Semantic Scholar Open Access 2010 1840 sitasi

Mediator and Cohesin Connect Gene Expression and Chromatin Architecture

M. Kagey J. Newman S. Bilodeau Y. Zhan David A. Orlando +8 lainnya

Lihat Sumber DOI

Abstrak

Transcription factors control cell-specific gene expression programs through interactions with diverse coactivators and the transcription apparatus. Gene activation may involve DNA loop formation between enhancer-bound transcription factors and the transcription apparatus at the core promoter, but this process is not well understood. Here we report that mediator and cohesin physically and functionally connect the enhancers and core promoters of active genes in murine embryonic stem cells. Mediator, a transcriptional coactivator, forms a complex with cohesin, which can form rings that connect two DNA segments. The cohesin-loading factor Nipbl is associated with mediator–cohesin complexes, providing a means to load cohesin at promoters. DNA looping is observed between the enhancers and promoters occupied by mediator and cohesin. Mediator and cohesin co-occupy different promoters in different cells, thus generating cell-type-specific DNA loops linked to the gene expression program of each cell.

Topik & Kata Kunci

Medicine Biology

Penulis (13)

M. Kagey

J. Newman

S. Bilodeau

Y. Zhan

David A. Orlando

Nynke L. van Berkum

Christopher C. Ebmeier

Jesse Goossens

P. Rahl

S. Levine

D. Taatjes

J. Dekker

R. Young

Format Sitasi

APA MLA BibTeX

Kagey, M., Newman, J., Bilodeau, S., Zhan, Y., Orlando, D.A., Berkum, N.L.v. et al. (2010). Mediator and Cohesin Connect Gene Expression and Chromatin Architecture. https://doi.org/10.1038/nature09380

Akses Cepat

Lihat di Sumber doi.org/10.1038/nature09380

Informasi Jurnal

Tahun Terbit: 2010
Bahasa: en
Total Sitasi: 1840×
Sumber Database: Semantic Scholar
DOI: 10.1038/nature09380
Akses: Open Access ✓