Hasil untuk "Fermentation industries. Beverages. Alcohol"

Maider Lago, Ane Bordagaray, Ane Olañeta-Jainaga et al.

The cider industry generates substantial amounts of apple pomace (AP), a by-product rich in fermentable sugars, organic acids, and bioactive compounds. This study aimed to optimize the extraction of fermentable must from AP using a central composite design (CCD) and to evaluate its potential for producing non- or low-alcohol (NoLo) beverages through fermentation. The extraction process was optimized using a desirability function targeting maximum sugar (26.69 g/L), malic acid (1.30 g/L), and nitrogen content (29.60 mg/L). The model revealed that extraction time and agitation had significant effects on sugar and malic acid, while an enzyme was less influential. The selected optimal condition was 4.6 h of maceration without enzyme addition providing an efficient must composition. Must was then fermented using combinations of <i>Saccharomyces cerevisiae</i>, <i>Lactobacillus plantarum</i>, and <i>Pichia kluyveri</i>. Physicochemical, microbiological, and volatile compound analyses were conducted throughout fermentation. Results showed that fermentation time and microbial composition significantly influenced sugar degradation, ethanol and lactic acid production and malic acid conversion, while mixed fermentation <i>S. cerevisiae</i> + <i>L. plantarum</i> and <i>L. plantarum</i> + <i>P. kluyveri</i> demonstrated the most promising aromatic profiles. Principal component analysis (PCA) confirmed distinct fermentation trajectories based on microbial consortia, highlighting the potential of AP as a sustainable substrate for developing innovative NoLo beverages.

Nida Arshad, Elizabeth Jayex Panakkal, Palani Bharathy Kalivarathan et al.

The global reliance on fossil fuels has caused severe environmental challenges, emphasizing the urgent need for sustainable and renewable energy sources. Bioethanol production from lignocellulosic biomass has emerged as a promising alternative due to its abundance, renewability, and carbon-neutral footprint. However, its economic feasibility remains a major obstacle owing to high production costs, particularly those associated with low ethanol titers and the energy-intensive distillation process costs for low titers. High-solid loading processes (≥15% <i>w</i>/<i>w</i> or <i>w</i>/<i>v</i>) have demonstrated potential to overcome these limitations by minimizing water and solvent consumption, enhancing sugar concentrations, increasing ethanol titers, and lowering downstream processing cost. Nevertheless, high-solid loading also introduces operational bottlenecks, such as elevated viscosity, poor mixing, and limited mass and heat transfer, which hinder enzymatic hydrolysis efficiency. This review critically examines emerging pretreatment and enzymatic hydrolysis strategies tailored for high-solid loading conditions. It also explores techniques that improve sugar yields and conversion efficiency while addressing key technical barriers, including enzyme engineering, process integration, and optimization. By evaluating these challenges and potential mitigation strategies, this review provides actionable insights to intensify lignocellulosic ethanol production and advance the development of scalable, cost-effective biorefinery platforms.

Wengboyang Liu, Zhiqiang Cheng, Yujie Zong et al.

Rumen bacteria have the ability to efficiently degrade and acidify lignocellulosic biomass, among which rumen solid-phase bacteria are more dominant. However, the effectiveness of in vitro cultured ruminal solid-phase bacteria in producing volatile fatty acids (VFA) during lignocellulosic biomass degradation remains unclear. This study presents a feasibility analysis of the long-term subculture of rumen solid-phase bacteria in vitro for VFA production. The results indicated that VFA production could reach 0.20–0.30 g/g dry matter. After 40 generations (200 days) of subculturing, the bacterial community underwent alterations. The relative abundance of certain fiber-degrading, acid-producing bacteria, which were less abundant in rumen solids, such as <i>Oribacterium</i> and <i>Victivallis</i>, was significantly upregulated following subculturing in vitro. The success of this study in subculturing rumen solid-phase bacteria in vitro over an extended period and achieving efficient VFA production is of considerable importance for the practical application of rumen microorganisms in production settings.

Muhammad Sohail

Biorefineries integrate various processes to obtain different products from a single waste material [...]

Tania Picado-Pérez, Rocky Lemus, Daniel Rivera et al.

Climate shifts have significantly affected livestock systems due to their environmental interdependence. Among the strategies adopted by livestock systems to fill the gaps in forage biomass, preserved forages are the most commonly used. This research assessed the nutritional profile of African Stargrass (<i>Cynodon nlemfuensis</i> Vanderyst) preserved as silage and haylage at different feed-out periods (45, 60, 90, and 120 days). We found greater dry matter (DM) content in haylage (29.7%), with no important variations in silage over time. Stargrass silage had crude protein (CP) levels greater (13.0%) than haylage (11.9%); the former was not affected by the duration of the preservation period. Silage had lower levels of neutral detergent fiber (NDF) and higher levels of in vitro dry matter digestibility (IVDMD). The net energy for lactation (NEL) was similar for the two types of preservations evaluated. A principal component analysis (PCA) revealed that most of the variance in the dataset (69.6%) was explained by two principal components. PC1 showed that the most relevant variables were ADF, α-NDF, dNDF30, d NDF48, and NDICP, while Ash, Ca, and insoluble CP were the most relevant variables in PC2. Unlike haylage, the nutritional value of silage remained constant (<i>p</i> > 0.05) up to 120 days of preservation. It is important to note that haylage should not be stored beyond 90 days, regardless of the type of preservation.

He Meng, Yan Jiang, Lin Wang et al.

Soybean (<i>Glycine max</i> (L.) Merr.) is an important oilseed crop, known for its rich nutritional content and high-quality protein. To address the shortage of feed protein resources and better utilize soybeans as a raw material, this study investigated the feasibility of using whole-plant soybean (WPS) as silage. As the ensiling period is a critical fermentation parameter, identifying the optimal fermentation duration was a key objective. The research involves fermenting WPS for silage production, conducted over five fermentation durations: 7, 15, 30, 60, and 90 days. The fermentation quality, microbial community, and metabolome of WPS silage were analyzed across these different time points. WPS silage fermented for 30 days exhibited optimal fermentation characteristics, with the highest lactic acid (LA) content observed at 30 days (<i>p</i> < 0.05), while butyric acid (BA) was detected only at 60 and 90 days. At 30 days, <i>Enterococcus</i> genera reached its peak relative abundance and was identified as the dominant genus. Random forest analysis highlighted <i>Pantoea</i> genera as the most influential biomarker. Metabolomic analysis revealed that the metabolic pathways involved in the biosynthesis of essential amino acids valine, leucine, and isoleucine were significantly enhanced during the later stages of fermentation compared to the earlier stages. Under natural fermentation conditions, the optimal fermentation period for WPS silage is approximately 30 days. These findings provide a theoretical basis for the utilization of WPS and the subsequent optimization of fermentation quality.

Dornoush Jafarpour, Seyed Mohammad Bagher Hashemi

In this study, <i>Limosilactobacillus fermentum</i> PTCC 1638 and <i>Lacticaseibacillus rhamnosus</i> PTCC 1637 were used alone and in combination to ferment quinoa seeds, and the effect of fermentation (37 °C; 24 h) on the pH, total phenols, tocopherols, vitamin C, antioxidant activity, and enzymes inhibition (α-amylase and α-glucosidase; antidiabetic effect) was investigated. The results showed that with the increase in the fermentation time, the bacterial population, total phenols, antioxidant activity, and enzymes inhibition increased, which showed the greatest increase for the co-culture of <i>L. rhamnosus</i> and <i>L. fermentum</i> compared to the pure culture of each strain. Due to the increase in the fermentation time, the tocopherol isomers (α, β, γ, and δ), vitamin C, and pH decreased, and the largest decrease was related to the co-culture of the strains, followed by <i>L. rhamnosus</i> and <i>L. fermentum</i>. The results of this study showed that the co-culture and pure culture of bacteria can have different effects on the physicochemical properties and bioactive compounds of quinoa seeds.

Vikas Sharma, Abul Kalam Hossain, Ganesh Duraisamy et al.

By 2050, aviation-related carbon emissions are expected to quadruple to over 3000 million tonnes of carbon dioxide, so finding sustainable alternative solutions to minimise pollution is a key scientific challenge. Aviation gasoline and kerosene are currently used to power most jet engines. While battery-powered planes and planes that could utilise a cleaner fuel, such as hydrogen, are possible, the time scale required to improve and implement these technologies is distant, with air fleet turnover taking some 30 years. Existing jet engines could be modified to run on biodiesel, and considering the close similarity in fuel density to kerosene, could be a less disruptive approach to the industry. The sheer volume of biodiesel required remains a challenge, and certainly, using plant-derived oils grown on arable land is not acceptable, as it competes with food production. However, high-lipid-yielding microalgae (where productivity is an order of magnitude greater than oilseeds), grown on marginal land, such as desert or semi-desert areas of the world, could be possible. Indeed, to replace 30% of fossil fuel with algal-derived biodiesel would require 11,345 km² of land. Biodiesel preparation is well understood, but what is lacking is proven technology aimed at optimising microalgal production of oil at a much larger scale. Here, a synergic review of the current state-of-the-art in algal production, that includes strain selection, possible production sites, culturing costs, and harvesting to identify the bottlenecks in meeting the ASTM specifications for the aviation industry, is presented.

Jennifer R. Weinert-Nelson, Donald G. Ely, Michael D. Flythe et al.

Sheep are often utilized as a model ruminant, despite a lack of functional comparisons of rumen bacterial communities and responses during dietary transitions between sheep and cattle. Therefore, an <i>ex vivo</i> study was conducted to evaluate species differences. Rumen fluid was obtained from hay-fed sheep and cattle (<i>n</i> = 3 species⁻¹). Mixed bacterial cell suspensions in buffered media containing 3% <i>w</i>/<i>v</i> ground hay, corn, or combinations (2:1, 1:2) of substrates were incubated (24 h; 39 °C). Suspension pH, lactate, volatile fatty acids (VFA), and digestibility were assessed, functional guilds enumerated, and amylolytic bacteria isolated. Lactate was fully utilized in all hay incubations, and pH did not differ between species (<i>p</i> > 0.75). In contrast, digestibility, lactate accumulation, and pH decline were greater in bovine suspensions fermenting corn (<i>p</i> < 0.01). <i>Streptococcus bovis</i> was the predominant bacteria regardless of species, but total amylolytic bacteria were 10-fold greater in bovine suspensions (<i>p</i> < 0.01). Lactate-utilizing bacteria were 1000-fold greater in bovine than ovine suspensions (<i>p</i> < 0.01). However, total VFA did not differ between species (<i>p</i> > 0.28). Overall, these results demonstrate differential feed utilization capacities in rumen microbial communities of sheep and cattle as well as potential differences in rumen acidosis susceptibility.

Diana Laura Quezada-Morales, Juan Campos-Guillén, Francisco Javier De Moure-Flores et al.

Lignocellulosic biomass is a source of carbohydrates that can be used in the production of biogas. The aim of this study was to obtain biogas from biomass waste (leaves, stems and seed bagasse) of <i>Ricinus communis</i>, applying pretreatments such as temperature and humidity. We examined the effect of these pretreatments on the biomass, two enzymatic pretreatments (cellulase and cellobiohydrolase), two chemicals (NaOH and HCl) and two controls (dried castor straw and seed bagasse) on the methane content. The experiment was performed in two anaerobic digestion (AD) assays at a controlled temperature (37 °C) and at room temperature, with a hydraulic retention time (HRT) of 55 days. The results showed that the residues of the seed bagasse produced the highest biogas yields both at room temperature and at the controlled temperature since this material at 37 °C produced 460.63 mL gVS⁻¹ under cellulase pretreatment; at room temperature, the highest level of production was found for the control (263.41 mL gVS⁻¹). The lowest yields at the controlled temperature and room temperature were obtained from residues of <i>Ricinus communis</i> treated with cellobiohydrolase and the seed bagasse treated with alkaline (15.15 mL gVS⁻¹ and 78.51 mL gVS⁻¹, respectively). Meanwhile, the greatest amount of methane was produced by seed bagasse treated with cellobiohydrolase at a controlled temperature (92.2% CH₄) and the lowest content of CH₄ (15.5%) was obtained at a controlled temperature from castor straw under the control treatment.

Łukasz Łopusiewcz, Natalia Śmietana, Elżbieta Lichwiarska et al.

In recent years, there has been increasing consumer interest and research into plant-based dairy alternatives, due to the increasingly negative impact of animal products on human health, animal welfare, and the environment. The purpose of this study was to investigate the physicochemical and microbiological changes in a Camembert alternative based on the seeds of sweet lupine (<i>Lupinus angustifolius</i> L cv. ‘Boregine’). After heat treatment and homogenization, the seeds were incubated with lactic acid bacteria (LAB) and <i>Penicillium camemberti</i> mold. After fermentation at room temperature, the samples were stored at 12 °C for 14 days, and then ripened until day 49 at 6 °C. Changes in microbial population, acidity, texture, content of polyphenols, flavonoids, reducing sugars, and free amino acids were monitored. In addition, the antioxidant capacity of the samples during ripening was determined. The results showed that LAB and fungi were able to grow well in the lupine matrix. Initially, a decrease in pH was observed, while in the further stages of ripening, alkalization of the product linked with progressive proteolysis associated with an increase in free amino acid content was noted. Hydrolysis of polysaccharides and an increase in antioxidant activity were observed. This indicates the potential of lupine seeds as a raw material for the development of a new group of plant-based ripened cheese alternatives.

Natthiporn Aramrueang, Passanun Lomwongsopon, Sasiprapa Boonsong et al.

Volatile fatty acids (VFAs) are the important intermediates indicating the stability and performance of fermentation process. This study developed the spectrophotometric method for determining high-range VFA concentration in mixed-acid fermentation samples. The performance was compared with the gas chromatography (GC) technique. The calibration curves of the modified method showed linearity over a wide and high concentration range of 250–5000 mg/L for individual C2–C6 VFAs in both linear and branched chains. In order to evaluate the modified method for VFA determination in complex fermentation matrices, fermentation samples produced from acidogenic fermentation of plant materials were spiked with acetic (500–1500 mg/L) and butyric acids (1000 mg/L). The accuracy and precision of the modified method for VFA determination were in the range of 94.68–106.50% and 2.35–9.26%, respectively, comparable to the GC method (94.42–99.13% and 0.17–1.93%). The developed method was applicable to measuring all C2–C6 compounds and VFA concentrations in the fermentation samples and had an acceptable accuracy and precision. The proposed method is analytically reliable and offers significant advantages in the rapid determination of VFAs in mixed acid fermentation of organic residues.

Getari Kasmiarti, Dwita Oktiarni, Poedji Loekitowati Hariani et al.

Yeast is a fermentation agent for producing bioethanol as an environmentally friendly alternative energy. Therefore, this study aims to find novel yeasts with the capability to persevere under acidic, high temperature, and high sugar content conditions, which are required in the bioethanol industry. The yeasts were isolated and identified from coconut (<i>Cocos nucifera</i> L.) water by a DNA sequencing method and phenotypic test. Yeast isolation has been completed with a serial dilution procedure and purification was conducted with HiPurA Genomic DNA Purification Spin Kits, which were analyzed by DNA Sequencing. The phenotypic test was carried out with thermotolerant (30 °C and 41 °C), high acidity (lactic acid), and sugar content (molasses 35 °brix) parameters in the media as the initial step of yeast ability screening. Based on the results, the three species of <i>Candida tropicalis</i> K5 (<i>Candida tropicalis</i> strain L2), K15 (<i>Candida tropicalis</i> strain MYA-3404), and K20 (<i>Candida tropicalis</i> strain Y277) obtained met the phenotypic standards. This showed that the yeasts have the potential to produce molasses-based bioethanol.

Ming-Chih Fang, Irene Han-Juo Cheng, Chien-Li Chen

Evidence suggests that various hallmarks such as amyloid overproduction, tau dysfunction, insulin resistance/diabetic mechanisms, and neuroinflammation are associated with Alzheimer’s disease (AD). This study investigated the bioactive functions of ankaflavin (AK) and monascin (MS) in the fermented product of <i>Monascus purpureus</i> and found their abilities to ameliorate AD by modifying several important pathogenic factors including improved cognitive function, reversed behavioral deficits, reduced hippocampal β-amyloid peptide (Aβ) burden, decreased tau hyper-phosphorylation, and reduced neuroinflammation in the J20 mouse model of AD compared to wild type. <i>Monascus purpureus</i> fermented product (MPFP) was suggested to act as a peroxisome proliferator-activated receptor (PPAR)-γ agonist and it was compared against the action of a well-known anti-diabetic PPAR-γ agonist rosiglitazone. MPFP could be a promising therapeutic strategy for disease modification in AD.

Cheng Guo, Yaqi Wu, Shengli Li et al.

Forages are vital in maintaining the dietary structures of ruminants, and reducing their costs is important for improving dairy production efficiency. Thus, this study investigated the effects of dietary forage types on dry matter intake, production, rumen fermentation, and the microbial profile in peak-lactating cows. Eight cows (600 ± 25 kg) with days in milk (60 ± 10 days) were assigned to four groups using a replicated 4 × 4 Latin square design: OG (oat hay + alfalfa hay + corn silage + concentrate), CW (<i>Leymus chinensis</i> + alfalfa hay + corn silage + concentrate), AS (alfalfa silage + oat hay + corn silage + concentrate), and AC (alkali-treated corn straw + alfalfa hay + corn silage + concentrate). The ruminal butyrate acid concentration was lower in the OG group than in the AS and AC groups post-feeding (12 h; <i>p</i> < 0.05). Ruminal NH3-N content was higher in the AS group than in the AC and CW groups post-feeding (9 h; <i>p</i> < 0.05). The percentage of ruminal <i>Oscillospira</i> and unknown microbes was higher in the CW group than in the other groups (<i>p</i> < 0.05). The total rumination time and rumination time per dry matter intake of AC were significantly higher than those of the other groups (<i>p</i> < 0.05). Milk lactose content in the AS group was highest among the groups (<i>p</i> < 0.05), and milk fat content was higher in the OG group than in the CW group (all <i>p</i> < 0.05). Overall, our results suggested that the butyrate acid content of forage from oat hay in rumen fermentation was significantly lower than that of calcium oxide and alfalfa silage sources when the dietary nutrition level was similar. The diet derived from <i>L. chinensis</i> increased the number of fibrillation helices related to fiber-decomposing bacteria and simultaneously increased unknown strains. Forage derived from alfalfa silage tended to increase milk protein levels. Alkali-treated corn straw could significantly increase the total rumination time and unit dry matter rumination time of dairy cows, which plays an important role in maintaining rumen health. The rational use of low-quality forage has broad prospects in China.

Catarina M. de Figueiredo, Daniella H. Hock, Débora Trichez et al.

Many contaminant yeast strains that survive inside fuel ethanol industrial vats show detrimental cell surface phenotypes. These harmful effects may include filamentation, invasive growth, flocculation, biofilm formation, and excessive foam production. Previous studies have linked some of these phenotypes to the expression of <i>FLO</i> genes, and the presence of gene length polymorphisms causing the expansion of <i>FLO</i> gene size appears to result in stronger flocculation and biofilm formation phenotypes. We performed here a molecular analysis of <i>FLO1</i> and <i>FLO11</i> gene polymorphisms present in contaminant strains of <i>Saccharomyces</i> <i>cerevisiae</i> from Brazilian fuel ethanol distilleries showing vigorous foaming phenotypes during fermentation. The size variability of these genes was correlated with cellular hydrophobicity, flocculation, and highly foaming phenotypes in these yeast strains. Our results also showed that deleting the primary activator of <i>FLO</i> genes (the <i>FLO8</i> gene) from the genome of a contaminant and highly foaming industrial strain avoids complex foam formation, flocculation, invasive growth, and biofilm production by the engineered (<i>flo</i>8∆::<i>Ble</i>^R/<i>flo8</i>Δ::<i>kanMX</i>) yeast strain. Thus, the characterization of highly foaming yeasts and the influence of <i>FLO8</i> in this phenotype open new perspectives for yeast strain engineering and optimization in the sugarcane fuel-ethanol industry.

Janis Liepins, Karina Balina, Raimonda Soloha et al.

Biosurfactants are a microbially synthesized alternative to synthetic surfactants, one of the most important bulk chemicals. Some yeast species are proven to be exceptional biosurfactant producers, while others are emerging producers. A set of factors affects the type, amount, and properties of the biosurfactant produced, as well as the environmental impact and costs of biosurfactant’s production. Exploring waste cooking oil as a substrate for biosurfactants’ production serves as an effective cost-cutting strategy, yet it has some limitations. This review explores the existing knowledge on utilizing waste cooking oil as a feedstock to produce glycolipid biosurfactants by yeast. The review focuses specifically on the differences created by using raw cooking oil or waste cooking oil as the substrate on the ability of various yeast species to synthesize sophorolipids, rhamnolipids, mannosylerythritol lipids, and other glycolipids and the substrate’s impact on the composition, properties, and limitations in the application of biosurfactants.

Kateřina VACULOVÁ, Marta BALOUNOVÁ, František KVASNIČKA et al.

Around 65 % of phosphorus (P) in barley grain is in the form of phytic acid (PA) and phytates (PA salts), both forms negatively affect the usability of mineral substances and other important nutrients, thus participating in mineral deficiencies in human as well as livestock nutrition, and contributing to the pollution of the environment. Detection and creation of new varieties with a changed rate of both P forms in favor of digestible phosphate (Pi) is one of possible solutions. The submitted study dealt with the evaluation of variability in contents of PA and Pi in grains of selected spring and winter barley varieties, genetic resources and new spring barley lines created by hybridization with so-called "lpa" chemo-mutants - donors of low PA content (M422, M1070, M635, and M955). Variability in the content of both P forms in spring barley was affected by year and only if the assessed set included lpa mutants also by a genotype. In spite of high year variability, Pi/ PA rate in lpa mutants remained nearly the same (from 138.4 % in M422 to 772.1 % in M955), while in the standard varieties it was on average only 31.3 %. The comparison of winter and spring barley varieties proved that winter varieties have a strong tendency to a higher accumulation of P in grain in the PA form (12.42 mg.g-1 vs. 10.94 mg.g-1). The screening method developed for the evaluation of free P content in grain using the colorimetric test (CT) allowed to perform a more extensive selection of materials with different PA and Pi contents. A strong positive correlation was calculated between the results obtained by the CT and classically determined Pi (r = 0.85**, P≤0.01). On the other hand, the use of existing molecular markers (SCAR marker ABC153) for the detection of crosses with reduced PA content in grain was not very effective. Crossing with lpa mutants and following selection using the CT led to creation of new lines of spring barley with reduced PA content, increased Pi rate and comparable total P content in a caryopsis, which can be used in breeding or further research. A new line KM2881.622.2.07 where an increased content of free P is connected with a waxy starch character (reduced portion of polysaccharide amylose) can be a new interesting gene resource for the development of food barley varieties.Around 65 % of phosphorus (P) in barley grain is in the form of phytic acid (PA) and phytates (PA salts), both forms negatively affect the usability of mineral substances and other important nutrients, thus participating in mineral deficiencies in human as well as livestock nutrition, and contributing to the pollution of the environment. Detection and creation of new varieties with a changed rate of both P forms in favor of digestible phosphate (Pi) is one of possible solutions. The submitted study dealt with the evaluation of variability in contents of PA and Pi in grains of selected spring and winter barley varieties, genetic resources and new spring barley lines created by hybridization with so-called "lpa" chemo-mutants - donors of low PA content (M422, M1070, M635, and M955). Variability in the content of both P forms in spring barley was affected by year and only if the assessed set included lpa mutants also by a genotype. In spite of high year variability, Pi/ PA rate in lpa mutants remain

Jiří ČULÍK, Tomáš HORÁK, Marie JURKOVÁ et al.

Thermogravimetric determination of the moisture content in native samples with the aid of HR 83 (Mettler Toledo) analyser is based on the concept of halogen heating and an automated monitoring of the course of the kilning curve. The possibility of utilizing this instrument in the field was tested on real samples of barley, green malt and malt. Validation of the methods for determining dry matter in barley, green and finished malt was performed. Based on the obtained observations, it can be stated that this instrument can be recommended to malting and brewing laboratories.Thermogravimetric determination of the moisture content in native samples with the aid of HR 83 (Mettler Toledo) analyser is based on the concept of halogen heating and an automated monitoring of the course of the kilning curve. The possibility of utilizing this instrument in the field was tested on real samples of barley, green malt and malt. Validation of the methods for determining dry matter in barley, green and finished malt was performed. Based on the obtained observations, it can be stated that this instrument can be recommended to malting and brewing laboratories.

Richard POSPIŠIL

Field trial in which we observed the influence of decayed waste on spring barley yield and quality was founded University farming business in Kolíňany in 2004-2005. The highest average grain yield and biological yield (phytomass dry mater) were reached with spring decayed waste application (6,14 t.ha-1, resp. 12,56 t.ha-1). The lowest values of gross protein provided "Manure 25 t.ha-1" variant (9,0%) in average of 2004-2005 and in 2005 variant with application of 50 t.ha-1 of decayed waste (10,4%).Field trial in which we observed the influence of decayed waste on spring barley yield and quality was founded University farming business in Kolíňany in 2004-2005. The highest average grain yield and biological yield (phytomass dry mater) were reached with spring decayed waste application (6,14 t.ha-1, resp. 12,56 t.ha-1). The lowest values of gross protein provided "Manure 25 t.ha-1" variant (9,0%) in average of 2004-2005 and in 2005 variant with application of 50 t.ha-1 of decayed waste (10,4%).