Semantic Scholar Open Access 2009 718 sitasi

Super-Resolution Video Microscopy of Live Cells by Structured Illumination

P. Kner Bryant B. Chhun E. Griffis Lukman Winoto M. Gustafsson

Lihat Sumber DOI

Abstrak

Structured-illumination microscopy can double the resolution of the widefield fluorescence microscope but has previously been too slow for dynamic live imaging. Here we demonstrate a high-speed structured-illumination microscope that is capable of 100-nm resolution at frame rates up to 11 Hz for several hundred time points. We demonstrate the microscope by video imaging of tubulin and kinesin dynamics in living Drosophila melanogaster S2 cells in the total internal reflection mode.

Topik & Kata Kunci

Materials Science Medicine

Penulis (5)

P. Kner

Bryant B. Chhun

E. Griffis

Lukman Winoto

M. Gustafsson

Format Sitasi

APA MLA BibTeX

Kner, P., Chhun, B.B., Griffis, E., Winoto, L., Gustafsson, M. (2009). Super-Resolution Video Microscopy of Live Cells by Structured Illumination. https://doi.org/10.1038/nmeth.1324

Akses Cepat

Lihat di Sumber doi.org/10.1038/nmeth.1324

Informasi Jurnal

Tahun Terbit: 2009
Bahasa: en
Total Sitasi: 718×
Sumber Database: Semantic Scholar
DOI: 10.1038/nmeth.1324
Akses: Open Access ✓