Stephan Berner, A. Schmidt, Frowin Ellermann
et al.
The signal enhancement provided by the hyperpolarization of nuclear spins of metabolites is a promising technique for diagnostic magnetic resonance imaging (MRI). To date, most 13C-contrast agents are hyperpolarized utilizing a complex or cost-intensive polarizer. Recently, the in situ parahydrogen-induced 13C hyperpolarization was demonstrated. Hydrogenation, spin order transfer (SOT) by a pulsed NMR sequence, in vivo administration, and detection was achieved within the magnet bore of a 7 Tesla MRI system. So far, the hyperpolarization of the xenobiotic molecule 1-13C-hydroxyethylpropionate (HEP) and the biomolecule 1-13C-succinate (SUC) through the PH-INEPT+ sequence and a SOT scheme proposed by Goldman et al., respectively, was shown. Here, we investigate further the hyperpolarization of SUC at 7 Tesla and study the performance of two additional SOT sequences. Moreover, we present first results of the hyperpolarization at high magnetic field of 1-13C-phospholactate (PLAC), a derivate to obtain the metabolite lactate, employing the PH-INEPT+ sequence. For SUC and PLAC, 13C polarizations of about 1-2% were achieved within seconds and with minimal equipment. Effects that potentially may explain loss of 13C polarization have been identified, i.e. low hydrogenation yield, fast T1/T2 relaxation and the rarely considered 13C isotope labeling effect.
Heparin was immobilized on magnetic chitosan particles to be used as a tool for human plasma protein identification. Chitosan was magnetized by co-precipitation with Fe2+/Fe3+ (MAG-CH). Heparin was functionalized with carbodiimide and N-hydroxysuccinimide and covalently linked to MAG-CH (MAG-CH-hep). X-ray diffraction confirmed the presence of chitosan and Fe3O4 in MAG-CH. This particle exhibited superparamagnetism and size between 100-300 μm. Human plasma diluted with 10 mM phosphate buffer (pH 5.5) or 50 mM Tris-HCl buffer (pH 8.5) was incubated with MAG-CH-hep, and the proteins fixed were eluted with the same buffers containing increasing concentrations of NaCl. The proteins obtained were investigated by SDS-PAGE, LC/MS, and biological activity tests (PT, aPTT, and enzymatic chromogenic assay). Inhibitors of the serpin family, prothrombin, and human albumin were identified in this study. Therefore, MAG-CH-hep can be used to purify these proteins and presents the following advantages: low-cost synthesis, magnetic separation, ion-exchange purification, and reusability.
A simple model for QCD dynamics in which the DGLAP integro-differential equation may be solved analytically has been considered in our previous papers arXiv:1611.08787 [hep-ph] and arXiv:1906.07924 [hep-ph]. When such a model contains only one term in the splitting function of the dominant parton distribution, then Bessel function appears to be the solution to this simplified DGLAP equation. To our knowledge, this model with only one term in the splitting function for the first time has been proposed by Blümlein in arXiv:hep-ph/9506403. In arXiv:1906.07924 [hep-ph] we have shown that a dual integro-differential equation obtained from the DGLAP equation by a complex map in the plane of the Mellin moment in this model may be considered as the BFKL equation. Then, in arXiv:1906.07924 we have applied a complex diffeomorphism to obtain a standard integral from Gradshteyn and Ryzhik tables starting from the contour integral for parton distribution functions that is usually taken by calculus of residues. This standard integral from these tables appears to be the Laplace transformation of Jacobian for this complex diffeomorphism. Here we write up all the formulae behind this trick in detail and find out certain important points for further development of this strategy. We verify that the inverse Laplace transformation of the Laplace image of the Bessel function may be represented in a form of Barnes contour integral.
Abstract In this work, a study of Lecithin–heparin intercalated in montmorillonite was performed in order to synthesize an antithrombogenic hybrid. The hybrid of montmorillonite (Mt)/Lecithin–heparin (LEC-HEP) was synthesized by the intercalation method under mechanical stirring. The composite was characterized by X-ray diffraction (XRD) analysis, X-ray fluorescence (XRF) and C, H, and N elemental analyses, zeta potentials, Fourier transform infrared spectroscopy (FTIR) and thermogravimetric analysis (TGA) techniques. The d001-value was increased by LEC-HEP intercalation. Fourier transform infrared spectroscopy also confirmed the intercalation of Lecithin–heparin into Mt interlayer space. In vitro release study of the antithrombogenic drug–Mt intercalates in phosphate buffer saline (pH 7.4) media at 37 °C was investigated. The heparin showed an initial burst effect for 24 h and then continuously released for 30 d. In-vitro blood compatibility of the Mt/LEC-HEP was carried out via hemolysis assay. The Mt/LEC-HEP exhibited hemolysis below 5% which is permissible for biomaterials. These modified materials have the potential for being used as blood contact materials.