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DOAJ Open Access 2026
Environmental and demographic risk factors for respiratory illness in endangered wild mountain gorillas (Gorilla beringei beringei) in Rwanda

Kirsten V. K. Gilardi, Kirsten V. K. Gilardi, Brooke N. Genovese et al.

Morbidity and mortality from infectious diseases are an important health consideration for free ranging great apes, and respiratory illness (RI) is of particular concern in mountain gorillas (Gorilla beringei beringei), accounting for 25% all of mortalities. In addition to ongoing efforts to determine the etiology of RI in mountain gorillas, an improved understanding of environmental and demographic risk factors for the development of RI is critical for informing a disease prevention strategy for this species. To evaluate potential risk factors for RI, data were compiled from medical and life history records on free-ranging human-habituated mountain gorillas in Rwanda over a 7-year period from December 2005-December 2012. Associations between demographic, environmental and management risk factors and the presence of signs of clinical RI were evaluated using a multivariable mixed-effects logistic regression model. Clinical records from 21 habituated gorilla family groups, including 346 individuals, were included in analyses. Seventy-one percent of groups (n=15/21 gorilla groups) and 55% of individuals (n=192/346 gorillas) experienced at least one RI event. Rainy season (odds ratio [OR]=2.30, 95% CI = 1.74-3.03, P-value<0.001) and group size (OR = 1.15, 95% CI = 1.11-1.18, P-value<0.001) were associated with increased odds of RI in mountain gorillas, and there was a significant trend of increasing odds of RI with increasing group size for groups larger than 16 individuals and a more than 3-fold increase in odds of RI for groups larger than 38 individuals compared to groups of ten or less.

General. Including nature conservation, geographical distribution
DOAJ Open Access 2026
Acute and chronic effects of salinity on nitrification in a recirculating aquaculture system with açaí seeds (Euterpe oleracea) as biological media

Deyselana Lima da Costa, Bruno José Corecha Fernandes Eiras, Jean Danilo da Silva Pereira et al.

IntroductionThe Blue Amazon represents an extensive coastal zone with high biodiversity and wide salinity variation, which poses challenges for marine fish farming, particularly regarding the efficiency of biofilters in recirculating aquaculture systems (RAS). In this context, açaí seeds (Euterpe oleracea), previously evaluated in freshwater, emerge as a promising alternative for use as filter media under different salinity conditions. The objective of this study was to evaluate the potential of açaí seeds as substrate in RAS biofilters, analyzing their acute and chronic impact on the physicochemical parameters of water and the removal of ammonia, nitrite and nitrate over 28 days.MethodsThe experiment was conducted in six independent systems (three aquaria each), subjected to salinities of 0, 7, 14, 21, 28 and 35‰. After Acute (0, 20, 40, 60,80,100 and 120 minutes) and chronic (2,3,4,14,21 and 28 days) salinity change, water samples were collected to measure physicochemical quality and to assess nitrification efficiency and nitrogen compound removal.ResultsHigher oxygen consumption and ammonia clearance were observed at 0, 7 and 14‰ after 120 minutes of salinity change, while nitrate accumulation was significantly higher in freshwater. In long term, after 28 days, ammonia clearance was significantly lower at 35‰, though nitrate accumulation was not affected by salinity. The highest ammonia removal rates were recorded in the 0‰ and 7‰ treatments.DiscussionThe results demonstrate that açaí seeds are capable of removing ammonia after a few minutes and can sustain the growth of nitrifying bacteria under different salinity levels, although more efficiently in low salinity waters (seven times).

Science, General. Including nature conservation, geographical distribution
DOAJ Open Access 2025
Fishery-independent camera surveys provide novel observations of white sharks (Carcharodon carcharias) off coastal Alabama

J. Marcus Drymon, J. Marcus Drymon, Amanda E. Jargowsky et al.

Determining the distributions of marine animals is a challenge, particularly for highly migratory species like sharks. In the United States, several shark populations are beginning to recover following exploitation, including the white shark (Carcharodon carcharias). Recently, reports of white sharks have increased in the northern Gulf of Mexico (hereafter Gulf), but determining whether these reports represent actual changes in distribution is difficult. Therefore, we examined two long-term fishery-independent camera datasets to assess whether the recent increase in reports of white sharks reflects changes in distribution or typical (albeit rare) events. Long-term fishery-independent stereo-baited remote underwater video (sBRUV) and remotely operated vehicle (ROV) datasets were examined. From 2010 – 2024, 8368 sBRUV and 2199 ROV surveys were conducted. From 2010 – 2023, no white sharks were seen on either camera gear, but two white sharks were observed on ROV videos in 2024. The first was a female estimated at 239 cm total length and the second was a male estimated at 191 cm total length. These observations lend support to the notion that recent reports of white sharks in the northern Gulf may indicate early evidence of range-edge expansion rather than increases in telemetry efforts or citizen observations. Studies like this one highlight the value of long-term, randomized, fishery-independent camera surveys for documenting changes in distribution for rare species.

Science, General. Including nature conservation, geographical distribution
DOAJ Open Access 2025
Investigating plankton size spectra, biomass, abundance, and community composition in the Subtropical Convergence Front in the Southern Ocean

Natalia Yingling, Karen E. Selph, Moira Décima et al.

Phytoplankton community structure is crucial to pelagic food webs and biogeochemical processes. Understanding size-based biomass distribution and carbon dynamics is essential for assessing their contributions to oceanic carbon cycling. This study quantifies plankton carbon (C) based size spectra, community composition, living to total particulate organic carbon (POC) and C:Chlorophyll a (C:Chla) ratios across biogeographical provinces in the Pacific sector of the Southern Ocean near the Subtropical Front (Chatham Rise, Aotearoa-New Zealand). We analyzed phytoplankton community composition using epifluorescence microscopy and flow cytometry, while quantifying size-fractionated Chl-a and POC to estimate normalized biomass, abundance size spectra, and C:Chla ratios. On average, subtropical-influenced waters had lower macronutrients, higher total Chla (1.1 ± 0.2 μg Chla L-1) and were dominated by nanoplankton, which accounted for 45% of the total plankton community (35.2 ± 4.6 μg C L-1). In contrast, picoplankton dominated plankton communities within the subantarctic-influenced and accounted for 35% of the total plankton community (18.5 ± 0.9 μg C L-1) in these water with higher macronutrient concentrations and lower total Chla concentrations (0.32 ± 0.06 μg Chla L-1). Subantarctic-influenced regions had steeper (more negative) slopes for the normalized biomass size spectrum (average = -1.00) compared to subtropical-influenced waters (average = -0.78) indicating greater relative dominance of small taxa. The subantarctic-influenced region had ~2-fold higher surface average C:Chla ratios compared to the subtropical-influenced region with picoplankton consistently having lower C:Chla ratios, due to low Chla values, than larger nano- or microplankton. Live plankton carbon contributed a median of 67% of total particulate organic carbon in the euphotic zone (non-living detritus comprises the remaining ~1/3), which is indicative of substantial primary production and rapid recycling by a strong microbial loop. Our study provides important insights into phytoplankton community structure, biomass distribution and their contribution to carbon sequestration in this region, highlighting the important roles of nanoplankton in subtropical productive waters and picoplankton in offshore subantarctic waters as well as a strong variation of C:Chla across different phytoplankton size classes.

Science, General. Including nature conservation, geographical distribution
DOAJ Open Access 2024
Application of the MENTOR model to advance One Health by promoting bat conservation and reducing zoonotic spillover risk

Lindsay J. Smith, Nancy Gelman, M. Teague O’Mara et al.

For few taxonomic groups do conservation efforts have such a disproportionate impact on biodiversity and human well-being as they do with bats. Bats face significant conservation challenges that affect their long-term viability, inhibit their ecosystem functions and services, and increase zoonotic spillover risks. Protecting bat populations and their habitats ultimately reduces these conservation threats, helps prevent pandemics, and supports essential ecosystem services. MENTOR-Bat is a fellowship program focused on strengthening technical research, and leadership capacity in the Global South to promote healthy environments where bats and humans can coexist with reduced risks of pathogen transmission. Co-designed by the United States Fish and Wildlife Service (USFWS) and Bat Conservation International (BCI), MENTOR-Bat mirrors the One Health framework by featuring a transdisciplinary team of three mentors and nine fellows from Cameroon, Colombia, and Indonesia. Fellows and mentors receive academic and field-based training on bat ecology and conservation, One Health, human dimensions of conservation, behavior change, strategic communications, international policy, adaptive management, project planning, conservation leadership, and public health. Fellows will then design and implement team pilot projects to advance One Health and bat conservation in their respective countries. Program evaluation of MENTOR-Bat is based on Kirkpatrick’s Hierarchy and focuses on measuring the development of established One Health core competences. By incorporating One Health and conservation within its activities, MENTOR-Bat can become a valuable programmatic template for transdisciplinary programming advancing evidence-based strategies for improving the well-being of bats, humans, and the environment.

General. Including nature conservation, geographical distribution
DOAJ Open Access 2024
Far away from home? Ancient DNA shows the presence of bicolored shrew (Crocidura leucodon) in Bronze Age Denmark

Mahsa Mousavi‐Derazmahalleh, Niels Haue, Marie Kanstrup et al.

Abstract An excavation of an Early Iron Age village near Aalborg in Denmark uncovered the jaws and skull fragments from a small mammal that were morphologically identified to the genus Crocidura (white‐toothed shrews). Three Crocidura species are known from prehistoric continental Europe but none of them are distributed in Scandinavia, which is why this surprising finding warranted further analyses. The bone was radiocarbon‐dated to 2840–2750 calibrated years before present (cal. BP), corresponding to the Late Bronze Age and hence earlier than the Iron Age archeological context in which it was found. Using highly optimized ancient DNA protocols, we extracted DNA from one tooth and shotgun‐sequenced the sample to reconstruct a near‐complete mitochondrial reference genome (17,317 bp, 32.6× coverage). Phylogenetic analyses determined this specimen as a bicolored shrew (Crocidura leucodon) but with a phylogenetic position basal to the clade of known sequences from this species. The confirmation of Crocidura presence in Denmark by the Late Bronze Age sheds new light on the prehistoric natural history of Scandinavia. We discuss the implications of this finding from both zoo‐archeological and ecological perspectives. Furthermore, the mitochondrial genome reconstructed in this study offers a valuable resource for future research exploring the genetic makeup and evolutionary history of Eurasian shrew populations.

DOAJ Open Access 2024
Culls effective for reducing mute swans in New Jersey

Theodore C. Nichols, Lisa A. Clark

Abstract Mute swans (Cygnus olor) have increased in New Jersey at ~7%/yr since the mid‐1980s with the population peaking at 1,890 mute swans by 2005. Wildlife agencies have implemented management programs to reduce mute swan numbers to population objectives; however, evaluation of these programs is lacking. Given the detrimental impacts of mute swans on wetland habitats, we implemented a cull program in 2007 in New Jersey through shooting, mostly during mid‐summer, and focused cull efforts on key molting sites. Our primary objective was to measure the impact of culls on mute swan population trends. We also examined population response of mute swans at key molting sites over time after culls began. We conducted 61 field operations at 21 sites and culled 2,038 mute swans over 11 years. We achieved a mean annual statewide cull rate of 0.15 and reduced the 2005 mute swan population 56% by 2017. The statewide mute swan population declined (P = 0.038) at nearly 8%/yr following commencement of culls. Four heavily used molting sites comprised 79% of the mute swans culled and population response varied across the 4 key cull sites. We fit a linear model using a derived annual cull rate and mute swan abundance data to describe the effect of culls on population growth (λ) and determined that a cull rate >0.09 was required to reduce (λ < 1) the mute swan population in New Jersey. Our work should benefit wildlife agencies who are considering implementing mute swan management programs.

General. Including nature conservation, geographical distribution
DOAJ Open Access 2023
PREFACE

Oleh Pylypchuk, Oleh Strelko, Yuliia Berdnychenko

We are delighted to welcome you to the new issue of the journal on the history of science and technology! This issue is unique as it explores diverse aspects of the development of science and technology in various countries and historical periods. We invite you on an exciting journey through the pages of this issue, where you will find works by distinguished scientists such as Maryna Gutnyk, Florian Nürnberger, Tetiana Karmadonova, Natalya Pasichnyk, Renat Rizhniak, Нanna Deforzh, Liudmyla Zhuravlova, and many others. Their research covers various facets of history and technology. The collaborative work by Maryna Gutnyk and Florian Nürnberger presents a comprehensive exploration of the evolution of the Fe-C diagram, tracing its historical development through the lenses of various scientific contributions over time. Their analysis underscores the rich history behind this diagram, highlighting the foundational studies dating back to the early 19th century, marking crucial milestones in understanding the carbon content in steel and its implications for industrial applications. The authors' meticulous use of comparative analysis, synthesis, and chronological examination sheds light on the gradual refinement and evolution of the Fe-C diagram. From the initial recognition of graphite as pure carbon to the establishment of phase diagrams through collaborative efforts at international congresses, the Fe-C diagram's progression intertwines with the advancements of the industrial revolution. Tetiana Karmadonova's work on the migration trends of Ukrainian researchers from 1991 to 2023 provides a comprehensive analysis of the multifaceted factors driving the migration of scientists from Ukraine to various destination countries, particularly against the backdrop of recent events in the country. The study delves into the intricate landscape of migration among Ukrainian researchers across different historical periods. Natalya Pasichnyk, Renat Rizhniak, and Нanna Deforzh's meticulous study on the publications in the "Bulletin of Experimental Physics and Elementary Mathematics" from 1886 to 1917 offers invaluable insights into the organization, proceedings, and outcomes of domestic and international congresses of mathematicians and natural scientists during that period. Their research, focused on a comprehensive and quantitative analysis of these journal publications, sheds light on the pivotal role of these gatherings in the scientific and pedagogical realms Liudmyla Zhuravlova's research on the evolution of techno-nationalism and the pivotal role of space in this phenomenon from the 1980s to the 2020s offers a compelling exploration into the intricate dynamics of technological advancements and their influence on international relations and national strategies. The article delves deeply into the theoretical comprehension of techno-nationalism, particularly examining its relationship with space policy and its relevance within the context of US-China relations. Employing an interdisciplinary approach, drawing from historical, economic, political sciences, and international relations theory, the research unravels the dichotomous evolution of techno-nationalism juxtaposed against techno-globalism. Zhuravlova's work accentuates the ongoing power struggle between the US and China within the space industry, amplifying the techno-nationalist dimensions within innovation systems. Artemii Bernatskyi and Mykola Sokolovskyi's research presents a comprehensive review of the evolution of additive manufacturing (AM) processes within the realm of metallurgy, spanning from the foundational theories of layer-by-layer manufacturing to the contemporary landscape of AM technologies. This work illuminates the rapid advancements within the AM sector, capturing the profound interest of the scientific community. It underscores the dual significance of AM technologies - not only as an alternative manufacturing method for existing structures but also as a gateway to crafting new, intricately complex structures unattainable through traditional methodologies. Through meticulous analysis and classification of prior studies focusing on technological advancements and implementations, the research establishes a structured approach towards comprehensively mapping the development of additive manufacturing technologies in various trajectories. As a result, the research proposes a systematic approach to formulate a comprehensive scheme for AM technology development, thereby offering a framework that navigates the intricate landscape of technological advancements in various directions. Mykhailo Klymenko's meticulous study offers a comprehensive evaluation of Professor Tomasz Nikodem Ścibor-Rylski's pioneering contributions to the development of agricultural machinery testing during the latter half of the 19th century. This research sheds new light on Rylski's scientific endeavors and their significant impact on the evolution of agricultural equipment testing. Employing principles of historicism, scientific rigor, and objectivity, Klymenko utilizes historical-scientific methodologies, archival analysis, and generalization to present a nuanced understanding of Rylski's work. For the first time, archival documents are introduced, unveiling insights into the scientist's activities in advancing the field of agricultural machinery testing. Mohamad Khairul Anuar Mohd Rosli, Ahmad Kamal Ariffin Mohd Rus, and Suffian Mansor's insightful study delves into the overlooked yet pivotal role of electricity, specifically facilitated by the Perak River Hydro-Electric Power Company (PRHEPC), in the tin-mining industry within Kinta Valley during the period of 1927 to 1940. The research illuminates the historical emergence of electricity as a dominant power source in the tin-mining industry of Colonial Malaya, a topic that has received minimal attention in Malaysian historiography. Sana Simou, Khadija Baba, and Abderrahman Nounah's research represents a profound call to action amidst the urgent need to safeguard Morocco's cultural heritage, notably exemplified by the Marinid Madrasa within the Chellah archaeological site in Rabat. This research intricately weaves advanced technologies with a profound appreciation for the historical, social, and cultural significance of these sites. It charts a course that not only conserves architectural brilliance but also honors the profound stories encapsulated across epochs. Ultimately, it emerges as a blueprint for harmonizing the past with the present, ensuring the preservation of cultural heritage while embracing the imperatives of progress. In his article, Oleh Strelko shows that the history of bridge construction is an important part of historical knowledge. Developments in bridge construction technology reflect not only engineering advances, but also social, economic and cultural aspects of society. Engineers and scientists faced unique challenges when designing and building bridges depending on the technological level of the era, available materials and the needs of society. This process may reflect technological progress, changes in transportation needs, and cultural and social changes. The purpose of this article is to briefly review key moments and stages in the history of metal bridge construction using welding technology in the 20th century. We invite you on this exciting journey with our authors exploring the history of science, technology, and cultural heritage. May this issue broaden your knowledge and inspire new research endeavors!

History (General) and history of Europe, Science (General)
DOAJ Open Access 2023
Reintroducing bison to Banff National Park – an ecocultural case study

Karsten Heuer, Jonathan Farr, Leroy Littlebear et al.

The reintroduction of extirpated species is a frequent tactic in rewilding projects because of the functional role species play in maintaining ecosystem health. Despite their potential to benefit both ecosystems and society, however, most well-known species reintroductions have adopted an eco-centric, “nature-in-people-out” approach. Rewilding theory and practitioners acknowledge that ignoring the role Indigenous people did and might once again play in shaping the distribution, abundance, movements, behavior, and health of wild species and ecosystems, is limiting. In this case study, we describe the technical steps we took and how Indigenous knowledge, ceremony, and cultural monitoring were woven into the recent reintroduction of plains bison to Canada’s Banff National Park. Six years later, the reintroduced bison herd has grown from 16 to &gt;100 animals, ranges mostly within 30 km of the release site, and, if current growth continues, will likely be managed with Indigenous harvesting. Transboundary bison policy differences are shifting and may lead to bison being more sustainable. The ecocultural approach, therefore, has increased the resilience of our rewilding project.

General. Including nature conservation, geographical distribution
DOAJ Open Access 2022
Why are some plant species missing from restorations? A diagnostic tool for temperate grassland ecosystems

Marcello De Vitis, Marcello De Vitis, Kayri Havens et al.

The U.N. Decade on Ecosystem Restoration aims to accelerate actions to prevent, halt, and reverse the degradation of ecosystems, and re-establish ecosystem functioning and species diversity. The practice of ecological restoration has made great progress in recent decades, as has recognition of the importance of species diversity to maintaining the long-term stability and functioning of restored ecosystems. Restorations may also focus on specific species to fulfill needed functions, such as supporting dependent wildlife or mitigating extinction risk. Yet even in the most carefully planned and managed restoration, target species may fail to germinate, establish, or persist. To support the successful reintroduction of ecologically and culturally important plant species with an emphasis on temperate grasslands, we developed a tool to diagnose common causes of missing species, focusing on four major categories of filters, or factors: genetic, biotic, abiotic, and planning &amp; land management. Through a review of the scientific literature, we propose a series of diagnostic tests to identify potential causes of failure to restore target species, and treatments that could improve future outcomes. This practical diagnostic tool is meant to strengthen collaboration between restoration practitioners and researchers on diagnosing and treating causes of missing species in order to effectively restore them.

General. Including nature conservation, geographical distribution
DOAJ Open Access 2022
Spatio-temporal nonconvex penalty adaptive chirp mode decomposition for signal decomposition of cross-frequency coupled sources in seafloor dynamic engineering

Qing Li

Electromagnetic field noise and clutter generated from the motion of ocean waves are the main obstacles in the research of magnetotelluric dynamic analysis, and it is difficult to extract the crossed instantaneous frequencies (IFs) of underwater electromagnetic detected (UEMD) data due to the limited resolution of the current time-frequency techniques. To alleviate this bottleneck issue, a new spatio-temporal nonconvex penalty adaptive chirp mode decomposition (STNP-ACMD) is originally proposed for separating each mono-component individually from a complicated multi-component with severely crossed IFs or overlapped components, in this paper. Specifically, the idea of a nonconvex penalty greedy strategy is incorporated into the vanilla ACMD method by using a recursive mode extraction scheme, and the fractional-order characteristic of the observation signal is also considered. Meanwhile, the spatio-temporal matrices were constructed elaborately and then applied to capture coupling characteristics and spatio-temporal relationships among all estimated mono-components. Eventually, a high-resolution adaptive time-frequency spectrum is obtained according to the IFs and instantaneous amplitudes (IAs) of each estimated mono-component. The effectiveness and practicability of the proposed algorithm were verified via simulated scenarios and velocity dynamic data of the seafloor from the South China Sea, compared with four state-of-the-art benchmarks.

Science, General. Including nature conservation, geographical distribution
DOAJ Open Access 2021
Remote, but Not Isolated—Microplastics in the Sub-surface Waters of the Canadian Arctic Archipelago

Kirstie Jones-Williams, Kirstie Jones-Williams, Tamara S. Galloway et al.

As the remote Canadian Arctic Archipelago (CAA) becomes increasingly connected to the rest of the world, there is an impetus to monitor the possible impact of this connectivity. The potential for increases in localised sources of plastic pollution resulting from the increasing navigability of the remote north has yet to be explored. Here we investigate microplastic samples which were collected aboard the Canadian Coast Guard Ship (CCGS) Amundsen in the summer of 2018 using the underway pump and a filtration system with Fourier transform infrared analysis. We investigate the character, abundance, and distribution of microplastic particles and fibres in the sub-surface waters across the Canadian Arctic and add to the limited dataset on plastic pollution in this region. We find that there are low concentrations of microplastics ranging from 0 to 0.282 n L–1 (average 0.031 ± 0.017 n L–1), comprising 71% polyester and acrylics. We investigate the size distribution of retained particles and fibres on three different filter mesh sizes connected to the underway pump (300, 100, and 50 μm) and find that a 300 μm mesh and a 100 μm mesh retain only 6 and 56%, respectively, of the total particles and fibres. We explore the role of shipping as a potential source of textile fibres and we suggest that future monitoring of plastics in the Canadian Arctic should use the current shipping fleet to monitor its own plastic footprint, utilising the underway pump and mesh sizes &lt; 100 μm.

Science, General. Including nature conservation, geographical distribution
DOAJ Open Access 2021
Validation of Oil Trajectory and Fate Modeling of the Deepwater Horizon Oil Spill

Deborah P. French-McCay, Malcolm L. Spaulding, Deborah Crowley et al.

Trajectory and fate modeling of the oil released during the Deepwater Horizon blowout was performed for April to September of 2010 using a variety of input data sets, including combinations of seven hydrodynamic and four wind models, to determine the inputs leading to the best agreement with observations and to evaluate their reliability for quantifying exposure of marine resources to floating and subsurface oil. Remote sensing (satellite imagery) data were used to estimate the amount and distribution of floating oil over time for comparison with the model’s predictions. The model-predicted locations and amounts of shoreline oiling were compared to documentation of stranded oil by shoreline assessment teams. Surface floating oil trajectory and distribution was largely wind driven. However, trajectories varied with the hydrodynamic model used as input, and was closest to observations when using specific implementations of the HYbrid Coordinate Ocean Model modeled currents that accounted for both offshore and nearshore currents. Shoreline oiling distributions reflected the paths of the surface oil trajectories and were more accurate when westward flows near the Mississippi Delta were simulated. The modeled movements and amounts of oil floating over time were in good agreement with estimates from interpretation of remote sensing data, indicating initial oil droplet distributions and oil transport and fate processes produced oil distribution results reliable for evaluating environmental exposures in the water column and from floating oil at water surface. The model-estimated daily average water surface area affected by floating oil &gt;1.0 g/m2 was 6,720 km2, within the range of uncertainty for the 11,200 km2 estimate based on remote sensing. Modeled shoreline oiling extended over 2,600 km from the Apalachicola Bay area of Florida to Terrebonne Bay area of Louisiana, comparing well to the estimated 2,100 km oiled based on incomplete shoreline surveys.

Science, General. Including nature conservation, geographical distribution
DOAJ Open Access 2017
A minimally-invasive method for sampling human petrous bones from the cranial base for ancient DNA analysis

Kendra A. Sirak, Daniel M. Fernandes, Olivia Cheronet et al.

Ancient DNA (aDNA) research involves invasive and destructive sampling procedures that are often incompatible with anthropological, anatomical, and bioarcheological analyses requiring intact skeletal remains. The osseous labyrinth inside the petrous bone has been shown to yield higher amounts of endogenous DNA than any other skeletal element; however, accessing this labyrinth in cases of a complete or reconstructed skull involves causing major structural damage to the cranial vault or base. Here, we describe a novel cranial base drilling method (CBDM) for accessing the osseous labyrinth from the cranial base that prevents damaging the surrounding cranial features, making it highly complementary to morphological analyses. We assessed this method by comparing the aDNA results from one petrous bone processed using our novel method to its pair, which was processed using established protocols for sampling disarticulated petrous bones. We show a decrease in endogenous DNA and molecular copy numbers when the drilling method is used; however, we also show that this method produces more endogenous DNA and higher copy numbers than any postcranial bone. Our results demonstrate that this minimally-invasive method reduces the loss of genetic data associated with the use of other skeletal elements and enables the combined craniometric and genetic study of individuals with archeological, cultural, and evolutionary value.

Biology (General)
DOAJ Open Access 2014
Taxonomic reassessment of two Indian shieldtail snakes in the <i>Uropeltis ceylanicus</i> species group (Reptilia: Uropeltidae)

S.R. Ganesh, R. Aengals, E. Ramanujam

Uropeltis is the most speciose of all shieldtail snake (uropeltid) genera, particularly in India, and has been bedeviled by a complex and intricate taxonomic history, with several weakly established synonyms and widely disjunct geographic ranges. Our present work on two Indian Uropeltis species revealed greater species diversity than what is currently recognised. We elevate Uropeltis arcticeps madurensis to species level, and revive Silybura shorttii (in the combination Uropeltis shorttii) from the subjective synonymy of U. ceylanicus. We provide differential diagnoses, descriptions of examined material and comparisons with similar species based on an examination of voucher specimens as well as fresh, uncollected topotypes documented in the field.

Ecology, General. Including nature conservation, geographical distribution
DOAJ Open Access 2012
A taxonomic revision of the southern African native and naturalized species of <i>Silene</i> L. (Caryophyllaceae)

J. C. Manning, P. Goldblatt

The native and naturalized species of Silene L. in southern Africa are reviewed, with full synonomy and the description of two new species from the West Coast of Western Cape. Eight native species and three naturalized species are recognized, including the first identification in southern Africa of the Mediterranean S. nocturna L. The identity of S. aethiopica Burm., which has remained unknown since its description, is established and is found to be the oldest name for S. clandestina Jacq. Patterns of morphological variation within each species are discussed and subspecies are recognized for geographically segregated groups of populations that are ± morphologically diagnosable. The following new names or combinations are made among the southern African taxa: S. aethiopica subsp. longiflora; S. burchellii subsp. modesta, subsp. multiflora, and subsp. pilosellifolia; S. crassifolia subsp. primuliflora; S. saldanhensis; S. rigens; and S. undulata subsp. polyantha. Each taxon is described, with information on ecology and distribution, and most species are illustrated, including SEM micrographs of the seeds.

General. Including nature conservation, geographical distribution
DOAJ Open Access 1976
Notes on the frog fauna of the Amazon Basin

W. Ronald Heyer

Abstract The natural history of the frog fauna of the Amazon Basin is considered, based on data obtained from four major collections. Population structures in both forest and open environments are compared. Associations of species pairs in open formations are studied. It is estimated that at least 100 species are present in the Amazon Basin. Five speciation models are proposed to explain the high diversity of species.

Science (General)
DOAJ Open Access 1994
Breeding systems in some representatives of the genus <i>Lycium</i> (Solanaceae)

L. Minne, J. J. Spies, H. J. T. Venter et al.

The development of the ovule and the embryo sac of five of the 17 species of Lycium and of one hybrid, recorded for southern Africa, was investigated. All specimens of four of the species and the hybrid (between a hermaphroditic and a functionally dioecious species) were found to be functionally dioecious: they express only one sex, although both male and female organs are present in the same tlower. One species was hermaphroditic. The embryo sacs of all species, and of the hybrid, were of the normal eight-nucleate Polygonum type. The structure of the ovary and the development of the embryo sac are similar to those of L europaeum L. The absence of unreduced embryo sacs indicates that apomixis does not occur at any ploidy level in the species studied.

General. Including nature conservation, geographical distribution
DOAJ Open Access
Réplique

Leo Wehrli, Carl Burckhardt

Dans la partie littéraire de «Petermanns geographische Mitteilun-gen», 1898, nous trouvons, signé par A. Tornquist,. quelques remarques concernant le rapport préliminaire de notre premiére expédition dans la Cordillére. D'un cóté, ces remar ques nous attribuent des opinions que nous n'avons pas émises: de l'autre cóté, elles nous attaquent; nous nous sentons donc obligés á donner les justifications suivantes: A.   Rectifications géologiques. 1 ) M. Tornquist, dans son compte-rendu, parle de diorites pré-jurassiques. Non; nous n'avons pas dit cela; mais nos diorites sont jurassiques ou post-jurassiques; car elles percent les sédiments jurassiques en forme de filons ou y sont intercalées comme «Lagergang». L'éruption de ces diorites est nettement postérieure à la sédimentation des couches jurassiques avec lesquelles nous les avons trouvées en contact. 2) A l'inverse, nous n'avons pas décrit d'andésites et basaltes «déjá jurassiques». comme nous le fait diré M. Tornquist. Ces roches sont plus modernes. Mais leurs centres d'éruption se trouvent, parfois, en coíncidence locale avec des centres éruptifs du temps jurassique. Cependant ceux-ci n'étaient point des magmas basaltiques, mais plutót porphyriques  et porphyritiques (páléovolcaniques!). Quant á leur relation chimique avec les laves néovolcaniques, nous y reviendrons Dans notre rapport principal.

Anthropology, Natural history (General)

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