Semantic Scholar Open Access 2018 325 sitasi

The CryoEM Method MicroED as a Powerful Tool for Small Molecule Structure Determination

Christopher G. Jones M. Martynowycz J. Hattne Tyler J Fulton B. Stoltz +3 lainnya

Lihat Sumber DOI

Abstrak

In the many scientific endeavors that are driven by organic chemistry, unambiguous identification of small molecules is of paramount importance. Over the past 50 years, NMR and other powerful spectroscopic techniques have been developed to address this challenge. While almost all of these techniques rely on inference of connectivity, the unambiguous determination of a small molecule’s structure requires X-ray and/or neutron diffraction studies. In practice, however, X-ray crystallography is rarely applied in routine organic chemistry due to intrinsic limitations of both the analytes and the technique. Here we report the use of the electron cryo-microscopy (cryoEM) method microcrystal electron diffraction (MicroED) to provide routine and unambiguous structural determination of small organic molecules. From simple powders, with minimal sample preparation, we could collect high-quality MicroED data from nanocrystals (∼100 nm, ∼10–15 g) resulting in atomic resolution (<1 Å) crystal structures in minutes.

Topik & Kata Kunci

Materials Science Medicine

Penulis (8)

Christopher G. Jones

M. Martynowycz

J. Hattne

Tyler J Fulton

B. Stoltz

José A. Rodríguez

H. Nelson

T. Gonen

Format Sitasi

APA MLA BibTeX

Jones, C.G., Martynowycz, M., Hattne, J., Fulton, T.J., Stoltz, B., Rodríguez, J.A. et al. (2018). The CryoEM Method MicroED as a Powerful Tool for Small Molecule Structure Determination. https://doi.org/10.1021/acscentsci.8b00760

Akses Cepat

Lihat di Sumber doi.org/10.1021/acscentsci.8b00760

Informasi Jurnal

Tahun Terbit: 2018
Bahasa: en
Total Sitasi: 325×
Sumber Database: Semantic Scholar
DOI: 10.1021/acscentsci.8b00760
Akses: Open Access ✓