Ruminant animals depend on cellulolytic ruminal bacteria to digest cellulose, but these bacteria cannot resist the low ruminal pH that modern feeding practices can create. Because the cellulolytic bacteria cannot grow on cellobiose at low pH, pH sensitivity is a general aspect of growth and not just a limitation of the cellulases per se. Acid-resistant ruminal bacteria have evolved the capacity to let their intracellular pH decrease, maintain a small pH gradient across the cell membrane, and prevent an intracellular accumulation of VFA anions. Cellulolytic bacteria cannot grow with a low intracellular pH, and an increase in pH gradient leads to anion toxicity. Prevotella ruminicola cannot digest native cellulose, but it grows at low pH and degrades the cellulose derivative, carboxymethylcellulose. The Prevotella ruminicola carboxymethylcellulase cannot bind to cellulose, but a recombinant enzyme having the Prevotella ruminicola catalytic domain and a binding domain from Thermomonspora fusca was able to bind and had cellulase activity that was at least 10-fold higher. Based on these results, gene reconstruction offers a means of converting Prevotella ruminicola into a ruminal bacterium that can digest cellulose at low pH.
This analysis aims to adequately explain the atomic nucleus. Scientists are stuck to model proposed in 1950 according to which protons move along orbitals, but the measured radius of nucleus is such that only three protons can fit inside it, and certainly they do not orbit. Therefore, the model valid for electrons, which travel through enormous spaces, is not suitable for nucleus. Nucleus at Polyhedra Shells is a compact structure where protons are held by neutrons, thanks to the force of gluons, but repulsion distributes them equidistantly. This structure grows and reshapes with atomic number (Z), from hydrogen to last element, changing properties of elements and isotopes between instability and stability, until those who take on a magic number 8, 20, 28, 50, and 82; these properties are reported in tables and drawings to facilitate comparison. The result is a nucleus as an aggregate of protons and neutrons in approximately equal numbers, compact but with a fluidity that pushes the positive charges of protons to stay as far apart as possible but held by the neutrons that act as dielectric and glue. The balance of repulsive and union forces leads to an architecture of concentric shells, each in shape of a crystal whose vertices are occupied by protons at maximum distance from each other, and faces by neutrons, in a balanced geometric configuration that allows the stability and existence of nucleus, and chemical elements.
Yuri B. Ovchinnikov, Folly Eli Ayi-Yovo, Alessio Spampinato
Optical dipole micro-traps for atoms based on constructive superposition of two-colour evanescent light waves, formed by corresponding optical modes of two crossed suspended photonic rib waveguides, are modelled. The main parameters of the traps for rubidium atoms, such as potential depth, tunnelling rates of atoms from the trap and coherence time of the trapped atoms are estimated. Applications of such traps for quantum memory and quantum logic devices are discussed.
The biochemical, biophysical, and physiological properties of the PsbS protein were studied in relation to mutations of two symmetry-related, lumen-exposed glutamate residues, Glu-122 and Glu-226. These two glutamates are targets for protonation during lumen acidification in excess light. Mutation of PsbS did not affect xanthophyll cycle pigment conversion or pool size. Plants containing PsbS mutations of both glutamates did not have any rapidly inducible nonphotochemical quenching (qE) and had similar chlorophyll fluorescence lifetime components as npq4-1, a psbS deletion mutant. The double mutant also lacked a characteristic leaf absorbance change at 535 nm (ΔA535), and PsbS from these plants did not bind dicyclohexylcarbodiimide (DCCD), a known inhibitor of qE. Mutation of only one of the glutamates had intermediate effects on qE, chlorophyll fluorescence lifetime component amplitudes, DCCD binding, and ΔA535. Little if any differences were observed comparing the two single mutants, suggesting that the glutamates are chemically and functionally equivalent. Based on these results a bifacial model for the functional interaction of PsbS with photosystem II is proposed. Furthermore, based on the extent of qE inhibition in the mutants, photochemical and nonphotochemical quenching processes of photosystem II were associated with distinct chlorophyll fluorescence life-time distribution components.
This paper examines a way to simplify the circuit of quantum random walk search algorithm, when the traversing coin is constructed by both generalized Householder reflection and an additional phase multiplier. If an appropriate relation between corresponding parameters is realized, our algorithm becomes more robust to deviations in the phases. In this modification marking coin is not needed, and all advantages from above mentioned optimization to the stability, are preserved. It is shown explicitly how to construct such walk coin in order to obtain more robust quantum algorithm.
We investigate the thermodynamic limit of Dicke superradiance. We find an expression for the system's density matrix that we can prove is exact in the limit of large atom numbers N. This is in contrast to previously known solutions whose accuracy has only been established numerically and that are valid only for a range of times. We also introduce an asymptotically exact solution when the system is subject to additional incoherent decay of excitations as this is a common occurrence in experiment.
Membrane and nuclear proteins of poor solubility have been separated by high resolution two‐dimensional (2‐D) gel electrophoresis. Isoelectric focusing with immobilized pH gradients leads to severe quantitative losses of proteins in the resulting 2‐D map, although the resolution is usually high. Protein solubility could be improved by using denaturing solutions containing various detergents and chaotropes. Best results were obtained with a denaturing solution containing urea, thiourea, and detergents (both nonionic and zwitterionic). The usefulness of thiourea‐containing denaturing mixtures is shown for microsomal and nuclear proteins as well as for tubulin, a protein highly prone to aggregation.
Kingshuk Adhikary, Subhanka Mal, Abhik Kr. Saha
et al.
In early 90's Mandel and coworkers performed an experiment \cite{mandel} to examine the significance of quantum phase operators by measuring the phase between two optical fields. We show that this type of quantum mechanical phase measurement is possible for matter-waves of ultracold atoms in a double well. In the limit of low number of atoms quantum and classical phases are drastically different. However, in the large particle number limit, they are quite similar. We assert that the matter-wave counterpart of the experiment \cite{mandel} is realizable with the evolving technology of atom optics.
In a recent work, we provided a standardized and exact analytical formalism for computing in the semiclassical regime the radiation force experienced by a two-level atom interacting with any number of plane waves with arbitrary intensities, frequencies, phases, and propagation directions [J. Opt. Soc. Am. B \textbf{35}, 127-132 (2018)]. Here, we extend this treatment to the multilevel atom case, where degeneracy of the atomic levels is considered and polarization of light enters into play. A matrix formalism is developed to this aim.
In this article, we propose a method to realize the "delayed choice experiment" using ultra-cold atoms. Here we attempt to probe the "welcher-Weg" information without collapsing the wavefunction of the atom. This experiment consists of components built around proven techniques that are put together in novel configuration to preserve the coherence of the system during the measurement. The Ramsey interference is used to establish the wave nature of the atom and the particle nature of the atom is probed by detecting its internal state by performing a nondemolition measurement using an ultra-high finesse cavity. The coherence of the atom is preserved by adjusting the atom-cavity interaction time such that the state of the atom is unchanged when it emerges out of the cavity.
We propose and demonstrate phonon-number-resolving detection of the multiple local phonon modes in a trapped-ion chain. To mitigate the effect of phonon hopping during the detection process, the probability amplitude of each local phonon mode is mapped to the auxiliary long-lived motional ground states. Sequential state-dependent fluorescence detection is then performed. In the experiment, we have successfully observed the time evolution of two local phonon modes in two ions, including the phonon-number correlation between the two modes.