studies. Four studies fulfilled the criteria for selection. Two of them presented data on both peri-implantitis and peri-implant mucositis and they were considered separate studies during meta-analysis. Significant reduction in percentage of bleeding on probing was noticed at 1 and 3 months (−0.28 [−0.48, −0.09], P = 0.004 and − 0.19 [−0.35, −0.02], P = 0.03, respectively), but the reduction was not statistically significant at 6 months. Similar results were also observed for plaque accumulation. No statistically significant reduction in probing pocket depth was observed in the probiotic group during any of the re-evaluations. Conclusion: Adjunctive therapy of probiotics may improve the efficacy of nonsurgical therapy of peri-implant diseases for up to 3 months. However, moderate certainty was observed for a reduction in bleeding on probing after 1-month re-evaluation alone.
Takumi Takahashi, Miki Yamada, Keisuke Sawada
et al.
Hematopoietic stem cell transplantation (HSCT) is a common method for patients such as hematologic malignancies. However, HSCT generally has a higher risk of secondary solid cancer development. The aim of this study was to emphasize the need for lifelong follow-up of oral secondary solid cancer. The patient was a male who underwent HSCT for chronic myelogenous leukaemia at the age of 31 years. He underwent ten onsets on oral secondary solid cancers during his subsequent follow-up of more than 20 years. In conclusion, patients after HSCT require lifelong observation of oral secondary solid cancer, which may be accompanied by repeated new and recurrent occurrences.
The selection of restorative materials with regard to the longevity and durability of a restoration is of crucial importance for daily dental practice and requires that the degradation of the material in the oral environment can be assessed. The aim of this study was to investigate the extent to which the mechanical properties of four (Esthet X, Ceram X, Filtek Supreme XT, and Filtek Supreme XT flow) resin-based composites (RBCs) alter during storage in saliva substitutes (artificial saliva) for 24 h and 28 days and in the context of simulated, more aggressive clinical conditions, including cycles exposure to de- and remineralization, alcohol, or salivary enzymes. For this purpose, flexural strength and modulus were determined in a three-point bending test (<i>n</i> = 20) followed by Weibull analysis, while quasi-static behavior was evaluated by instrumented indentation techniques. Degradation occurred in all RBCs and all aging protocols and was quantifiable at both macroscopic and microscopic levels. The postulated stabilizing effect on degradation through the incorporation of urethane-based co-monomers into the organic matrix or a higher filler loading is refuted. Even though modern RBCs show high clinical survival rates, biodegradation remains an issue that needs to be addressed.
Malik Sallam, Kholoud Al-Mahzoum, Latefa Ali Dardas
et al.
<i>Background and Objective:</i> The recent multi-country outbreak of human monkeypox (HMPX) in non-endemic regions poses an emerging public health concern. University students in health schools/faculties represent a core knowledgeable group that can be helpful to study from a public health point of view. As future healthcare workers, assessment of their knowledge and attitude towards emerging zoonotic viral infections can be helpful to assess their taught material and courses with potential improvement if gaps in knowledge were identified. Therefore, we aimed to evaluate the level of HMPX knowledge, conspiracy beliefs regarding emerging virus infections, as well as their associated determinants among university students studying Medicine, Nursing, Dentistry, Pharmacy, Medical Laboratory Sciences, and Rehabilitation in Jordanian health schools/faculties. In addition, we sought to evaluate the correlation between HMPX knowledge and the extent of holding conspiracy beliefs regarding emerging viral infection. <i>Materials and Methods:</i> A convenient sample of university students was obtained through an electronic survey distributed in late May 2022 using the chain-referral approach. Assessment of HMPX knowledge and general attitude towards emerging virus infections was based on survey items adopted from previously published literature. <i>Results:</i> The study sample comprised 615 students with a mean age of 20 years and a majority of females (432, 70.2%) and medical students (<i>n</i> = 351, 57.1%). Out of eleven monkeypox knowledge items, three were identified correctly by >70% of the respondents. Only 26.2% of the respondents (<i>n</i> = 161) knew that vaccination to prevent monkeypox is available. Age was significantly associated with better HMPX knowledge for a majority of items. Older age, females, and affiliation to non-medical schools/faculties were associated with harboring higher levels of conspiracy beliefs regarding emerging virus infections. Our data also indicate that lower levels of HMPX knowledge were associated with higher levels of conspiracy beliefs. <i>Conclusion:</i> The current study pointed to generally unsatisfactory levels of knowledge regarding the emerging HMPX among university students in Jordanian health schools/faculties. Conspiracy beliefs regarding emerging virus infections were widely prevalent, and its potential detrimental impact on health behavior should be evaluated in future studies.
Diego Raimondo, Antonio Raffone, Antonio Raffone
et al.
Data about the oncological outcomes in women with borderline ovarian tumor (BOT) undergoing uterine-sparing surgery without ovarian preservation are poor. We aimed to assess the oncological outcomes in women with BOT undergoing uterine-sparing surgery without ovarian preservation. A multi-center observational retrospective cohort study was performed including all consecutive postmenopausal patients who underwent surgical treatment for BOT at three tertiary level referral centers for gynecologic oncology from January 2005 to December 2016. Patients were divided into two groups for comparisons: patients undergoing hysterectomy (hysterectomy group) and patients undergoing uterine-sparing surgery (no hysterectomy group). Study outcomes were disease-free survival (DFS), overall survival (OS), disease-specific survival (DSS) and surgical complications rate. Ninety-eight patients were included: 44 in the hysterectomy group and 54 in the no hysterectomy group. The 5- and 10-year DFS rates were 97.7% (95% CI: 84.9–99.7) and 92.3% (95% CI: 69.7–98.2), in the hysterectomy group, and 86.8% (95% CI: 74.3–93.5) and 86.8% (95% CI: 74.3–93.5), in the no hysterectomy group, respectively, without significant differences (p=0.16). Hazard ratio for DFS was 0.26 (95% CI: 0.06–1.68) for the hysterectomy group. The 5- and 10-year OS rates were 100.0% (95% CI: -) and 100.0% (95% CI: -), in the hysterectomy group, and 98.2% (95% CI: 87.6–99.7) and 94.4% (95% CI: 77.7–98.7), in the no hysterectomy group, respectively, without significant differences (p=0.23). No significant difference in complication rate was reported among the groups (p=0.48). As hysterectomy appears to not impact survival outcomes of women with BOT, it might be avoided in the surgical staging.
Neoplasms. Tumors. Oncology. Including cancer and carcinogens