Semantic Scholar Open Access 1985 232 sitasi

pH‐sensitive liposomes mediate cytoplasmic delivery of encapsulated macromolecules

R. Straubinger N. Düzgüneş D. Papahadjopoulos

Lihat Sumber DOI

Abstrak

Negatively charged liposomes are endocytosed by the coated vesicle system and accumulate in acidic intracellular vesicles. Liposomes that become unstable at acidic pH improve cytoplasmic delivery of membraneimpermeant macromolecules such as calcein (CAL) and FITC dextran (18 or 40 kDa). Oleic acid (OA): phosphatidylethanolamine (PE) (3:7 mole ratio) liposomes become permeable to CAL at pH < 7.0. Control liposomes of phosphatidylserine : PE or OA : phosphatidylcholine are stable at pH 4–8. OA:PE liposomes promote cytoplasmic delivery of encapsulated CAL to CV‐1 cells, as evidenced by the emergence of diffuse, cytoplasmic CAL fluorescence. Delivery requires metabolic energy and is partially inhibited by chloroquine or monensin, which raise the pH of intracellular vesicles.

Topik & Kata Kunci

Chemistry Medicine

Penulis (3)

R. Straubinger

N. Düzgüneş

D. Papahadjopoulos

Format Sitasi

APA MLA BibTeX

Straubinger, R., Düzgüneş, N., Papahadjopoulos, D. (1985). pH‐sensitive liposomes mediate cytoplasmic delivery of encapsulated macromolecules. https://doi.org/10.1016/0014-5793(85)80210-6

Akses Cepat

Lihat di Sumber doi.org/10.1016/0014-5793(85)80210-6

Informasi Jurnal

Tahun Terbit: 1985
Bahasa: en
Total Sitasi: 232×
Sumber Database: Semantic Scholar
DOI: 10.1016/0014-5793(85)80210-6
Akses: Open Access ✓