Hasil untuk "Auxiliary sciences of history"

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DOAJ Open Access 2025
Non-Invasive Preservation Assessment of Archaeological Animal Bones by Complementary Imaging Techniques

Chloe Pearce, Fabien Léonard, Oxana V. Magdysyuk et al.

The preservation of archaeological bone is of great importance for both archaeological and conservation science studies. Traditional methods of preservation assessment, such as attenuated total reflectance Fourier transform infrared spectroscopy (ATR-FTIR), are minimally invasive and destructive. Neutron and X-ray tomography offer a totally non-invasive novel analysis method for the state of preservation of archaeological bones. Seven archaeological animal bones were selected for analysis based on animal maturity, species, visual factors, and ATR-FTIR analysis results. Archaeological bone is a hierarchical composite material constructed from both organic and mineral components; therefore, neutron tomography and synchrotron X-ray tomography have been combined in this novel approach to assess the state of preservation of animal archaeological bone. The neutron data demonstrated that the organic distribution along the diaphysis of archaeological bones varied significantly both within bones and between different animal bones. There is minimal consistency between the samples, emphasizing the inhomogeneity in archaeological bone collections. X-ray tomography revealed unseen physical details, including cracks and substantial damage. The collection of this information via non-invasive methods is highly valuable for cultural heritage, providing a deeper understanding of the observed inhomogeneity in ATR-FTIR analysis data and revealing obscured physical details.

S2 Open Access 2025
The evolution of velocity dispersion in the Sco-Cen OB association

J. Großschedl, J. Alves, S. Ratzenbock et al.

We study how the stellar velocity dispersion within the Scorpius-Centaurus OB association (Sco-Cen) has evolved over approximately 20 million years, from its formation to the present day, by investigating 32 stellar clusters in Sco-Cen. Using data from the mission along with supplementary stellar radial velocities, we identified a surprising sequence of abrupt jumps and intervening plateaus in the evolution of velocity dispersion correlating with times of star formation bursts. Gaia -1 . We measure a present-day expansion rate of about 10--12,pc,Myr -1 and observe that younger star clusters within the association exhibit higher velocities compared to older ones. This result, along with the stepwise increase in velocity dispersion over time, suggests a structured and sequential star formation process rather than a random one. This phased evolution suggests that stellar feedback is the primary driver of Sco-Cen's star formation history, expansion, and eventual dispersal. Our findings emphasise the value of precisely characterising stellar populations within OB associations, particularly through the creation of detailed, high-resolution age maps.

S2 Open Access 2025
Forecasting financial distress in dynamic environments AI adoption signals and temporally pruned training windows

Frederik Rech, Hussam Musa, Martin vSebevna et al.

Forecasting corporate financial distress increasingly requires capturing firms'adoption of transformative technologies such as artificial intelligence, yet model performance remains vulnerable to temporal distribution shifts as these technologies diffuse. This study investigates whether firm-level artificial intelligence (AI) adoption proxies improve forecasting performance beyond standard accounting fundamentals. Using a panel of Chinese A-share non-financial firms from 2007 to 2023, we construct AI indicators from textual disclosures and patent data. We benchmark six machine learning classifiers under a strictly chronological design that fixes the final test year and progressively prunes the training history to capture temporal change. Results indicate that AI proxies consistently improve out-of-sample discrimination and reduce Type II errors, with the strongest gains in tree-based ensembles. Predictive performance is non-monotonic in training window length; models trained on recent data outperform those using full history, while single-year training proves unreliable. Explainability analyses reveal financial ratios as primary drivers, with AI adoption signals adding incremental forecasting content whose interpretation as a risk factor varies across training regimes. Our findings establish AI proxies as valuable predictors for distress screening and demonstrate that adaptive, temporally pruned forecasting windows are essential for robust early warning models in rapidly evolving technological and economic environments.

en Economics
CrossRef Open Access 2025
Employing auxiliary natural lighting systems within the elements of traditional buildings: A special study on employing one of the auxiliary lighting techniques within one of the elements of a mosque in the ancient urban fabric

Alaa Hadi Ubaid

Technological development is considered one of the most important influences that contributed to the change of some design elements (formal and functional), both or each. Among these elements is the mina-ret. This was evident in the old urban fabric, and because of the existence of other basic reasons in the tradi-tional fabric, including the increase in the population of the weaving area, which led to the dispensation of the inner courtyard. It had changed from an element that has a shape and function to an element that has a shape only, to indicate the presence of a mosque this was evident in the old urban fabric .Due to the pres-ence  of other essential causes in the traditional fabric like the increase in population among the inhabitants of the fabric which led to  neglecting  the internal area , moreover climate change led to the use of window openings as outlets for air conditioning instruments ,the researcher employed the minaret to enable sunlight  inter the inner space of the mosque (prayer space) due to the importance of natural sunlight effect on peo-ple's psychological and physiological  health . This research was conducted in a clear sky of Babil province for latitude of (32.8) north, small samples were made measuring 1:20 for the minaret and the mosque space, a device for measuring the sunlight amount  (Multi-Function Environment Meter 4IN1) was used . Results showed an increase in natural sunlight reflected inside the space of the mosque. This study came out with the following recommendations, the importance of employing the archeological elements which their func-tions were altered by technological growth to enhance the internal environment with the finding of solutions to the amount of harmony in distributing sunlight (distributing natural sunlight harmoniously )

S2 Open Access 2024
Euclid: Early Release Observations of diffuse stellar structures and globular clusters as probes of the mass assembly of galaxies in the Dorado group

M. Urbano, P. Duc, T. Saifollahi et al.

Deep surveys have helped to unveil the history of past and present galaxy mergers, and, in particular, uncovering their tidal debris and co-located globular clusters (GCs). Euclid's unique combination of capabilities (spatial resolution, depth, and wide sky coverage) will make it a groundbreaking tool for galactic archaeology in the Local Universe, bringing low-surface-brightness (LSB) science into the era of large-scale astronomical surveys. Euclid's Early Release Observations (ERO) demonstrate this potential with a field of view that includes several galaxies in the Dorado group. In this paper, we aim to derive from this image a mass assembly scenario for its main galaxies: NGC,1549, NGC,1553, and NGC,1546. We detected their internal and external diffuse structures, and identified candidate GCs. By analysing the colours and distributions of the diffuse structures and candidate GCs, we can place constraints on the galaxies' mass assembly and merger histories. The results demonstrate that feature morphology, surface brightness, colours, and GC density profiles are consistent with galaxies that have undergone different merger scenarios. We classify NGC,1549 as a pure elliptical galaxy that has undergone a major merger. NGC,1553 appears to have recently transitioned from a late-type galaxy to early type, after a series of radial minor to intermediate mergers. NGC,1546 is a rare specimen of galaxy with an undisturbed disk and a prominent diffuse stellar halo, which we infer has been fed by minor mergers and then disturbed by the tidal effect from NGC,1553. Finally, we identify limitations specific to the observing conditions of this ERO, in particular, stray light in the visible and persistence in the near-infrared bands. Once these issues are addressed and the extended emission from LSB objects is preserved by the data-processing pipeline, the Euclid Wide Survey will allow for studies of the Local Universe to be extended to statistical ensembles over a large part of the extragalactic sky.

1 sitasi en Physics
S2 Open Access 2024
The Battle at Demetrias in the Thessalian Campaign of John Palaiologos: A Military Historical Reconstruction

V. Zolotovskiy

Introduction. The author proposes a new reconstruction of the military-political conditions for the organization of the Thessalian campaign of John Palaiologos, as well as the major naval battle in the history of the Late Byzantine Empire that took place during it. The warfare between the Latin and Byzantine fleets at Demetrias is of interest due to the possibility of battle practice analysis in the context of the formation of the early Palaiologian military-strategic concept and the development of late Byzantine military art in general. Methods. The methodological basis of the work is the critical use of elements of systemic approaches. It should be noted that the system approach allows us to reveal the role and level of mutual influence of political and socio-economic factors that determined the specifics of military organisation and the development of military science and battle practice. Analysis and results. After the renewal of the church union discussion, hoping for the support of Clement IV in the war of the anti-imperial forces, the Byzantines organized a new large-scale campaign in Thessaly. In the spring of 1273, the army of many thousands was sent there. An analysis of the sources that reported detachments about the second stage of the campaign showed that along with the decision to send the French knights to Neopatras, the Lombard barons of Euboea began to form a flotilla. The purpose of the squadron being formed was to inflict a crushing defeat on Byzantium at sea. The reconstruction of the battle at Demetrias showed that the Byzantines were not prepared for the naval battle that had begun. This was due to the fact that the fleet under the command of Philanthropenos, within the framework of the Thessalian campaign, only an auxiliary role was assigned. It could equally act as a cover for the land forces from potential assistance from the Euboean barons and also be used to reshuffle a large land contingent to the area of the main warfare. The analysis of the naval battle showed that the Byzantine army’s military successes were based not only on the talent of the commanders who commanded the military troops during the period of the military organization degradation. In such a significant battle, the defeat of which the entire imperial fleet was threatened with destruction, Philanthropenos’s squadron won thanks to professional training and combat experience, which was based on knowledge of Byzantine polemology. The outcome of the battle was influenced by the composition and number of the ground forces involved, as well as the use of stratagems.

S2 Open Access 2024
Metaphor as a Factor of Evolutionary Variability of Scientific Theories

A. Zharov

The article analyzes philosophical problems related to the study of the verbalization of new knowledge. Special attention is given to identifying the place and role of metaphor in the linguistic expression of this knowledge. The originality of metaphorical speech in various fields of creativity (science, poetry, and mythology) is characterized. The analysis of the features of the metaphorical language of science is conducted mainly through the history of development of physical knowledge. The author aims to develop his own generalizing concept of metaphor based on Russian philosopher Alexander Nikiforov’s theory about the structure of human cognition, consisting of three contexts (material, cultural, and personal) with the elements of which a scientist compares a new phenomenon. In this regard, the essence of the metaphor lies in the interplay of similarities and differences between the scientist’s personal experience and new data. Ultimately, new knowledge is formed with the establishment of a metaphor that interprets the new phenomenon. Initially, a so-called root metaphor is formulated, expressing the main aspect of the new knowledge. Subsequently, a network of clarifying auxiliary metaphors develops around it, interlocking due to the increasing interaction of various aspects of the scientist’s personal experience with different aspects of a new phenomenon being framed into new knowledge. In this case, the metaphor acts as an integral element of the conceptual populations, according to Stephen Toulmin. The content of these populations has not sufficient elaborated in the British philosopher’s teaching, and the author of the article addresses this gap. From his perspective, it is the metaphor that can act as a basic element of such populations, thereby becoming the primary measure of evolutionary variability of scientific theories. In addition, the author believes that the metaphorical definition of Toulmin’s basic element of conceptual populations can resolve conflicts between different approaches to describing scientific progress. Focusing on the description of its nature rather than on the extent of variability of scientific knowledge will allow the conflicting parties to unite.

1 sitasi en
S2 Open Access 2024
Mediumship and stylometry: Exploring a new way of attributing authorship to mediumistic writings

Adrian Weibel

Stylometry is still largely unexplored for applications in attributing authorship to mediumistic writings, as outlined in the subsequent literature review. Stylometry is a statistical method for analyzing authorship with a history to detect anonymous authors, e. g., J. K. Rowling as the real author of The Cuckoo’s Calling. Modern stylometry transcends literature analysis, applying to forensic inquiries, such as plagiarism detection, online security, and group identity characteristics. Historically, stylometric research unfolds in three phases: focusing on single numerical functions, statistical methods based on word frequencies, and contemporary use of machine learning. Authorship analysis includes three primary objectives: authorship attribution, verification, and characterization. It uses mathematical linguistics, information theory, and linguistic features like vocabulary richness, word frequency, and syntactic structures. Stylometric methods reliably detect authorship in imitation scenarios (pastiche) and have now also led to adversarial stylometry, which facilitates and identifies attempts at anonymization. Distribution patterns of features such as function words or punctuation can only be detected with high computing power and appear to contain too little content or emotion to be recognized by psi abilities of mediums. Imitation on a stylometric level has not yet been achieved by professional writers and does not appear to be one of the abilities of savants either, whose left hemisphere, including speech and language, is the least developed, which is why such genuine talent cannot be assumed in mediums either. Due to its undeniable successes, its quantitative and computational approach, and its potential to investigate a wide range of research questions, stylometry therefore appears to be a desirable auxiliary science for mediumship research.

S2 Open Access 2023
Improving the performance of cryogenic calorimeters with nonlinear multivariate noise cancellation algorithms

K. Vetter, M. Beretta, C. Capelli et al.

State-of-the-art physics experiments require high-resolution, low-noise, and low-threshold detectors to achieve competitive scientific results. However, experimental environments invariably introduce sources of noise, such as electrical interference or microphonics. The sources of this environmental noise can often be monitored by adding specially designed “auxiliary devices” (e.g. microphones, accelerometers, seismometers, magnetometers, and antennae). A model can then be constructed to predict the detector noise based on the auxiliary device information, which can then be subtracted from the true detector signal. Here, we present a multivariate noise cancellation algorithm which can be used in a variety of settings to improve the performance of detectors using multiple auxiliary devices. To validate this approach, we apply it to simulated data to remove noise due to electromagnetic interference and microphonic vibrations. We then employ the algorithm to a cryogenic light detector in the laboratory and show an improvement in the detector performance. Finally, we motivate the use of nonlinear terms to better model vibrational contributions to the noise in thermal detectors. We show a further improvement in the performance of a particular channel of the CUORE detector when using the nonlinear algorithm in combination with optimal filtering techniques.

8 sitasi en Physics
S2 Open Access 2023
A study on the Side Effects of Chemotherapy and Adjuvant Treatment

Shao-zeng Zhang

Cancer is the second leading cause of death in the world. Globally, nearly one-sixth of deaths are caused by cancer. Cancer raise wide attention from the researcher in recent years, chemotherapy is the main cancer treatment, but chemotherapy has many side effects, the common effects are weight loss, hair loss, and figure. These side effects will bring many pains for patients, as result, sciences made a research to reduce the suffering for patients. In this article, the research method of archival research is used to retrieve the relevant research on the side effects of chemotherapy and adjuvant chemotherapy. By reviewing the previous literature on chemotherapy, we can understand the history, classification, and mechanism of chemotherapy. Through the relevant research on chemical side effects and side effect adjuvant therapy, the following conclusions can be drawn. The common side effects of chemotherapy are bone marrow suppression, gastrointestinal adverse reactions, neutrotoxicity, hepatotoxicity, and easily overlooked taste change. The adjuvant treatments are Chinese herbal therapy, physical therapy, trace element therapy, and psychological therapy. It provides a basis for future medical researchers to study the side effects and auxiliary treatment of chemotherapy, and non-medical researchers can have a clear understanding of chemotherapy. At the same time, it provides a research direction of possibility and increases the interest for my future study.

DOAJ Open Access 2023
Coastal and Inland subsistence strategies during the Gravettian in the Cantabrian Region (northern Iberian Peninsula)

Angel Blanco-Lapaz, Ana B. Marín-Arroyo, Igor Gutiérrez-Zugasti et al.

The Gravettian site of Fuente del Salín Cave is located in the northern Iberian Peninsula (Cantabrian Region, Spain). In 2016, excavations conducted at the site yielded numerous faunal remains, including mammals and fish. Researchers also recovered molluscs remains during the 1990, 1991, and 2000 campaigns. This study presents the results of the archaeozoological and taphonomical analyses of the large mammals and fish remains recovered during these excavations. At Fuente del Salín Cave, a high level of bone fragmentation and the presence of burning damage characterizes the faunal assemblage. Taxonomy of large game shows diverse habitat exploitation, such as coastal valleys and plains (red deer, Cervus elaphus; horse, Equus ferus) as well as rocky landscapes (Iberian ibex, Capra pyrenaica; and chamois, Rupicapra rupicapra). Additionally, fish remains show the presence of salmonids (Salmo sp.), cyprinids, and haddock (Melanogrammus aeglefinus), indicating the exploitation of coastal, demersal, and riverine species. The taphonomic analysis of the mammals revealed burning damage concentrated on axial elements and epiphyses, characterized by temperatures typical of carbonization or calcification. The scarcity of carnivore gnawing marks and the presence of butchering marks prove that humans were the main accumulators at Fuente del Salín Cave. Concerning fish, most remains correspond to spines, branchial spines, ribs, and vertebrae, indicating humans processed fish by removing these elements for consumption. Based on our results, Fuente del Salín Cave provides an essential record of the subsistence strategies of the inhabitants of the Cantabrian Region during the Gravettian.

Geography. Anthropology. Recreation, Archaeology
S2 Open Access 2023
Artificial intelligect and human nature

L. Griffen, N. Ryzheva

The article presents the main transformational processes of scientific understanding of the role and interaction of «artificial» and «natural» intellect. The aim of the work is to analyze and summarize the most promising results of modern scientific research in the context of supporting the priority of solving «intellectual» problems by «natural intellect» and the role of «artificial intellect». Attention is drawn to the specifics of the artificial intellect functioning, which is significantly limited by the fundamental incompleteness of semantic information and the absence of axiological information. The article is based on the data of scientific developments in the field of natural and historical sciences. The natural intellect is presented as a social phenomenon that arose from the interaction of individual cerebral structures, the optimal characteristics of which were formed as a result of previous evolution, and therefore these characteristics allow to understand and make any dominant decisions. The material summarized in the article allowed to formulate some conclusions. In particular, that an artificial non-biological structure cannot be «smarter» than a person – regardless of how quickly and according to what programmes it processes the information, since it does not have its own goals. But the artificial intellect is a powerful tool for society in its interaction with the outside world. A tool – but in no case an agent that determines its goals and objectives. The artificial intellect as an auxiliary means is and will be created in future by people to achieve the same goal that «natural» intellect serves – better possible satisfaction of the needs of society as a whole and the individual in particular.

S2 Open Access 2023
Sociological Trilogy of Cultural Community on Exposure Peril of Monsoon in Western Rural and Urban, Sierra Leone

Mohamed Bangura

Although there are crowds of cultural comprehension and interpretations, the mass of Sierra Leonean sociologists would consent that the anchor of Sierra Leonean sociology is the swotting of personage reciprocation (Bangura, 2019). Consequently, when exposure pounds, Sierra Leonean sociologists have asked, “how do Sierra Leoneans reciprocate?” The natural social standard of intermittence or frequency and ferocity of hydro-meteorological exposures stirring Western Rural and Urban is socially augmenting, preeminent to tough shivers shocks on socio-economic, educational and cultural growth (Jackson, 2023; Wouter Botzen et al, 2019). This social research pinpoints at charting the social judgements of the illustrative social history on the shock of cultural community-predicated social arteries to exposure foresight to the peril of typhoon activated monsoon in Western Rural and Urban, Sierra Leone. The judgements of this social research might bolster link social lacunae in cultural community foresight and imminent social antiphon to the peril of typhoon monsoon, consequently abridging social susceptibility and exposure. Fundamental dossier for social research was captured via online partially-structured social dialogues. Seventy-eight social research respondents were affianced for a profound sociological comprehension of the shock of cultural community predicated arteries on exposure foresight. Auxiliary dossier was collected via social evidence inquiry by scanning, appraising and reviewing briefs and inferences from previous social research discourses, monographs and peer reviewed journals. The Estimate-Talk-Estimate social technique was utilised to assess the impact of cultural community based arteries to exposure foresight. The extensive social research judgement was that cultural community-accustomed practice has a substantially lofty shock on individual threshold of exposure foresight. The social research suggested policy makers to proliferate the social accomplishment of cultural community accustomed exposure oversight practice blueprints as the maximum reasonable scheme to diminish the shocks of typhoon activated monsoons in Western Rural and Urban, Sierra Leone.

CrossRef Open Access 2023
Improvement of Auxiliary Diagnosis of Diabetic Cardiovascular Disease Based on Data Oversampling and Deep Learning

Weiming Yang, Yujia Guo, Yuliang Liu

Diabetic cardiovascular disease is a common complication of diabetes, which can lead to high-mortality diseases such as diabetic cardiomyopathy and atherosclerosis in serious cases. Therefore, effective prevention and management of diabetic cardiovascular disease is demanded. Clinical medical data officers are faced with a situation of a small amount of data and uneven data distribution. In this paper, we propose data oversampling synthesis techniques based on weight and extension algorithms. It can combine 1D-convolutional neural networks and long short-term memory neural networks to solve the problem of a lack of original data. First of all, a few samples based on feature weight are synthesized to make the original unbalanced data evenly distributed. Secondly, the original data are extended and corrected to expand the number of samples. Finally, the deep learning algorithm is used to extract features and classify whether the data have diabetic cardiovascular disease. Data synthesis based on weight and extension algorithms was evaluated on the actual medical datasets and obtained an accuracy of 93.53% and specificity of 94.37%, which confirms that it is an improved solution compared to the other algorithms. Hence, this paper contributes not only a substantial saving of human resources but also improves the efficiency of the clinical diagnosis of diabetic cardiovascular disease, which is conducive to the early detection and treatment of diseases.

S2 Open Access 2020
Use of Remote Sensing Data to Improve the Efficiency of National Forest Inventories: A Case Study from the United States National Forest Inventory

A. Lister, Hans-Eric Andersen, T. Frescino et al.

Globally, forests are a crucial natural resource, and their sound management is critical for human and ecosystem health and well-being. Efforts to manage forests depend upon reliable data on the status of and trends in forest resources. When these data come from well-designed natural resource monitoring (NRM) systems, decision makers can make science-informed decisions. National forest inventories (NFIs) are a cornerstone of NRM systems, but require capacity and skills to implement. Efficiencies can be gained by incorporating auxiliary information derived from remote sensing (RS) into ground-based forest inventories. However, it can be difficult for countries embarking on NFI development to choose among the various RS integration options, and to develop a harmonized vision of how NFI and RS data can work together to meet monitoring needs. The NFI of the United States, which has been conducted by the USDA Forest Service’s (USFS) Forest Inventory and Analysis (FIA) program for nearly a century, uses RS technology extensively. Here we review the history of the use of RS in FIA, beginning with general background on NFI, FIA, and sampling statistics, followed by a description of the evolution of RS technology usage, beginning with paper aerial photography and ending with present day applications and future directions. The goal of this review is to offer FIA’s experience with NFI-RS integration as a case study for other countries wishing to improve the efficiency of their NFI programs.

85 sitasi en Geography
S2 Open Access 2022
Multiple Streams Framework as Scientific Research Program and Tool for the Analysis of Public Policy Issues

František Ochrana, V. Novotný, Olga Angelovská

Abstract The Multiple Streams Framework (MSF) is currently one of the most widely used frameworks in policy process research. It explains how policy agenda develops in the policy process with emphasis on policy adoption. This article examines MSF from the perspective of the history of science and Lakatos’ methodology of scientific research programs. In this respect, we consider MSF a “semi-strong theory” that uses a form of subsumption under theory for scientific explanation. This differs from a “strong” explanatory theory (e.g., physics), which uses explanations in the form of subsumption under scientific law. From the point of view of Lakatos’ methodology, MSF represents a scientific research program. The basic element is a hard core given mainly by the MSF hypothesis for the framework as a whole and MSF assumptions and key structural elements. Around the hard core there is a protective belt of auxiliary hypotheses. They correspond to hypotheses related to the framework’s key elements and to the hypothesis for the framework as a whole. MSF has negative heuristics (prohibition of the use of the modus tollens rule) and positive heuristics, which are represented by a set of theoretically and empirically progressive theories that further develop the MSF research program. An analysis of studies on MSF reveals that single hypotheses are only exceptionally tested using “hard” data and quantitative methods. Qualitative analysis (case studies) predominates, which contributes to the development of MSF theory. Therefore, we can consider a progressive shift in theory. In this respect, MSF can be considered a successful research program. However, in terms of the methodology of scientific research programs, MSF has a number of other opportunities to develop hypothesis testing further and use various modelling methods with data sets. Thus, MSF represents an interesting scientific research program, which needs to be further developed and specified in the spirit of the methodology of scientific research programs, It is a challenge for interdisciplinary research in the field of social sciences.

6 sitasi en
DOAJ Open Access 2022
Settlement patterns in the eastern and central Slovenia during the Middle and the Late Bronze age (the Oloris – Podsmreka horizon)

Brina Škvor Jernejčič, Elena Leghissa, Barbara Brezigar

The article represents a short synthesis about settlements from the Middle and the beginning of the Late Bronze Age in central and eastern regions of Slovenia. Special attention is given to two key-sites, Medvode-Svetje and Trata near Škofja Loka, both from the Gorenjska region. Numerous archaeological excavations and subsequent research publications in the last two decades brought about new insights on dwelling features, forms of housing and on settlement characteristics itself. At the same time, a completely new understanding of the spectre of ceramic repertoire has been obtained, while new radiocarbon dating results enable us to complement the absolute chronology of the given time period.

Archaeology, History of Italy
S2 Open Access 2021
Evolution of Ycf54-independent chlorophyll biosynthesis in cyanobacteria

Guangyu E. Chen, A. Hitchcock, J. Mareš et al.

Significance Photosynthesis uses chlorophylls to utilize solar energy. In oxygenic phototrophs, formation of the isocyclic fifth ring of chlorophyll, responsible for its green color, is catalyzed by AcsF/CycI and the auxiliary protein Ycf54. Removal of Ycf54 causes severe chlorophyll deficiency and impaired photoautotrophic growth. We analyzed laboratory-evolved suppressor mutants of a Ycf54-less strain of the cyanobacterium Synechocystis where chlorophyll biosynthesis and phototrophy were restored. A single point mutation in CycI significantly weakens its dependence on Ycf54, mimicking natural evolution of the enzyme in marine cyanobacteria that lack Ycf54. A second mutation resulting in overaccumulation of chlorophyll inactivates an enzyme with in vitro chlorophyll dephytylase activity. Our results provide insights into the important regulatory role of Ycf54 in chlorophyll biosynthesis. Chlorophylls (Chls) are essential cofactors for photosynthesis. One of the least understood steps of Chl biosynthesis is formation of the fifth (E) ring, where the red substrate, magnesium protoporphyrin IX monomethyl ester, is converted to the green product, 3,8-divinyl protochlorophyllide a. In oxygenic phototrophs, this reaction is catalyzed by an oxygen-dependent cyclase, consisting of a catalytic subunit (AcsF/CycI) and an auxiliary protein, Ycf54. Deletion of Ycf54 impairs cyclase activity and results in severe Chl deficiency, but its exact role is not clear. Here, we used a Δycf54 mutant of the model cyanobacterium Synechocystis sp. PCC 6803 to generate suppressor mutations that restore normal levels of Chl. Sequencing Δycf54 revertants identified a single D219G amino acid substitution in CycI and frameshifts in slr1916, which encodes a putative esterase. Introduction of these mutations to the original Δycf54 mutant validated the suppressor effect, especially in combination. However, comprehensive analysis of the Δycf54 suppressor strains revealed that the D219G-substituted CycI is only partially active and its accumulation is misregulated, suggesting that Ycf54 controls both the level and activity of CycI. We also show that Slr1916 has Chl dephytylase activity in vitro and its inactivation up-regulates the entire Chl biosynthetic pathway, resulting in improved cyclase activity. Finally, large-scale bioinformatic analysis indicates that our laboratory evolution of Ycf54-independent CycI mimics natural evolution of AcsF in low-light–adapted ecotypes of the oceanic cyanobacteria Prochlorococcus, which lack Ycf54, providing insight into the evolutionary history of the cyclase enzyme.

12 sitasi en Medicine

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