Semantic Scholar Open Access 2023 71 sitasi

An oncogenic phenoscape of colonic stem cell polarization.

X. Qin F. Cardoso Rodriguez J. Sufi P. Vlckova Jeroen Claus +1 lainnya

Lihat Sumber DOI

Abstrak

Cancer cells are regulated by oncogenic mutations and microenvironmental signals, yet these processes are often studied separately. To functionally map how cell-intrinsic and cell-extrinsic cues co-regulate cell fate, we performed a systematic single-cell analysis of 1,107 colonic organoid cultures regulated by (1) colorectal cancer (CRC) oncogenic mutations, (2) microenvironmental fibroblasts and macrophages, (3) stromal ligands, and (4) signaling inhibitors. Multiplexed single-cell analysis revealed a stepwise epithelial differentiation phenoscape dictated by combinations of oncogenes and stromal ligands, spanning from fibroblast-induced Clusterin (CLU)+ revival colonic stem cells (revCSCs) to oncogene-driven LRIG1+ hyper-proliferative CSCs (proCSCs). The transition from revCSCs to proCSCs is regulated by decreasing WNT3A and TGF-β-driven YAP signaling and increasing KRASG12D or stromal EGF/Epiregulin-activated MAPK/PI3K flux. We find that APC loss and KRASG12D collaboratively limit access to revCSCs and disrupt stromal-epithelial communication-trapping epithelia in the proCSC fate. These results reveal that oncogenic mutations dominate homeostatic differentiation by obstructing cell-extrinsic regulation of cell-fate plasticity.

Topik & Kata Kunci

Medicine

Penulis (6)

X. Qin

F. Cardoso Rodriguez

J. Sufi

P. Vlckova

Jeroen Claus

C. Tape

Format Sitasi

APA MLA BibTeX

Qin, X., Rodriguez, F.C., Sufi, J., Vlckova, P., Claus, J., Tape, C. (2023). An oncogenic phenoscape of colonic stem cell polarization.. https://doi.org/10.1016/j.cell.2023.11.004

Akses Cepat

Lihat di Sumber doi.org/10.1016/j.cell.2023.11.004

Informasi Jurnal

Tahun Terbit: 2023
Bahasa: en
Total Sitasi: 71×
Sumber Database: Semantic Scholar
DOI: 10.1016/j.cell.2023.11.004
Akses: Open Access ✓