Semantic Scholar Open Access 2018 427 sitasi

Imaging cellular ultrastructures using expansion microscopy (U-ExM)

D. Gambarotto Fabian Zwettler M. Le Guennec Marketa Schmidt-Cernohorska Denis Fortun +9 lainnya

Lihat Sumber DOI

Abstrak

Determining the structure and composition of macromolecular assemblies is a major challenge in biology. Here we describe ultrastructure expansion microscopy (U-ExM), an extension of expansion microscopy that allows the visualization of preserved ultrastructures by optical microscopy. This method allows for near-native expansion of diverse structures in vitro and in cells; when combined with super-resolution microscopy, it unveiled details of ultrastructural organization, such as centriolar chirality, that could otherwise be observed only by electron microscopy. U-ExM enables near-native expansion microscopy of samples in vitro and in cells. The combination of U-ExM with confocal microscopy and HyVolution revealed details of centriole chirality that were previously accessible only by electron microscopy.

Topik & Kata Kunci

Medicine Materials Science

Penulis (14)

D. Gambarotto

Fabian Zwettler

M. Le Guennec

Marketa Schmidt-Cernohorska

Denis Fortun

S. Borgers

Jörn Heine

Jan-Gero Schloetel

M. Reuss

M. Unser

E. Boyden

M. Sauer

V. Hamel

P. Guichard

Format Sitasi

APA MLA BibTeX

Gambarotto, D., Zwettler, F., Guennec, M.L., Schmidt-Cernohorska, M., Fortun, D., Borgers, S. et al. (2018). Imaging cellular ultrastructures using expansion microscopy (U-ExM). https://doi.org/10.1038/s41592-018-0238-1

Akses Cepat

Lihat di Sumber doi.org/10.1038/s41592-018-0238-1

Informasi Jurnal

Tahun Terbit: 2018
Bahasa: en
Total Sitasi: 427×
Sumber Database: Semantic Scholar
DOI: 10.1038/s41592-018-0238-1
Akses: Open Access ✓