Hasil untuk "physics.app-ph"

J. W. Clayton, D. Tretiak

R. B. Moon, J. Richards

Observation of the ^(31)P signal from various intracellular phosphates can provide a convenient, nondestructive technique for determining intracellular conditions such as pH. This procedure has been explored with particular reference to the erythrocyte. Both the chemical shift of intracellular inorganic phosphate relative to that of serum phosphate and the positions of, and more especially the difference between, the chemical shifts of 2,3-diphosphoglycerate have been used to monitor intracellular pH of erythrocytes whose hemoglobin has been liganded with carbon monoxide.

T. Rink, R. Tsien, T. Pozzan

Measurements have been made of cytoplasmic pH, (pHi) and free Mg2+ concentration, ( [Mg2+]i), in pig and mouse lymphocytes. pHi was measured in four ways: by a digitonin null-point technique; by direct measurement of the pH of freeze-thawed cell pellets; from the 31P nuclear magnetic resonance (NMR) spectrum of intracellular inorganic phosphate; and by the use of a newly synthesized, intracellularly- trappable fluorescent pH indicator. In HEPES buffered physiological saline with pH 7.4 at 37 degrees C, pHi was close to 7.0. Addition of physiological levels of HCO3- and CO2 transiently acidified the cells by approximately 0.1 U. Mitogenic concentrations of concanavalin A (Con A) had no measurable effect on pH in the first hour. [Mg2+]i was assessed in three ways: (a) from the external Mg2+ null-point at which the ionophore A23187 produced no net movement of Mg2+ or H+; (b) by Mg- sensitive electrode measurements in freeze-thawed pellets; and (c) from the 31P nuclear magnetic resonance spectrum of the gamma-phosphate of intracellular ATP. Total cell Mg2+ was approximately 12 mmol per liter cell water. The NMR data indicated [Mg2+]i greater than 0.5 mM. The null-point method gave [Mg2+]i approximately 0.9 nM. The electrode measurements gave 1.35 mM, which was thought to be an overestimate. Exposure to mitogenic doses of Con A for 1 h gave no detectable change in total or free Mg2+.

W. Boron, P. De Weer

The intracellular pH (pHi) of squid giant axons has been measured using glass pH microelectrodes. Resting pHi in artificial seawater (ASW) (pH 7.6-7.8) at 23 degrees C was 7.32 +/- 0.02 (7.28 if corrected for liquid junction potential). Exposure of the axon to 5% CO2 at constant external pH caused a sharp decrease in pHi, while the subsequent removal of the gas caused pHi to overshoot its initial value. If the exposure to CO2 was prolonged, two additional effects were noted: (a) during the exposure, the rapid initial fall in pHi was followed by a slow rise, and (b) after the exposure, the overshoot was greatly exaggerated. Application of external NH4Cl caused pHi to rise sharply; return to normal ASW caused pHi to return to a value below its initial one. If the exposure to NH4Cl was prolonged, two additional effects were noted: (a) during the exposure, the rapid initial rise in pHi was followed by a slow fall, and (b) after the exposure, the undershoot was greatly exaggerated. Exposure to several weak acid metabolic inhibitors caused a fall in pHi whose reversibility depended upon length of exposure. Inverting the electrochemical gradient for H+ with 100 mM K- ASW had no effect on pHi changes resulting from short-term exposure to azide. A mathematical model explains the pHi changes caused by NH4Cl on the basis of passive movements of both NH3 and NH4+. The simultaneous passive movements of CO2 and HCO3-cannot explain the results of the CO2 experiments; these data require the postulation of an active proton extrusion and/or sequestration mechanism.

R. Zepp

G. Aad, E. Abat, B. Abbott et al.

G. Bayatian, S. Chatrchyan, G. Hmayakyan et al.

Mohammed Baalousha

G. S. Jadhav, M. Ghangrekar

S. Ikawa, K. Kitano, S. Hamaguchi

Yi-Fei Xia, Zi-Xiang Xu, Yu-Ting Yan et al.

Non-Hermitian systems have recently shown new possibilities to manipulate wave scattering by exploiting loss, yet coherent perfect absorption at an exceptional point (CPA EP) remains elusive in acoustics. Here we demonstrate it based on a two-channel waveguide with compact lossy resonators. We realize imbalanced losses crucial for CPA EP by using active components to independently modulate the non-Hermiticity. The CPA EP experimentally manifests as full absorption at a unique real frequency and shows high sensitivity to the incident phase variations.Our findings open an avenue to explore novel non-Hermitian physics for classical waves and develop innovative acoustic singularity-based devices.

Iuliia Timankova, Mikhail Smagin, Mikhail Kuzmin et al.

Controlling the directionality of the acoustic scattering with single acoustic metaatoms has a key importance for reaching spatial routing of sound with acoustic metamaterials. In this paper, we present the experimental demonstration of the acoustic analogue of the Kerker effect realized in a two-dimensional coiled-space metaatom. By engineering the interference between monopolar and dipolar resonances within a high-index acoustic metaatom, we achieve directional scattering with suppressed backward or forward response at the first and second Kerker conditions respectively. Experimental measurements of the scattered pressure field, in a parallel-plate waveguide environment, show good agreement with the full-wave simulations. Our results validate the feasibility of Kerker-inspired wave control in acoustic systems and open new opportunities for directional sound manipulation.

Binbin Liu, P. Mørkved, Å. Frostegård et al.

The N(2)O : N(2) product ratio of denitrification is negatively correlated with soil pH, but the mechanisms involved are not clear. We compared soils from field experiments where the pH had been maintained at different levels (pH 4.0-8.0) by liming (> or = 20 years), and quantified functional gene pools (nirS, nirK and nosZ), their transcription and gas kinetics (NO, N(2)O and N(2)) of denitrification as induced by anoxic incubation with and without a carbon substrate (glutamate). Denitrification in unamended soil appeared to be based largely on the activation of a pre-existing denitrification proteome, because constant rates of N(2) and N(2)O production were observed, and the transcription of functional genes was below the detection level. In contrast, glutamate-amended soils showed sharp peaks in the transcripts of nirS and nosZ, increasing the rates of denitrification and pH-dependent transient accumulation of N(2)O. The results indicate that the high N(2)O : N(2) product ratio at low pH is a post-transcriptional phenomenon, because the transcription rate of nosZ relative to that of nirS was higher at pH 6.1 than at pH 8.0. The most plausible explanation is that the translation/assembly of N(2)O reductase is more sensitive to low pH than that of the other reductases involved in denitrification.

Guillermo Chacon Acosta, Hector Hernandez Hernandez, Jose Ruvalcaba Rascon

We describe a quantum particle constrained on a catenoid, employing an effective description of quantum mechanics based on expected values of observables and quantum dispersions. We obtain semiclassical trajectories for particles, displaying general features of the quantum behaviour; most interestingly, particles present tunneling through the throat of the catenoid, a characteristic having important physical applications

M. Rostkowski, M. Olsson, Chresten R. Søndergaard et al.

BackgroundCharge states of ionizable residues in proteins determine their pH-dependent properties through their pKa values. Thus, various theoretical methods to determine ionization constants of residues in biological systems have been developed. One of the more widely used approaches for predicting pKa values in proteins is the PROPKA program, which provides convenient structural rationalization of the predicted pKa values without any additional calculations.ResultsThe PROPKA Graphical User Interface (GUI) is a new tool for studying the pH-dependent properties of proteins such as charge and stabilization energy. It facilitates a quantitative analysis of pKa values of ionizable residues together with their structural determinants by providing a direct link between the pKa data, predicted by the PROPKA calculations, and the structure via the Visual Molecular Dynamics (VMD) program. The GUI also calculates contributions to the pH-dependent unfolding free energy at a given pH for each ionizable group in the protein. Moreover, the PROPKA-computed pKa values or energy contributions of the ionizable residues in question can be displayed interactively. The PROPKA GUI can also be used for comparing pH-dependent properties of more than one structure at the same time.ConclusionsThe GUI considerably extends the analysis and validation possibilities of the PROPKA approach. The PROPKA GUI can conveniently be used to investigate ionizable groups, and their interactions, of residues with significantly perturbed pKa values or residues that contribute to the stabilization energy the most. Charge-dependent properties can be studied either for a single protein or simultaneously with other homologous structures, which makes it a helpful tool, for instance, in protein design studies or structure-based function predictions. The GUI is implemented as a Tcl/Tk plug-in for VMD, and can be obtained online at http://propka.ki.ku.dk/~luca/wiki/index.php/GUI_Web.

Vadapalli Surya Prasanth, Arun K. Singh

This research article presents stress distribution and fracture analysis of a cantilever beam considering both continuum and lumped distribution of gravity force. Airy stress function is used to derive two-dimensional stress distribution using power series and boundary conditions. It is concluded that bending stress is the most dominant over other two stresses. However, variation of vertical normal stress does not match between the two systems owing to change in load distribution. Moreover, an expression for energy release rate is also derived by assuming that a crack is present at the top of fixed end of beam and propagates vertically down to result in catastrophic failure. Finally, these results are validated with finite element simulations as well.

Fei Wang, K. Shih

Soumya Das, M. Jim Hendry, Joseph Essilfie-Dughan

Svend-Age Biehs, Philippe Ben-Abdallah

We investigate the adiabatic evolution of thermal state in non-reciprocal many-body systems coupled to their environment and subject to periodic drivings. In such systems we show that besides the dynamical phase a geometrical phase can exist and it drives the relaxation dynamic of the system. On the contrary to the dynamical phase which always pushes the system toward its equilibrium state we show that the geometric phase can speed up or reduce the speed of relaxation process. These results could have applications in the field of thermal management of complex systems.

Yi Feng, Robert Joynt

The valley degeneracy in Si qubit devices presents problems for their use in quantum information processing. It is possible to lift this degeneracy by using the Wiggle Well architecture, in which an oscillatory Ge concentration couples the valleys. This paper presents the basic theory of this phenomenon together with model calculations using the empirical pseudopotential theory to obtain the overall magnitude of this effect and its dependence on the wavelength of the concentration oscillations. We derive an important selection rule which can limit the effectiveness of the Wiggle Well in certain circumstances.