Hasil untuk "cs.DB"

Jiali Wang, Yuanyuan li, Fangfang Cao et al.

Abstract Obesity is an independent risk factor for the development of diabetic nephropathy (DN). Obesity, type 2 diabetes, and kidney damage interact with each other. Recent studies have shown that adipokines are closely related to the occurrence and development of DN, and are potential targets for treatment and prevention of complications. However, the potential interaction between adipocytokines and renal injury remains unclear. We investigated the mechanisms linking adipose tissue to DN. We assessed fat and renal functions in db/db and wild-type mice, and the effects of adipocyte-conditioned medium on cultured tubular epithelial cells. Mice were used to analyze changes in plasma BUN, creatinine, urine protein, and renal histology. Additionally, tubular epithelial cells were incubated with adipocyte-conditioned medium to investigate the effects on tubular epithelial cell apoptosis.

SA Nørgaard, FW Sand, DB Sørensen et al.

AbstractIn mouse (Mus musculus) models of diabetic nephropathy (DN), one of the most important read-outs is the 24-h urinary albumin excretion (UAE). The 24-h urine collection is usually performed by single housing mice in metabolic cages on wire mesh without enrichment. This is known to be stressful for the mice. Therefore, it was investigated if shorter urine collections would be sufficient to get reliable assessments of albuminuria. Twenty-one diabetic (C57BLKS-Leprdb/ db) and ten non-diabetic mice (C57BLKS-Leprdb/+) were placed in metabolic cages at 15 and 20 weeks of age (WoA) for 24 h. Urine samples were taken at 4, 6, 18 and 24 h and albumin and creatinine concentration were measured. Four- and 6-h UAE was found to correlate significantly with 24-h UAE. Furthermore, a significant correlation was found between 24-h UAE and albumin:creatinine ratio (ACR) in the 4-h sample. However, the strength of the correlation between ACR and 24-h UAE was weaker than between the 4- and 24-h UAE. This suggests that normalising to creatinine may not provide additional value to the 4-h urine collection. In conclusion, the strong correlation between 4- and 6-h UAE and 24-h UAE indicates that the collection period can be considerably reduced. This refinement could reduce stress and increase welfare of the db/db model and potentially be applied to other DN models.

Manish Sharma

Hui Li, Yu-Qing Bai, Jun-Ya Xie et al.

Bai Song, Yong Fan

Aubin Moutal

Hideharu Abe

T. Aral, T. Itakura

M. Gibbs

M.J. Pechan, Jiangsheng Xu, D.M. Kelly et al.